Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,814 Δ1 bp 39.7% intergenic (+45/+63) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8140A.39.7% 113.6 / 106.4 68intergenic (+45/+63)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (23/17);  new base . (15/12);  total (39/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.41e-01

TATGCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTAT  >  CP000730/35674‑35953
                                                                                                                                            |                                                                                                                                            
tatGCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATa                                                                                                                                              <  2:334660/141‑1 (MQ=255)
             cTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAatgttatgttt                                                                                                                                 >  1:78814/1‑140 (MQ=255)
              tGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGc                                                                                                                                <  2:226445/141‑1 (MQ=255)
                aaTTTAAATATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCaa                                                                                                                              <  2:85169/141‑1 (MQ=255)
                   ttAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAACATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGACGATTAAGTTGCaaata                                                                                                                           >  2:296910/1‑139 (MQ=255)
                   ttAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTGACGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGCAGGTCCTGTTGCAAAGTa                                                                                                                          >  2:132492/1‑141 (MQ=255)
                     aaaGAACTAGAACATCTCATTGAAAAAGTATTTAATGTAGAAGCTATTCAGTAAAAAGTTTTTGGCGAAAAATGATTAATTGAACAAGAGCTAAAATAAAATTGTGATCTAATAACAATAGAAGGTTCTGTTGCAAAGTaa                                                                                                                         <  2:117386/141‑1 (MQ=255)
                       aaaTGTAGATGATCTCAATTACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaa                                                                                                                       <  2:298009/139‑1 (MQ=255)
                            tAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGCATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGGAGGTTCTGTTGCAAAGTaaaaaatat                                                                                                                  >  1:217198/1‑138 (MQ=255)
                             aGATGATCTCATTGACAAAGTATTTATTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGATAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCAGTTGCAAAGTAAAAAAatat                                                                                                                 <  1:216659/141‑1 (MQ=255)
                             aGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAatat                                                                                                                 <  1:84340/141‑1 (MQ=255)
                              gatgatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATCACGTTTTTGGCGAAAATTGATTACTTCTACGCGCGGTGCAACACACTGGTGGGCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAAtata                                                                                                                >  2:343907/1‑141 (MQ=255)
                              gatgatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAGAAAAAtata                                                                                                                >  2:44555/1‑141 (MQ=255)
                                     tcATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAAcc                                                                                                         <  1:104874/141‑1 (MQ=255)
                                       aTTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAc                                                                                                       <  1:241880/141‑1 (MQ=255)
                                       aTTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAc                                                                                                       >  2:133359/1‑141 (MQ=255)
                                               aGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTAt                                                                                               >  2:309625/1‑141 (MQ=255)
                                                      aaTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACAACTAATTTATCATGTCa                                                                                        >  1:123909/1‑141 (MQ=255)
                                                             aaGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCg                                                                                 >  2:29741/1‑141 (MQ=255)
                                                             aaGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCg                                                                                 <  1:142358/141‑1 (MQ=255)
                                                              aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                               >  2:175547/1‑141 (MQ=255)
                                                               gCTATTCAGTATAAAGTTTTTGGCGAGAAATGATTACTTGAACAAGAGCTAAAATAAATATGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTTGCt                                                                              >  2:177437/1‑141 (MQ=255)
                                                               gCTATTCAGTATAAAGTTTTTGGAGAAAAATGATTACTTGAACTAGAGCTAAAATTAAATTGTGATTTAATAAAACTAGAAGGTTATGTTGCATAGTAATAGAATATAGCTAAGCACTAATTTATCATGTCGGTGTTCgat                                                                               >  2:268054/1‑139 (MQ=255)
                                                                cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtt                                                                             <  1:289245/141‑1 (MQ=255)
                                                                cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCtt                                                                             >  2:275276/1‑141 (MQ=255)
                                                                  atgcAGTAGAAAGTTTTTGGCGACAAATGATTACTTGAACAAGAGCTAAAATAAAATCGGGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATCTAGCTACCCAATAATTGAACAGGGCAGCATACGCTTaa                                                                           >  2:193245/4‑141 (MQ=25)
                                                                           aaaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGc                                                                  >  1:121688/1‑141 (MQ=255)
                                                                                    ggCGAAAAATGATTACTTGAACAAGAGCTACAATAAAATTGTGATATCATCAAAATCGACGATACTGTCGCAAAGTAAAAAAATATAGTTAACCACTAATTAATCAGGTCACGGTTCGCTTAACTGGCTAGCATGATGATa                                                         >  2:262252/1‑141 (MQ=18)
                                                                                          aaaTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCg                                                   >  2:118006/1‑141 (MQ=255)
                                                                                               cttACTTGAACACGAGCTAAAATAAAATTGTGATCTACTAAAAATAGAAGGCTGTGTGGCGAGGTCAACACATATACATAGCCAATAATTTATAAGGTTAGTGTTCGCTTAACTTCGTTGCATGACGCTAATTTctgggcc                                              >  2:154662/2‑135 (MQ=255)
                                                                                                    ttGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCg                                         <  2:276320/141‑1 (MQ=255)
                                                                                                            gagCTAAAATAAACATGTGATCTAATAAAAAAAGAAGGTTCTGTTGCAAAGTAAAAAAAGATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAAATTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                 >  2:12374/1‑141 (MQ=17)
                                                                                                               cTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAg                              >  2:356805/1‑141 (MQ=255)
                                                                                                                taaaataaaaTTGTGATCCAATAAAAATAGAAGGTTCTGTTGCAAAATAAAAAAATATAGCTAGCCACTAATTTATCATGTCAGTGTTAGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGa                             >  1:158792/1‑141 (MQ=255)
                                                                                                                 aaaataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGTGCTACCATGTACCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGAt                            >  1:116326/1‑141 (MQ=17)
                                                                                                                 aaaataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGAt                            <  2:121688/141‑1 (MQ=255)
                                                                                                                      aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGTCGAAAATCCACACATCTGAaga                     >  2:142730/13‑141 (MQ=11)
                                                                                                                        aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                   >  1:43498/11‑141 (MQ=11)
                                                                                                                        aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                   <  1:289486/131‑1 (MQ=11)
                                                                                                                         agattattttaataaAAATGATGGTTCTGTTGCAAAGTAAAATCTATCGATACTATTTTATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                  <  2:343690/132‑1 (MQ=2)
                                                                                                                         agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAagaggc                  >  1:97609/10‑139 (MQ=11)
                                                                                                                         agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                  <  2:156940/132‑1 (MQ=11)
                                                                                                                         agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                  <  2:191624/132‑1 (MQ=11)
                                                                                                                         agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                  >  1:68271/10‑141 (MQ=11)
                                                                                                                         agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTACTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                  >  2:195761/10‑141 (MQ=11)
                                                                                                                          gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                 <  2:346702/133‑1 (MQ=11)
                                                                                                                          gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                 <  2:336269/133‑1 (MQ=11)
                                                                                                                           tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                  <  1:246851/141‑1 (MQ=255)
                                                                                                                           tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                  <  1:29741/141‑1 (MQ=255)
                                                                                                                           tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCAAAATTGTTTCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                  <  1:352139/141‑1 (MQ=14)
                                                                                                                           agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                <  2:42724/134‑1 (MQ=11)
                                                                                                                           agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGCCGAAAAGCCGTAGATCTGAAGAGACCt                <  2:164678/134‑1 (MQ=11)
                                                                                                                            gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg               >  1:166320/7‑141 (MQ=11)
                                                                                                                            gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg               <  1:83195/135‑1 (MQ=11)
                                                                                                                             tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATGACTAATATATAATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCCTGGCGAAGAGCCGTAGATCTGAAGTGACATgg              >  2:271487/6‑140 (MQ=11)
                                                                                                                             tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc              <  1:295848/136‑1 (MQ=11)
                                                                                                                              attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg             >  1:254896/5‑141 (MQ=11)
                                                                                                                               ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg            <  1:140873/138‑1 (MQ=11)
                                                                                                                               ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg            >  2:184893/4‑141 (MQ=11)
                                                                                                                               ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGACCTGAAGAGACCTGCgg            >  1:86939/4‑141 (MQ=11)
                                                                                                                                tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt           >  1:229285/3‑141 (MQ=11)
                                                                                                                                tCTAATAAAAATAGAAGGTTTTGTTGAAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg             <  2:1698/141‑1 (MQ=255)
                                                                                                                                tCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg             <  1:106863/141‑1 (MQ=255)
                                                                                                                                 ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCGCTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt          >  2:269101/2‑141 (MQ=11)
                                                                                                                                 ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCTTGTTAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGTAAATTCGTAGGTTTGATGAGACCTGCgggt          >  2:296495/2‑139 (MQ=11)
                                                                                                                                 ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTGGATCTGAAGAGACCTGCGGtt          >  2:144807/2‑141 (MQ=11)
                                                                                                                                 ttaataaAAATGATGGTTCTGTTACAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt          >  2:319691/2‑141 (MQ=11)
                                                                                                                                  taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc         <  2:295532/141‑1 (MQ=11)
                                                                                                                                           tAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat  >  2:152680/1‑141 (MQ=32)
                                                                                                                                            |                                                                                                                                            
TATGCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTAT  >  CP000730/35674‑35953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: