Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,581 C→T 30.9% intergenic (‑30/‑493) USA300HOU_0026 ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,5810CT30.9% 250.1 / 119.3 168intergenic (‑30/‑493)USA300HOU_0026/USA300HOU_0028IS431mec transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (54/61);  new base T (22/30);  total (77/91)
Fisher's exact test for biased strand distribution p-value = 6.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 168 total aligned reads displayed.

CCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTT  >  CP000730/36443‑36718
                                                                                                                                          |                                                                                                                                         
cccTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACta                                                                                                                                         <  2:807687/141‑1 (MQ=32)
    tAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaaa                                                                                                                                     <  1:827325/141‑1 (MQ=34)
      atatTTCAGATATATCACGATAGCTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGGCTCCTTTTTGTTAAAATTATATTATAAAtt                                                                                                                                   <  2:505577/141‑1 (MQ=255)
        atTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCa                                                                                                                                 >  2:180952/1‑141 (MQ=35)
         tgtccgacatatCACGAGAACTCAAGGATTATCTTAGATAGTAGCCAACAGCTACAGTGATAATATCCTTGTTAAATTGTTTATATCTGAAATAGCTCATACAGAAGACGCCTTTTTGTTAAACTTATACTATAAATTCaa                                                                                                                                <  2:251002/133‑1 (MQ=11)
              gATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTg                                                                                                                           >  1:845402/1‑141 (MQ=35)
              gATATATCACGATAACTCAATGTATATATTATATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTTAAATTATACTATAAATTCAACtttt                                                                                                                           >  2:838110/1‑140 (MQ=255)
               atatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGc                                                                                                                          >  1:844644/1‑141 (MQ=35)
               atatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGc                                                                                                                          <  1:603254/141‑1 (MQ=35)
                tatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCa                                                                                                                         >  2:764390/1‑141 (MQ=34)
                 atatCACGATAACTCAATGTTTATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATCAATTCAACTTTGCaa                                                                                                                        <  1:673840/141‑1 (MQ=21)
                 atatCACGATAACTCAATGTATATCTTAGATAGAAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCaa                                                                                                                        >  1:128668/1‑141 (MQ=25)
                  tatCACGATAACTCCATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc                                                                                                                       <  1:700923/141‑1 (MQ=25)
                  taccACGATAACTCAATGCATATCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAAc                                                                                                                       <  2:358165/138‑1 (MQ=0)
                    tCACGATAGCTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATCCAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAg                                                                                                                     <  1:820189/141‑1 (MQ=255)
                     cACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGa                                                                                                                    >  1:32253/1‑141 (MQ=35)
                      aCGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGaa                                                                                                                   >  1:622792/1‑141 (MQ=35)
                          tagttCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                               <  2:152066/137‑1 (MQ=9)
                          tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                               <  1:191032/138‑1 (MQ=12)
                          tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGCGAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                               >  2:878546/4‑141 (MQ=11)
                          tAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt                                                                                                               >  1:675169/1‑141 (MQ=37)
                          tAACTCAATGAATATCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTAATTCTTCTATAAATTCACCTTTTCACCAGaccgtc                                                                                                               >  2:421477/1‑136 (MQ=11)
                           agcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGATCATACAGAAGACTCCTTTTTGTTAAAATTATAATATAAATTCAACTTTGCAACAGAACCGtc                                                                                                              >  1:612528/3‑140 (MQ=2)
                           agcTCAAAGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGta                                                                                                              <  1:33037/139‑1 (MQ=12)
                            gcttaaTGCATATCTTAGCTAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                             <  1:148706/137‑1 (MQ=255)
                            gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTCTATTATCAATTCAACTTTGCAACAGAACCGtat                                                                                                             >  2:279247/2‑141 (MQ=255)
                            gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                             >  1:357550/2‑141 (MQ=17)
                            aCTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                             >  1:225804/1‑141 (MQ=39)
                             cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                                                                                                            <  1:193331/141‑1 (MQ=21)
                              tcaacgcctatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTGTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt                                                                                                           <  2:155286/133‑2 (MQ=11)
                              tCAATGCATATCTTAGGTAGTGGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt                                                                                                           >  1:465728/1‑140 (MQ=11)
                              tCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt                                                                                                           >  1:496056/1‑140 (MQ=16)
                               cAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtattat                                                                                                          <  2:177978/141‑1 (MQ=40)
                                 aTGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATgg                                                                                                        >  1:425124/1‑141 (MQ=255)
                                  ttcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                       <  2:395794/138‑6 (MQ=255)
                                  agcattttttagttagTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCCTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                       <  1:611092/133‑6 (MQ=11)
                                  agcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgt                                                                                                       <  2:326403/138‑11 (MQ=11)
                                   gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgta                                                                                                      <  2:332651/139‑12 (MQ=11)
                                   gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgta                                                                                                      <  2:560298/139‑12 (MQ=11)
                                   gcatatCATAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgta                                                                                                      <  2:736995/139‑12 (MQ=11)
                                    catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtat                                                                                                     >  2:60833/2‑129 (MQ=11)
                                    catatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactata                                                                                                     <  1:761125/140‑13 (MQ=11)
                                     atatGTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTATATAACTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAAGTCAACTTTGCAACAGAAcatcctaaccgtatt                                                                                                    >  2:796574/1‑127 (MQ=11)
                                     atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGACATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagt                                                                                                    >  2:98120/1‑133 (MQ=255)
                                     atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAAAAGAACCGtattttatgagt                                                                                                    >  2:269609/1‑133 (MQ=11)
                                      tatCTTAGATCGTACCCAACAGTCACAGTGATAACATTCTTGTTAAATTGTCTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatctt                                                                                                   <  2:603420/141‑15 (MQ=11)
                                      tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctactaactggatta                                                                                                   >  2:870795/1‑127 (MQ=11)
                                      tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagta                                                                                                   <  2:233923/141‑10 (MQ=255)
                                      tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAGATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatatg                                                                                                   <  1:249966/141‑15 (MQ=11)
                                      tatCTTAGATAGTAGACAAAGGCTACAGTGATAACATCCTAGTTAAATTGTTTATATCTGACATAGTTCATACAGAAGAATCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAAAACCGtattttatgcgta                                                                                                   >  1:441864/1‑132 (MQ=11)
                                       atCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATaga                                                                                                  >  1:144647/1‑141 (MQ=255)
                                         cTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacac                                                                                                >  2:96322/1‑129 (MQ=11)
                                         cTTAGATAGTAACCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATTCACACCATGGAACTTTGCAACAGAACCGTATTATGGAATAgaga                                                                                                <  1:436656/141‑1 (MQ=14)
                                           tAGATAGTAGCCCACCGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                              <  1:304388/141‑15 (MQ=11)
                                           tAGATAGTAGCCAATGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                              <  1:159158/141‑15 (MQ=11)
                                              aTAGTAGTCAACTGCAACAGTCATAACATCCGTGTTAAATTGTTTATATCTGAAATAGTTCATACAGCAGACTCCCTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGCTGTTg                                                                                           <  2:588108/141‑1 (MQ=17)
                                                  tagCCAACGGTTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGACTAGAGGTGTTgtttt                                                                                       <  2:557581/141‑5 (MQ=255)
                                                  tagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTaa                                                                                       >  2:858601/1‑141 (MQ=255)
                                                      cAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACagt                                                                                   <  2:106846/141‑3 (MQ=255)
                                                        aCGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTta                                                                                 <  2:90200/141‑1 (MQ=255)
                                                        aCGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTta                                                                                 <  1:781071/141‑1 (MQ=255)
                                                         cGGCTACAGTGATAGCATCCTTGTTAAATTGTTTATATCTGAAATAGTGCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtat                                                                                <  1:390889/141‑1 (MQ=255)
                                                         cGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtat                                                                                <  1:420222/141‑1 (MQ=255)
                                                         cGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCAAACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCACCTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtat                                                                                <  1:496599/141‑1 (MQ=255)
                                                         cGGCTACAGTGATAACATCCTTGTTAAATTGTTTATAACTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtat                                                                                <  1:337642/141‑1 (MQ=255)
                                                          ggCTACAGTGATAACATCCTTGTAAAATTGCTTATATCTGAAATAGTTCATCCAGAAGACTACTTTTTGTTAAAATTATACTATATATTCAACTTTGCAAAAGAACCGTATTATGGAATAGAGATGTTGATAACATTtcta                                                                               >  2:136814/1‑141 (MQ=17)
                                                           gCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATAc                                                                              >  2:206801/1‑141 (MQ=255)
                                                             tACGGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAg                                                                            >  1:655580/1‑141 (MQ=255)
                                                                           ccTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTTTTTTTTTTCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTGATATTTATACGGGAtattactacttt                                                              >  2:175246/1‑130 (MQ=18)
                                                                               tttAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCGTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGtt                                                          <  2:717947/140‑1 (MQ=255)
                                                                                    aTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAtt                                                     <  2:225804/141‑1 (MQ=255)
                                                                                        tttATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGt                                                 <  2:631008/141‑1 (MQ=255)
                                                                                          tataTCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAACATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGtta                                               <  1:119040/141‑1 (MQ=255)
                                                                                            tatCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGttatt                                             >  1:794163/1‑141 (MQ=255)
                                                                                            tatCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGttatt                                             >  1:582157/1‑141 (MQ=255)
                                                                                            aatCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGAATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGttatt                                             <  2:1023/140‑1 (MQ=255)
                                                                                             ttCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGttatta                                            <  2:873773/140‑1 (MQ=255)
                                                                                             atCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGttatta                                            <  2:708401/141‑1 (MQ=255)
                                                                                                   aaTAGTTCATACAGAAGACTCCTTTTTTTTTAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAAATGTTGGTAACATTTATACCGGATCATTATACTTAAGTTTAATTTAGTTATTACATAAc                                      >  2:250819/1‑141 (MQ=37)
                                                                                                   aaTAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAAc                                      <  1:180952/141‑1 (MQ=255)
                                                                                                       gTTCATACAGATGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGCAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcaca                                  <  1:572542/141‑1 (MQ=255)
                                                                                                        ttCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcacac                                 >  1:46050/1‑141 (MQ=255)
                                                                                                        ttCATACAGAAGACTCCTTTTTGTTAAAATCATACTGTAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAGTTTCGTTATTACAAAACcaaac                                 >  1:629815/1‑141 (MQ=38)
                                                                                                         tCATACAGAAGACTCCTTTTTTTTTTTTTTTTTCTATAAATTCAACTTTGCAAAAGAACCGAATGCTGGAATAGAGATGTTGGTAACAGTTATACAGGATCATAATACTTAAGTTTATTTTCGttattcatataacacaca                                >  2:447716/1‑128 (MQ=255)
                                                                                                             aCAGAGGACTGCTTTGTGTTAAAATTATACTATAAATTCAGCTTTGCAACAGAAACGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTcc                            <  2:229199/141‑1 (MQ=255)
                                                                                                             aCAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTcc                            <  1:426471/141‑1 (MQ=255)
                                                                                                                gaagaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAAc                         >  2:900328/1‑141 (MQ=255)
                                                                                                                gaagaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACACAACATTCCAAc                         >  2:360207/1‑141 (MQ=255)
                                                                                                                gaagaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAGTTCGTTATTACAGAGGCACACATTCCAAc                         >  1:3272/1‑141 (MQ=255)
                                                                                                                 aagaCTCCTTTTTTTTTTTTTTTTACTATAAATTCAACTTTGCAACAGAACCGTATTATGTAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTTTTTCAGAACCACACATTCCAAcc                        >  2:51234/1‑141 (MQ=255)
                                                                                                                    aCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCaga                     >  1:396819/1‑141 (MQ=255)
                                                                                                                        cTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAagag                 >  1:797309/1‑141 (MQ=255)
                                                                                                                         tttttGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAgaga                <  1:628344/141‑1 (MQ=255)
                                                                                                                          ttttGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGaa               <  1:258617/141‑1 (MQ=255)
                                                                                                                          ttttGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGaa               <  1:858601/141‑1 (MQ=255)
                                                                                                                             tGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGt            <  1:481124/141‑1 (MQ=255)
                                                                                                                               ttAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGCACCACACATTCCAACCAGAAGAGGAAGTAt          >  1:770475/1‑141 (MQ=255)
                                                                                                                                 aaaaTTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTACTTTCGTTATTACAGAACCCCACATTCCACCCAGAAGAGAAAGTATGt        >  1:413183/1‑141 (MQ=255)
                                                                                                                                       ataCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTAttt  >  1:557472/1‑141 (MQ=255)
                                                                                                                                       ataCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCACAAAAGAAAGTATGTCTAgtt  >  2:728274/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                         
CCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTT  >  CP000730/36443‑36718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: