Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 478,502 C→A 100% D342E (GAC→GAA USA300HOU_0457 → NADH dehydrogenase (ubiquinone)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730478,5020CA100.0% 111.8 / NA 35D342E (GAC→GAAUSA300HOU_0457NADH dehydrogenase (ubiquinone)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/20);  total (15/20)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCA  >  CP000730/478364‑478639
                                                                                                                                          |                                                                                                                                         
aGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAACAAGCATCTGCTAAAGAAGCt                                                                                                                                        >  2:283181/1‑141 (MQ=255)
          gCATTGGGTGTATATTCAGCCGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAAAATTATTTTTACAATCAGGTTCTCTAGTGAAGCGATTCAATATTCCAAAAAAAGAATCTGCTAAAGAAGCTTATGGTTgg                                                                                                                               >  1:172849/1‑141 (MQ=255)
          gCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTgg                                                                                                                               <  1:230354/141‑1 (MQ=255)
               gggTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAGAGAAGCTTATGGCTGGATTGt                                                                                                                          >  1:4489/1‑141 (MQ=255)
                                cGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGCCGTGTATTAg                                                                                                         >  2:220999/1‑141 (MQ=255)
                                  attattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTTAAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCt                                                                                                       <  1:314951/141‑1 (MQ=255)
                                  attattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCt                                                                                                       <  1:137240/141‑1 (MQ=255)
                                  attattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCt                                                                                                       <  1:137245/141‑1 (MQ=255)
                                    tattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtat                                                                                                     <  1:234767/141‑1 (MQ=255)
                                     attCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTAGCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtatt                                                                                                    >  1:219917/1‑141 (MQ=255)
                                        cATTTAATATTGCACGGGATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATc                                                                                                 <  1:175596/141‑1 (MQ=255)
                                                atTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAtt                                                                                         <  2:330864/141‑1 (MQ=255)
                                                   gCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCt                                                                                      <  2:7346/141‑1 (MQ=255)
                                                   gCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCt                                                                                      <  1:71173/141‑1 (MQ=255)
                                                        gTATGTTTAAAGAAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAtttt                                                                                 >  1:252966/1‑141 (MQ=255)
                                                          aTTTTTAAAGCAACATTATTTTTTTTTTTTGTTTCTATCGTGAAGCGATTCAATATTCCAAAACAGGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTATCTATTATCGTGGCATTTTTGTTTTgg                                                                               >  2:113864/1‑141 (MQ=255)
                                                            ttttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGAt                                                                             >  2:154439/1‑141 (MQ=255)
                                                            ttttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGGATTAGCTATTATCGTGGCATTTCTATTTTGGAt                                                                             >  1:115384/1‑141 (MQ=255)
                                                             tttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATg                                                                            >  1:135145/1‑141 (MQ=255)
                                                                 aaGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGagta                                                                        <  1:114776/141‑1 (MQ=255)
                                                                     aaCAGTATGTTTAGAATCAGGTCTTATCGTGAAGCGATTCAATATTCCAAAATAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGa                                                                    <  2:38361/141‑1 (MQ=255)
                                                                         ttattTTTACAATCAGGTTCTATCGTCAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAATTTGGATGAGTAGTGACAGa                                                                <  1:217516/141‑1 (MQ=255)
                                                                            tttttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGGGTGTTAGCTATTATCGTGGCGTTTCTATTTTGGATGAGTAGTGACAGAAgg                                                             >  1:315329/1‑140 (MQ=255)
                                                                            tttttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATAGGACGTGTATTAGCTATTATCGTGGCATGTCTATTTTGGAGGAGTAGTGACAGAAgg                                                             >  1:286242/1‑140 (MQ=255)
                                                                               ttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCa                                                          <  1:205502/141‑1 (MQ=255)
                                                                                   aaTCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATg                                                      <  2:135145/141‑1 (MQ=255)
                                                                                              aTCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGt                                           <  2:150234/141‑1 (MQ=255)
                                                                                                  tGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCAc                                       <  1:220999/141‑1 (MQ=255)
                                                                                                   gAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACt                                      <  1:91980/141‑1 (MQ=255)
                                                                                                          ttCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCAGAATTGTCAAGGGACGTGAATACGCAATTAACGGGGCATTTATCTTTTGGATGAGCAGTGACAAAAGTGCGTATGGAATGTGAAGTGCatttgttggg                               >  2:69203/1‑132 (MQ=255)
                                                                                                              atatTCCAAAAAAAGCATCCGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGCGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGAt                           <  1:195396/141‑1 (MQ=255)
                                                                                                                           aGCATCTGCTAAAGAAGCTTATGGCTGGATTGTTATGGGACGTGTATTAGCTATTATCGTGGGATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGCAGTGTTAAGTGCAGTCATTCTAGGATGGTGATTATTTGt              >  2:129147/1‑141 (MQ=255)
                                                                                                                                cTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATTCAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCtt         <  2:252966/141‑1 (MQ=255)
                                                                                                                                   cTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTCTTAACGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTCCTTGTATCTTGGa      >  1:68795/1‑141 (MQ=255)
                                                                                                                                       aGAAGCTTATGGCTGTATTGTCATGGTACGTGTAGTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTGAGTGCACTCATTCTAGGATGGTCTTTACTTGTATCTTGGAATCa  <  1:217096/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
AGTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCA  >  CP000730/478364‑478639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: