Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,196,508 C→A 100% intergenic (+2563/‑1229) ftsQ → / → USA300HOU_1125 cell division protein FtsQ/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,196,5080CA100.0% 64.0 / NA 23intergenic (+2563/‑1229)ftsQ/USA300HOU_1125cell division protein FtsQ/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/13);  total (10/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGAC  >  CP000730/1196374‑1196645
                                                                                                                                      |                                                                                                                                         
tttCGGTACAGTTATTAGTCCTTAATCACAAGAGTAGAGTGTTGTTAGTGTTATTTCCACTGGTTTTGTTGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCaa                                                                                                                                     <  1:224331/141‑1 (MQ=255)
   cGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAGATACTTATAGCAATGc                                                                                                                                  >  1:359098/1‑141 (MQ=255)
           ttattaATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTa                                                                                                                          <  1:133803/141‑1 (MQ=255)
                        atgaCAAGAATAGCTTGTAGTAACAGTGATTGTAACTGGTTTTTATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGACTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCAtt                                                                                                             <  2:230887/138‑1 (MQ=255)
                        aTTACAATATGAGATTGTCGTAACAGTTATTGCAACAGGTTTTGATCACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCAtt                                                                                                             <  2:144905/141‑1 (MQ=255)
                                                    aTTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATACAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCa                                                                                 <  1:25291/141‑1 (MQ=255)
                                                       gCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAACCAACt                                                                              >  2:281807/1‑141 (MQ=255)
                                                         aaCTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGa                                                                            >  1:307184/1‑141 (MQ=255)
                                                          aCTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGAt                                                                           >  1:201593/1‑141 (MQ=255)
                                                              gTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGTTTTCTAGCAACCGTATATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAgtg                                                                       <  1:244304/141‑1 (MQ=255)
                                                                  tgatgaCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAg                                                                   <  1:35313/141‑1 (MQ=255)
                                                                    atgaCAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTg                                                                 <  1:325670/141‑1 (MQ=255)
                                                                                  tCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACa                                                   <  2:177639/141‑1 (MQ=255)
                                                                                  tCACAAGGTCGTAAAACTGGTAGCACTGGATTCGGAACAAACGTAAATAGTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTAATCAAATGCCCAAGCAACCGATAGTGAAAGTAAAAGAAAACATACa                                                   >  2:17336/1‑141 (MQ=255)
                                                                                     caTGGTCGTAAATCTGGTAGGAGAAGGTCCTTAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACt                                                >  1:85437/1‑141 (MQ=255)
                                                                                                           cTGGATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCt                          <  1:141812/141‑1 (MQ=255)
                                                                                                             ggATTCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTc                        >  2:316670/1‑141 (MQ=255)
                                                                                                                 tCGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTa                    >  2:263132/1‑141 (MQ=255)
                                                                                                                  cGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAGGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAg                   >  1:182173/1‑141 (MQ=255)
                                                                                                                  cGGAACAAGCGTAAATACTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCCAGCTTCATTAg                   >  1:59900/1‑141 (MQ=255)
                                                                                                                                   cTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAACTTCATCAAATGCACAACCAACTGATAGTGTAAGTGAAAGAACACAGACAACTAAATCAGATGATATTCCTAGCTTAATTAGAAATAGAGAAGAAAGAc  <  1:174866/141‑1 (MQ=255)
                                                                                                                                   cTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGCTAAACCAATCGTCATTAGAAATAGAGAAGAAAGAc  <  1:85224/141‑1 (MQ=255)
                                                                                                                                   cTTATAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGAc  <  1:306714/141‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                         
TTTCGGTACAGTTATTAATCCTGAATTACAAGATGAGATTGTTGTAACAGTTATTGCAACTGGTTTTGATGACAAACCAACATCACATGGTCGTAAATCTGGTAGCACTGGATTCGGAACAAGCGTAAATACTTCTAGCAATGCAACTTCTAAAGATGAATCATTCACTTCAAATTCATCAAATGCACAAGCAACTGATAGTGTAAGTGAAAGAACACATACAACTAAAGAAGATGATATTCCTAGCTTCATTAGAAATAGAGAAGAAAGAC  >  CP000730/1196374‑1196645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: