| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 981,004 | G→A | 15.7% | G213S (GGT→AGT) | leuA1 → | 2‑isopropylmalate synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 981,004 | 0 | G | A | 15.7% | 53.3 / 4.3 | 26 | G213S (GGT→AGT) | leuA1 | 2‑isopropylmalate synthase |
| Reads supporting (aligned to +/- strand): ref base G (10/10); new base A (2/2); total (13/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
AGGATGTTCGACACCATTAGAATACGGAGATATTTTTAATTACTTTGTAAAAAAATATTCTAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAATAGAAAGTACGGAATTCACTTTACCCGACGTATATAAAACT > CP000730/980868‑981141 | aGGATGTTCGACACCATTAGAATACGGAGATATTTTTAATTACTTTGTAAAAAAATATTCTAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATTGTgc > 1:64511/1‑141 (MQ=255) ttCGACACCATTAGAATACGGAGATATTTTTAATTACTTTGTAAAAAAATATTCTAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTgcaaagca > 2:208992/1‑141 (MQ=255) ttCGACACCATTAGAATACGGAGATATTTTTAATTACTTTGTAAAAAAATATTCTAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATAGTgcaaagca > 1:85450/1‑141 (MQ=255) ttCGACACCATTAGAATACGGAGATATTTTTAATTACTTTGTAAAAAAATATTCTAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATAGTgcaaagca > 1:33874/1‑141 (MQ=255) ttAGAATACGGAGATATTTTTAATTACTTTGTAAAAAAATATTCTAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACt < 2:158938/141‑1 (MQ=255) ggAGATATTTTTAATTACTTTGTAAAAAAATATTCTAACATAATGTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTg < 2:224063/141‑1 (MQ=255) agaTATTTTTAATTACTTTGTAAAAACATATTCTAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATATTTgaa > 2:150302/1‑140 (MQ=255) tAATTACTTTGTAAAAAAATATTCTAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGCGAATTGGTga > 1:165423/1‑141 (MQ=255) cTTTGTAAAAAAATATTCTAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTCCATTTTTGAGAATTGGTGAGAGAGc > 1:9109/1‑141 (MQ=255) aaaaATTCTAACATATTTTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCt < 2:234549/141‑1 (MQ=255) aaaTATTATCACATAATTTTTTCTGCGCATTGTCATCCCGGTCTATGCTGGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGTGAATTGGTGAGAGAGCGGGTAATGCt < 2:149591/141‑1 (MQ=255) aaTATTCTAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTc < 2:79572/141‑1 (MQ=255) aaCATAATTTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTGCATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAg < 2:133641/141‑1 (MQ=255) tttttCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAaataat < 1:13662/141‑1 (MQ=255) ttttCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTATCTGCAATTTTAAATAGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAAtt < 2:85450/141‑1 (MQ=255) ttttCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATAGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAAtt < 2:33874/141‑1 (MQ=255) aTTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGCGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTgg > 2:181079/1‑140 (MQ=255) tAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACaaaaaa > 2:217022/1‑141 (MQ=255) cTGCAAATACATTAGCTGCAATTTTAAATTGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAATAGAAAGTACGGAAt < 2:64511/141‑1 (MQ=255) cTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAATAGAAAGTACGGAAt > 2:11871/1‑141 (MQ=255) actACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAATAGAAAGTACGGAATTCACt < 1:229100/139‑1 (MQ=255) aaCATTAGCTGCAATTTGAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAATAGAAAGTACGGAATTCACttt < 1:206639/140‑1 (MQ=255) tAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAATAGAAAGTACGGAATTCACTTTACCCg > 1:185521/1‑141 (MQ=255) tttAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAGTGATCCTATTGAGGAAATAAGTACTGTTTTGACAAGACACCAAATAGAGAGGACGGAATTAACTTTagacaaagtataaaa > 2:69082/1‑127 (MQ=255) ttAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAATAGAAAGTACGGCATTCAATTTACCCGACGTA‑ATaaaa > 2:134136/1‑141 (MQ=255) aaTGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAATAGAAAGTACGGAATTCACTTTACCCGACGTATATAAAACt < 1:11871/141‑1 (MQ=255) | AGGATGTTCGACACCATTAGAATACGGAGATATTTTTAATTACTTTGTAAAAAAATATTCTAACATAATTTTTTCTGCTCATTGTCATAACGATCTAGGGTTGGCTACTGCAAATACATTAGCTGCAATTTTAAATGGTGCAAAGCAAATAGAAACTACATTTTTGAGAATTGGTGAGAGAGCGGGTAATGCTCCTATTGAGGAAATAATTACTATTTTGACAAAAAAACAAATAGAAAGTACGGAATTCACTTTACCCGACGTATATAAAACT > CP000730/980868‑981141 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |