| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 19,009 | C→T | 100% | S222F (TCC→TTC) | USA300HOU_0014 → | DHH subfamily phosphodiesterase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 19,009 | 0 | C | T | 100.0% | 52.3 / NA | 20 | S222F (TCC→TTC) | USA300HOU_0014 | DHH subfamily phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (7/13); total (7/13) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TTTAGATAACTACGATGAGATTACGCAAAATATGAATGATACGCAGCGTTCGGAAATCAACTCAATGGTAACGCGTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTCCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAGTGTTGGTTATCGTGCCCAATTAACATTAAGTATCGGT > CP000730/18881‑19136 | tttAGATAACTACGATGAGATTACGCAAAATATGAATGATACGCAGCGTTCGGCAATCAACTCAATGGTAACGCGTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAATTTCGATGCATTCGt > 1:145074/1‑141 (MQ=255)tttAGATAACTACGATGAGATTACGCAAAATATGAATGATACGCAGCGTTCGGAAATCAACTCAATGGTAACGCGTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGt > 1:4477/1‑141 (MQ=255) cAAAATATGAATGATACGCAGCGTTCGGAAATCAACTCAATGGTAACGCGTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTa > 2:46939/1‑141 (MQ=255) aaTATGAATGATACGCAGCGTTCGGAAATCAACTCAATGGTAACGCGTGGCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCt < 1:169100/141‑1 (MQ=255) tgaatgaTACGCAGCGTTCGGAAATCAACTCAATGGTAACGCGTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACt < 1:159665/141‑1 (MQ=255) aatgaTACGCAGCGTTCGGAAATCAACTCAATGGTAACGCGTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTa < 1:212049/141‑1 (MQ=255) aatgaTACGCAGCGTTCGGAAATCAACTCAATGGTAACGCGTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTa < 1:211995/141‑1 (MQ=255) aCGCAGCGTTCGGAAATCAACTCAATGGTAACGCGTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTagcagca > 1:111903/1‑139 (MQ=255) cGGAAATCAACTCAATGGTAACGCTTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTAGTAGCCTATTTAAATCAGAAAATATTAGCTGACTTAGAAGAATCTAAATTTg < 1:59038/141‑1 (MQ=255) aTGGTAACGCGTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCaa > 1:60871/1‑141 (MQ=255) aTGCTAACGCTTGTCACTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCTATCAATTCGTAGCCGATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCaa < 2:167907/141‑1 (MQ=255) gtCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGaaaaa < 1:1868/141‑1 (MQ=255) aTTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGCAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAgt < 1:161174/141‑1 (MQ=255) cGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAGTGTTGGt < 1:132598/141‑1 (MQ=255) gATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAGTGTTGGtt < 2:78118/141‑1 (MQ=255) ggCAACTGAGTATAATATGTTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAGTGTTGGTTATCg < 1:242547/141‑1 (MQ=255) gCAACTGAGTATAATATATTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTAAATTACGTGAAAAAAGTGTTGGTTATCGt > 1:90253/1‑141 (MQ=255) tatatTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAGTGTTGGTTATCGTGCCCAATTAACAtt > 2:81687/1‑141 (MQ=255) atTTTTCAAAAGATACAGTTTCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAGTGTTGGTTATCGTGCCCAATTAACATTAAg < 1:46939/141‑1 (MQ=255) aaaaGCTACATTTTCGATCGATTCGTAGGCTAATTAAATCAAAAAATATTTGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAACATTGTTGGTTATCGTGCCCAATTAACATTAAGTATCGgt < 2:208341/141‑1 (MQ=255) | TTTAGATAACTACGATGAGATTACGCAAAATATGAATGATACGCAGCGTTCGGAAATCAACTCAATGGTAACGCGTGTCATTAGTCGATGGGCAACTGAGTATAATATATTTTTCAAAAGATACAGTTCCGATCAATTCGTAGCCTATTTAAATCAAAAAATATTAGCTGACTTAGAAGAATCTAAATTTGATATCTTGAGTCAATTACGTGAAAAAAGTGTTGGTTATCGTGCCCAATTAACATTAAGTATCGGT > CP000730/18881‑19136 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |