| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | CP000730 | 2,361,729 | G→A | 50.3% | P193S (CCA→TCA) | secY1 ← | Sec family Type I general secretory pathway preprotein translocase SecY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP000730 | 2,361,729 | 0 | G | A | 50.3% | ‑1.9 / 35.7 | 30 | P193S (CCA→TCA) | secY1 | Sec family Type I general secretory pathway preprotein translocase SecY |
| Reads supporting (aligned to +/- strand): ref base G (6/9); new base A (9/6); total (15/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01 | |||||||||||
ACGAACGGCTTCAAGTACATAAATAGCACCTACAGTTAATAGTATCAGTGACACTAAAAGTCCTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATCACCAAGCCATATTAAGAAAGCAGTTCCTGCTGTCAAAACTAGTGCTATTAATAAATAACTCAT > CP000730/2361589‑2361864 | cccaaCGCCTTCTAGAAGATTAATAGTACCTACAGTTAATAGTATCAGTGACACTAAAAGTCCTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACTCAGTGTGCCCAAATTGAATTAGActggctgg < 1:172541/138‑1 (MQ=255) taaaGTACATAAATAGCACCTACCGGTAATAGTATCAGTGACACTAAAAGTCCTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGACAACGTTGAt < 2:19309/139‑1 (MQ=255) aaTAGCACCTACAGTTAATAGTATCAGTGACACTAAAAGTCCTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCGc > 1:156945/1‑141 (MQ=255) aaTAGCACCTACAGTTAATAGTATCAGTGACACTAAAAGTCCTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGACAACGTTGATAAAATACCCGc > 2:36378/1‑141 (MQ=255) gCACCTAACGTTAATAGTATCAGTGACACTAAAAGTCCTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCGCAAat < 1:201402/141‑1 (MQ=255) tACAGTTAATAGTATCAGTGACACTAAAAGACCTAATACTTTCAACCATGCTAATGAGGTGTCTTCTTGCCCAAAGAAAGCAGATTGTCCAAATTGAATTAGACTGGCTGACAACGATGATAAAATACCCGCAAATAAGaa > 2:86876/1‑140 (MQ=255) gTTAATAGTATCAGTGACACTAAAAGTCCTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGACAACGTTGATAAAATCCCCGCAAATATGatcata > 1:155369/1‑141 (MQ=255) ttAATAGTATCAGTGACACTAAAAGTCCTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCGCAAATATGATAATAg > 2:139122/1‑141 (MQ=255) tAATAGTATCAGTGACACTAAAAGTCCTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGACAACGTTGATAAAATACCCGCAAATATGataa > 1:23197/1‑137 (MQ=255) tAATAGTATCAGTGACACTAAAAGTCCTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGACAACGTTGATAAAATACCCGCAAATATGataa < 2:23197/137‑1 (MQ=255) aaTAGTATCAGGGACACTAAAAGTCCTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCGCAAATATGATAATAGaa < 1:96123/141‑1 (MQ=255) aTAGTATCAGTGACACTAAAAGTCCTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCGCAAATATGATAATAGaaa < 2:83136/141‑1 (MQ=255) tGACACTAAAAGTCCTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTAcc < 1:153542/141‑1 (MQ=255) ccTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTGTGGCCAAATTGAATTAGACTGGCTGACAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGa < 1:47064/141‑1 (MQ=255) ttCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCGCAAATATGATATTAGAAATACCATTACCAACACCGTACTGAGTGATTTGa < 1:139122/141‑1 (MQ=255) ttCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGa > 1:26781/1‑141 (MQ=255) ttCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCACAAATATGATAATAGATGAAAAAGGACGCAAACCGAACTGAGTGATTTGa > 2:226444/1‑141 (MQ=255) aaCCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGACAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATcc > 2:189095/1‑140 (MQ=255) aCCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGACAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATCAc > 2:71396/1‑141 (MQ=255) cATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATCACCa > 2:155212/1‑141 (MQ=255) aTGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGACAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATCACCaa > 1:226333/1‑141 (MQ=255) aTGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGACAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATCACCaa > 1:226340/1‑141 (MQ=255) tGCTAATGATGTATCGTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGACAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATCACCAAg > 1:185570/1‑141 (MQ=255) tcttctTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATCACCAAGCCATCTTAAGaaa > 1:136602/1‑141 (MQ=255) tcttGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATCACCAAGCCATATTAAGAAAGCa < 1:212187/141‑1 (MQ=255) gCCCAACGAACGCCGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCGCAAATATGATAATAGAAATCCCATTACCAACACCGAACTGAGTGATTTGATCACCAAGCCATATTAAGAAAGCAGTTc < 2:129983/141‑1 (MQ=255) cAACGAACGCAGTTTGGCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATATCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATCACCAAGCCATATTAAGAAAGCAGTTCctg < 2:145665/141‑1 (MQ=255) gCAGTTTGTCCAAATTGCATTAGTCTTGCTGACAACGTTGATAAAATACCCTCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATCACCAAGCCATATTAAGAAAGCAGTTCCTGCTGTCaaa < 2:170273/141‑1 (MQ=255) aaTTGAATTAGACTGGCTGACAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATCACCAAGCCATATTAAGAAAGCAGTTCCTGCTGTCAAAACTAGTGCTAtt < 2:185570/141‑1 (MQ=255) aaTTGAATTAGACTGGCTGACAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATCACCAAGCCATATTAAGAAAGCAGTTCCTGCTGTCAAAACTAGTGCTAtt < 1:103834/141‑1 (MQ=255) aCTGGCTCGCAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACAATTACCAACACCGAACTGAGTGATTTGATCACCAAGCCATATTAAGAAAGCAGTTCCTGCTGTCAAAACTAGTGCTATTAATAAATAACt < 2:2976/141‑1 (MQ=255) ggctggcCACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATCACCAAACCATATTAAGAAAGCAGTTCCTGCTGTCAAAACTAGTACTATTAATAAATAACTCAt > 2:246904/1‑141 (MQ=255) | ACGAACGGCTTCAAGTACATAAATAGCACCTACAGTTAATAGTATCAGTGACACTAAAAGTCCTAATACTTTCAACCATGCTAATGATGTATCTTCTTGCCCAACGAACGCAGTTTGTCCAAATTGAATTAGACTGGCTGGCAACGTTGATAAAATACCCGCAAATATGATAATAGAAATACCATTACCAACACCGAACTGAGTGATTTGATCACCAAGCCATATTAAGAAAGCAGTTCCTGCTGTCAAAACTAGTGCTATTAATAAATAACTCAT > CP000730/2361589‑2361864 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |