BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,315	G→A	58.4%	Y79Y (TAC→TAT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,315	0	G	A	58.4%	268.3 / inf	904	Y79Y (TAC→TAT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (175/192); new base A (247/272); total (437/467)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.23e-01

Only 100 of 907 total aligned reads displayed.

ACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTC  >  CP000730/36177‑36455

aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATAAATGGCACGATATAAATAGACCCATTTTCCTTTTATTTTGATGtt                                                                                                                                            >  1:767336/1‑140 (MQ=12)

 ccAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTGGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATGAATGGCACGAGATAAATAGCTCCATTGTCTTTTTTTTTTTATTTAc                                                                                                                                           >  1:78092/1‑141 (MQ=11)

  cAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCTCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACg                                                                                                                                          >  2:347539/1‑141 (MQ=12)

       tGTTCAATGAGACGTTGGCTATACGCATATGCTTAATGATTTTCTCGTTGCGTACGCAACCAAAGATCTAATGTATGGCCCTCTGCAGCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtca                                                                                                                                     <  2:400046/141‑1 (MQ=11)

        ttttAATGAGTCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcat                                                                                                                                    <  1:422590/140‑1 (MQ=255)

        ttttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                                                                    <  2:604454/140‑1 (MQ=17)

        gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                                                                    <  1:190530/141‑1 (MQ=32)

         tttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTACGTCtcatc                                                                                                                                   >  1:297319/1‑141 (MQ=17)

            aatgactagTTTTATAAACGTATATGTTGAATGATTATCTCGTTCCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                                                                <  1:501568/133‑1 (MQ=11)

            aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTACTGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCCTTTTCCTTTTATTTTGATGTACGTTTCATCGAt                                                                                                                                >  2:481173/1‑141 (MQ=255)

            aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                                                                <  1:536840/141‑1 (MQ=12)

             aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATa                                                                                                                               >  2:839488/1‑141 (MQ=17)

             aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATa                                                                                                                               <  2:728073/141‑1 (MQ=12)

             aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCC‑TTTATTTTGATGTACGTCTCATCAATAc                                                                                                                              <  2:756841/141‑1 (MQ=17)

              tGAGCCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCGCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                                                              <  1:812331/141‑1 (MQ=255)

              tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                                                              <  2:381685/141‑1 (MQ=17)

              tGAGAAGTTCGATAGACGTCGGTGTTGAATGGTTAGCTTGTTTCTTACGCAACCAAATAGCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAc                                                                                                                              <  2:861103/141‑1 (MQ=11)

              agagaCTTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTTTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                                                              <  2:51025/140‑1 (MQ=11)

                agaCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGc                                                                                                                            <  1:755276/141‑1 (MQ=12)

                     tttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACCTCTCATCAGTCCGGCAttt                                                                                                                       >  1:444228/1‑141 (MQ=11)

                      ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCGTCAATACGCCAttta                                                                                                                      >  1:89018/1‑140 (MQ=12)

                      ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTg                                                                                                                      >  2:360598/1‑141 (MQ=17)

                         aTAAATGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaa                                                                                                                   <  2:666582/141‑1 (MQ=17)

                           aagcGCCTATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaata                                                                                                                 <  1:64428/138‑1 (MQ=17)

                           aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaata                                                                                                                 >  1:909526/1‑141 (MQ=12)

                           aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCTTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTGTACGTCTCTTCAATACTCCCTTTGtataa                                                                                                                 >  1:175230/1‑138 (MQ=11)

                                cATATGCTGAAGGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCCCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtt                                                                                                            <  1:100644/141‑1 (MQ=255)

                                    tGCTGAATGATTATCTCGTTGCTTACGCAACCCAGTATCTAATGTGTTTATCGCGGGATCGATGGCACGATATAAATAGCTTCATTTTCCTTTTATTTTGATATACGTCTCATCCATACGCCATTTGTAATAAGCtttttt                                                                                                        >  2:643278/1‑141 (MQ=11)

                                    tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTTTATTAATGGCACGTTTTAAATAGCTCCATTTTGCTTTTATTTTGTTGTTCTTCTCATTAGTACGCCATTTGTAATATGGtttttt                                                                                                        >  1:251813/1‑141 (MQ=11)

                                      cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAAGCCATTTGTAATAAACttttgttt                                                                                                      >  1:203469/1‑137 (MQ=11)

                                      cTGAATGATTACATCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCCCTGTATCAACGGCCCGAGATAAATGACACCCTTTTCCTTTTAGTTGTATATACGCCACATAAATAGGCCATTTGTAATAAGCTTTTTTAt                                                                                                      >  2:89261/1‑141 (MQ=2)

                                       tGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCACTCTGTATCAATGGCACGATATAAATAGCTCCCTTTTCCTTTGCTTTTGATATACGTCTCATCAATACGCCCTTTGTAGTAAGCTTTTTTAtc                                                                                                     >  2:595185/1‑140 (MQ=11)

                                       tGAATGATTATCTCGTTGCTTAAGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTAATATACGTCTCATCAATAGACGATTTGTAATAAGCTTTTTTAtt                                                                                                     >  2:187802/1‑140 (MQ=11)

                                             aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTCATAAGCTTTTTTATGCttttt                                                                                               >  2:128700/1‑141 (MQ=255)

                                             aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAATTAGCTCCAGTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTagtgttttt                                                                                               >  2:829658/1‑133 (MQ=255)

                                              ttATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGGtttttt                                                                                              >  2:266081/1‑140 (MQ=255)

                                               tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                             >  1:840612/1‑141 (MQ=255)

                                                aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTATAATAAACTTTTTTATGCTTTttctt                                                                                            >  2:711498/1‑141 (MQ=255)

                                                  ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCGCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCGATACGCCAGTTGGGTTAATCTTTTTTATGCTTTCTCTTcc                                                                                          <  1:670462/141‑1 (MQ=14)

                                                     gTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaaa                                                                                       >  2:162961/1‑141 (MQ=255)

                                                     gTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaaa                                                                                       >  1:96488/1‑141 (MQ=40)

                                                       tGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAtt                                                                                     <  1:342783/141‑1 (MQ=255)

                                                        gCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATTGCTCCATTTTTCTTTTATTTTGATATACGTCTCATCAATACGTTATTTGTAATAGGCTGTCTTATGCTTTTTGTTTCattttt                                                                                    >  1:47677/1‑136 (MQ=11)

                                                         cTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAAtttt                                                                                   >  2:105194/1‑140 (MQ=255)

                                                            aCGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTAATCAATACGCCATTTGTAATAAACTTTTGTATGCTTTTTCTTCCAAATTTgttt                                                                                >  1:406424/1‑138 (MQ=255)

                                                             cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAc                                                                               <  2:580751/141‑1 (MQ=40)

                                                              gCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAGGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCTAAATTTGTTata                                                                              >  1:564958/1‑139 (MQ=255)

                                                              gCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACa                                                                              <  1:628517/141‑1 (MQ=40)

                                                               cAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaa                                                                             >  2:529841/1‑141 (MQ=255)

                                                                aaCCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaa                                                                            >  2:433219/1‑141 (MQ=38)

                                                                    aaaTATCTAAAGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTg                                                                        <  2:742889/141‑1 (MQ=255)

                                                                     aaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACTCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                       <  1:589791/141‑1 (MQ=255)

                                                                        atCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGc                                                                    <  1:727993/141‑1 (MQ=255)

                                                                         tCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCTAATTTGATACAAGATTGGGGct                                                                   >  2:312941/1‑140 (MQ=255)

                                                                           tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                                 >  2:246334/1‑141 (MQ=37)

                                                                            aaTGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCatat                                                                <  1:862118/141‑1 (MQ=37)

                                                                                 ttgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                           <  2:682056/140‑1 (MQ=255)

                                                                                 acgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                           <  2:617400/139‑1 (MQ=255)

                                                                                   ttCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGaa                                                         <  1:355937/140‑1 (MQ=21)

                                                                                    tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                        <  1:744810/141‑1 (MQ=255)

                                                                                     cccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTTTAATAAGCTTTTTTATGCTTTTTCTTCATAATTTGATATAAAATTGGTGCATATTCTTTAAcc                                                       >  1:869792/1‑141 (MQ=11)

                                                                                         aagaaTCAAGTGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAAc                                                   >  2:332913/5‑141 (MQ=255)

                                                                                           gtaTCAATGGCACGATATAAATAGCGCCATTTTCCTTTTATTTTGATATACGTCTCATCAATCCGCAAGTTGTAATAAGCTGTCTTATGCTTTTTCTTCCAAATTTGATATACAATTGTGGCAGATTCTTGATACCAACgg                                                 >  2:148706/3‑141 (MQ=11)

                                                                                               cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCAACAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAACATTGTGGCAAATTCTTGAACCCAAAGGTAGa                                             >  2:695563/1‑141 (MQ=11)

                                                                                                aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGat                                            >  1:165808/1‑140 (MQ=255)

                                                                                                 aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGAcc                                           >  1:113853/1‑141 (MQ=39)

                                                                                                    gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGTTATAAAATTGGGGCAGATTCTTGAACCCAACTGTAGATCGtt                                        >  1:374622/1‑141 (MQ=11)

                                                                                                    gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGtt                                        >  2:300099/1‑141 (MQ=255)

                                                                                                      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAGTTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                      >  1:513529/1‑141 (MQ=25)

                                                                                                       cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                     <  1:220755/141‑1 (MQ=255)

                                                                                                         atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                   <  1:321913/141‑1 (MQ=255)

                                                                                                         atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                   <  2:320408/141‑1 (MQ=255)

                                                                                                          tataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatga                                  <  1:265303/141‑1 (MQ=40)

                                                                                                          tataAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatga                                  <  1:834571/141‑1 (MQ=255)

                                                                                                           ataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgat                                 <  1:236069/141‑1 (MQ=40)

                                                                                                             aaaTAGCTCCATTTTCTTTTTTTTTTGATATACGTCTCATCAATACGCAATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTCAACCCAACGGTTTATCGTTGAatgatca                               >  2:801649/1‑139 (MQ=11)

                                                                                                              aaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCACCGGTAGACCGTTGAATGATGaa                              >  1:690763/1‑141 (MQ=255)

                                                                                                              aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                              >  1:604522/1‑141 (MQ=255)

                                                                                                              aaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATGTGTACGAAGCTTTTTTATGCTTTACCTTCAACCATTGTTGTGATGGTGGGGGTTATTCTTGAACCCAACGGCAGATCGTTGAATTATGaa                              >  2:413774/1‑141 (MQ=2)

                                                                                                                   cTCCATTTTCCTTTTATTTTGATATACGTCTCAACAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAGATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGttt                         <  2:814489/141‑1 (MQ=11)

                                                                                                                     ccATTTTCCTTTTATTTTGATATAAGTCTCATCAATACGCCATTTGTAATAAGCTTCTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGTGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTac                       <  2:569129/141‑1 (MQ=11)

                                                                                                                      cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTATATCTTCCAAATTTGATATCAAATTGGCGCATATTCTTGAACCCAACGCTACACCGTTGAATGATGAACGTTTaca                      >  2:227784/1‑141 (MQ=11)

                                                                                                                       tttttCCTTTTATTTTGGTGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                     <  2:206814/140‑1 (MQ=21)

                                                                                                                       aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                     <  1:695907/141‑1 (MQ=255)

                                                                                                                       aTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCACTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                     <  2:463215/141‑1 (MQ=255)

                                                                                                                        ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATTCAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                    >  2:781071/1‑141 (MQ=255)

                                                                                                                        ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                    >  1:395794/1‑141 (MQ=255)

                                                                                                                        ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                    <  2:541947/141‑1 (MQ=255)

                                                                                                                        ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                    >  2:870495/1‑141 (MQ=255)

                                                                                                                         tttCCTTTTATTTTGATATACGTCTCATCAATACGCCCTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGCTATAAAATTGGGGCATATTCCTCAACCCCACCGTAGACCGTTCACTGCTGCACGctttccccc                   >  1:799700/1‑135 (MQ=11)

                                                                                                                         tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                   >  1:712885/1‑141 (MQ=255)

                                                                                                                          ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                  <  1:43964/141‑1 (MQ=18)

                                                                                                                          ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGTGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                  >  2:289995/1‑141 (MQ=255)

                                                                                                                           tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACg                 >  1:15216/1‑141 (MQ=18)

                                                                                                                           tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACAACg                 >  2:908894/1‑141 (MQ=11)

                                                                                                                               tttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATTAACGTTTCCCCCACGTTct             >  2:634326/1‑140 (MQ=11)

                                                                                                                                 tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt           >  1:548164/1‑141 (MQ=21)

                                                                                                                                 tATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTCTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCt           <  1:132918/141‑1 (MQ=37)

                                                                                                                                  aTTTTTATATACGTCCCATCAATACGCCATTTGTAATCAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGTTGCATATTCTTGATCCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt          <  1:36061/141‑1 (MQ=11)

                                                                                                                                     ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat       <  1:308763/141‑1 (MQ=11)

ACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTC  >  CP000730/36177‑36455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`