Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 522,654 C→T 100% T169I (ACA→ATA)  USA300HOU_0484 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730522,6540CT83.5% 38.7 / 2.3 20T169I (ACA→ATA) USA300HOU_0484hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (4/12);  total (6/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGATTTTAGAAAATTAGTAAAAATATTAGCGCAACATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTACATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCATGTGGTCGTTTGATGTGTTGTTTAAAATATGAAAATGACTATTATGAG  >  CP000730/522520‑522787
                                                                                                                                      |                                                                                                                                     
tGATTTTAGAAAATCAGTAAAAATATTAGCGCAACATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATAttttt                                                                                                                                 <  1:207984/141‑1 (MQ=255)
      tAGAAAATTAGTAAAAATATTAGCGCAACCTTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCCAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGa                                                                                                                           <  1:52787/141‑1 (MQ=255)
        gAAAATTAGTAAAAATATTAGCGCAACATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGAtt                                                                                                                         <  1:204756/141‑1 (MQ=255)
               aGTAAAAATATTAGCGCAACATTTAAAAACACGGATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTACATTTTTAGGGGATTTTGAAcc                                                                                                                  <  2:120948/141‑1 (MQ=255)
                     actattctcggaaCATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATc                                                                                                            <  1:107045/130‑1 (MQ=255)
                            gcgcAACATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGGTTAAg                                                                                                     >  2:39436/1‑141 (MQ=255)
                                    tttAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTAAATTTTTAGGGGCGTTTGAACCAGTATCGATTAAGAGGGCTaa                                                                                             >  1:126459/1‑141 (MQ=255)
                                     ttAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATGAAGAAGGCTAAg                                                                                            >  2:77748/1‑141 (MQ=255)
                                                               aaTTGGGGTAAGGGAGGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAAc                                                                  <  1:72520/141‑1 (MQ=255)
                                                                 ttGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTa                                                                <  2:58173/141‑1 (MQ=255)
                                                                   ggTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGTACCTTGTGGTAGGTCATTATGTTGTTCTATATTTTTAGGAGAATTTGTACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTaac                                                              <  2:66161/141‑2 (MQ=255)
                                                                            gATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTACATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGt                                                     >  1:184687/1‑141 (MQ=255)
                                                                                aaGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTTGTTATGTTGTTCTACATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCAt                                                 <  2:248785/141‑1 (MQ=255)
                                                                                  gCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGGTATGTTGGTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCAtgt                                               <  1:219913/141‑1 (MQ=255)
                                                                                    cAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCATGTgg                                             <  1:249888/141‑1 (MQ=255)
                                                                                        ttGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCATGTGGTCGt                                         >  1:161795/1‑141 (MQ=255)
                                                                                                            ttGTGGTAGGTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCATGTGGTCGTTTGATGTGTTGTTTAAAata                     <  2:36858/141‑1 (MQ=255)
                                                                                                                     gTCGTTATGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAACATTTCTGGTGCATGTGGTCGTTTGATGTGTTGTTTAAAATATGAAAATGa            <  1:131450/141‑1 (MQ=255)
                                                                                                                         ttAGGTTGTTCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCATGTGGTCGTTTGATGTGTTGTTTAAAATATGAAAATGACtat        <  2:37428/141‑1 (MQ=255)
                                                                                                                               tgttCTATATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCATGTGGTCGTTTGATGTGTTGTTTAAAATATGAAAATGACTATTATGAg  >  1:28983/1‑141 (MQ=255)
                                                                                                                                      |                                                                                                                                     
TGATTTTAGAAAATTAGTAAAAATATTAGCGCAACATTTAAAAACACGTATCGAGTTGAGACAAATTGGTGTAAGGGATGAAGCCAAATTGCTTGGCGGTATCGGACCTTGTGGTAGGTCGTTATGTTGTTCTACATTTTTAGGGGATTTTGAACCAGTATCGATTAAGATGGCTAAGGATCAAAATTTATCATTAAATCCAACTAAAATTTCTGGTGCATGTGGTCGTTTGATGTGTTGTTTAAAATATGAAAATGACTATTATGAG  >  CP000730/522520‑522787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: