Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 293,940 C→T 19.6% intergenic (+57/‑471) USA300HOU_0261 → / → ispD2 zinc (Zn2+)‑binding dehydrogenase/2‑C‑methyl‑D‑erythritol 4‑phosphate cytidylyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730293,9400CT19.6% 136.4 / 30.2 73intergenic (+57/‑471)USA300HOU_0261/ispD2zinc (Zn2+)‑binding dehydrogenase/2‑C‑methyl‑D‑erythritol 4‑phosphate cytidylyltransferase
Reads supporting (aligned to +/- strand):  ref base C (32/25);  new base T (7/7);  total (41/32)
Fisher's exact test for biased strand distribution p-value = 7.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-02

CCGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTGATAA  >  CP000730/293800‑294080
                                                                                                                                            |                                                                                                                                            
ccGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGc                                                                                                                                              <  1:694294/141‑1 (MQ=255)
ccGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGc                                                                                                                                              <  2:808773/141‑1 (MQ=255)
  gTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGCACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCg                                                                                                                                            <  1:806144/141‑1 (MQ=255)
  gTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCg                                                                                                                                            <  2:295822/141‑1 (MQ=255)
     aGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCt                                                                                                                                         <  2:847103/141‑1 (MQ=255)
      gAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTg                                                                                                                                        <  2:708056/141‑1 (MQ=255)
          aaGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCt                                                                                                                                    <  1:279417/141‑1 (MQ=255)
           aGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGAGTTCTTAGTATGCACCTTTGAGAACGAAAACGCTGGTGTAGTTATAGCTATGAAACGGCGAATGACGTCTGGtata                                                                                                                                   >  2:243664/1‑141 (MQ=255)
            ggCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCtat                                                                                                                                  <  2:13603/141‑1 (MQ=255)
              cccGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATACCGGCGCGCTGGCGGTTTATACCAGTTCAGCTATGAGGACGAAAGCGAGGCGATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAg                                                                                                                                >  1:901640/1‑141 (MQ=255)
              cccGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAg                                                                                                                                >  1:789951/1‑141 (MQ=255)
              cccGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAg                                                                                                                                >  1:713489/1‑141 (MQ=255)
                  gagaCATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATAc                                                                                                                            >  2:872379/1‑141 (MQ=255)
                  gagaCATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGATGGTTTTGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATAc                                                                                                                            <  1:479176/141‑1 (MQ=255)
                   agaCATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTGTGAAAGTGCGAATGCCGGCTGGTCTATAGATACt                                                                                                                           >  2:239430/1‑141 (MQ=255)
                   agaCATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACt                                                                                                                           >  1:179741/1‑141 (MQ=255)
                      cATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATc                                                                                                                        >  2:536225/1‑141 (MQ=255)
                       atgtGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGCTCTATAGATACTATCg                                                                                                                       <  1:505820/138‑1 (MQ=255)
                         ttGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGaa                                                                                                                     >  1:458081/1‑141 (MQ=255)
                            aTAAATTAGGTAACAAGAAAGAACGATTTGATAAAGTCATGTTGACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAaata                                                                                                                  <  1:682169/141‑1 (MQ=255)
                                 ttAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCa                                                                                                             >  1:597583/1‑141 (MQ=255)
                                     ttAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCtt                                                                                                         <  1:534480/141‑1 (MQ=255)
                                            gaaagaaCGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCGCCTTTGAGGAAGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGACGTCTGGTCTATAGATACTATCGAAATAATTCATGTTCGCatat                                                                                                  >  2:149543/1‑141 (MQ=255)
                                            gaaagaaCGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAatat                                                                                                  >  1:16399/1‑141 (MQ=255)
                                            gaaagaaCGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAatat                                                                                                  >  1:4200/1‑141 (MQ=255)
                                            gaaagaaCGATTTGATAAAGTCATATTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAatat                                                                                                  >  1:664348/1‑141 (MQ=255)
                                                gaaCGCTTTGATAAAGTCATGTTCACGCTATATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGt                                                                                              >  2:881483/1‑141 (MQ=255)
                                                 aaCGATTAGATAAAGTGATGTTTACGATGTATTAGTAAGCACCTTTGAGGACGAAAACGCTGGTATAGGGATAGCGATGAAAGTGCGAATGTCGGCGAGTCTATAGATACTATCGAAATAATGCAACTTCGAatacaggct                                                                                             >  2:278005/1‑135 (MQ=255)
                                                    gATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGAt                                                                                          >  1:69316/1‑141 (MQ=255)
                                                     aTTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATa                                                                                         >  2:657372/1‑141 (MQ=255)
                                                            aaaGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGcc                                                                                  <  1:331743/141‑1 (MQ=255)
                                                              aGTCATGTTTACGATTTATTAGTGTGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCgg                                                                                <  1:78925/141‑1 (MQ=255)
                                                              aGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCgg                                                                                <  1:681234/141‑1 (MQ=255)
                                                                ctaagTTTACGATTAATTAGGATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGTTATGAAAGTGCGAATGCCGTCGGGTCTATAGATACGATCGAATGTATTCATCTTCGAATATACGTTGATAAATATCCGGtt                                                                              <  2:398878/137‑1 (MQ=255)
                                                                 cATGTTGACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTAAAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGttt                                                                             >  2:572725/1‑141 (MQ=255)
                                                                   tGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTAc                                                                           <  2:659993/141‑1 (MQ=255)
                                                                     tttAAGATATAGTAGTATGCACCTTTGAGGACGAAAACGATGGTATAGTTATAGCTATGAAAGTGCGACTGCCGTGTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGGTTAAtt                                                                         >  2:334390/1‑141 (MQ=255)
                                                                      ttACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGATCTATAGATACTTTCGAAACACTTCATCTTCGAATATACGTTGCTAAATAGCCGGTTTACttt                                                                        >  1:186305/1‑140 (MQ=255)
                                                                        aCGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAATTAGCGGGTGTACTtgtg                                                                      >  2:480696/1‑141 (MQ=255)
                                                                        aCGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTtgtg                                                                      <  2:61604/141‑1 (MQ=255)
                                                                        aCGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTggtg                                                                      >  2:566620/1‑141 (MQ=255)
                                                                              tattaGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAat                                                                >  2:608159/1‑141 (MQ=255)
                                                                                   gTATCCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTCATAAATAGCCGGTTTACTTGTGTGAAATATACt                                                           >  1:618334/1‑141 (MQ=255)
                                                                                         aCCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGTAATATGCTTGTGaa                                                     <  1:594581/141‑1 (MQ=255)
                                                                                              tGAGGACGAAAACGCTGGTATAGTTATAGCTATGACAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATGTACGTGGATAAATAGCCGCGTTACTTTAGTGTCATTCGCCTGTGATTCggg                                                >  1:16376/1‑140 (MQ=255)
                                                                                              tGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgt                                                <  1:761522/141‑1 (MQ=255)
                                                                                              tGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTTTGAAATATTCTTGTGAATCGgt                                                >  1:150645/1‑141 (MQ=255)
                                                                                              tGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgt                                                >  1:838279/1‑141 (MQ=255)
                                                                                              tGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgt                                                <  1:441554/141‑1 (MQ=255)
                                                                                                 ggagggaaaCGCTGGTATAGTTAGAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTCCTTGTGTGAAATATGCTTGTGAATGGgttgt                                             >  2:906492/7‑141 (MQ=255)
                                                                                                 ggACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgttgt                                             >  2:764693/1‑141 (MQ=255)
                                                                                                 ggACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGgttgt                                             <  1:872379/141‑1 (MQ=255)
                                                                                                    cGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGtttt                                          <  2:627249/141‑1 (MQ=255)
                                                                                                        aaCGCCGGTATAGTTATAGCAATGAAAGTGCGAATACCGTCTGGTCAATAAATACAATCGAAAACATTCATCTACGAATCTCCGTTGATAAATAGCTGGTTTACTTGTGTGAAATATGCTTGTGACTCGGTTGTTTTGCAt                                      >  2:28013/1‑141 (MQ=255)
                                                                                                              ggtataggtataGCTATGAAGGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGta                                >  1:568676/1‑141 (MQ=255)
                                                                                                               gTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATACTTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATAGGTTGTTTTGCATTTTGtat                               <  1:572725/141‑1 (MQ=255)
                                                                                                                 ataGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATAc                             >  1:21530/1‑141 (MQ=255)
                                                                                                                 ataGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATAc                             >  1:327458/1‑141 (MQ=255)
                                                                                                                 ataGTTATAGCTAAGAAAGTGCAAATTCCGACTGTTCCATAGATACTATCGAAATAATTCATCCTCGAATATACGTTGATAAATAGGCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATAc                             <  2:118859/141‑1 (MQ=255)
                                                                                                                  taGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTTTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACt                            <  1:239430/141‑1 (MQ=255)
                                                                                                                  taGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACt                            <  2:179741/141‑1 (MQ=255)
                                                                                                                       ataGCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTaaaa                       <  1:480696/141‑1 (MQ=255)
                                                                                                                       aaaGCTATGAAAGTGAGAATGCCGACTGGTCTATAGATACTATCGGAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTcaaa                       <  2:155162/139‑1 (MQ=255)
                                                                                                                        taGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAAt                      >  1:76904/1‑141 (MQ=255)
                                                                                                                         aGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATg                     <  2:870862/141‑1 (MQ=255)
                                                                                                                         aGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATg                     <  2:774285/141‑1 (MQ=255)
                                                                                                                          gCTATGAAAGTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATga                    >  1:711920/1‑141 (MQ=255)
                                                                                                                             aTGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGAt                 >  1:628779/1‑141 (MQ=255)
                                                                                                                              tGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTATAATGAGATg                <  1:818369/141‑1 (MQ=255)
                                                                                                                              tGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATg                <  2:359140/141‑1 (MQ=255)
                                                                                                                                   gTGCGAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAat           >  2:738791/1‑141 (MQ=255)
                                                                                                                                   gTGCGAATGCCGTCAGGTCTATCGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGCTGTTTTGCATTTTGTATACTTAAAATGAGATGGCaaa           >  2:26662/1‑140 (MQ=255)
                                                                                                                                       gAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATCTCCTCGCGTGGCGGTGGGTTTGCATTTTGTATACTTAAAATGAGATGGCAATAttt       >  1:428597/1‑141 (MQ=255)
                                                                                                                                       gAATGTCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATAttt       <  1:373127/141‑1 (MQ=255)
                                                                                                                                        aaTGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCGTCGAATATACGTTGATAAATAGCCGGTTTACTTGAGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTAAAAATGAGATGGCAATAttgg      >  2:611235/1‑139 (MQ=255)
                                                                                                                                            ccGGCTGGTCTATAGATACTATCGGAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTTTCCTTAAAATGAGATGGCACTGTTTGAGaa  >  2:532847/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CCGTTAGAAAAAGGCCCGGAGACATTTGATAAATTAGTTAACAAGAAAGAACGATTTGATAAAGTCATGTTTACGATTTATTAGTATGCACCTTTGAGGACGAAAACGCTGGTATAGTTATAGCTATGAAAGTGCGAATGCCGTCTGGTCTATAGATACTATCGAAATAATTCATCTTCGAATATACGTTGATAAATAGCCGGTTTACTTGTGTGAAATATGCTTGTGAATCGGTTGTTTTGCATTTTGTATACTTAAAATGAGATGGCAATATTTGATAA  >  CP000730/293800‑294080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: