| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 478,502 | C→A | 100% | D342E (GAC→GAA) | USA300HOU_0457 → | NADH dehydrogenase (ubiquinone) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 478,502 | 0 | C | A | 100.0% | 111.8 / NA | 35 | D342E (GAC→GAA) | USA300HOU_0457 | NADH dehydrogenase (ubiquinone) |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (15/20); total (15/20) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
AGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCA > CP000730/478364‑478639 | aGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCC‑AAACAAGCATCTGCTAAAGAAGCt > 2:283181/1‑141 (MQ=255) gCATTGGGTGTATATTCAGCCGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAAAATTATTTTTACAATCAGGTTCTCTAGTGAAGCGATTCAATATTCCAAAAAAAGAATCTGCTAAAGAAGCTTATGGTTgg > 1:172849/1‑141 (MQ=255) gCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTgg < 1:230354/141‑1 (MQ=255) gggTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAGAGAAGCTTATGGCTGGATTGt > 1:4489/1‑141 (MQ=255) cGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGCCGTGTATTAg > 2:220999/1‑141 (MQ=255) attattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTTAAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCt < 1:314951/141‑1 (MQ=255) attattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCt < 1:137240/141‑1 (MQ=255) attattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCt < 1:137245/141‑1 (MQ=255) tattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtat < 1:234767/141‑1 (MQ=255) attCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTAGCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtatt > 1:219917/1‑141 (MQ=255) cATTTAATATTGCACGGGATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATc < 1:175596/141‑1 (MQ=255) atTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAtt < 2:330864/141‑1 (MQ=255) gCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCt < 2:7346/141‑1 (MQ=255) gCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCt < 1:71173/141‑1 (MQ=255) gTATGTTTAAAGAAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAtttt > 1:252966/1‑141 (MQ=255) aTTTTTAAAGCAACATTATTTTTTTTTTTTGTTTCTATCGTGAAGCGATTCAATATTCCAAAACAGGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTATCTATTATCGTGGCATTTTTGTTTTgg > 2:113864/1‑141 (MQ=255) ttttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGAt > 2:154439/1‑141 (MQ=255) ttttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGGATTAGCTATTATCGTGGCATTTCTATTTTGGAt > 1:115384/1‑141 (MQ=255) tttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATg > 1:135145/1‑141 (MQ=255) aaGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGagta < 1:114776/141‑1 (MQ=255) aaCAGTATGTTTAGAATCAGGTCTTATCGTGAAGCGATTCAATATTCCAAAATAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGa < 2:38361/141‑1 (MQ=255) ttattTTTACAATCAGGTTCTATCGTCAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAATTTGGATGAGTAGTGACAGa < 1:217516/141‑1 (MQ=255) tttttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGGGTGTTAGCTATTATCGTGGCGTTTCTATTTTGGATGAGTAGTGACAGAAgg > 1:315329/1‑140 (MQ=255) tttttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATAGGACGTGTATTAGCTATTATCGTGGCATGTCTATTTTGGAGGAGTAGTGACAGAAgg > 1:286242/1‑140 (MQ=255) ttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCa < 1:205502/141‑1 (MQ=255) aaTCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATg < 2:135145/141‑1 (MQ=255) aTCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGt < 2:150234/141‑1 (MQ=255) tGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCAc < 1:220999/141‑1 (MQ=255) gAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACt < 1:91980/141‑1 (MQ=255) ttCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCAGAATTGTCAAGGGACGTGAATACGCAATTAACGGGGCATTTATCTTTTGGATGAGCAGTGACAAAAGTGCGTATGGAATGTGAAGTGCatttgttggg > 2:69203/1‑132 (MQ=255) atatTCCAAAAAAAGCATCCGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGCGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGAt < 1:195396/141‑1 (MQ=255) aGCATCTGCTAAAGAAGCTTATGGCTGGATTGTTATGGGACGTGTATTAGCTATTATCGTGGGATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGCAGTGTTAAGTGCAGTCATTCTAGGATGGTGATTATTTGt > 2:129147/1‑141 (MQ=255) cTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATTCAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCtt < 2:252966/141‑1 (MQ=255) cTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTCTTAACGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTCCTTGTATCTTGGa > 1:68795/1‑141 (MQ=255) aGAAGCTTATGGCTGTATTGTCATGGTACGTGTAGTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTGAGTGCACTCATTCTAGGATGGTCTTTACTTGTATCTTGGAATCa < 1:217096/141‑1 (MQ=255) | AGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCA > CP000730/478364‑478639 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |