BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,315	G→A	59.1%	Y79Y (TAC→TAT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,315	0	G	A	59.1%	351.3 / inf	857	Y79Y (TAC→TAT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (169/173); new base A (227/271); total (405/452)
Fisher's exact test for biased strand distribution p-value = 2.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 862 total aligned reads displayed.

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTC  >  CP000730/36175‑36455

ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATg                                                                                                                                              <  1:609937/141‑1 (MQ=32)

    cAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGATTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCACTGGCACGATATAAATAGCTGCATTTTCCTTTTATTTTGATGTTCg                                                                                                                                          >  2:630589/1‑141 (MQ=255)

    cAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTGCCTCTGCATCAATGTGACGATATACATAGCTCCATTTTCCTTTTATTTTGCTGTACg                                                                                                                                          >  2:71589/1‑141 (MQ=11)

      aatggttccaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                                                                        <  1:245139/132‑1 (MQ=11)

      aaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGtc                                                                                                                                        <  1:780735/141‑1 (MQ=12)

       aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTTCGtct                                                                                                                                       >  1:93430/1‑141 (MQ=17)

       aTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACAtct                                                                                                                                       >  2:642955/1‑141 (MQ=17)

        ttGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATCTAAATAGCTCCATTTTCCTTTTATTTTGATTTACCtctc                                                                                                                                      >  2:304243/1‑141 (MQ=12)

         tGTTTAATGAGAGGTGTGAGAAACGCATAGGCTGAATGATGAGCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCAGCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtca                                                                                                                                     <  2:738528/141‑1 (MQ=11)

             tAATGACACGTTTGATAATCGCTTATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCaa                                                                                                                                 <  1:70983/141‑1 (MQ=11)

              aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                                                                >  1:59313/1‑141 (MQ=17)

              aaTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                                                                <  1:839674/141‑1 (MQ=12)

              aaTGAGACGTGTTAAAAACGCATAGGCTGAACGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAAt                                                                                                                                <  1:21318/141‑1 (MQ=11)

               aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCGTTTTCCTTTTATTTTGATATACGTCTCATAAATa                                                                                                                               >  2:833169/1‑141 (MQ=255)

               aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATa                                                                                                                               >  2:704584/1‑141 (MQ=17)

               aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATa                                                                                                                               <  1:634987/141‑1 (MQ=32)

               aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTCCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATa                                                                                                                               <  2:324753/141‑1 (MQ=12)

               aTGAGAATTTTGAGTATCGTATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATa                                                                                                                               <  1:355579/141‑1 (MQ=11)

                tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATTTCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAc                                                                                                                              <  1:330038/141‑1 (MQ=17)

                ggataCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAc                                                                                                                              <  2:139082/137‑1 (MQ=12)

                 aaaacatttGACAAACGCATATGCTGAATGATTATCTCGTTCCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACg                                                                                                                             <  1:304487/135‑1 (MQ=11)

                        ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTg                                                                                                                      >  1:761511/1‑141 (MQ=17)

                        ttGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTAGGTCTCGTCGATACGCCAttag                                                                                                                      >  1:475105/1‑139 (MQ=12)

                                cGCACATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAACAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGc                                                                                                              <  1:505629/141‑1 (MQ=255)

                                  cATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCtt                                                                                                            <  2:21075/141‑1 (MQ=35)

                                    tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGGATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAACCtttt                                                                                                          >  2:276641/1‑141 (MQ=255)

                                       gCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTa                                                                                                       >  2:160748/1‑141 (MQ=25)

                                        cTGAATGATTATTCCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                                                                      <  2:261545/141‑1 (MQ=255)

                                        cTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAt                                                                                                      >  1:810050/1‑141 (MQ=38)

                                         tGAATGATTATCTCGTTGCTTACGCAACAAAATATCTTATGTATGTCCCTCTGTATCAATGGCACTATATAAATAGCTCCATTTTCCTTTTATTTTGATATTCGTCTCATAAATACGCCATTTGTGATAGACGTTTGTATg                                                                                                     >  1:445661/1‑141 (MQ=11)

                                              gcttacttcttcgCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCtttt                                                                                                <  2:788661/129‑1 (MQ=14)

                                               aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCATTACGCCATTTTTAATAAGCTTTTTTATGCttttt                                                                                               >  2:290488/1‑141 (MQ=255)

                                               aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTTATCAATACGCCATTTGTAATAGACTTTTTTTATCttttt                                                                                               >  1:266703/1‑141 (MQ=11)

                                               aTTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTACCCttttt                                                                                               >  2:125845/1‑141 (MQ=255)

                                               aTTATCTCGTTGCTTAAGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCCCCATTTTCCTTTTATTTTGGTATACGTCTCATCAATACGCCAATAGTAATAAGCTTTATTATCtttttt                                                                                               >  1:417508/1‑141 (MQ=11)

                                                ttATCTCGTTGCTTACGCAATCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttc                                                                                              <  1:249966/141‑1 (MQ=255)

                                                ttATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTTATGTTCTTCTCTTCAATACGCCGTTTTTACTTAGCTTTTTTTTGCtttttt                                                                                              >  1:22411/1‑140 (MQ=11)

                                                ttATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACACCACTAGAAATAAGCTTTTGAATGCtgtata                                                                                              >  1:29390/1‑136 (MQ=11)

                                                 tATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttct                                                                                             <  1:533147/141‑1 (MQ=40)

                                                  ttctcGTTGCTTACGCAACCAAGTATTTAATGTATGTCCCTCTGTATCAATGCCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                            <  2:81684/140‑1 (MQ=14)

                                                  tgctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctt                                                                                            <  2:371182/139‑1 (MQ=255)

                                                  aTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGTTATAAATAGCTCCTTTTTCCTTTTATTTTGATATACGTCTCATCAATATGCCATTTGTTATAAGCTTTTTTATGCTTTttctt                                                                                            >  1:553071/1‑141 (MQ=14)

                                                    ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttctttc                                                                                          >  2:771946/1‑139 (MQ=255)

                                                    ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                                                                          <  2:665852/141‑1 (MQ=255)

                                                    ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGTCACGATATAACTAGCTCCATTTTCCTTTTATTTTGCTGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTCTTATGCTTTTTCTTcc                                                                                          >  2:549397/1‑141 (MQ=255)

                                                            ttACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGa                                                                                  <  1:68267/141‑1 (MQ=255)

                                                             tACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGTCGTTTGTAATAAAGTTTTTTATGCTTTTTCTTTCAAttttttt                                                                                 >  2:336423/1‑138 (MQ=11)

                                                                gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                                                              <  1:74814/141‑3 (MQ=255)

                                                                gCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                                                              <  1:128873/141‑3 (MQ=255)

                                                                  aagcatatctctgctgtatgtCCCTCGGTAGCTATGGTACGATATAAATATCTCCATGTGCGTTTTATTTTGATATACGTCTTATACATACGCCATTTGTAATAAGCTTTTTTATGCTTGTTCGTCGAAATTTGATATaaa                                                                            <  2:683479/127‑1 (MQ=255)

                                                                   aCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTGAAATaaaa                                                                           >  2:486666/1‑141 (MQ=11)

                                                                   aCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTAGTAATAAGCTTTTTTATGCTTTTTATTCCAAATTTGATATaaaa                                                                           <  1:458922/141‑1 (MQ=11)

                                                                    ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                          >  2:651204/1‑141 (MQ=38)

                                                                     cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTTCTTTTTTTTTTTTATACGTCTCATCAATACTCCATTTGTAATATGCTTTTTTATTCTTTTTCTTCCAATTTTGATATAAAAtt                                                                         >  1:695805/1‑141 (MQ=11)

                                                                     cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAtt                                                                         >  1:664464/1‑141 (MQ=255)

                                                                       aaTATCTAATGTATGTCCCTCTGTAGCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                       <  1:829096/141‑1 (MQ=255)

                                                                         tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTAATTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                     <  1:819935/141‑1 (MQ=11)

                                                                             tAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                                 <  2:748651/141‑1 (MQ=255)

                                                                             tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGTTGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTTGGGCata                                                                 >  1:372456/1‑141 (MQ=255)

                                                                              aaTGTAGGGCCCTCTGTATCAAGGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCatat                                                                <  2:529219/141‑1 (MQ=11)

                                                                               atgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                                                               >  2:418121/1‑141 (MQ=255)

                                                                                   ttttcctgctGCATCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATACATTTGGGGCATATTCTTg                                                           <  2:457263/133‑1 (MQ=11)

                                                                                   ttctCCCTCTGCATCAATGGCACGATATAAATTGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCGAGTGTGATATAAAATTGGGGCATATTCTTg                                                           <  2:348139/138‑1 (MQ=11)

                                                                                   atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                           >  2:389491/1‑141 (MQ=255)

                                                                                    tgtTCCTCTGTAACAATGGCAAGATATAAATAACTCCATTTTCCTTTTAGTTTGATATACGTAACATCAATACGCTATTTGGAATAAGCTTTTTTATGCTTTGTCTTCCAAATTGGATATAAAATTGGGGCATATTCTTGa                                                          <  2:72766/141‑1 (MQ=11)

                                                                                      tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                        >  2:18692/1‑141 (MQ=18)

                                                                                         ctctGTATCAATGGCACGATATAAATAGCTTCATTTTCTCTTTACTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCa                                                     <  1:645655/141‑1 (MQ=11)

                                                                                          tctGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATTTTCTTGAACCCaa                                                    >  2:90141/1‑141 (MQ=255)

                                                                                            tgtagcAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACg                                                  <  1:345464/136‑1 (MQ=255)

                                                                                            tgtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACg                                                  >  1:385147/4‑141 (MQ=255)

                                                                                              taTCAATGGCACGATATAAATAGATCCAGTTTCCTTGTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTGTGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAATCCAACGGt                                                >  2:405129/2‑141 (MQ=11)

                                                                                                tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                              <  1:797033/141‑1 (MQ=21)

                                                                                                 cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATATAAAATTGGGGCATGTTCTTGAACCCAACGGTAGa                                             >  1:735151/1‑141 (MQ=255)

                                                                                                 aaatgcaaCGATATAACTAGCTCCATTTTTCTTTTATTTTGATGTACGTCTCATAAATACGCCATTTGTAATAAGCTTTGTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGa                                             <  1:321506/134‑1 (MQ=11)

                                                                                                    tGGCATGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCg                                          <  2:476120/141‑1 (MQ=255)

                                                                                                       cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                       <  1:485050/141‑1 (MQ=18)

                                                                                                        aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                      >  1:515322/1‑141 (MQ=255)

                                                                                                             ataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgat                                 <  2:444150/141‑1 (MQ=18)

                                                                                                              taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGACATTAGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGACATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTTAatgata                                >  2:228904/1‑140 (MQ=11)

                                                                                                              taAATAGCTCCATTTGTCTTTTATTTTGATATACGTCTCATCAATACGCCATGTGTAATAAGCTTTTTTATGCTGTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                                <  1:427227/141‑1 (MQ=11)

                                                                                                               aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                               <  1:143897/141‑1 (MQ=255)

                                                                                                                   agttcctttttccttttATTTTGATGTCCGACTCATCAATACTCCATTTGTTATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGt                           <  1:185327/138‑1 (MQ=14)

                                                                                                                       ccATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAGGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCCTATTCTTGAACCCAACGGGAGATCGTTGAATGATACACGttacc                       >  2:59340/1‑138 (MQ=11)

                                                                                                                        cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTaca                      <  1:119578/141‑1 (MQ=40)

                                                                                                                         ctgttCCTTTTATTTTGATATACGTCTCATCAATACGACATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTacac                     <  2:502036/138‑1 (MQ=11)

                                                                                                                         aTTTTCCTTTTATTTTGATGTACGTCTCATCAAGGCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGACCCCAACGGTAGACCGTTGAAGGATGAACGTCTacgc                     <  2:173378/141‑3 (MQ=11)

                                                                                                                          ttttCCTTTTATTTTGATATACGTCTCATCAATACGCTATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                    <  1:198401/141‑1 (MQ=255)

                                                                                                                           tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacca                   <  1:568851/141‑1 (MQ=255)

                                                                                                                            ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATCTTCTTTAACCCAACGGTAGACCGTTGAATGATGAACGTTTAaaccac                  >  2:108250/1‑141 (MQ=255)

                                                                                                                            ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                  >  2:614025/1‑141 (MQ=40)

                                                                                                                              ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                <  1:168598/141‑1 (MQ=18)

                                                                                                                              ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCACCTTTGAGAGACAATTGGATCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                <  1:401318/141‑1 (MQ=11)

                                                                                                                              ccTTTTATTCTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                >  2:539484/1‑141 (MQ=255)

                                                                                                                               cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCGTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtc               >  1:576039/1‑140 (MQ=9)

                                                                                                                               cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAAGAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt               <  1:102679/141‑1 (MQ=255)

                                                                                                                                ttttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTACAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGACTGCTCAACGGTTACACCACGtct              >  2:243342/1‑139 (MQ=11)

                                                                                                                                    tttttTATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt          <  2:511601/140‑1 (MQ=19)

                                                                                                                                    tttttGATATACGTCCCATCAATCCGCCATTTGGAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAATGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt          <  2:149075/140‑1 (MQ=11)

                                                                                                                                       ttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAat       <  2:566070/141‑1 (MQ=18)

                                                                                                                                        tGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAata      <  1:621161/141‑1 (MQ=11)

TTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTC  >  CP000730/36175‑36455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`