Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 584,121 G→T 100% intergenic (+205/‑67) rplA → / → rplJ ribosomal protein L1/ribosomal protein L10

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730584,1210GT100.0% 54.6 / NA 20intergenic (+205/‑67)rplA/rplJribosomal protein L1/ribosomal protein L10
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/5);  total (15/5)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

AGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGG  >  CP000730/583983‑584239
                                                                                                                                          |                                                                                                                      
aGTAATTTCATAGATTTATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTg                                                                                                                      >  2:116179/1‑141 (MQ=255)
         cAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAg                                                                                                             <  1:156119/141‑1 (MQ=255)
                           gtgATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAAttt                                                                                           >  2:151279/1‑141 (MQ=255)
                            tgATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTTTTTTTTTTTTAAAGTGCTTTTTTTATTAAtttt                                                                                          >  2:168126/1‑141 (MQ=255)
                            tgATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAAtttt                                                                                          >  1:216156/1‑141 (MQ=255)
                                            gACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCaaaa                                                                          >  1:121783/1‑141 (MQ=255)
                                                 tAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTa                                                                     >  1:74431/1‑141 (MQ=255)
                                                             ataACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTc                                                         >  1:237460/1‑141 (MQ=255)
                                                                  ttAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAAt                                                    <  2:11678/141‑1 (MQ=255)
                                                                        tttATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCCCCAAAAATTTAAATGGAGGCGTCTGAATGTCTGc                                              >  1:211130/1‑141 (MQ=255)
                                                                         ttATCCTGCCGAGTCTAAAATTGACTTGAACGTTATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCt                                             <  1:151459/141‑1 (MQ=255)
                                                                         ttATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTGTTTTTATTAATTTTAAAAAAAGCACCAAAAAATTAAATGGAGGTGTCTAAATGTCTGcg                                             >  2:135175/1‑140 (MQ=255)
                                                                          tATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGGGTCTGAATGTCTGCTa                                            >  1:100096/1‑141 (MQ=255)
                                                                           aTCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTAt                                           >  1:108082/1‑141 (MQ=255)
                                                                            tCCTGCCGAGGCTAAAATTGACTTGAACGTGATGAACTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGcaacg                                          >  2:1845/1‑137 (MQ=255)
                                                                                gCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTg                                      >  1:134493/1‑141 (MQ=255)
                                                                                                aCTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACt                      >  1:138127/1‑141 (MQ=255)
                                                                                                 cTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTAACTTAGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTa                     <  2:216156/141‑1 (MQ=255)
                                                                                                       cGTGATGATCTATGATCTTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTtgat               <  2:152292/141‑1 (MQ=255)
                                                                                                                    gatctTTCAAGCACTTTTTGCCTTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACAAAACATTTCAATGGAGGTCTCGGCATGTCTGCTAACACTGCAGCTAAAAAACAACAAATTGATGCAATtgatgacc  >  2:116702/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                      
AGTAATTTCCAAGTTATATTACTTATTGTGATTATTTTACCTAAGACAGTAGGAGTTATTTATAACTTAAAATTTATCCTGCCGAGGCTAAAATTGACTTGAACGTGATGATCTATGATCTTTCAAGCACTTTTTGCCGTGGGTAGAAAGTGCTTTTTTTATTAATTTTAAAAAAAGCACCAAAAATTTAAATGGAGGTGTCTGAATGTCTGCTATCATTGAAGCTAAAAAACAACTAGTTGATGAAATTGCTGAGG  >  CP000730/583983‑584239

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: