mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.32.1 revision 5f2cb669173e
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | SNFR_21_63_0_S2394_L001_R1_001.good.fq | 359,460 | 50,646,524 | 100.0% | 140.9 bases | 141 bases | 94.5% |
| errors | SNFR_21_63_0_S2394_L001_R2_001.good.fq | 359,460 | 50,646,524 | 100.0% | 140.9 bases | 141 bases | 90.0% |
| total | 718,920 | 101,293,048 | 100.0% | 140.9 bases | 141 bases | 92.2% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | CP000730 | 2,872,915 | 32.2 | 2.4 | 100.0% | Staphylococcus aureus subsp. aureus USA300_TCH1516, complete |
| total | 2,872,915 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 1438 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 2 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 24 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.002 |
| reference sequence | pr(no read start) |
|---|---|
| CP000730 | 0.90902 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 0 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 6 |
| option | value |
|---|---|
| Mode | Full Polymorphism |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | OFF |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 2 |
| Polymorphism frequency cutoff | 0.025 |
| Polymorphism minimum variant coverage each strand | 2 |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | ≥3 bases |
| Skip base substitutions when they create a homopolymer flanked on each side by | ≥5 bases |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.5.0 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 20:45:07 22 Jun 2018 | 20:45:35 22 Jun 2018 | 28 seconds |
| Read alignment to reference genome | 20:45:35 22 Jun 2018 | 20:47:02 22 Jun 2018 | 1 minute 27 seconds |
| Preprocessing alignments for candidate junction identification | 20:47:02 22 Jun 2018 | 20:47:20 22 Jun 2018 | 18 seconds |
| Preliminary analysis of coverage distribution | 20:47:20 22 Jun 2018 | 20:47:46 22 Jun 2018 | 26 seconds |
| Identifying junction candidates | 20:47:46 22 Jun 2018 | 20:47:47 22 Jun 2018 | 1 second |
| Re-alignment to junction candidates | 20:47:47 22 Jun 2018 | 20:48:07 22 Jun 2018 | 20 seconds |
| Resolving best read alignments | 20:48:07 22 Jun 2018 | 20:48:37 22 Jun 2018 | 30 seconds |
| Creating BAM files | 20:48:37 22 Jun 2018 | 20:48:59 22 Jun 2018 | 22 seconds |
| Tabulating error counts | 20:48:59 22 Jun 2018 | 20:49:09 22 Jun 2018 | 10 seconds |
| Re-calibrating base error rates | 20:49:09 22 Jun 2018 | 20:49:10 22 Jun 2018 | 1 second |
| Examining read alignment evidence | 20:49:10 22 Jun 2018 | 20:55:02 22 Jun 2018 | 5 minutes 52 seconds |
| Polymorphism statistics | 20:55:02 22 Jun 2018 | 20:55:05 22 Jun 2018 | 3 seconds |
| Output | 20:55:05 22 Jun 2018 | 20:55:13 22 Jun 2018 | 8 seconds |
| Total | 10 minutes 6 seconds | ||
