Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,850 C→T 39.8% intergenic (+81/+27) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8500CT39.8% 130.8 / 102.0 107intergenic (+81/+27)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (22/42);  new base T (9/33);  total (31/76)
Fisher's exact test for biased strand distribution p-value = 1.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

Only 100 of 107 total aligned reads displayed.

AAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTT  >  CP000730/35719‑35982
                                                                                                                                    |                                                                                                                                    
aaaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAAttt                                                                                                                             >  2:839756/1‑141 (MQ=255)
   gTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATc                                                                                                                          >  1:914776/1‑141 (MQ=255)
       ttAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAATTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGt                                                                                                                      <  1:605838/141‑1 (MQ=255)
         aaTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                                                    <  1:772440/141‑1 (MQ=255)
          aTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAg                                                                                                                   >  1:123511/1‑141 (MQ=255)
           tGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGATAACCACTAATTTATCATGTCAgt                                                                                                                  >  2:152874/1‑141 (MQ=255)
            gTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAgtg                                                                                                                 <  1:124521/141‑1 (MQ=255)
                 aGCTATTCAGTATAAAGTTTTTGGGCGAAAAGGAGGTCCTGCCCCGAAATTAAAAGAAAATTGTGATCTAATAAAAATAGAATGTTCTGTTGCAAAGTAAAAAAATATAGCTACCCACTAATTTATCATGTCAGTGTTCGc                                                                                                            <  1:206360/141‑1 (MQ=12)
                 aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                            >  2:77941/1‑141 (MQ=255)
                 aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                            <  1:749364/141‑1 (MQ=255)
                 aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                            <  1:22530/141‑1 (MQ=255)
                   cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAAGATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtt                                                                                                          <  1:626844/141‑1 (MQ=255)
                       tCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACt                                                                                                      <  1:8187/141‑1 (MQ=255)
                        cAGTATAAAGTTTTTGGCGAAAAATGATTACGTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                                                     <  2:279361/141‑1 (MQ=255)
                          gTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                                                   >  2:757740/1‑141 (MQ=255)
                           tataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                                                  <  1:775893/141‑1 (MQ=255)
                           tataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCt                                                                                                  >  2:67857/1‑141 (MQ=255)
                            ataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTa                                                                                                 >  1:549543/1‑141 (MQ=255)
                             taAAGTTTCTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATAGAAAAAAAAAAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAg                                                                                                <  2:841499/141‑1 (MQ=21)
                             aaatggctcaggggAAAAAGGAGAACTTGACAAAAAGCTAAAATAAAATTGTGACCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAg                                                                                                <  2:274424/128‑1 (MQ=18)
                              aaaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTATCCACTAATTTATCATGTCAGTGTTAGCTTAACTTGCTCGc                                                                                               >  2:464259/1‑141 (MQ=255)
                              aaaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGc                                                                                               >  2:569819/1‑141 (MQ=255)
                              aaaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTGTCTAGc                                                                                               >  2:377566/1‑141 (MQ=255)
                                    tttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                                                         >  2:30806/1‑141 (MQ=255)
                                      tGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAATAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCACGATGCt                                                                                       <  2:501501/141‑1 (MQ=255)
                                       ggagAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTa                                                                                      <  2:52091/138‑1 (MQ=255)
                                         cGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAAt                                                                                    <  2:148211/141‑1 (MQ=255)
                                          gAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAAtt                                                                                   >  2:740145/1‑141 (MQ=255)
                                           aaaaaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAAttt                                                                                  >  1:860225/1‑141 (MQ=255)
                                             aaaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCg                                                                                <  1:198677/141‑1 (MQ=255)
                                                    tACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTAGCAAAATAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATg                                                                         <  2:632186/141‑1 (MQ=255)
                                                     aCTTGAACAAAAGCTAAAATAAAATTGTGATCTAATAAAAATAGAGGGTTCTGTTGCAAAGTAAAAAAATATAGCTCACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATgg                                                                        <  2:42846/141‑1 (MQ=255)
                                                      cTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                                                       <  1:89342/141‑1 (MQ=255)
                                                        tGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGAATgggtg                                                                     <  2:689939/141‑4 (MQ=255)
                                                         gAACGAGGGCTAAAATAAAGGTGCGATCGAATGAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTCTCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaa                                                                    <  1:410766/141‑1 (MQ=14)
                                                         gAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaa                                                                    >  2:475127/1‑141 (MQ=255)
                                                         gAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaa                                                                    <  1:241334/141‑1 (MQ=255)
                                                            cAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAt                                                                 >  2:26670/1‑141 (MQ=255)
                                                            cAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAt                                                                 <  1:839756/141‑1 (MQ=255)
                                                                     aaataaaatTGTGATCTAATAAAAATAGAAGGTTATGTTGCAAAGTAAAGAAATATAGCTAACCACTAATTTATCAAGTCAGTCTTCGCTTAACTTGCGAGCATGATGCTAATTTCGTGGCATGGAGAAAAACCGtgagat                                                        >  2:833013/1‑136 (MQ=17)
                                                                       ataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTg                                                      >  1:692858/1‑141 (MQ=255)
                                                                       acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                    >  1:372424/15‑141 (MQ=11)
                                                                       acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGaa                                                    >  1:637907/15‑141 (MQ=11)
                                                                         aaaagattattttaataaAAATGATGGTGCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                                                  <  2:820845/129‑1 (MQ=11)
                                                                            agagtattttaataaAAATGATTGTTTTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                               <  2:250415/132‑1 (MQ=11)
                                                                            agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                               <  1:711371/132‑1 (MQ=11)
                                                                            agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                               <  1:255959/132‑1 (MQ=11)
                                                                            agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                               <  2:725066/132‑1 (MQ=11)
                                                                            agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                               <  1:731387/132‑1 (MQ=11)
                                                                            agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCCAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                               <  1:360810/132‑1 (MQ=11)
                                                                            agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCGAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                               <  1:703686/132‑1 (MQ=11)
                                                                            agagaattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                               <  2:250427/132‑1 (MQ=11)
                                                                            agaaaaattaaataaAAATGATGGTTCGCTTGCAAAGTAAGAAAAATAGCTAAGCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCAAAAAAAAAAAAAAATCCGTAGATCTGAAGAGAc                                               <  2:894634/131‑1 (MQ=2)
                                                                             gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTGTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                              <  2:549398/133‑1 (MQ=11)
                                                                             gagtattttaataaAAATGATGGTTCTGTTGCAAAGGAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                              <  1:86609/133‑1 (MQ=11)
                                                                              tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                               <  1:880498/141‑1 (MQ=255)
                                                                              tgtgATCCAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                               <  2:115639/141‑1 (MQ=255)
                                                                              ggtattttaataaAAATGATGGTTCTGTTTCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                             <  2:384849/134‑1 (MQ=11)
                                                                              agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                             <  2:685904/134‑1 (MQ=11)
                                                                              agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                             <  1:15172/134‑1 (MQ=11)
                                                                               gtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAACTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                              <  1:382603/141‑1 (MQ=255)
                                                                               gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGAGAAAACCCGTATATCTTAAGAGACCTg                                            <  2:307649/135‑1 (MQ=11)
                                                                               gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAACTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                            <  2:231585/135‑1 (MQ=11)
                                                                                tgATATAATAAAAATAGAAGGTTCGGTTGCAAAGTAAAAAAATATAGCTAACCACTAATGTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                             <  1:748040/141‑1 (MQ=255)
                                                                                tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                           <  2:846868/136‑1 (MQ=11)
                                                                                tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                           <  1:520612/136‑1 (MQ=11)
                                                                                tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATACCTACTCCCTAATATATCATGTCAGTGTTCGTTTAACTTGCTTGCTCTATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                           <  1:759834/136‑1 (MQ=11)
                                                                                tattttaataaAAATGATGGTTATGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                           <  1:542973/136‑1 (MQ=11)
                                                                                tattttaataaAAATGATGGTCCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAAGTTGCTAGCATGATGCTAATTTCGTGGCATGCAGAAAATCCGTTGATCTGGAGAGACCTGc                                           >  1:467471/6‑141 (MQ=11)
                                                                                tattttaataaAAATGATAGTTCTTTTGCAAAGTAAAATATATAGCTAATCACTAATATATCATGGCAGTGTTCGTTTAACTTGCTAGCATGCTGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                           <  2:270311/136‑1 (MQ=11)
                                                                                 gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                            <  1:324806/141‑1 (MQ=255)
                                                                                 gATCCAATAAAAAGAGAAGGTTCTGCTGCAAAGTAAAAAAATATAGCTACCCACCAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                            <  1:111022/141‑1 (MQ=14)
                                                                                 attttaataaAAATGATGGTTCTGTTGCAACGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATAATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGGGACCTGCg                                          <  2:670742/137‑1 (MQ=11)
                                                                                 attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGGAGATCTGAAGAGACCTGCg                                          <  1:907628/137‑1 (MQ=11)
                                                                                 attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAACCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                          <  1:303752/137‑1 (MQ=11)
                                                                                  ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                         >  1:747213/4‑141 (MQ=11)
                                                                                  ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                         >  1:843767/4‑141 (MQ=11)
                                                                                  ttttaataaAAATGATGGTTCTGTGGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                         >  1:280754/4‑141 (MQ=11)
                                                                                  aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                           <  1:16977/141‑1 (MQ=255)
                                                                                  aTCAAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACGAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                           <  2:202153/141‑1 (MQ=255)
                                                                                   tttaataaAAATGATGGTTCTGTTGGAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                        <  2:715579/139‑1 (MQ=11)
                                                                                   tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                        <  2:530510/139‑1 (MQ=11)
                                                                                   tCTAATAAAGATAGAAGTTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                          <  2:798581/141‑1 (MQ=255)
                                                                                   tCTAATAAAAATAGAAGGTTCTGTTGCAAAGGAAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                           <  1:476349/141‑1 (MQ=255)
                                                                                    ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                       <  2:516120/140‑1 (MQ=11)
                                                                                    cTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACTACTATTTTATCTTGTCAGTGTTCGCTTAACTTGCTTGTATGATGCTGATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                         <  1:192424/141‑1 (MQ=14)
                                                                                    cTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATTATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                         <  1:77604/141‑1 (MQ=255)
                                                                                    ataataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTCGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                         <  2:787741/140‑1 (MQ=255)
                                                                                     taataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                        <  1:820481/141‑1 (MQ=40)
                                                                                      aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                     >  1:834674/1‑141 (MQ=11)
                                                                                       ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                                    >  1:325594/1‑141 (MQ=11)
                                                                                       ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGGTCtt                                    >  1:340235/1‑141 (MQ=11)
                                                                                        taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                   <  1:438144/141‑1 (MQ=11)
                                                                                        taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                   <  2:113761/141‑1 (MQ=11)
                                                                                             aTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTta                                <  2:447967/141‑1 (MQ=33)
                                                                                               ggctgggtCTGTTGCAAAGCAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGATCTACGGTTCTTTTtatat                             <  1:181338/134‑1 (MQ=1)
                                                                                               aGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTCATTTATCATGACAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGCTCTATGtatg                              >  1:111532/1‑140 (MQ=11)
                                                                                                gAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAAATCTGAAGAGACCTGCGGATCTTTTtatct                             >  2:204979/1‑139 (MQ=9)
                                                                                                  ggggtcggTTGCAAAGTAAAAAATATAGCTAGTGACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATaga                          <  2:856567/134‑1 (MQ=1)
                                                                                                                            ttatcTAACCACTAAGTTAACAGGTCAGTGTTCGGTTAACGTTCTAGCATTATGCTAATTTCGTTGTATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTATTTTTATATAGAGCGTAAATACATTCAATACCtttt  <  1:187955/137‑1 (MQ=1)
                                                                                                                                    |                                                                                                                                    
AAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTT  >  CP000730/35719‑35982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: