BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	317,291	A→G	5.2%	M192T (ATG→ACG)	USA300HOU_0283 ←	ribose operon repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	317,291	0	A	G	5.2%	317.8 / 4.6	124	M192T (ATG→ACG)	USA300HOU_0283	ribose operon repressor
Reads supporting (aligned to +/- strand): ref base A (55/59); new base G (4/3); total (61/63)
Fisher's exact test for biased strand distribution p-value = 7.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.76e-01

Only 100 of 131 total aligned reads displayed.

AGATTGCAGTGGCAGATTGAACCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCTGTATAGCCTTGATGGTCATTTTGATCC  >  CP000730/317151‑317431

aGATTTCAGTGGCAGATTGAACCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACa                                                                                                                                              <  1:466156/141‑1 (MQ=255)

aGATTGCGGTGGCAGATGTAACTATGACGTCATCAACAATGGTTAGCCCATCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACa                                                                                                                                              <  1:840966/141‑1 (MQ=255)

  aTTGCAGTGGCAGATTGAACCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACata                                                                                                                                            >  2:183741/1‑141 (MQ=255)

   ttGCAGTGGCAGATTGAACCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACatat                                                                                                                                           <  1:879702/141‑1 (MQ=255)

   ttGCAGTGGCAGATTGAACCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACatat                                                                                                                                           >  1:797826/1‑141 (MQ=255)

     gCAGTGGCAGATTGAACCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTGAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTc                                                                                                                                         >  2:673937/1‑141 (MQ=255)

      cAGTGGCCGATTGAACCATGATGTCATCAACAATGGTTACCCCACCGCGCTCAGATAATTCAGTATGGACGATTTGTGGTTCTGTCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCg                                                                                                                                        <  1:266175/141‑1 (MQ=255)

      cAGTGGCAGATTGAACCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCg                                                                                                                                        <  2:127994/141‑1 (MQ=255)

       ggTGGCAGATGGAACCATGATGTCATCAACAATGGTTAGCCCCCCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGc                                                                                                                                       <  2:808617/140‑1 (MQ=255)

       aGTGGCAGATTGAACCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGc                                                                                                                                       >  1:568490/1‑141 (MQ=255)

       aGTGGCAGATTGAACCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGc                                                                                                                                       <  2:702644/141‑1 (MQ=255)

       aGTGGCAGATTGAACCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATGCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGCCACGAGTCGCCATATTCGc                                                                                                                                       >  2:114870/1‑141 (MQ=255)

          ggCAGATTGAACCATGATGTCAACACCAGAGAATAGGCCACCGCGCTTAGATAATTCAGAATGGACGATTTGTGGTTCTGGCAATTGATTCACGCGCAAAGTATCGACAAATCAAGCGACACGAGTCGACCTATTCGCcat                                                                                                                                    >  1:217216/1‑141 (MQ=255)

               aTTGAACCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGt                                                                                                                               >  2:648437/1‑141 (MQ=255)

               aTTGAACCATGATGTCATCAACAATGGTTAGCCCACCGCGCATAGATCATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAGATCCAGCGACACGAGTCGCCATATTCGCcatcagag                                                                                                                               >  2:451676/1‑138 (MQ=255)

                  gAACCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACCACTCCAGCGACACGAGTCGACATATTCGCCATCATGTCat                                                                                                                            >  2:364926/1‑141 (MQ=255)

                    aCCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCatat                                                                                                                          >  2:474600/1‑141 (MQ=255)

                    aCCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCatat                                                                                                                          >  1:804502/1‑141 (MQ=255)

                    aCCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATAGTCGCCATCATGTCatat                                                                                                                          >  1:892136/1‑141 (MQ=255)

                     ccATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATg                                                                                                                         <  2:338816/141‑1 (MQ=255)

                      cATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATgg                                                                                                                        <  2:688562/141‑1 (MQ=255)

                      cATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATgg                                                                                                                        >  1:688562/1‑141 (MQ=255)

                       atgatgTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGt                                                                                                                       <  1:375666/141‑1 (MQ=255)

                         gatgTCATCAACAATGGTTAGCCCACCGCTGTTAGATAATTCAGTATGGAAGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGc                                                                                                                     <  1:344709/141‑1 (MQ=255)

                          atgTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGca                                                                                                                    <  1:12995/141‑1 (MQ=255)

                            gTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGcaac                                                                                                                  >  2:279595/1‑141 (MQ=255)

                            gTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGcaac                                                                                                                  >  2:752814/1‑141 (MQ=255)

                            gTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTAGACATATTCGCCATCATGTCATATGGTGcaac                                                                                                                  >  1:62279/1‑141 (MQ=255)

                                  aacaaTGGTTAGCCCACCGCGCCTAGATAATTCAGTAGGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGCATCGACAAATCCAGCGAAACGAGTCGAAAAATTCCAGACGATTTCATATGGTGCAACAATTAt                                                                                                            <  1:439928/141‑1 (MQ=255)

                                   acaaTGGTTAGCCCACCGCGCTTAGATAATTCGGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACGTATTCGCCATCATGTCATATGGTGCAACAATTATc                                                                                                           >  1:473270/1‑141 (MQ=255)

                                   acaaTGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACGTATTCGCCATCATGTCATATGGTGCAACAATTATc                                                                                                           >  1:586531/1‑141 (MQ=255)

                                    caaTAGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCa                                                                                                          >  2:631149/1‑141 (MQ=255)

                                     aaTGGTTAGCCCACCGCGCTTCGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGCCACATGGTGCCACACTTACCac                                                                                                         >  1:831319/1‑140 (MQ=255)

                                      aTGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCata                                                                                                        >  1:683407/1‑141 (MQ=255)

                                        ggTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATg                                                                                                      <  1:422258/141‑1 (MQ=255)

                                        ggTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATg                                                                                                      >  2:520363/1‑141 (MQ=255)

                                        ggTGAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCAACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATg                                                                                                      <  1:113460/141‑1 (MQ=255)

                                          ttAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGt                                                                                                    >  2:61894/1‑141 (MQ=255)

                                           tggCCCAAAGCGCTTGGATAATTCAGTCTGGACGATTAGTGGTTCTGGCAGTTGGTTCGCGAGCAAAGTATCGACAAATCCAGAGACACGAGTCGACATATTAGGCATTATGTCATATGGTGCAACAATTTTCATATGGtt                                                                                                   >  1:314573/3‑141 (MQ=255)

                                           tAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGtt                                                                                                   >  1:354628/1‑141 (MQ=255)

                                            aGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTtg                                                                                                  >  2:797185/1‑141 (MQ=255)

                                              cccACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTtgtg                                                                                                <  1:728429/141‑1 (MQ=255)

                                              cccACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTtgtg                                                                                                <  1:346962/141‑1 (MQ=255)

                                              cccACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTtgtg                                                                                                <  1:91230/141‑1 (MQ=255)

                                                cACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGaa                                                                                              <  1:220933/141‑2 (MQ=255)

                                                 aCCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGAcc                                                                                             <  1:628120/141‑1 (MQ=255)

                                                 aCCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGAcc                                                                                             <  2:820769/141‑1 (MQ=255)

                                                 aCCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGAATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGGTGAGAcc                                                                                             >  2:443826/1‑141 (MQ=255)

                                                      gcTTAGAAAATTCAGTATGGACGATTTGTGGCTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAACCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGtt                                                                                        <  1:145607/141‑1 (MQ=255)

                                                         tAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTTTa                                                                                     >  2:603887/1‑141 (MQ=255)

                                                          aGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCCTCATGTCATATGGTGCAACACTTATCACATGGTCGTGACCGAGTTCtct                                                                                    >  2:212676/1‑139 (MQ=255)

                                                            aTAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTTGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTa                                                                                  <  2:707805/141‑1 (MQ=255)

                                                                 tCAGTATGAAAGGTGTGTGGTTCTGGCAACTGCCCCACGCGCAAAGTCTCGGTCCATCCAACCACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAAtgt                                                                             >  2:877838/1‑141 (MQ=255)

                                                                   aGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCTGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGGTGTGACCGCGTTCTATTAAAtgttg                                                                           >  1:37152/1‑141 (MQ=255)

                                                                   aGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCAAGCGACCAGAGTCGAAAAATTAGCCATCATGTCATGTGGTGCACCCATTATCATATGGTTGTGACCGAGTTCAATTAAAtgttt                                                                           >  2:15316/1‑140 (MQ=255)

                                                                    gTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGAAAAATCCAGGGACACGAGTCGGCCTATTGGCCATTATGTCATATGGTGCAAAAATTATCATATGGTTGTGACCGAGCTCTATCAAAggttag                                                                          >  2:89387/1‑139 (MQ=255)

                                                                     tATGGACTGTTTGTGGTTCTGGCAAGTGATTGGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTtgtg                                                                         <  1:464833/141‑1 (MQ=255)

                                                                     tATGGACGATTTGTGGTTCTGGGAATTGATTTCTTGGCAAAGTATAGACAAATCCAGCGACACGAGTGGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTtgtg                                                                         <  1:275700/141‑1 (MQ=255)

                                                                     tATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTtgtg                                                                         <  1:420601/141‑1 (MQ=255)

                                                                        ggACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGtgctg                                                                      <  2:294113/141‑1 (MQ=255)

                                                                           cGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAAGCCAGCGACACGAGTCGACGTATTCGCCATCCTGTCATAGGGTGCCACAATTATGCTATGGTTGTGCCCTGGTTCTAATGAAGGTTGGGCTGCaa                                                                   >  1:48373/1‑141 (MQ=255)

                                                                             aTTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATAGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGt                                                                 >  2:574481/1‑141 (MQ=255)

                                                                              tttGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGATGTGCTGCAAGtt                                                                >  2:655584/1‑141 (MQ=255)

                                                                               ttGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGttt                                                               >  1:871024/1‑140 (MQ=255)

                                                                               ttGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCACATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTTTGCTGCAAGttt                                                               >  1:659714/1‑140 (MQ=255)

                                                                                tgtgGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTCCAACAATTATCATATGGTTGTGACCGAGTTCTGTTAAATGGTGTGCTGCAAGTTGt                                                              >  2:545195/1‑141 (MQ=255)

                                                                                tgtgGTCCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGt                                                              <  2:537413/141‑1 (MQ=255)

                                                                                 gtgGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTc                                                             <  1:749063/141‑1 (MQ=255)

                                                                                  tgGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGGCCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTcc                                                            >  1:461822/1‑141 (MQ=255)

                                                                                      tCTGGCAAGTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCt                                                        <  2:573969/141‑1 (MQ=255)

                                                                                            aaTTGCTTCGCGGGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGGTCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGatat                                                  <  2:259899/141‑1 (MQ=255)

                                                                                             aTTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATAtt                                                 >  2:603886/1‑141 (MQ=255)

                                                                                                    cgcgcgCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGt                                          >  1:401387/1‑141 (MQ=255)

                                                                                                     gcgcgcAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCAGATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCCTTTGTc                                         >  2:230419/1‑141 (MQ=255)

                                                                                                         gcAAAGTATCGACAAATCCAGCGACACGGGTCGACATATTAGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGaa                                     <  2:463675/141‑1 (MQ=255)

                                                                                                         gcAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGaa                                     <  2:526434/141‑1 (MQ=255)

                                                                                                          cAAAGTATCGACAAATCCAGCGACACGAGTCGACGTATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGCCCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAAc                                    <  2:473270/141‑1 (MQ=255)

                                                                                                          cAAAGTATCGACAAATCCAGCGACACGAGTCGACGTATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAAc                                    <  2:473206/141‑1 (MQ=255)

                                                                                                          cAAAGTATCGACAAATCCAGCGACACGAGTCGACGTATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAAc                                    <  2:586531/141‑1 (MQ=255)

                                                                                                          cAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAAc                                    >  1:107166/1‑141 (MQ=255)

                                                                                                          cAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAAc                                    >  2:707322/1‑141 (MQ=255)

                                                                                                            aaGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACaa                                  <  1:212735/141‑1 (MQ=255)

                                                                                                            aaGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTACCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACaa                                  <  2:678892/141‑1 (MQ=255)

                                                                                                                 tCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCt                             <  1:570855/141‑1 (MQ=255)

                                                                                                                   gACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCTGt                           <  1:241873/141‑1 (MQ=255)

                                                                                                                    tcAAATCCCGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGTAACAATTATCATATGGTTGTGACCGAGTTCTAATAGATGTTGGGCTGCAATTTGTCCACCTTGATATTCATTTGTCCGAACAAAATTTGta                          <  2:552826/140‑1 (MQ=255)

                                                                                                                    aCAAATCCAGCGACACGAGTCGACATATTCGGCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCTGta                          <  2:156948/141‑1 (MQ=255)

                                                                                                                       aaTCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGACCAAAATCTGTATAg                       <  1:474600/141‑1 (MQ=255)

                                                                                                                       aaTCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCTGTATAg                       <  1:580775/141‑1 (MQ=255)

                                                                                                                        aTCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCTGTATAGc                      >  2:169911/1‑141 (MQ=255)

                                                                                                                          ccAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCTGTATAGCCt                    <  1:752814/141‑1 (MQ=255)

                                                                                                                          ccAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCTGTATAGCCt                    <  1:279595/141‑1 (MQ=255)

                                                                                                                             gCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCTGTATAGCCTTGa                 <  2:775052/141‑1 (MQ=255)

                                                                                                                             gCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCTGTATAGCCTTGa                 <  1:364926/141‑1 (MQ=255)

                                                                                                                                gcacGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGCGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCTGTATAGCCTTGATgg              <  2:201001/140‑1 (MQ=255)

                                                                                                                                acacGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCTGTATAGCCTTGATgg              <  1:150592/141‑1 (MQ=255)

                                                                                                                                 cacGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCTGTATAGCCTTGATGGt             <  1:520363/141‑1 (MQ=255)

                                                                                                                                      gTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTGTTAACTGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCTGTATGGCCTTGATGTTCAttt        >  2:249514/1‑141 (MQ=255)

                                                                                                                                            atatTCGCCATCATGTCATATGGTGCACCAATTATCATATGCTTGTGCCCGCATTCTATTAAATGTTGTGCTGCAAGTTGTCCACTTTGATATTCATTTGTCCGAACAAAATCTGTATAGCCTTGATGGTCATTTTGATcc  <  2:876834/141‑1 (MQ=255)

                                                                                                                                            atatTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTGGATATTCATTTGTCCGAACAAAATCTGTATAGCCTTGATGGTCATTTTGATcc  <  2:782136/141‑1 (MQ=255)

AGATTGCAGTGGCAGATTGAACCATGATGTCATCAACAATGGTTAGCCCACCGCGCTTAGATAATTCAGTATGGACGATTTGTGGTTCTGGCAATTGATTCGCGCGCAAAGTATCGACAAATCCAGCGACACGAGTCGACATATTCGCCATCATGTCATATGGTGCAACAATTATCATATGGTTGTGACCGAGTTCTATTAAATGTTGTGCTGCAAGTTGTCCACCTTGATATTCATTTGTCCGAACAAAATCTGTATAGCCTTGATGGTCATTTTGATCC  >  CP000730/317151‑317431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`