Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 973,213 C→A 100% E175* (GAG→TAG)  USA300HOU_0930 ← possible HAD superfamily hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730973,2130CA100.0% 83.4 / ‑6.5 29E175* (GAG→TAG) USA300HOU_0930possible HAD superfamily hydrolase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (15/13);  total (16/13)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACC  >  CP000730/973085‑973348
                                                                                                                                |                                                                                                                                       
aCCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATCCCAAGTTTTACAATTTCAATGACAGGGAATGGTGGGCCCCAGCGTCGATGCTAAATATGATCGGc                                                                                                                             >  1:19126/1‑141 (MQ=255)
 ccctaTGCAATAATATTATTTCGGTCAATATTTAAAAATTGACTAACTTGCTCAATGCCACTTGCTCTATTAATCTCAAGCTTTACATTTTCAATGAAAGGGAGTGGTGCGCCGCTGCGTCGATGCTAAATATGATCGGCa                                                                                                                            <  2:116454/137‑1 (MQ=255)
 ccGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCa                                                                                                                            <  2:118679/141‑1 (MQ=255)
 ccGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCa                                                                                                                            <  1:167169/141‑1 (MQ=255)
         aataatATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATg                                                                                                                    >  1:230212/1‑141 (MQ=255)
          ataataTTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGa                                                                                                                   >  1:56392/1‑141 (MQ=255)
                  attTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCACCAGCGTCGATGCTAAATATGTTCGGCATAAAAATTAGTagacat                                                                                                           >  2:337338/1‑136 (MQ=255)
                   tttCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATAGGCATAAAAATGCGTAAGCata                                                                                                          >  2:248868/1‑141 (MQ=255)
                      cGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATAttt                                                                                                       <  2:259069/141‑1 (MQ=255)
                      cGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATAttt                                                                                                       <  2:20011/141‑1 (MQ=255)
                         tCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTCATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATCAAAATGATTAATCataatatgt                                                                                                    >  2:232040/1‑135 (MQ=255)
                                           cTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACtt                                                                                  >  1:210957/1‑141 (MQ=255)
                                                       aTGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCa                                                                      >  2:17048/1‑141 (MQ=255)
                                                         gCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAAt                                                                    >  1:220763/1‑141 (MQ=255)
                                                                   ttattaATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAg                                                          <  2:292316/141‑1 (MQ=255)
                                                                         aTACCAAGGTTTTCAATTTCAATGACAGGGAATGGTGCGCGCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAggg                                                    <  1:97917/141‑1 (MQ=255)
                                                                           aCCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGa                                                  >  1:348246/1‑141 (MQ=255)
                                                                              aaGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTc                                               <  1:49446/141‑1 (MQ=255)
                                                                              aaGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTc                                               <  1:298962/141‑1 (MQ=255)
                                                                               aGTTTTACAATTTCAATGACAGGGAATGGTGCGCACCAGAGTCGATGAAAAATGGTGTTTGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCt                                              <  1:10420/141‑1 (MQ=255)
                                                                                  tttACAATTTCAATGACCGGGGATGAGGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTc                                           >  2:77127/1‑141 (MQ=255)
                                                                                  tttACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATGTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTc                                           >  1:260081/1‑141 (MQ=255)
                                                                                               tGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTa                              <  2:325543/141‑1 (MQ=255)
                                                                                               tGACAGGGAATGGTGCGCCCCAGCGTCGATGCTAAATACGAACGTCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTa                              <  1:97858/141‑1 (MQ=255)
                                                                                                         tGGTGCGCCCAAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGt                    <  1:313414/141‑1 (MQ=255)
                                                                                                          ggTGCGCCCCAGCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCTGtt                   >  2:67184/1‑141 (MQ=255)
                                                                                                                     gCGTCGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTgg        >  2:196933/1‑141 (MQ=255)
                                                                                                                         cGATGCTAAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTa    >  1:75629/1‑141 (MQ=255)
                                                                                                                           aTGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATCTCAGGAAGTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTATGGGATTCTTTCAAGTGGAAAAGTAAATTACCAGTTTGAATTCTTGGATTccc  >  2:21754/1‑141 (MQ=255)
                                                                                                                                |                                                                                                                                       
ACCGAATGCAATAATATTATTTCGGTCAATATTTAAAAATTGTCTAACTTGCTCAATGCCTCTTGCTTTATTAATACCAAGTTTTACAATTTCAATGACAGGGAATGGTGCGCCCCAGCGTCGATGCTCAATATGATCGGCATAAAAATGAGTAAGCATATTTTTGATTTCAGGTATTTTACTTTCTTCGGCTTCAATTAAAATTGAGGTAGGGGATTCTTTCAAGTGGACAAGTAAATTACCAGTTTGAATTCTTGGATTACC  >  CP000730/973085‑973348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑