BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,378	C→T	87.8%	L58L (TTG→TTA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,378	0	C	T	87.8%	1259.5 / 168.7	696	L58L (TTG→TTA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (41/45); new base T (301/300); total (349/347)
Fisher's exact test for biased strand distribution p-value = 7.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.57e-01

Only 100 of 697 total aligned reads displayed.

CGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACAT  >  CP000730/36238‑36517

cGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTACATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATAGTTTTTTTTCCAATTTTGCTAc                                                                                                                                             >  2:27951/1‑141 (MQ=14)

   aagcatatctctgctgtatgtCCCTCGGTAGCTATGGTACGATATAAATATCTCCATGTGCGTTTTATTTTGATATACGTCTTATACATACGCCATTTGTAATAAGCTTTTTTATGCTTGTTCGTCGAAATTTGATATaaa                                                                                                                                          <  2:683479/127‑1 (MQ=255)

     ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                                                                                                                                        >  2:651204/1‑141 (MQ=38)

      cAAATATCTAATCTATTTCCCTCTGTATCAATGGCACTATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAtt                                                                                                                                       <  2:128081/141‑1 (MQ=11)

        aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATTTAAAATTgg                                                                                                                                     >  2:340847/1‑141 (MQ=255)

        aaTATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAAGCCATTTGTAATAAGCTTTTTTAAGCTTATTCTTCAAAATTTGATATAAAATCgg                                                                                                                                     >  1:60341/1‑141 (MQ=11)

         atatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTGTTATGCTTTTTCTTCCAAATTTGATATAAAATTggg                                                                                                                                    >  2:752049/1‑141 (MQ=255)

          tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTAATTTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                                                                                   <  1:819935/141‑1 (MQ=11)

          tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                                                                                   <  2:743018/141‑1 (MQ=38)

          tatCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                                                                                                                                   <  2:503410/141‑1 (MQ=25)

             cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                                <  1:142288/141‑1 (MQ=255)

             cTAATGTATGTCCCTCTGTATCAAGGGCACGATATAAATAGCTCCATTTTCCTCTTGTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCCTTTTTAGGCTTTTTCTTCCAAGTTGGAAGTAAAATAggggaaa                                                                                                                                >  1:611959/1‑138 (MQ=11)

             agtatttttgtCCCTCTGCAGCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTGCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCat                                                                                                                                <  2:623037/133‑1 (MQ=255)

              tAATCTATGCCCCTCTGCTTCAATGGCACGATATAAATTGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCata                                                                                                                               <  1:195362/141‑1 (MQ=11)

                atgtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                                                                                                                             >  2:418121/1‑141 (MQ=255)

                atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                                                                                                                             >  1:566070/1‑141 (MQ=38)

                 tgtttgtCCCTCTGTATCAATGGTACGATATAAATAGCTCCGTTTTCCTTTTATTTTGATTTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTc                                                                                                                            <  1:799794/141‑1 (MQ=11)

                  gtatgtCCCTCTGCATCAATGGGACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAACACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCt                                                                                                                           <  2:607012/141‑1 (MQ=255)

                    atgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                                                                                         >  2:389491/1‑141 (MQ=255)

                     tgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCTTTTCGCCATTTTTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATTTTAAATTGGGGCATttttttta                                                                                                                        >  1:41909/1‑139 (MQ=11)

                       tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTTATACAAAATTGGGGCATATTCTTTAAc                                                                                                                      >  1:484022/1‑141 (MQ=255)

                         ccTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAccc                                                                                                                    >  2:596029/1‑141 (MQ=255)

                           tctGTATCAATGGCACGATATAAATAGCTCCAGTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCCTATCCTTGAACCCac                                                                                                                  >  2:447051/1‑140 (MQ=11)

                              ttaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                               <  2:771136/139‑1 (MQ=16)

                               taTCAATGTCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTGTTGAACCCAACGGt                                                                                                              >  1:329239/2‑141 (MQ=255)

                                aTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATGTTCTTGGAACAAACGGTa                                                                                                             >  1:455592/1‑141 (MQ=11)

                                 tCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTaa                                                                                                            >  1:301816/1‑140 (MQ=21)

                                  cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATGCTTGAACCCAACGGTAGa                                                                                                           >  1:33660/1‑141 (MQ=255)

                                       gCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTAGGGCATTATCTTGAACCCAACTGTAGATCGtt                                                                                                      >  2:569222/1‑141 (MQ=11)

                                        cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTg                                                                                                     >  1:247341/1‑141 (MQ=21)

                                        cACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTg                                                                                                     <  2:832962/141‑1 (MQ=255)

                                         aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                    >  2:327384/1‑141 (MQ=255)

                                          cGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGaa                                                                                                   >  1:361299/1‑141 (MQ=21)

                                          cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                   >  1:635915/1‑141 (MQ=255)

                                            atatAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatg                                                                                                 >  1:442796/1‑141 (MQ=255)

                                              ataAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTAGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgat                                                                                               >  1:87465/1‑141 (MQ=21)

                                                aaaTATCTCCATTTTCCTTTCATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATCCTCTTTCTTCCACATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAAtgatga                                                                                             <  2:176/141‑1 (MQ=11)

                                                aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATACTTTTTCTTCCAAATTTGATATAAAATTGGGGGATATTATTGAACCCAACGGTAGTTCGTTGTatgatta                                                                                             >  2:462788/1‑139 (MQ=11)

                                                  aTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAAc                                                                                           <  1:764026/141‑1 (MQ=40)

                                                   tAGCTCCATTTTCCTTTTATTTTGATGTACGTGTCATCAATACGCCATTTGTAATAAGGTTTTTTATGCTTTTTCTTTCAAATTTGATATAAAATTGGTGCATATTCTTGAACCCACCGGTAGACCGTTGAATGATGAACg                                                                                          >  2:48185/1‑141 (MQ=11)

                                                    aGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGt                                                                                         >  2:291076/1‑141 (MQ=255)

                                                       tCCATTTTTCTTTTTTTTTGACATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGCTAGTTGAATGATGAACGTTTa                                                                                      <  2:348410/141‑1 (MQ=11)

                                                           ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                  >  2:637837/1‑141 (MQ=25)

                                                           ttttCCTTTCTTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                  <  1:260001/141‑1 (MQ=11)

                                                             ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                                                                                >  1:42754/1‑141 (MQ=21)

                                                             ttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcaccac                                                                                <  2:586327/141‑1 (MQ=40)

                                                             ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATGTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                                <  2:513502/141‑1 (MQ=255)

                                                              tCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGATGAATGATGAACGTTTACACCACg                                                                               <  1:532985/141‑1 (MQ=21)

                                                               ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGt                                                                              <  1:168598/141‑1 (MQ=18)

                                                                cTTTTGTTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAACTTGATATACAATTGAGTCATATGCTTGAACCCAACGGTAGATAGTTGAATGATGAACGTTTACACCACGtt                                                                             <  1:158742/141‑1 (MQ=11)

                                                                cTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCGTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGtc                                                                             >  1:576039/1‑140 (MQ=9)

                                                                cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTGCCAAATTTGACATAAAATTGGGGCTTATTCTTGAACCCAACGGTGGATCGTTGAATGATGAACGTGTACACCACGtt                                                                             >  1:703008/1‑141 (MQ=11)

                                                                cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAAGAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                             <  1:102679/141‑1 (MQ=255)

                                                                cTTTTATTTTGATATACGTCCCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTATTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGCTAGATCGATGAATGATGATCGTTTACACCACGtt                                                                             >  1:508548/1‑141 (MQ=11)

                                                                  tttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTcc                                                                           >  1:677631/1‑141 (MQ=21)

                                                                   ttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAGTCTTGTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGTATGATGAACGTTTACACCACGTTctc                                                                          >  2:549957/1‑139 (MQ=11)

                                                                    tATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTTTTTAACCCAACGGGAGATCGTTGAATGATGAAAGTTTacacaccggtctct                                                                         >  2:664437/1‑131 (MQ=11)

                                                                    tATGTTGATATACGTCTCATCCATACGCCATTTGTAATAAGCTTTTTTATGCTTCTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct                                                                         <  1:737699/141‑4 (MQ=11)

                                                                     tttttGATGTACGTCTAATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGACATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCtt                                                                        <  1:18061/140‑1 (MQ=255)

                                                                     tttttGATATACGTCCCATCAATCCGCCATTTGGAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAATGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCtt                                                                        <  2:149075/140‑1 (MQ=11)

                                                                      ttttGATTGACGTCGCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACCAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTa                                                                       <  1:207852/141‑1 (MQ=255)

                                                                       tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTGCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGATCCCTTCGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                      <  1:827755/141‑1 (MQ=11)

                                                                       tttCATTTACGTCTCATCAATAAGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACGCCACGTTCTCTTaa                                                                      <  1:548021/141‑1 (MQ=11)

                                                                       ttagATGTACGTCTGATCAGTACGCCTTTTGTAATAAGCTTTTTTATGCTTTTCCCTTCAACTTTGATGTAGATGGGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTaa                                                                      <  2:90468/138‑1 (MQ=11)

                                                                          gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                   <  1:714176/141‑1 (MQ=21)

                                                                          gATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCCTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACAACGTTCTCTTAatat                                                                   >  1:78220/1‑141 (MQ=11)

                                                                           atatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGATCTCTTAATAtt                                                                  >  1:691497/4‑141 (MQ=11)

                                                                             gTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTc                                                                <  1:39031/141‑1 (MQ=21)

                                                                              tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGGACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                               >  1:465081/1‑141 (MQ=18)

                                                                              tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGATCCCTTAATATTTCa                                                               >  1:51341/1‑141 (MQ=21)

                                                                               ttttttcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATTTTTTTTAACCCTACGGTAGACCGTTGAATTATGAACGTTTACACCACGTTCCCTTACTATTTAAg                                                              >  2:535818/6‑141 (MQ=11)

                                                                               aCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATACTTTTTCATCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTCCACCCAGTTCCCTTAATATTTCaa                                                              >  2:524403/1‑140 (MQ=11)

                                                                                cGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGa                                                             >  2:696396/1‑141 (MQ=21)

                                                                                    tcatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTCGATGGCTGCATGATGAACGTTTACCACACCTTATCTTACTATTACAgcacat                                                         >  2:730884/1‑136 (MQ=11)

                                                                                      atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCCAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATAGTTGAATTATGAACGTTTACACCACGGTCTCTTAATATTTTAGatatata                                                       >  2:105160/1‑140 (MQ=11)

                                                                                       tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAGACTACGTTCTCTTAATATTTCAGATATATCa                                                      >  1:375561/1‑141 (MQ=11)

                                                                                       tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                      >  1:666001/1‑141 (MQ=255)

                                                                                       tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATAAATCa                                                      >  2:203214/1‑141 (MQ=255)

                                                                                       tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                      >  2:136086/1‑141 (MQ=40)

                                                                                        caATCCCCCATTTGTAATAAGCTTTTGTATGCGTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                     >  2:489743/1‑141 (MQ=11)

                                                                                        caATACGCCATTTGTAATAAGCTTTTTTATGGTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCAc                                                     >  2:366153/1‑141 (MQ=255)

                                                                                        caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                     >  1:756834/1‑141 (MQ=21)

                                                                                          aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGGTATATTACGa                                                   >  1:724868/1‑141 (MQ=255)

                                                                                          aTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                                   >  1:22035/1‑141 (MQ=25)

                                                                                           tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTTTAGGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                  <  1:118879/141‑1 (MQ=11)

                                                                                            aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                 <  2:111431/141‑1 (MQ=255)

                                                                                            aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATa                                                 <  2:308163/141‑1 (MQ=18)

                                                                                             ctccAATTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                <  2:161173/139‑1 (MQ=255)

                                                                                             cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAACTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATag                                                <  1:647992/141‑2 (MQ=255)

                                                                                               ccATTTGTAATAAGCTTTTTTATGCTTTTTTTTTTTTTTTTGATATAAAATTGGGGCATATTCTTTAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGTTCCCTTAATATTTCAGATATATCCCGATAACt                                              >  2:782907/1‑141 (MQ=11)

                                                                                               ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCt                                              >  2:227140/1‑141 (MQ=255)

                                                                                                 aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATTTTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTACTATTTCAGATATATCACGCTAACTCa                                            >  2:540692/1‑141 (MQ=255)

                                                                                                  tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                           <  2:710332/141‑1 (MQ=11)

                                                                                                   ttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAAt                                          <  2:795412/141‑1 (MQ=21)

                                                                                                    tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCACCGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                         >  2:258692/1‑141 (MQ=11)

                                                                                                    tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                         <  1:125845/141‑1 (MQ=18)

                                                                                                     gTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTACTATTTCAGATATATCACGATAGCTCAATgc                                        >  1:316748/1‑140 (MQ=11)

                                                                                                                 tttATGCTTTTTCTTCCAAATTTGATACCAAATTGTTGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAta                            >  1:404323/1‑141 (MQ=255)

                                                                                                                       cTTTTTCTTCCAAATTTGATACAAAATTTGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGcc                      <  1:837981/141‑1 (MQ=25)

                                                                                                                                       tGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATa      <  1:271504/141‑1 (MQ=35)

CGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACAT  >  CP000730/36238‑36517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`