BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	201,369	A→G	3.7%	T292A (ACG→GCG)	fdh →	formate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	201,369	0	A	G	3.7%	311.1 / 2.2	109	T292A (ACG→GCG)	fdh	formate dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (45/59); new base G (2/2); total (47/62)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.27e-01

Only 100 of 112 total aligned reads displayed.

CACGTGGTAAAATTGTAAATCGCGATGCGTTAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTCGTATTGCTAGTAAA  >  CP000730/201229‑201509

cACGTGGTAAAATTGTAAATCGCGATGCGGATATAGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGa                                                                                                                                              >  2:378153/1‑141 (MQ=255)

  cGTGGTAAAATTGTAAATCGCGATGCGTTAGTTGAAGCGTTAGCATCGGAGCATTTAAAAGGATATGCTGGTGCCGCTTGGTATATACAAGCTGCACCGGCGGACCACCCACGGAGAACAATGCCGAGAAATGCTATGACg                                                                                                                                            >  2:304285/1‑141 (MQ=255)

   gTGGTAAAATTGTAAATCGCGATGCGTTAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGGACAATGCCTAGAAATGCTATGACgg                                                                                                                                           >  2:479888/1‑141 (MQ=255)

     tgTAAAATTGTAAATCCCGATGCGTTAGTTGAAGCGTTAGCACCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGtt                                                                                                                                         <  1:169553/140‑1 (MQ=255)

     ggTAAAATTGTAAATCGCGATGCGTTAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCCCCTACTGAGCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGtt                                                                                                                                         >  1:167748/1‑141 (MQ=255)

      gTAAAATTGTAAATCGCGATGCGTTAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTc                                                                                                                                        <  1:305708/141‑1 (MQ=255)

         aaaTTGTAAATCGCGATGCGTTAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACt                                                                                                                                     <  2:709739/141‑1 (MQ=255)

         aaaCTGTAAATCGCGATGCGTCAATTGAAGCGCTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACt                                                                                                                                     <  1:69586/141‑1 (MQ=255)

                aaaTCGCGATGCGTTAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATTGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAgg                                                                                                                              <  1:104393/141‑1 (MQ=255)

                aaaTCGCGATGCGTTAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGGCGGTTCACTATTCAgg                                                                                                                              >  2:480521/1‑141 (MQ=255)

                       gATTCGTTAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACt                                                                                                                       <  1:332599/141‑1 (MQ=255)

                       gATTCGTTAGTGGAAGCGTTAGCATCCGAGCATTAACAAGGATATGCTTGAGATGTTTGGTAGCCACAACCTGCACCTGCTGATCATCCATGGCGAACAATGCCTAGAAAGGCTATGACGGTTCACTATTCAGGTATGACt                                                                                                                       <  1:548587/141‑1 (MQ=255)

                        aTGCGTTAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACtt                                                                                                                      >  1:782550/1‑141 (MQ=255)

                        aTGCGTTAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACtt                                                                                                                      >  1:193734/1‑141 (MQ=255)

                           cGTTAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAg                                                                                                                   >  1:779545/1‑141 (MQ=255)

                           cGTTAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGAGATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAg                                                                                                                   >  1:585537/1‑141 (MQ=255)

                            gTTAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAAGGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGa                                                                                                                  >  1:618035/1‑141 (MQ=255)

                             ttAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGaa                                                                                                                 <  1:405837/141‑1 (MQ=255)

                               aGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGc                                                                                                               <  2:767036/141‑1 (MQ=255)

                                  tGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGcaca                                                                                                            <  2:221506/141‑1 (MQ=255)

                                  tGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGcaca                                                                                                            <  2:578002/141‑1 (MQ=255)

                                       cGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAAc                                                                                                       >  2:327833/1‑141 (MQ=255)

                                        gTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACg                                                                                                      <  1:327833/141‑1 (MQ=255)

                                        gTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCAACAACCTGCACCTGCTGATCATCCATGGAGAACACTGCATGGGAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACg                                                                                                      >  1:569811/1‑141 (MQ=255)

                                        gTTAGCATCCGAGCAATTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACg                                                                                                      >  1:674368/1‑141 (MQ=255)

                                         ttAGCATCCGAGCATCTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGt                                                                                                     <  2:825079/141‑1 (MQ=255)

                                          tAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGCAGCACAAAAACGTa                                                                                                    <  2:331584/141‑1 (MQ=255)

                                          tAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTCATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTa                                                                                                    >  2:549306/1‑141 (MQ=255)

                                            gCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTAtt                                                                                                  >  1:137557/1‑141 (MQ=255)

                                             cATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTg                                                                                                 <  1:5906/141‑1 (MQ=255)

                                               tCCGAGCATTTACAAGGATTTGCCGGTGGTGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGaa                                                                                               <  2:896654/141‑1 (MQ=255)

                                               tCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGaa                                                                                               <  2:451694/141‑1 (MQ=255)

                                                ccGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAg                                                                                              <  2:425132/141‑1 (MQ=255)

                                                   aGCATTTACAAGTATCGGCTGGTGTTGTTTGGTATCCACCACCGGCGCCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATg                                                                                           <  1:616423/141‑1 (MQ=255)

                                                    gCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATgg                                                                                          >  2:202116/1‑141 (MQ=255)

                                                     cATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGa                                                                                         <  1:643925/141‑1 (MQ=255)

                                                      aTTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAg                                                                                        <  1:723388/141‑1 (MQ=255)

                                                           cAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTaaa                                                                                   >  2:695971/1‑141 (MQ=255)

                                                              ggATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGat                                                                                >  1:858722/1‑141 (MQ=255)

                                                               gATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGata                                                                               >  1:143453/1‑141 (MQ=255)

                                                                   tGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATGATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATTACTTTACAAGCGCAACAACGAAAAGAAGATGGAGTTAAAGGTAtgtt                                                                           >  2:592767/1‑141 (MQ=255)

                                                                   tGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATAtttt                                                                           >  2:596872/1‑141 (MQ=255)

                                                                    gCTGGTGCTGATTGGTCTCCACCACCTGCACTTGCTGATCATCAATGGAGAACCATGCCTAGAAATGCTATGACGGTTCACTATTAAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGCGTTGAAGatagtcat                                                                          >  2:291096/1‑136 (MQ=255)

                                                                    gCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTa                                                                          <  2:415690/141‑1 (MQ=255)

                                                                     cTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTag                                                                         >  1:889439/1‑141 (MQ=255)

                                                                        gTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAGTGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATTGAGTTAAAGATATTTTAGAGc                                                                      <  1:468158/141‑1 (MQ=255)

                                                                         tGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCg                                                                     <  1:509328/141‑1 (MQ=255)

                                                                          gATGTTGGGTATCCACAACCTGCACCTGCTGATCATGAATGGAGAAGAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGt                                                                    <  1:390828/141‑1 (MQ=255)

                                                                           aTGTTTGGTATCCACAACCTGCACCTGCTGATCAACCATGTAGAATAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGtt                                                                   <  1:702253/141‑1 (MQ=255)

                                                                             gTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTttc                                                                 >  2:248409/1‑141 (MQ=255)

                                                                              tttGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTttct                                                                <  1:329446/141‑1 (MQ=255)

                                                                              tttGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTGTAGAGCGTttct                                                                <  1:261785/141‑1 (MQ=255)

                                                                                tGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTttcttc                                                              >  2:40542/1‑141 (MQ=255)

                                                                                 ggTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCa                                                             >  1:789827/1‑141 (MQ=255)

                                                                                  gTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGGTAAAGATATTTTAGAGCGTTTCTTCaa                                                            >  1:316085/1‑141 (MQ=255)

                                                                                   tATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAACCGTATTAAAGATGGAGTTAAAGATATGGTAGAGCGTTTCTTCAAt                                                           >  1:734493/1‑141 (MQ=255)

                                                                                   tATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATGGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAAt                                                           >  1:102138/1‑141 (MQ=255)

                                                                                      ccACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCAt                                                        >  1:434468/1‑141 (MQ=255)

                                                                                      ccACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCAt                                                        >  1:434484/1‑141 (MQ=255)

                                                                                      ccACAACCTGCACCAGCTGATCATCCATGAAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCAt                                                        >  1:486525/1‑141 (MQ=255)

                                                                                       cacaACCTGCCCCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATg                                                       <  2:459337/141‑1 (MQ=255)

                                                                                       cacaACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATg                                                       <  2:736386/141‑1 (MQ=255)

                                                                                           acctgcacctgcTGATNATTNANGNANAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAAcc                                                   <  1:583189/141‑1 (MQ=255)

                                                                                           acctgcacctgcTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGATCAAGTTTTAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCCATCATGAAcc                                                   <  1:662390/141‑1 (MQ=255)

                                                                                              tgcacctgcTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCttt                                                >  2:113738/1‑141 (MQ=255)

                                                                                              tgcacctgcTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCttt                                                >  1:566819/1‑141 (MQ=255)

                                                                                                cacccgcTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTcc                                              <  1:304285/141‑1 (MQ=255)

                                                                                                  cctgcTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTGCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCaa                                            <  1:888214/141‑1 (MQ=255)

                                                                                                  ccggctgATGAACAATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCGGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGCAGTTCACGATAGTTTAGTACGTGTCTTCGATCATGAACCTTTACaa                                            >  2:532775/4‑141 (MQ=255)

                                                                                                    tgctgATCATCCATGGAGAACAATGCCTAGACACGCAATGACGGCTCAGTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCaaga                                          <  2:189472/141‑1 (MQ=255)

                                                                                                     gctgATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCaagat                                         <  1:542883/141‑1 (MQ=255)

                                                                                                      ctgATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCaagata                                        >  1:342968/1‑141 (MQ=255)

                                                                                                           catcCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGTTGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCaagataaagat                                   <  1:549306/141‑1 (MQ=255)

                                                                                                             tcCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGatat                                 <  1:713752/141‑1 (MQ=255)

                                                                                                              ccATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGAtatt                                >  2:703340/1‑141 (MQ=255)

                                                                                                              ccATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGAtatt                                >  2:769397/1‑141 (MQ=255)

                                                                                                                 cggcgaACAATTCCTAGACCAGATACGAAGGATCATTTTCCAGATATGACTTTCGAAGCACAAAAACGTATTGCAGATAGAGATAAAGAGCTGTTAGAGCAATTCTTCAATCATGAACCTTTCCAAGATAAAGATctaatt                             >  2:418670/5‑141 (MQ=255)

                                                                                                                  ggAGAACAATGCCTAGAAATGCTATGGCGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAACTAAGAGTCGTGCCAAGATAAAGATATTAttg                            <  1:440127/141‑1 (MQ=255)

                                                                                                                      aaCAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACATAAAAAAAAAAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGc                        <  2:113044/141‑1 (MQ=255)

                                                                                                                        cAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGATGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTCAGAGCGTTTCTTCAATCAGGAATCTTTCCAAGATAAGGATATTATTGTTGCaa                      <  2:553490/141‑1 (MQ=255)

                                                                                                                        cAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCaa                      <  1:612988/141‑1 (MQ=255)

                                                                                                                         aaTGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAg                     >  2:479199/1‑141 (MQ=255)

                                                                                                                           tGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTg                   >  1:6471/1‑141 (MQ=255)

                                                                                                                           agCCTAGAAACGCTATGACGGTGCACTATTCAGGTATGACTGTAGAAGCACAAAAACGTATGGAACATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTGTCCAAGATAAAGATATTATTGTTGCAAGTg                   <  2:758951/140‑1 (MQ=255)

                                                                                                                            gCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTgg                  <  2:585537/141‑1 (MQ=255)

                                                                                                                            gCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTgg                  <  2:779545/141‑1 (MQ=255)

                                                                                                                              cTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTc                <  1:780275/141‑1 (MQ=255)

                                                                                                                               tAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTCg               <  1:378153/141‑1 (MQ=255)

                                                                                                                               tAGAAATGCTATGACGGTTAACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTCg               <  1:769947/141‑1 (MQ=255)

                                                                                                                                   aaggCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTCGTAtt           <  2:472054/138‑1 (MQ=255)

                                                                                                                                   aaTGCTATGACGGTTCACTATTCAGTTATGACTTTAGAAGCATAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTCTTAtt           <  2:788718/141‑1 (MQ=255)

                                                                                                                                      gCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTCGTATTGCt        >  1:785690/1‑141 (MQ=255)

                                                                                                                                       cTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTCGTATTGCTa       <  1:479888/141‑1 (MQ=255)

                                                                                                                                         tggACGGTTCACTAGTCAGGTATGTCTTTAGAAGCACAAAATCGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTCGTATTGCTAGt     <  2:618035/139‑1 (MQ=255)

                                                                                                                                         acgACGGTTCACTGTTCCGGTATGCCTGTAGAAGCACAAAAGCGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTCGTATTGCTAGt     <  1:685425/139‑1 (MQ=255)

                                                                                                                                         aTGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATGTTATTGTTGCAAGTGGTCGTATTGCTAGt     >  1:205314/1‑141 (MQ=255)

                                                                                                                                         aTGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATCAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTCGTATTGCTAGt     <  1:602845/141‑1 (MQ=255)

                                                                                                                                           gACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTCGTATTGCTAGTaa   >  2:250869/1‑141 (MQ=255)

                                                                                                                                            aCGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTCGTATTGCTAGTaaa  >  1:523180/1‑141 (MQ=255)

                                                                                                                                            aCGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTCGTATTGCTAGTaaa  >  1:14308/1‑141 (MQ=255)

CACGTGGTAAAATTGTAAATCGCGATGCGTTAGTTGAAGCGTTAGCATCCGAGCATTTACAAGGATATGCTGGTGATGTTTGGTATCCACAACCTGCACCTGCTGATCATCCATGGAGAACAATGCCTAGAAATGCTATGACGGTTCACTATTCAGGTATGACTTTAGAAGCACAAAAACGTATTGAAGATGGAGTTAAAGATATTTTAGAGCGTTTCTTCAATCATGAACCTTTCCAAGATAAAGATATTATTGTTGCAAGTGGTCGTATTGCTAGTAAA  >  CP000730/201229‑201509

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`