Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,180 C→T 24.6% intergenic (+238/‑896) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,1800CT24.6% 283.6 / 77.1 151intergenic (+238/‑896)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (43/70);  new base T (15/22);  total (58/93)
Fisher's exact test for biased strand distribution p-value = 8.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.11e-01

Only 100 of 151 total aligned reads displayed.

AATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTA  >  CP000730/76047‑76319
                                                                                                                                     |                                                                                                                                           
aaTATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGta                                                                                                                                      >  1:123873/1‑141 (MQ=255)
 atatAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGtat                                                                                                                                     <  1:910552/141‑1 (MQ=255)
  tataACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAAGTATATCAAAATATAAGTCCTGTTGCAAAGTTGAATCTATAGtata                                                                                                                                    >  2:667222/1‑141 (MQ=255)
       cTAATATTGCTGCACCTATATCACTTAAAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAtttt                                                                                                                               <  1:504433/141‑1 (MQ=255)
             ttGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACaaa                                                                                                                         >  1:460796/1‑141 (MQ=255)
                ctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAg                                                                                                                      <  1:751878/141‑1 (MQ=255)
                ctgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAACAACTAGCCTGAAAGTTAGGTATAAGTACAATTTTGAATCTATAGTATAATTTTAACAAAAAg                                                                                                                      <  1:436283/141‑1 (MQ=255)
                cggcATCTTTATCACGTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAACATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAg                                                                                                                      <  1:272370/139‑1 (MQ=255)
                 tgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAgg                                                                                                                     <  1:616003/141‑1 (MQ=255)
                 tgcACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAgg                                                                                                                     <  1:844420/141‑1 (MQ=255)
                 ctcACCTGTATCACTTAGAACAGCTAACCGAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTGAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAgg                                                                                                                     <  2:26401/139‑1 (MQ=255)
                     ccTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtc                                                                                                                 >  2:784179/1‑141 (MQ=255)
                        gTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGtcttc                                                                                                              <  1:104423/141‑1 (MQ=255)
                           tCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAACACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGt                                                                                                           <  1:389916/141‑1 (MQ=255)
                              cTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATg                                                                                                        <  1:145364/141‑1 (MQ=255)
                               ttAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGa                                                                                                       <  1:659753/141‑1 (MQ=255)
                                 aGAACATCTAATCAAAGTTTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGTAGTCTTCTGTATGAAc                                                                                                     <  1:450984/141‑1 (MQ=255)
                                 aGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAAc                                                                                                     <  1:49592/141‑1 (MQ=255)
                                 aGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAATTGAATCCAAAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAAc                                                                                                     >  1:851898/1‑141 (MQ=255)
                                    aCAGCTAACAAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAt                                                                                                  <  1:376567/141‑1 (MQ=255)
                                     cgacTAAGCAAAGTGTTAGTAATCCAGGGTATACTAACACAAAGGAAATTAATTTAATCATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAACCTATAGTATTATTTTACCTACAAGGAGACTTCTGTTTAAACTAtt                                                                                                 >  2:108614/4‑141 (MQ=25)
                                      aGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAttt                                                                                                >  1:571625/1‑141 (MQ=255)
                                      aGCTAACCAAAGGGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTAttt                                                                                                <  1:31884/141‑1 (MQ=255)
                                       gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTc                                                                                               <  1:153313/141‑1 (MQ=255)
                                       gCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATAATAAAAAAAAGGAGTCTTCTGTATGAAGTATTTc                                                                                               <  2:503845/141‑1 (MQ=255)
                                       acTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTGTGTATGAACTATTTc                                                                                               <  1:675396/140‑1 (MQ=255)
                                        cTAACCAAAGTGTTAGTAATCCAAGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCa                                                                                              <  2:723594/141‑1 (MQ=255)
                                              aaaGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGAtata                                                                                        >  1:910243/1‑141 (MQ=255)
                                               aaGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATaa                                                                                       <  1:780422/141‑1 (MQ=255)
                                                     tAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAtt                                                                                 >  1:50702/1‑141 (MQ=255)
                                                      tagaaTCCAAGGAAAAATAACACAATGTCAATGAATTTAATAATTATAACAAAATATAGGTTCTGTTGCAAAGTTTAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAAttt                                                                                <  2:144131/138‑1 (MQ=18)
                                                      aGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCGGTTGCAAAGTTGAATCGATAGTATAAGTTTAACAAAAAGGAGTCTTGTGGCTGAACTCTTTCAGACATAAACAActt                                                                                >  2:822776/1‑141 (MQ=255)
                                                          aTCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACa                                                                            <  2:749913/141‑1 (MQ=255)
                                                            ccAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAg                                                                          <  1:387180/141‑1 (MQ=255)
                                                            acAGGGAATACTAACACAAAGGCATATAATCTAATAATTATAGCAAAATATAGGTTATGTTGCAAATTTGAATCTATAGTATAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAg                                                                          <  2:570290/140‑1 (MQ=21)
                                                             cAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTATGTATGAACTATTTCGGATATAAACAATTTAACAAgg                                                                         >  1:240621/1‑141 (MQ=255)
                                                                  aaTACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATCGGTTCTTTTGCAAAGTTGAATCTATAGTATAATTTTAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGtt                                                                    <  2:675613/141‑1 (MQ=255)
                                                                   aTACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTa                                                                   >  1:251419/1‑141 (MQ=255)
                                                                        aaCACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCGGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACt                                                              <  1:128868/141‑1 (MQ=255)
                                                                         acacAAAGTCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTg                                                             <  1:883162/141‑1 (MQ=255)
                                                                          aacaAAGGCAATTAATTTAATAATTATAGAAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCCGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGt                                                            <  2:193274/140‑1 (MQ=255)
                                                                          aaaaaaGGCAATTAATTTCATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGt                                                            <  1:101040/138‑1 (MQ=255)
                                                                            caAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAg                                                          <  1:201081/141‑1 (MQ=255)
                                                                            caAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTGTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAg                                                          <  2:174002/141‑1 (MQ=255)
                                                                            aaaaGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAg                                                          <  2:514631/140‑1 (MQ=255)
                                                                              aaGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                        <  1:446185/141‑1 (MQ=255)
                                                                              aaGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                        <  1:185766/141‑1 (MQ=255)
                                                                              aaGGAAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGcc                                                        <  2:211683/141‑1 (MQ=255)
                                                                               aGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCg                                                       <  2:454380/141‑1 (MQ=255)
                                                                               aGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAAAAAAAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCg                                                       <  2:850048/141‑1 (MQ=255)
                                                                                  cAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTg                                                    <  1:70742/141‑1 (MQ=255)
                                                                                   aattaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTgg                                                   <  2:461812/141‑1 (MQ=255)
                                                                                    attaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGc                                                  >  2:368380/1‑141 (MQ=255)
                                                                                    attaattTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGGCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGtgtgc                                                  >  1:328975/1‑137 (MQ=255)
                                                                                      taatttaatAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGcta                                                >  1:285104/1‑141 (MQ=255)
                                                                                        atttaatAATTATAGCAAAATATAGGTTTTGTTGGAAAGTTGAATCTATAGTAAAAAAAAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGctact                                              <  2:421646/141‑1 (MQ=17)
                                                                                                       cagttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                               >  1:24977/9‑141 (MQ=1)
                                                                                                       aagacagttggTTGTCTTGCAAGGTTGAATCTACAACATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAt                               <  1:657601/132‑1 (MQ=11)
                                                                                                         taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                             <  2:536585/135‑1 (MQ=255)
                                                                                                         taaatgaGGTTCTGTTGCAAAGTTGAATTTATAGTAAAAAATAAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTg                             <  2:18048/135‑1 (MQ=11)
                                                                                                         gttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                             >  1:799382/7‑141 (MQ=2)
                                                                                                         aaaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                             <  2:56608/141‑1 (MQ=40)
                                                                                                          ttgggaGGTTTTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                            <  1:744141/136‑1 (MQ=255)
                                                                                                          ttaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGa                            <  2:16319/136‑1 (MQ=9)
                                                                                                           taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                           <  1:377695/137‑1 (MQ=9)
                                                                                                           taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAAAATAAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                           <  2:585949/137‑1 (MQ=11)
                                                                                                           aatgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAg                           >  2:279296/5‑141 (MQ=2)
                                                                                                            tataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGACATACTTTAGATATAAACAATCTATGCACCATTTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGt                          <  1:436212/141‑1 (MQ=14)
                                                                                                            atgaGGTTTTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                          <  2:487902/138‑1 (MQ=255)
                                                                                                            atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                          <  2:725355/138‑1 (MQ=2)
                                                                                                            atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGCAGCTGTTGAGTACGATCTAAGATATGCATTGAGt                          <  1:670465/138‑1 (MQ=11)
                                                                                                             ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATGTGCATTGAGtt                         >  1:472821/1‑141 (MQ=37)
                                                                                                             ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGtt                         >  1:339985/1‑141 (MQ=38)
                                                                                                             ataGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGtt                         <  2:539097/141‑1 (MQ=38)
                                                                                                              gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTa                        <  1:243446/140‑1 (MQ=9)
                                                                                                              gaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAgcta                        <  1:321709/140‑4 (MQ=9)
                                                                                                               aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                       >  2:875207/1‑141 (MQ=0)
                                                                                                               aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                       >  2:696781/1‑141 (MQ=11)
                                                                                                               aGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                       >  2:848128/1‑141 (MQ=11)
                                                                                                               aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTAt                       >  2:113898/1‑141 (MQ=9)
                                                                                                               aGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATAGGCATTGAGTGAt                       >  2:510878/1‑141 (MQ=25)
                                                                                                                     tGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGat                 >  1:689485/1‑141 (MQ=35)
                                                                                                                      tttGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCAATGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGata                <  2:589347/140‑1 (MQ=255)
                                                                                                                       ttGCAAATTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatat               <  2:605130/141‑1 (MQ=21)
                                                                                                                       ttGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatat               <  1:809519/141‑1 (MQ=35)
                                                                                                                       gtggaaaGGTGAATCTATAGAAAAATAAAAAAAAAAAGGAGTCGTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatat               <  2:363652/137‑1 (MQ=11)
                                                                                                                        tGCAAAGTTGAATCCACAGTATAATCTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTATCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGTGTTATCGTGattta              <  2:690263/141‑1 (MQ=11)
                                                                                                                         gCAACGTTGAATCTATAGTATAATTTTAACAAACAGGAATCTTCTGTATGAACTATTTCAGATATAAACAATTTAACACGGATGTTATCACTGTAGCCGTTGGCTACTATCTACGCTATGCATTGAGTTATCGTGgtatat             >  2:151606/1‑141 (MQ=11)
                                                                                                                         gCAAAGTTGAATCTATAGTATAGCATCAACAAAAAGGAGTCTTCTGTATGAGCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatat             >  2:646364/1‑141 (MQ=11)
                                                                                                                         gCAAAGTTGAATCTATAGTATAATTTTAACAACAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTCTCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatat             >  2:15984/1‑141 (MQ=21)
                                                                                                                         gCAAAGTTGAATCTATAGTATAACCACAACAAAAAGGAGTCTTCTGTATGAGCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatatat             >  2:646505/1‑141 (MQ=11)
                                                                                                                          cAAAGTTGAATCTATAATATAATTTTAACAAAGAGGAGTCTGATGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATAACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGctatatg            >  2:264552/1‑140 (MQ=11)
                                                                                                                           aaaGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCt           <  2:285104/141‑1 (MQ=35)
                                                                                                                             aggtGAATTTATAGTATACTTTCAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa         <  1:612438/138‑1 (MQ=11)
                                                                                                                               ttGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaaa       >  2:604061/1‑141 (MQ=34)
                                                                                                                               ttGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaaa       <  2:736047/141‑1 (MQ=35)
                                                                                                                               ttGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaaa       <  2:415728/141‑1 (MQ=35)
                                                                                                                                  aaTCTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAatat    <  1:777396/141‑1 (MQ=21)
                                                                                                                                   aTCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTTTCTAAGATATGCATGGAGTTATCGTGATATATCTGAAATAtt   >  1:212682/1‑141 (MQ=21)
                                                                                                                                    tCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTa  >  1:212555/1‑141 (MQ=34)
                                                                                                                                     |                                                                                                                                           
AATATAACTAATATTGCTGCACCTGTATCACTTAGAACAGCTAACCAAAGTGTTAGTAATCCAGGGAATACTAACACAAAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTA  >  CP000730/76047‑76319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: