Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,343,438 C→T 40.1% intergenic (+114/+250) USA300HOU_2200 → / ← alsD hypothetical protein/acetolactate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,343,4380CT40.1% 7.6 / 18.0 20intergenic (+114/+250)USA300HOU_2200/alsDhypothetical protein/acetolactate decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (7/5);  new base T (2/6);  total (9/11)
Fisher's exact test for biased strand distribution p-value = 1.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.66e-01

GATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATTA  >  CP000730/2343303‑2343575
                                                                                                                                       |                                                                                                                                         
gATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAg                                                                                                                                      <  2:48877/141‑1 (MQ=255)
 aTACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGt                                                                                                                                     <  2:132917/141‑1 (MQ=255)
  tACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTa                                                                                                                                    >  1:292200/1‑141 (MQ=255)
               tAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCCCCGATAAAGTGTCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAg                                                                                                                       <  1:276195/141‑1 (MQ=255)
                       aatTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTAcc                                                                                                               >  1:96960/1‑141 (MQ=255)
                               aaTAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTta                                                                                                       <  2:108522/141‑1 (MQ=255)
                                      ttATAGGTCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTa                                                                                                <  2:260503/141‑1 (MQ=255)
                                           gggCAAGCGCTGAAAAATAGGCGTATGTCTTTTTAGATATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGAAACAGATTAGCCTATACAATAGTAAATATTAAAAGAGTGTGTACCACAAAATATAATCTAAACaa                                                                                           >  2:27822/1‑140 (MQ=255)
                                                aGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAg                                                                                      <  1:44984/141‑1 (MQ=255)
                                                aGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAg                                                                                      >  1:340556/1‑141 (MQ=255)
                                                aGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAg                                                                                      >  1:91/1‑141 (MQ=255)
                                                    cTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTCTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCaaa                                                                                  >  1:159954/1‑141 (MQ=255)
                                                                  tttGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTgg                                                                    <  1:223530/141‑1 (MQ=255)
                                                                                 aTTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAAGATAACAGCAAATCTCGAATATCGGGTACCTAATAAATcac                                                     >  1:298279/1‑139 (MQ=255)
                                                                                   tGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCt                                                   <  1:126834/141‑1 (MQ=255)
                                                                                       cTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCact                                               >  2:252309/1‑141 (MQ=255)
                                                                                          gTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCactact                                            <  2:292200/141‑1 (MQ=255)
                                                                                            aaaTTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCTTCactattag                                          <  2:266640/141‑5 (MQ=255)
                                                                                                    gttgttTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTg                                  <  2:96960/141‑1 (MQ=255)
                                                                                                                           gATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAAAATAACAGCAACTCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTCATAGTGAAGGGATTTCTTTTATTACTTAg           >  2:280016/1‑141 (MQ=255)
                                                                                                                                    atatAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAgcagcaagtt  >  2:132799/1‑132 (MQ=255)
                                                                                                                                       |                                                                                                                                         
GATACAAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATTA  >  CP000730/2343303‑2343575

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: