Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,337,959 C→T 100% intergenic (‑125/‑119) USA300HOU_2195 ← / → USA300HOU_2196 possible MerR family transcriptional regulator/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,337,9590CT100.0% 95.0 / ‑6.5 32intergenic (‑125/‑119)USA300HOU_2195/USA300HOU_2196possible MerR family transcriptional regulator/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (22/9);  minor base A (1/0);  total (23/9)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TTTAGTTTTCATTGCGACTATCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTACATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTGGGTGATGCGGAAAATTTG  >  CP000730/2337823‑2338091
                                                                                                                                        |                                                                                                                                    
tttAGTTTTCATTGCGACTATCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATAttt                                                                                                                                  >  2:15202/1‑141 (MQ=255)
      tttCATTGCGACTATCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGt                                                                                                                            >  2:31396/1‑141 (MQ=255)
        tCATTGCGACTATCATTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTATTATACATTCACGCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGttt                                                                                                                          >  1:130712/1‑141 (MQ=255)
         cATTGCGACTATCCTTTCAGCACAGTGTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGtttt                                                                                                                         >  1:247417/1‑141 (MQ=255)
             gCGACTATCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTGATATTTCATCGTTTTTAAt                                                                                                                     >  1:9151/1‑141 (MQ=255)
              cGACTATCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAAtt                                                                                                                    >  1:37114/1‑141 (MQ=255)
              cGACTATCCTTTCAGATATGTTTGGTCGTCTAAAGTAATGTTGATTTATATATTGTCATCTTCGTTTGAATACTTCTTGTTTTATTACTCATATTTAAATTTGTCTCTTTTTTAACATTTTATCTTTCATCGTTTTTAttt                                                                                                                    >  2:94153/1‑141 (MQ=255)
              cGACTATCCTGTCAGTTATGTTGGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCGTCGTTTGAATACTTCTTATTTTCATAATCAAAATTAAATGTAGCTCTTCTTTTACCTTTTCAAATTCATTGTTTTTTAtt                                                                                                                    >  2:38737/1‑141 (MQ=255)
                 cTATCCTTTCATTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTCCTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACt                                                                                                                 <  1:36870/141‑1 (MQ=255)
                 cTATCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACt                                                                                                                 <  1:33737/141‑1 (MQ=255)
                              tATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATCTTTCATCGTTTTTAATTACTTTAACAATTGtat                                                                                                    >  2:50402/1‑141 (MQ=255)
                                   ttGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACtt                                                                                               >  1:118572/1‑141 (MQ=255)
                                    tGGTCGTCTAAAGTAATGTTGCTTAATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTa                                                                                              >  1:159551/1‑141 (MQ=255)
                                       tcgtcTAAAGTAATGTTTCTTTATATATTGTAATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAat                                                                                           <  2:39621/141‑1 (MQ=255)
                                             aaaGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTaa                                                                                     >  2:138768/1‑141 (MQ=255)
                                                       tGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATaa                                                                           >  2:40583/1‑141 (MQ=255)
                                                              tatatTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTaa                                                                    >  1:104219/1‑141 (MQ=255)
                                                                      cATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGata                                                            >  2:88899/1‑141 (MQ=255)
                                                                         tttCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGataata                                                         <  2:169178/140‑1 (MQ=255)
                                                                         cTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGataata                                                         <  1:72304/141‑1 (MQ=255)
                                                                             gTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTa                                                     >  2:246607/1‑141 (MQ=255)
                                                                                   aTACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTATTATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTTACATTGATAATATTTAAttttt                                               >  2:119261/1‑141 (MQ=255)
                                                                                   aTACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAAttttt                                               >  2:133104/1‑141 (MQ=255)
                                                                                      cttcttATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGa                                            >  1:216916/1‑141 (MQ=255)
                                                                                                 attaCTCAAATTTAAATTTGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTa                                 <  2:159551/141‑1 (MQ=255)
                                                                                                             taaatttGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTTGTTGCAAttttt                     >  1:215816/1‑141 (MQ=255)
                                                                                                                   tGTCTCTTTTTTAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTGGGTGa               <  1:88899/141‑1 (MQ=255)
                                                                                                                          tttttAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTGGGTGATGCGGaa        <  1:18769/141‑1 (MQ=255)
                                                                                                                             ttAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTCTTTTAGTTGCAATTTTTGGGTGATGCGGaaatt     >  2:47057/1‑139 (MQ=255)
                                                                                                                              tAACATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTGGGTGATGCGGAAAAtt    >  2:70298/1‑141 (MQ=255)
                                                                                                                                aCATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTGGGTGATGCGGAAAATTTg  <  2:216916/141‑1 (MQ=255)
                                                                                                                                aCATTTTATATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTGGGTGATGCGGAAAATTTg  >  1:196159/1‑141 (MQ=255)
                                                                                                                                        |                                                                                                                                    
TTTAGTTTTCATTGCGACTATCCTTTCAGTTATGTTTGGTCGTCTAAAGTAATGTTGCTTTATATATTGTCATCTTCGTTTGAATACTTCTTATTTTATTACTCAAATTTAAATTTGTCTCTTTTTTAACATTTTACATTTCATCGTTTTTAATTACTTTAAAAATTGTATAACTTAAATATTTAAAATGATATAATCACTAAGATTGATAATATTTAATTTTTTGAAAATTATTTTAGTTGCAATTTTTGGGTGATGCGGAAAATTTG  >  CP000730/2337823‑2338091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑