BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,269	C→T	56.1%	A95T (GCA→ACA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,269	0	C	T	56.1%	43.9 / inf	384	A95T (GCA→ACA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (95/71); new base T (102/111); total (201/183)
Fisher's exact test for biased strand distribution p-value = 7.85e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 386 total aligned reads displayed.

GCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36130‑36409

tcTACCTTCGTTTAAGGTGCCTGATCTGTAATTACCTTTTTAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCa                                                                                                                                             <  2:6207/140‑1 (MQ=17)

 cTACCTTCTTTGACGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAACGATTATCTCGTTGCTTACGCAACCAAATGCTTAATTTATGTCCCTCTGTAt                                                                                                                                            <  2:254589/141‑1 (MQ=11)

  tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc                                                                                                                                           >  1:136836/1‑141 (MQ=32)

   gccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCa                                                                                                                                          >  2:8790/2‑141 (MQ=12)

   aCCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCa                                                                                                                                          <  2:274646/141‑1 (MQ=32)

      ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAATATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCCATg                                                                                                                                       >  2:197048/1‑141 (MQ=17)

          ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCAc                                                                                                                                   >  1:63515/1‑141 (MQ=32)

          ttGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTTTCAATTGCAc                                                                                                                                   >  1:127261/1‑141 (MQ=11)

            gAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGa                                                                                                                                 >  1:192826/1‑141 (MQ=255)

              aGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGata                                                                                                                               >  2:120760/1‑141 (MQ=17)

              aGGTGCCTGACTTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGACAAACGCATATGCTGAATGATTATCGCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGata                                                                                                                               <  2:333852/141‑1 (MQ=255)

                   ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTTCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTCTCAATGGCACGATATAAAt                                                                                                                          >  1:284481/1‑141 (MQ=12)

                     tGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACgtcttaaatcg                                                                                                                        >  1:23328/1‑131 (MQ=12)

                       aTCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGcc                                                                                                                      >  1:223042/1‑140 (MQ=17)

                       aTCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGcc                                                                                                                      >  1:274698/1‑140 (MQ=255)

                         cTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTACTATATGTCCCTCAGCATCAATGACACGATATAAAtcaacca                                                                                                                    >  2:131926/1‑135 (MQ=11)

                         cTGTAATTACCTTTTGAGGTTTACCAAATTGTTGAATGCGAAGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTcc                                                                                                                    <  1:242824/141‑1 (MQ=255)

                             aactatcTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAttt                                                                                                                <  1:39348/135‑1 (MQ=255)

                              aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtttt                                                                                                               >  2:56093/1‑141 (MQ=17)

                               ttACCTTTTGCGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTc                                                                                                              <  2:59690/141‑1 (MQ=17)

                                 acgttttGTGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCt                                                                                                            <  1:209873/138‑1 (MQ=17)

                                  ccTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCCACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAACTAGCTCCATTTTCCtt                                                                                                           >  2:87519/1‑141 (MQ=255)

                                   ctattGACGCTTACCAAATTGATTAATGAGACGTTTCATAAACGCATATGCCGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                          <  2:214119/138‑1 (MQ=11)

                                   cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGAAATAAATAGCTCCATTTTCCttt                                                                                                          >  1:276819/1‑141 (MQ=17)

                                     tttGAGGTTTACCAAATCGTTTAATGAGACGTTTGATAAACGCTTATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTa                                                                                                        <  1:130618/141‑1 (MQ=12)

                                      ttGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAt                                                                                                       >  2:74414/1‑141 (MQ=12)

                                        gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCCCGATATAAATAGCTCCTTTTTCCtttttttt                                                                                                     >  1:121147/1‑141 (MQ=255)

                                         aGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTACTTTTAtttt                                                                                                    >  1:102867/1‑141 (MQ=17)

                                          ggTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTg                                                                                                   >  1:335892/1‑141 (MQ=17)

                                           gcttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTgt                                                                                                  >  1:297895/3‑140 (MQ=17)

                                             ttACCAAATTGTTTAATGGGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAta                                                                                                <  1:114245/141‑2 (MQ=17)

                                             ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTgcta                                                                                                >  1:161364/1‑138 (MQ=17)

                                             ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCACTGGCACGATATAAATAGCTCCATTTTCCTTTTCTTTTGATg                                                                                                >  2:204933/1‑141 (MQ=17)

                                               aCCAAATTGATTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCGTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTa                                                                                              <  2:231222/141‑1 (MQ=17)

                                                 cAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAAGGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACg                                                                                            >  1:47113/1‑141 (MQ=17)

                                                   aaTTGTTTCATCACACGTTTGATTTACTTATAGGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtc                                                                                          <  2:267593/141‑1 (MQ=11)

                                                     ttGTTTAATGAGACTTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtctc                                                                                        <  1:256606/141‑1 (MQ=12)

                                                     ttGTTTAATCAGACGTTTGATAAACTCATATGCTTAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtctc                                                                                        <  1:83154/141‑1 (MQ=11)

                                                      tGTTTAATGAGACGTTTGATAAACGCATATGATGAATGATTATCTCGTTGCTTACGCAACCTAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTATACGTCtca                                                                                       >  2:48312/1‑141 (MQ=11)

                                                       gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATCTAAATAGCTCCATTATCCTTTTAttttttgttacttctcac                                                                                      >  1:26636/1‑127 (MQ=11)

                                                       gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGAAACCAAATTTCTCTTGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCATTTTTTTTTGTTGTACGtatctt                                                                                      >  1:201333/1‑139 (MQ=11)

                                                           aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACTTCTCCTCaaa                                                                                  >  2:247241/1‑140 (MQ=255)

                                                           aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                  <  1:257114/141‑1 (MQ=17)

                                                           aaTCAGACGGTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                  <  1:97113/141‑1 (MQ=17)

                                                            aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATa                                                                                 >  2:39099/1‑141 (MQ=17)

                                                             tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCCACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAc                                                                                <  2:111032/141‑1 (MQ=17)

                                                               ggaCGTTTGATAAACGCATATGCTGAATGATTATCTCGGTGCTTACGCGACGAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGc                                                                              <  1:169074/140‑1 (MQ=11)

                                                                    tttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAAAGTATGTACCTCTGCATCAATGGCACGATATAAATAGCGCCGGTTTCCTTTGATTTTGATGTACGTCTCATCCACACGCCAttt                                                                         >  2:126873/1‑141 (MQ=11)

                                                                     ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTg                                                                        >  1:325096/1‑141 (MQ=17)

                                                                     ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAAGCCATTTg                                                                        >  1:291938/1‑141 (MQ=12)

                                                                      tGATAAACGGATATGCTGGATGATTATCCCGTTGCTTACGCCATCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTTCATTTTCTTTTTCTTTGGATGTACATCTCATCAAGACGCCAATTGt                                                                       <  2:217718/141‑1 (MQ=11)

                                                                      tGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCCTTTTCCTTTTATTTTGATATACGTCTCATCAATAAGCCCTTTGt                                                                       >  1:247797/1‑141 (MQ=11)

                                                                      tGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCAttttt                                                                       >  1:34408/1‑139 (MQ=12)

                                                                       gATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtat                                                                                               <  1:147886/116‑3 (MQ=12)

                                                                       gATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTGTACGTCTCATCAATAGGCCATTAGta                                                                      >  1:7160/1‑141 (MQ=12)

                                                                            aCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAg                                                                 >  2:176379/1‑141 (MQ=35)

                                                                             cGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTTTATCCGTCTCATCAAGACGcacgttatgatacgc                                                                >  1:59215/1‑127 (MQ=11)

                                                                              gCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCATCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTATCATCAATACGCTCTCTCTAATAAGCt                                                               >  2:350338/1‑141 (MQ=11)

                                                                                atatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCttt                                                             <  2:336810/141‑1 (MQ=35)

                                                                                    gCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTACGTTTCATCAATACGCAGTTTGTAATAAGCttttttt                                                         >  1:315976/1‑140 (MQ=11)

                                                                                    gCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCttttttt                                                         >  1:306752/1‑140 (MQ=25)

                                                                                           gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                  <  2:226735/141‑1 (MQ=39)

                                                                                           gATTATCTCGTTGCCTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                  <  1:91625/141‑1 (MQ=255)

                                                                                             ttATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCACTGGCACGATATAAATAGCTCCATTTTCCTTTTCTTTTGATGTACGTCTCATCAATTCGCCATTTGTAATAAGCTTTTTTATGCTTTttc                                                >  1:156032/1‑141 (MQ=255)

                                                                                              tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTATACGTCTCATCAATACGCCATTTTTAATAAGCTTTTTTATGCTTTttct                                               >  2:106959/1‑141 (MQ=255)

                                                                                              tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                               >  2:148196/1‑141 (MQ=40)

                                                                                               aTCTCGTTGCTTACGCAACCCAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTCCATTGTGGTGATCCTCAACTCAGAATATCATTTGTAATAAGCTTTTTTATGCTTTttctt                                              <  1:353619/141‑1 (MQ=14)

                                                                                                tctcGTTGCTTACGCCACCAAATATATAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATGACTCCGCCATTTGTAATAGGCTTTTTTATGCTTTttcttc                                             >  2:30640/1‑141 (MQ=14)

                                                                                                tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCATTACGCCATTTGTAATAAGCTTTTTTATGATTTtttttc                                             >  1:180444/1‑141 (MQ=14)

                                                                                                tcctgTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                             <  2:301009/137‑1 (MQ=255)

                                                                                                cctcGTTTCGTCCGCAAACAAATATCTAATGTATGTCCCTCTGTATAAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                             <  2:142590/140‑1 (MQ=14)

                                                                                                 ctcctcgCTTAACCAACCAAATATCTAATGTATTTCCCTCTGTACCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                            <  1:122792/135‑1 (MQ=11)

                                                                                                 ctcGTTGCTTACGGTACTAAATAGCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAATCTTTTTTTTGCTTTTTCTTcc                                            >  2:173453/1‑141 (MQ=14)

                                                                                                    gTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCaaa                                         >  2:192789/1‑141 (MQ=40)

                                                                                                      tGCTTACGCAACCAAATATCTAATGTATGTCACTCTGCATCAATGGCACGATATAAATAGCTCCGTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAtt                                       >  2:13687/1‑141 (MQ=255)

                                                                                                       gCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATAAAAACGCCATTTGTAATAAGCTTTTTTCTGCTTTTTCTTCCAAAttt                                      >  2:355795/1‑141 (MQ=255)

                                                                                                        cTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAATTg                                     >  2:240126/1‑141 (MQ=255)

                                                                                                          tACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCGCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTggt                                   >  2:287767/1‑139 (MQ=255)

                                                                                                          tACGCAACCAAATATCTAATGTAGGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATATACGTCTCATCAATTCGCCATTTGTATTAAGCCTTTTTATGCGTTTTCTTCCAAAtttact                                   >  1:312649/1‑138 (MQ=11)

                                                                                                            cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGCCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  2:325096/141‑2 (MQ=255)

                                                                                                            cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                 <  1:253458/141‑2 (MQ=39)

                                                                                                                aCCAAATATCAAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaaa                             <  1:19798/141‑1 (MQ=255)

                                                                                                                      tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                       <  2:284481/141‑1 (MQ=255)

                                                                                                                       atCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGc                      <  1:214587/141‑1 (MQ=255)

                                                                                                                        tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTTTTTTGCTTTACGTCTCATCAATACGCCATTTGTAATAAACTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggtct                     >  1:48702/1‑138 (MQ=11)

                                                                                                                        acTAATGTATGTCCCTCTGAATCAATGGCACGATATAAATAGCTCCATTTTCCTATTATTTTGATGTACGCCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAATTGATACCACATTgtggag                     >  2:161356/2‑139 (MQ=14)

                                                                                                                         ttaagtcatatgccTCTGCATCTATGGCACGATATAATCAGCTCCATTTTCCTTTTATTTTAATGTACGTCTCATTAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTCATATAAAATTGGGGCat                    <  2:186347/129‑1 (MQ=11)

                                                                                                                         ttAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                    <  2:309371/140‑1 (MQ=37)

                                                                                                                         ttAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                    <  2:293358/140‑1 (MQ=37)

                                                                                                                          tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTAGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATCTGATCTAAAATTGGGACaaa                   >  2:157151/1‑139 (MQ=11)

                                                                                                                           aaggtatgcCCCTCGGCATCAATAGCACGATATAAATAGCTCCATCTTCCTTTGTTTTAGATGTACGGCTCATCAATACGGCATTTGTAATATGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGTGGCatat                  <  2:90503/138‑1 (MQ=11)

                                                                                                                             tgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATACGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAAAATTGGGGCATATTc                >  2:41409/1‑141 (MQ=255)

                                                                                                                              ttatgtACCTCTGTACCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCCTCCAAATTTGATATAAAATTGGGGCATATTCt               <  2:317533/140‑1 (MQ=11)

                                                                                                                                atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg             <  1:87605/141‑1 (MQ=255)

                                                                                                                                atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg             <  1:143280/141‑1 (MQ=255)

                                                                                                                                 tgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTTGGGCCTATTCTTGa            >  2:101635/1‑141 (MQ=11)

                                                                                                                                 tgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa            >  2:263017/1‑141 (MQ=255)

                                                                                                                                 tctCCCTCTGTATCAATGGCACGATATAAATACCTCCATTTTTCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa            <  1:30409/139‑1 (MQ=11)

                                                                                                                                  gtCCCTCTGTATCAATGGCACAATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAACTTTTTTATGCTTTTTCTTCCCACTTTGATATAACATTGGGGCATATTCTTCaa           >  1:76881/1‑141 (MQ=11)

                                                                                                                                       tctGCTTTAATGGCACGCTATAAAGAGCTCCATTTTCCATCTACGCGGATATTCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCaa      <  1:154287/141‑1 (MQ=11)

GCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36130‑36409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`