BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,940	G→A	27.3%	R204R (CGC→CGT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,940	0	G	A	27.3%	295.9 / 137.5	233	R204R (CGC→CGT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (82/87); new base A (21/43); total (103/130)
Fisher's exact test for biased strand distribution p-value = 3.84e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 236 total aligned reads displayed.

TGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG  >  CP000730/35799‑36079

tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATGACTAATATATAATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCCTGGCGAAGAGCCGTAGATCTGAAGTGACATgg                                                                                                                                            >  2:271487/6‑140 (MQ=11)

  ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                          <  1:140873/138‑1 (MQ=11)

    ttaataaAAAT‑GATGGTTCTGTTACAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                                                                                        >  2:319691/2‑141 (MQ=11)

     taataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                                                                                                       <  2:295532/141‑1 (MQ=11)

              ttaaaGTTTCTGATGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat                                                                                                                                <  2:134037/138‑1 (MQ=0)

                aaaGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtttata                                                                                                                             <  2:217198/140‑1 (MQ=2)

                                                              tcctGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTAt                                                                                <  1:334428/138‑1 (MQ=1)

                                                                    cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCATTGCCGTATTGATAc                                                                          <  1:266696/141‑1 (MQ=11)

                                                                    cAGTGTTCGTTTAACCTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                          <  1:142114/141‑1 (MQ=2)

                                                                      gttgtCGTTTAACTTGCTAGCGTGATGCTAATTTCGGGGCATGGCGAAAACCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                        <  1:79420/137‑1 (MQ=11)

                                                                       tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                       >  1:319694/1‑141 (MQ=17)

                                                                       tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                       >  2:71151/1‑141 (MQ=17)

                                                                       tgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCTTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                       <  1:207838/141‑1 (MQ=17)

                                                                         ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa                                                                     <  1:312404/141‑1 (MQ=255)

                                                                         ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                     >  1:166023/1‑141 (MQ=17)

                                                                          tcgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGAt                                                                    >  1:147007/5‑141 (MQ=11)

                                                                          tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGAt                                                                    >  1:122447/1‑141 (MQ=255)

                                                                          tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                    >  1:21154/1‑141 (MQ=17)

                                                                           cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                                                   >  2:237111/1‑141 (MQ=17)

                                                                            gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCCTTTAAAGTATTCTTTGCCGTATTGATACTTTGATat                                                                  >  1:139078/3‑140 (MQ=11)

                                                                             tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                 <  1:343440/140‑1 (MQ=17)

                                                                              ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                <  2:142804/141‑1 (MQ=17)

                                                                               tAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCtt                                                               <  1:181970/141‑1 (MQ=11)

                                                                                aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGCGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCttt                                                              >  1:317183/1‑140 (MQ=12)

                                                                                 aCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGt                                                             >  1:303791/1‑141 (MQ=11)

                                                                                 aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTTTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                             <  2:291571/141‑1 (MQ=17)

                                                                                 aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                             <  1:270219/141‑1 (MQ=17)

                                                                                  cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                            <  1:292426/141‑1 (MQ=17)

                                                                                  cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATACAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                                            >  2:259482/1‑141 (MQ=17)

                                                                                   ttGCTAGCATGATGCTAATTTCGTGGCGTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGTGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                           <  2:120120/141‑1 (MQ=12)

                                                                                   ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                           <  1:44555/141‑1 (MQ=32)

                                                                                   ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                           <  1:343907/141‑1 (MQ=32)

                                                                                   ttGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                           <  1:228445/141‑1 (MQ=17)

                                                                                    tgctgtcctgatgTTAATTGCGTGGCATGGGGAAAATCCGTAGATCTTAAGTGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAACTATTCTGTGTCGTATTGATACTTTGATACTTTGtctt                                                          <  2:309804/133‑1 (MQ=11)

                                                                                    tGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACTTACGTTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctt                                                          <  1:92275/141‑1 (MQ=11)

                                                                                    tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAACTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                          <  1:90825/141‑1 (MQ=17)

                                                                                     gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                         <  1:155327/141‑1 (MQ=17)

                                                                                      cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttc                                                        <  1:310238/141‑1 (MQ=17)

                                                                                       tAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                                                       <  2:225577/141‑1 (MQ=255)

                                                                                       tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                       <  1:212475/141‑1 (MQ=17)

                                                                                           atgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTGTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTGTGATACCTTGTCTTTCTTACt                                                   <  1:184893/141‑1 (MQ=16)

                                                                                           atgatgCTAATTTCGCGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTAcc                                                   <  1:329572/141‑2 (MQ=11)

                                                                                            tgatgCTAATTTCGTGGCATGGCGCAAATCCGTAGCTCTGAAGAGACCTGCGGTTCTTTTTGTATAGAGCGTAAATAGCCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGCTACTTTGATACCTTGTCTTTCTTACtt                                                  >  1:21006/1‑141 (MQ=11)

                                                                                             gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                 <  2:21154/141‑1 (MQ=21)

                                                                                             gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                                                 <  2:331288/141‑1 (MQ=25)

                                                                                              tttcTAATTTCGTGGTATGGGGATAATCCGTAAATCTCAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACCTTTAATACCTTTTAAAGTACTCCTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTa                                                <  2:169714/138‑1 (MQ=11)

                                                                                              atgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTa                                                <  1:297830/141‑1 (MQ=255)

                                                                                               tgCCAATTGCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTCCGTTTGATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTaa                                               <  1:10270/141‑1 (MQ=11)

                                                                                                  aaaTTTCGTGGCATGGCGAAAATCCGTAGAGCTGAACATACCTGCGGTTCGTTTTATATAGAGCGTAAATACCCTTAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatat                                            <  2:166890/140‑1 (MQ=11)

                                                                                                   aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATg                                           <  1:37917/141‑1 (MQ=11)

                                                                                                   aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                           >  1:295500/1‑141 (MQ=25)

                                                                                                   aaTTTCGTGGCATGGCGAAAATCCGGAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                           <  1:279712/141‑1 (MQ=25)

                                                                                                      ttCGTGGCGTGGGGAGAATCCGTAGATCTGTAGAGACCTACGGTTCTTTTTATATAGGGCGTAAATACATTCAATACCTTTTAATGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                        <  2:249186/141‑1 (MQ=11)

                                                                                                      ttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                        <  1:190414/141‑1 (MQ=255)

                                                                                                      ttCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATacgacg                                        <  1:243375/141‑1 (MQ=255)

                                                                                                       tCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGTTACTTTGATATTTTGTCTTTCTTACTTTAATATGACgg                                       >  1:59867/1‑141 (MQ=11)

                                                                                                       tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                       <  2:174076/141‑1 (MQ=37)

                                                                                                       tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                       <  1:52068/141‑1 (MQ=38)

                                                                                                        cGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGt                                      <  1:287888/141‑1 (MQ=14)

                                                                                                          tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                    <  1:236366/141‑1 (MQ=255)

                                                                                                           ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                   >  2:353827/1‑141 (MQ=38)

                                                                                                               tGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATCGAGCTTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                               <  2:109257/141‑1 (MQ=14)

                                                                                                               tGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTg                               <  2:256838/141‑1 (MQ=255)

                                                                                                                ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                              >  2:286425/1‑141 (MQ=38)

                                                                                                                 gCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCt                             >  2:205597/1‑141 (MQ=37)

                                                                                                                     aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                         >  2:66520/1‑141 (MQ=38)

                                                                                                                     aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                         >  2:159239/1‑141 (MQ=255)

                                                                                                                     aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                         >  1:290557/1‑141 (MQ=37)

                                                                                                                     aaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTACAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTCCTTTAATATGACGGTGATCTTGCTCAAt                         >  2:39348/1‑141 (MQ=11)

                                                                                                                     aaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTATGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                         <  2:352330/141‑1 (MQ=14)

                                                                                                                      aaTCCGTAGATCTGAAGAGAGCTGCGGTTCTTTTTATATAGAGCATAAATACGCTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTCTCTTCCTTTACTATGACGCTGATCTTGCTCAATg                        >  1:217718/1‑141 (MQ=11)

                                                                                                                       aTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATgg                       >  1:332167/1‑140 (MQ=255)

                                                                                                                          cgcaGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                    <  1:98835/138‑1 (MQ=255)

                                                                                                                          cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATCTAGAGCGTAAATACACTCGATACCTTTTAAAGTATTCTTTGCTGTTTTGATACTTTGATACCTTGTATTTCTTACTTTAATATGACGGTTATCTTGCTCAATGAGGt                    >  2:58371/1‑141 (MQ=11)

                                                                                                                          cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                    <  2:86950/141‑1 (MQ=255)

                                                                                                                           gTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTCCTCAATGAGGtt                   >  2:301601/1‑141 (MQ=37)

                                                                                                                           gTAAATCTGAAGAGCCCTACGGTTCTTTGTATATAGAGCGTATATACATTCAATACCTTCTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATAGGACGGTGATCTTGCTCAATGAGGtt                   <  1:17279/141‑1 (MQ=14)

                                                                                                                            tAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTa                  <  2:229296/141‑1 (MQ=255)

                                                                                                                             aGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTACAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAt                 <  1:206158/141‑1 (MQ=14)

                                                                                                                              gATCTGAAGAGACCTGCGGTTCTTTTTATCTAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                >  1:6207/1‑141 (MQ=255)

                                                                                                                              aaTCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                <  1:73094/140‑1 (MQ=37)

                                                                                                                                tCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCa              >  2:80227/1‑141 (MQ=38)

                                                                                                                                tCTGAAGAGACCTGCGGTTCTTGTTATATAGAGCGTAAATACAATCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAAAATGTAGGTGATCTTGCTCCATGAGGTTAttaa              >  1:300952/1‑139 (MQ=11)

                                                                                                                                 ttGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg             <  2:196383/140‑1 (MQ=255)

                                                                                                                                 cTGAAGAGACCTAGGGTTCTTTTTATATTGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg             <  2:145131/141‑1 (MQ=14)

                                                                                                                                 cTGAAGAGACCTACGGTTCTTTTTATATAGAGCGGAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCGTGCTTACTTTAGTATGACGGTGATCTTGCTCAATGAGGTTATTCaa             >  2:161741/1‑140 (MQ=11)

                                                                                                                                  tGAAGAGACCTGCGGTTCTTTTTATATATAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTGTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa            <  1:202905/141‑1 (MQ=255)

                                                                                                                                  tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa            >  1:37486/1‑141 (MQ=38)

                                                                                                                                  tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa            <  2:116180/141‑1 (MQ=255)

                                                                                                                                   gAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat           <  1:106199/141‑1 (MQ=255)

                                                                                                                                    aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGTTAATCTTTCTCACTGAGGTTCTTCAAatt          >  1:151172/1‑140 (MQ=11)

                                                                                                                                    aaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCCATGAGGTTATTCAGata          >  2:321095/1‑141 (MQ=255)

                                                                                                                                     agagACCTACGGTTCTTTTTATATAGGGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGattt         >  2:200518/1‑139 (MQ=255)

                                                                                                                                      gagaCCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTGAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt        <  1:250356/141‑1 (MQ=38)

                                                                                                                                      gagaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt        <  2:180199/141‑1 (MQ=255)

                                                                                                                                          ccTGCGGTACTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGAATTCTTTGCTGTATTGATACTTTGATACCTTGTCTATCTTACTTTAATATGACGGTGATTTTGCTCAATGAGGTTATTCAGATATTTCGa    >  2:263896/1‑141 (MQ=255)

                                                                                                                                           cTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt   >  2:138293/1‑141 (MQ=255)

                                                                                                                                           cTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt   <  2:299331/141‑1 (MQ=38)

                                                                                                                                           cTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTAGTCTGGGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt   >  2:163566/1‑141 (MQ=255)

                                                                                                                                            tGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATg  >  1:352481/1‑141 (MQ=37)

TGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATG  >  CP000730/35799‑36079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`