Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,194,918 A→G 11.7% intergenic (+973/‑2819) ftsQ → / → USA300HOU_1125 cell division protein FtsQ/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,194,9180AG11.7% 79.5 / 2.5 34intergenic (+973/‑2819)ftsQ/USA300HOU_1125cell division protein FtsQ/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (15/15);  new base G (2/2);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01

ACACTTCTTATGAAACTGCTGAAAAAGTTAAACACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAACTTACTTGGCGTAAAAGAATTATTA  >  CP000730/1194781‑1195058
                                                                                                                                         |                                                                                                                                            
acacTTGTTATGAAACTGCTGAAAAAGTTAAACACCGATATGGTCATGCATTATATGAATCTGCTTCAGATCAAGATATATTCTCTGTTAAACAGGTTGATAGTGATAAAACAGAACAGTATACTCAAAAAAATTTTAGTg                                                                                                                                           >  2:253619/1‑141 (MQ=255)
acacTTCTGATGAAACTGCTGAAAAAGTTAAACACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTCTCGTAGACTCAAAAAGATTTGAGTg                                                                                                                                           <  1:85982/141‑1 (MQ=255)
          tGAAACTGCTGAAAAAGTTAAACACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGa                                                                                                                                 <  2:303370/141‑1 (MQ=255)
             aaCTGCTGAAAAAGTTAAACACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAgc                                                                                                                              <  2:108558/141‑1 (MQ=255)
                   tgAAAAAGTTAAACACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCgtgt                                                                                                                        >  1:110360/1‑141 (MQ=255)
                           ttAACCACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGaaa                                                                                                                <  1:213805/141‑1 (MQ=255)
                             aaaCACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAata                                                                                                              <  1:209414/141‑1 (MQ=255)
                                    aaTATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAGATATTCTTCg                                                                                                       >  1:118892/1‑141 (MQ=255)
                                      tatGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGaa                                                                                                     >  1:93189/1‑141 (MQ=255)
                                               gCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATCCTGACTAAGTTGTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGAt                                                                                            >  1:260410/1‑141 (MQ=255)
                                               gCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGAt                                                                                            <  1:359002/141‑1 (MQ=255)
                                                  ttCTATGATTCTGCTTCAGATCAAGATATCCTCACTGTTAGACAGTTGTATAGGGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGtt                                                                                         <  1:333956/141‑1 (MQ=255)
                                                    cTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGtttt                                                                                       >  1:35913/1‑141 (MQ=255)
                                                         aTTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAg                                                                                  <  1:54051/141‑1 (MQ=255)
                                                              gCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGGGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTa                                                                             >  1:118444/1‑141 (MQ=255)
                                                              gCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGGGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGAGTTa                                                                             >  1:311577/1‑141 (MQ=255)
                                                                     aTCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTaa                                                                      >  2:228774/1‑141 (MQ=255)
                                                                       cAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACa                                                                    >  2:243319/1‑141 (MQ=255)
                                                                        caGATATCTTCACTGTTGAACAGGTTCATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACaa                                                                   <  1:216757/140‑1 (MQ=255)
                                                                            tatCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATGTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGt                                                               >  2:277394/1‑141 (MQ=255)
                                                                                 tCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAAtg                                                          <  2:142245/141‑1 (MQ=255)
                                                                                    cTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTCACAAAAGTAAAtggtg                                                       <  1:212541/141‑1 (MQ=255)
                                                                                       ttGAACAGGTTGAGAGTGATGAACCAGTACAGTATAATCAAAGGGAATTGAGTGGTTTAAGAGAAGCGCGTGTAGAAGAAATATGCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGt                                                    <  2:74858/141‑1 (MQ=255)
                                                                                               gTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTa                                            <  2:311058/141‑1 (MQ=255)
                                                                                                ttGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTaa                                           >  1:113641/1‑141 (MQ=255)
                                                                                                ttGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTaa                                           >  1:198458/1‑141 (MQ=255)
                                                                                                ttGATAGTGATGAAACAGTACAGTACACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTaa                                           >  1:179598/1‑141 (MQ=255)
                                                                                                             aaCAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGc                              <  2:296638/141‑1 (MQ=255)
                                                                                                               caggacaggaTACTCAAAAAGATTTGAGGGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCaa                            >  2:263619/1‑141 (MQ=255)
                                                                                                                  tacagtaTACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAact                         >  2:160143/1‑141 (MQ=255)
                                                                                                                           tgcAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAACTTACTTGGCg                <  2:118892/139‑1 (MQ=255)
                                                                                                                              aaaaaGATTTGGGTGTCTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAACTTACTTGGCGGaa             <  2:311577/141‑1 (MQ=255)
                                                                                                                              aaaaaGATTTGGGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAACTTACTTGGCGTaa             <  2:118444/141‑1 (MQ=255)
                                                                                                                                    aTTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAACTTACTTGGCGTAAAAGAat       >  1:295041/1‑141 (MQ=255)
                                                                                                                                         aGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAACTTACTTGGCGTAAAAGAAttatta  >  1:135048/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                            
ACACTTCTTATGAAACTGCTGAAAAAGTTAAACACCAATATGGTCATGCATTCTATGATTCTGCTTCAGATCAAGATATCTTCACTGTTGAACAGGTTGATAGTGATGAAACAGTACAGTATACTCAAAAAGATTTGAGTGACTTTATTGAAGCGCGTGTAGAAGAAATATTCTTCGAAGTATTTGATGTTTTACAAGATTTAGGATTAACAAAAGTAAATGGTGGGTTTATTGTAACTGGTGGATCTGCAAACTTACTTGGCGTAAAAGAATTATTA  >  CP000730/1194781‑1195058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: