Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,315 G→A 54.1% Y79Y (TAC→TAT USA300HOU_0026 ← IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,3150GA54.1% 72.3 / inf 419Y79Y (TAC→TATUSA300HOU_0026IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base G (95/91);  new base A (98/122);  total (202/217)
Fisher's exact test for biased strand distribution p-value = 1.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 423 total aligned reads displayed.

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTC  >  CP000730/36175‑36455
                                                                                                                                            |                                                                                                                                            
ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCACTGGCACGATATAAATAGCTCCATTTTCCTTTTCTTTTGATg                                                                                                                                              >  2:204933/1‑141 (MQ=17)
 tggcgaaTTGTTTAATGAGACGTTTGATAAACGTATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGt                                                                                                                                             <  2:176081/136‑1 (MQ=12)
   ccAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTAc                                                                                                                                           >  1:341641/1‑141 (MQ=32)
      aaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                                                                        <  1:196466/141‑1 (MQ=12)
      aaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtc                                                                                                                                        <  1:46093/141‑1 (MQ=32)
       aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTTATGTATGTCGCTGATTATGAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtct                                                                                                                                       <  1:155931/141‑1 (MQ=11)
        ttGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtctc                                                                                                                                      >  1:213996/1‑141 (MQ=32)
             tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATTTACGTCTCATCaa                                                                                                                                 >  1:289420/1‑141 (MQ=17)
              aaTGAGACGTTTGATAATCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAAt                                                                                                                                <  1:326502/141‑1 (MQ=17)
               aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTTTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCtcattattt                                                                                                                               >  1:35838/1‑136 (MQ=11)
                 gataCGTTTGATAAACGCATATCCTGAATGATGATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACg                                                                                                                             <  2:246521/138‑1 (MQ=11)
                    aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTATACGTCTCATCAATACGcct                                                                                                                          >  1:271093/1‑140 (MQ=12)
                    aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCGATTTTCCTTTTATTTTGATGTACGTCTCATGAATACGCCa                                                                                                                          >  1:253898/1‑141 (MQ=17)
                    aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCATACGCAACCAAATATATAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATCTACTACTCATCAATACGcaa                                                                                                                          >  2:170188/1‑139 (MQ=11)
                      gTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCGCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCCCATCAATACGCCAtt                                                                                                                        >  2:8753/1‑141 (MQ=17)
                        ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGAAACATTTTCCTTTTATTTTGATGTACGTCTCATAAATACGCCAttaa                                                                                                                      >  1:98122/1‑139 (MQ=255)
                         tGATAAACGGATATGCTGGATGATTATCCCGTTGCTTACGCCATCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTTCATTTTCTTTTTCTTTGGATGTACATCTCATCAAGACGCCAATTGt                                                                                                                     <  2:217718/141‑1 (MQ=11)
                         tGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGt                                                                                                                     >  1:315511/1‑141 (MQ=17)
                          gATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTGTACGTCTCATCAATAGGCCATTAGta                                                                                                                    >  1:7160/1‑141 (MQ=12)
                           aTAATCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaa                                                                                                                   <  1:246634/141‑1 (MQ=12)
                             aaaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaata                                                                                                                 <  2:315511/141‑1 (MQ=16)
                              aaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaataa                                                                                                                <  2:48581/141‑1 (MQ=21)
                              aaCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaataa                                                                                                                <  2:136836/141‑1 (MQ=34)
                              aaCGCATATGCTGAATGATTACCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaataa                                                                                                                <  1:351165/141‑1 (MQ=255)
                                cGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGc                                                                                                              <  1:142153/141‑1 (MQ=21)
                                 gCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCt                                                                                                             <  1:292693/141‑1 (MQ=255)
                                       gcggAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTa                                                                                                       <  1:220679/138‑1 (MQ=255)
                                            aTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTCATAAGCTTTTTTATTCtt                                                                                                  >  2:83516/1‑141 (MQ=255)
                                              gATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                                                                <  2:226735/141‑1 (MQ=39)
                                               aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttttt                                                                                               >  2:110039/1‑141 (MQ=40)
                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                             >  2:148196/1‑141 (MQ=40)
                                                  aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                            <  2:297895/141‑1 (MQ=255)
                                                  aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                            >  2:190624/1‑141 (MQ=255)
                                                   tctcGTTGCTTACGCCACCAAATATATAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATGACTCCGCCATTTGTAATAGGCTTTTTTATGCTTTttcttc                                                                                           >  2:30640/1‑141 (MQ=14)
                                                   tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                           >  2:8899/1‑141 (MQ=40)
                                                   cctcGTTTCGTCCGCAAACAAATATCTAATGTATGTCCCTCTGTATAAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                                                                           <  2:142590/140‑1 (MQ=14)
                                                    ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                                                                          >  2:301811/1‑141 (MQ=255)
                                                    ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATAGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                                                                          >  1:180654/1‑141 (MQ=255)
                                                         tGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATCAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCGTCCAAAtt                                                                                     <  2:264663/141‑1 (MQ=255)
                                                         tGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAtt                                                                                     >  1:127748/1‑141 (MQ=39)
                                                          gCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTACGTCTCATAAAAACGCCATTTGTAATAAGCTTTTTTCTGCTTTTTCTTCCAAAttt                                                                                    >  2:355795/1‑141 (MQ=255)
                                                             tACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCGCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTggt                                                                                 >  2:287767/1‑139 (MQ=255)
                                                             tACGCAACCAAATAACTAATGTAGGTCCCTCTGCATCAATGGCACGATATAAATAGCGCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAGCCTTTTTTAGGCTTGTTCTTGCATATTTCAt                                                                                 >  2:57677/1‑141 (MQ=14)
                                                               cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                                                               <  2:330123/141‑2 (MQ=255)
                                                               cGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATat                                                                               <  2:102580/141‑2 (MQ=255)
                                                                    ccAAATCTCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACTTCTCATCAATACGCCATTTGTATTTTGCTTTTTTAGGGTTTTTCTTTCAATTTTGAttttaaat                                                                          >  1:109875/1‑134 (MQ=11)
                                                                    ccAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                          <  1:90013/141‑1 (MQ=255)
                                                                       aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCTTTTGTAATAAGCTTTTTTATGATTTTTCTTTCAAATTTGATATAAAATTgg                                                                       >  1:93655/1‑141 (MQ=11)
                                                                       aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                       <  2:335892/141‑1 (MQ=255)
                                                                         tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                     <  1:12128/141‑1 (MQ=255)
                                                                         tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTGCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTgggg                                                                     <  2:196068/141‑1 (MQ=255)
                                                                           acTAATGTATGTCCCTCTGAATCAATGGCACGATATAAATAGCTCCATTTTCCTATTATTTTGATGTACGCCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAATTGATACCACATTgtggag                                                                   >  2:161356/2‑139 (MQ=14)
                                                                            cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTTTGGCat                                                                  >  1:256962/1‑141 (MQ=11)
                                                                             tAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                                 >  2:174460/1‑141 (MQ=255)
                                                                             tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTAGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATCTGATCTAAAATTGGGACaaa                                                                 >  2:157151/1‑139 (MQ=11)
                                                                               atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTGTACGTCTCTTCAATACGCCATTTGTAATAAGCTTTTTTTTGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCagttt                                                               >  1:302329/1‑137 (MQ=255)
                                                                                    tgtCCCTCTGTATCAATGGCACGGTATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCTTTTGTAATAACCTTTTTTATACGTTTTCTCCCAAAATTTATATCAAATTGGGGAATATTCTTGa                                                          >  1:236308/1‑141 (MQ=11)
                                                                                    tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa                                                          >  2:344867/1‑141 (MQ=25)
                                                                                       cccTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAcc                                                       <  2:183155/141‑1 (MQ=18)
                                                                                          tctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCaa                                                    >  2:114609/1‑141 (MQ=255)
                                                                                            tgtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGGACCCaaag                                                  >  2:93429/4‑139 (MQ=11)
                                                                                            tgtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACg                                                  >  2:273873/4‑141 (MQ=255)
                                                                                             gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                 <  1:56534/139‑1 (MQ=16)
                                                                                             gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAGTTTTATATACGTCTCATCAATACGCCATTTGTAATATTCTTTTTTATGCTCTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                 <  2:123613/139‑1 (MQ=11)
                                                                                              taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATATAAAATTGGGGCATATTCTTGAACCCAACggc                                                >  1:123169/2‑140 (MQ=255)
                                                                                              taTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCAAAATTTGATATAAAATTGGGGCATATTCTGGAACCCACCGGt                                                >  1:252380/2‑141 (MQ=11)
                                                                                                tCAATGGCATGAAATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                              <  1:201765/141‑1 (MQ=255)
                                                                                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATACACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                             >  2:40931/1‑141 (MQ=255)
                                                                                                   aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGAcc                                           <  2:127748/141‑1 (MQ=18)
                                                                                                    tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTGATATAAAACTGGGGCATACTCTGGAACACAACggcaagcca                                          >  1:176044/1‑134 (MQ=11)
                                                                                                       cACGATATAAATAGCTACATTCTCATCCTAGGTTGATATACGTCTCATCAATACGCCATTTGTGATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGGTCGTTg                                       >  1:85732/1‑141 (MQ=11)
                                                                                                        ccGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGACCCCAACGGTAGATCGTTgc                                      <  2:60602/140‑2 (MQ=11)
                                                                                                        aCGATTTAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCAAAACTTGTTATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                      <  1:188528/141‑1 (MQ=11)
                                                                                                        aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                      >  1:16003/1‑141 (MQ=255)
                                                                                                          gATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAat                                    <  1:209785/141‑1 (MQ=255)
                                                                                                           atatAAATAGCTCCATTTTTCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatg                                   <  1:305296/141‑1 (MQ=25)
                                                                                                           atatAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatg                                   <  1:278741/141‑1 (MQ=21)
                                                                                                               aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                               >  2:261879/1‑141 (MQ=255)
                                                                                                                 aTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAc                             >  1:47773/1‑141 (MQ=255)
                                                                                                                         cttttTCTTTTGTTTTGATGTACGTCGCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                     <  2:279408/140‑1 (MQ=255)
                                                                                                                          ttttCCTTTTATTTTGTTATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                    <  2:67847/141‑1 (MQ=255)
                                                                                                                          ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                    <  2:186781/141‑1 (MQ=39)
                                                                                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTTGGGCATATTCTTGAACCTAAAGGTAAAATGTTTAACGATGATAGttttaacc                    >  1:63662/1‑136 (MQ=11)
                                                                                                                           tttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTTGACCGTTGAATGATGAACGTTTAcacca                   <  1:148440/141‑1 (MQ=18)
                                                                                                                           tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                   <  1:171016/141‑1 (MQ=255)
                                                                                                                           tttCCTTTTATTTGGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGTTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                   <  1:78599/141‑1 (MQ=11)
                                                                                                                            ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACATTTGATCTGAAATTGGGGGATAGTCTTGAACCCAACGGTAGTTCTTTGAATGCTGAGCGTTTAcaccac                  >  1:134955/1‑141 (MQ=11)
                                                                                                                            ttCCTTTTATTTTCATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                  <  2:257727/141‑1 (MQ=255)
                                                                                                                             tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTCCACCACg                 >  1:309929/1‑141 (MQ=25)
                                                                                                                              ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTGGATACAAAATTGGGGCATATTCTTGAACCCAAGGGTAGACCGTTGAATGATGAACTTTTACTCCCCGt                >  2:208113/1‑141 (MQ=14)
                                                                                                                              ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                <  1:263017/141‑1 (MQ=255)
                                                                                                                              ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                >  1:60986/1‑141 (MQ=255)
                                                                                                                               cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTCTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtt               >  2:318873/1‑141 (MQ=17)
                                                                                                                               cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGtt               >  1:118271/1‑141 (MQ=255)
                                                                                                                               cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt               >  1:100282/1‑141 (MQ=255)
                                                                                                                                 tttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTct             >  1:166178/1‑140 (MQ=255)
                                                                                                                                  gtATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCACAGGCAGACCGGTGAATGATGAACGTTTACACCACGTTccc            <  1:318551/140‑1 (MQ=11)
                                                                                                                                    aTTTTGATGTACGTCTTATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt          <  1:336624/141‑1 (MQ=255)
                                                                                                                                       ttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCTAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAat       <  2:236118/141‑1 (MQ=21)
                                                                                                                                            gTACGTCTCAGTAATACGCCATTTGTAATAAGCTTTTTTATCCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCACCGGCATACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTc  <  1:157192/141‑1 (MQ=11)
                                                                                                                                            |                                                                                                                                            
TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTC  >  CP000730/36175‑36455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: