Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,817 A→T 37.1% intergenic (+48/+60) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8170AT37.1% 148.3 / 88.5 100intergenic (+48/+60)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base A (24/38);  new base T (13/24);  total (37/63)
Fisher's exact test for biased strand distribution p-value = 8.31e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

GCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT  >  CP000730/35677‑35955
                                                                                                                                            |                                                                                                                                          
gCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGaa                                                                                                                                            <  1:486845/141‑1 (MQ=255)
gCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGaa                                                                                                                                            <  1:379591/141‑1 (MQ=255)
gCAAATTATACTGAATTTAAAGATGTAGATGCTATACGGGTCAAAGTATTTCGCTGAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATGGTGATCTAATAAAAATAGaa                                                                                                                                            >  1:612987/1‑141 (MQ=255)
   aaTTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGt                                                                                                                                         >  2:166336/1‑141 (MQ=255)
       attCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAATTGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTg                                                                                                                                     <  1:512040/141‑1 (MQ=255)
          cTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTg                                                                                                                                  >  2:679145/1‑141 (MQ=255)
                  aaaGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTAATCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCtaaaaaaaaaaaa                                                                                                                          <  2:649199/141‑13 (MQ=255)
                    aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCCGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaa                                                                                                                        <  2:612987/141‑1 (MQ=255)
                    aGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTTGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTaaaa                                                                                                                        >  1:280919/1‑141 (MQ=255)
                        gaaGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAATTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAat                                                                                                                    <  2:533186/139‑1 (MQ=255)
                        gTAGATGATCTCATTGACAAAGTATTTAATTTAGAAGCTAGTCAGTTGAAAATTTTTGGCGAAAAAGGATGACTTGAACAAGAGCTAAAAGAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAat                                                                                                                    <  1:318336/141‑1 (MQ=255)
                           aatgatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCACAGTTAAAAAAtata                                                                                                                 <  2:568023/140‑1 (MQ=255)
                            atgatCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAg                                                                                                                <  1:475780/141‑1 (MQ=255)
                                 ctcATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAAc                                                                                                           <  1:472113/141‑1 (MQ=255)
                                 ctcATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAAc                                                                                                           <  1:748248/141‑1 (MQ=255)
                                 ctcATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAAc                                                                                                           <  1:34909/141‑1 (MQ=255)
                                 ctcATTGACAAAGTATTTAATGTAGAAGCCATTCAGTATAAATTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAAc                                                                                                           <  1:748347/141‑1 (MQ=255)
                                        aCAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAAt                                                                                                    <  2:632199/141‑1 (MQ=255)
                                          aaaGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAAttt                                                                                                  >  1:247519/1‑141 (MQ=255)
                                            aGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATGAAAATGGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTAt                                                                                                >  2:801891/1‑141 (MQ=255)
                                             gTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACCAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAGAAAATATAGCTAACCACTAATTTATc                                                                                               >  1:514774/1‑141 (MQ=255)
                                                   aaTGTAGAAGCTATTCAGTATAAAGATTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCa                                                                                         >  1:419807/1‑141 (MQ=255)
                                                    aTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAg                                                                                        <  1:519546/141‑1 (MQ=255)
                                                    aTGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAg                                                                                        <  1:832205/141‑1 (MQ=255)
                                                          aaGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCg                                                                                  >  1:718788/1‑141 (MQ=255)
                                                           aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAAAAAAATTGTGATCTCATAAAAATAGAAGGTTCTGAAACAAAGAAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                 <  2:738458/141‑1 (MQ=38)
                                                             cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACGAATTTATCATGTCAGTGTTCGCtt                                                                               <  1:610640/141‑1 (MQ=255)
                                                                  cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                          <  1:358008/141‑1 (MQ=255)
                                                                    gTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                        >  2:147429/1‑141 (MQ=255)
                                                                      ataAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTa                                                                      >  1:220302/1‑141 (MQ=255)
                                                                         aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                   >  1:22430/1‑141 (MQ=255)
                                                                              tttGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCatgatg                                                              <  1:139089/141‑1 (MQ=255)
                                                                                tGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCt                                                            >  1:655806/1‑141 (MQ=255)
                                                                                  gCGAAGAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTaa                                                          <  2:110178/141‑1 (MQ=255)
                                                                                  gCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTaa                                                          >  2:270981/1‑141 (MQ=255)
                                                                                  gCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTaa                                                          >  1:170303/1‑141 (MQ=255)
                                                                                          tGATTACTTGAACAACAGCGGCGCAAAGATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTgg                                                  >  2:181572/1‑141 (MQ=17)
                                                                                            aTTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCa                                                <  1:671244/141‑1 (MQ=255)
                                                                                             ttACTTGAACAAGAGCTAAAAGAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAGGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCag                                               >  1:436035/1‑140 (MQ=255)
                                                                                                cTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAATTAAAAAAATATAGCTAACCACTAATTTATCAGGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                            <  2:72996/141‑1 (MQ=255)
                                                                                                cTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTGATCATGTCAGTGTTCGCTTAACTTGCTAGCGTGATGCTAATTTCGTGGCATGGc                                            <  2:562144/141‑1 (MQ=255)
                                                                                                       aaGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATc                                     <  1:258160/141‑1 (MQ=255)
                                                                                                         gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTTAGTGTTCGCTTAACTTGCTCGCATGATGATAATTTAGTGGCATGGCGAAAATCCg                                   >  1:507400/1‑141 (MQ=255)
                                                                                                         aagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                   <  2:710303/140‑1 (MQ=255)
                                                                                                              aaaataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGAt                              >  1:821036/1‑141 (MQ=255)
                                                                                                                aataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCt                            >  1:632977/1‑141 (MQ=255)
                                                                                                                 acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAGATCCGTAGATCTGaa                         >  2:345561/15‑141 (MQ=11)
                                                                                                                  caaaagagtattCTACTAAAAATGATGGTTCTGTTGCGAAGTAAAAAATATAGCTAATCACTCATATAGCAGGTCAGTGTTCGTTTAACTTGCGAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                        <  2:517678/130‑1 (MQ=11)
                                                                                                                   aaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAAGTTGCTACAATCATGCTAATTTCGTGGCATGCCGAAAATCCGTAGATCTGaa                         >  2:11244/1‑141 (MQ=17)
                                                                                                                   aaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAaga                       <  1:92811/129‑1 (MQ=11)
                                                                                                                    aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATACTAATTTCTTGGCAGCGTCAAAATCCGTAGATCTGAagag                      <  1:616312/130‑1 (MQ=2)
                                                                                                                     aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                     <  1:833569/131‑1 (MQ=11)
                                                                                                                     aagagtattttaataaAAATGATGGTTCTGTCGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                     <  1:445313/131‑1 (MQ=11)
                                                                                                                      agggtattgtaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGGGTTCGTTTAACTTGCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                    <  2:193729/132‑1 (MQ=11)
                                                                                                                      agcgtattttaataaAAATGATGGTTCTGTTGCAAAGAAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                    <  2:706920/132‑1 (MQ=11)
                                                                                                                      agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                    <  1:778908/132‑1 (MQ=11)
                                                                                                                       gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                   >  1:665357/9‑141 (MQ=11)
                                                                                                                       gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                   <  2:537940/133‑1 (MQ=11)
                                                                                                                        tgtgCTCTGATAAAAATAGAAGGTTCTGTTACAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                    <  2:839706/141‑1 (MQ=255)
                                                                                                                        tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                    <  2:222094/141‑1 (MQ=255)
                                                                                                                        tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACGTTCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                    <  2:760745/141‑1 (MQ=255)
                                                                                                                        tgtgATATAGTAAAAATAGCCGGTTATGAGGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                    <  1:393895/141‑1 (MQ=11)
                                                                                                                        agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                  <  1:413813/134‑1 (MQ=11)
                                                                                                                        agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                  <  1:413635/134‑1 (MQ=11)
                                                                                                                        agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                  <  1:375906/134‑1 (MQ=11)
                                                                                                                        agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATCCCGAAAATCCGTAGATCTGAAGAGACCt                  <  2:464790/134‑1 (MQ=11)
                                                                                                                         gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                 >  1:496512/7‑141 (MQ=11)
                                                                                                                         gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                 <  1:698599/135‑1 (MQ=11)
                                                                                                                         gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                 <  1:733475/135‑1 (MQ=11)
                                                                                                                          tgATATAATAAAAATAGAAGGTTCTGTGGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                  <  2:463726/141‑1 (MQ=255)
                                                                                                                          tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATTGCGAAAATCCGTAGATCTGAAGAGACCTgg                >  2:668475/6‑140 (MQ=11)
                                                                                                                          tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                <  1:797411/136‑1 (MQ=11)
                                                                                                                          tattgtaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCAAATCACTAATATATCATGTCAGTGTTCGTTTAACTTACTAGCATGATGCTAATTTCGTGGCATAGCGAAACTCCGTAAAACTGCATAGCCCTGc                >  2:437670/6‑141 (MQ=2)
                                                                                                                           taacTAATAAAAATAGAAGGTTCTATTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                 <  2:149111/138‑1 (MQ=255)
                                                                                                                           attttaataaAAATGATTGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg               <  2:483250/137‑1 (MQ=11)
                                                                                                                           attttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg               <  2:206346/137‑1 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCGCTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg              >  2:278790/4‑141 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGATCTGAAGAGACCTGCgg              >  1:341215/4‑141 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg              >  1:492142/4‑141 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTAAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg              <  2:134401/138‑1 (MQ=11)
                                                                                                                            ttttaataaAAATGATGGTTCTGCTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg              <  1:152758/138‑1 (MQ=11)
                                                                                                                            aTCTATTAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                <  2:609559/141‑1 (MQ=255)
                                                                                                                            aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                <  1:102870/141‑1 (MQ=255)
                                                                                                                            aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                <  1:206777/141‑1 (MQ=255)
                                                                                                                             tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAACCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCTACAATCCGTAGATCTGAAGAGGCGTGCggg             >  2:534774/3‑140 (MQ=11)
                                                                                                                              ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCGGTAGAACTGAAGAGACCGGCGGtt            >  2:496364/2‑141 (MQ=11)
                                                                                                                              ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt            <  2:49125/140‑1 (MQ=11)
                                                                                                                               taataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt             <  2:655806/141‑1 (MQ=40)
                                                                                                                               taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc           <  1:427491/141‑1 (MQ=11)
                                                                                                                               taataaAAATGATGGTTCAGTTGCAAAGTAAACAATATCGCTACTCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTATCATGATGCTAATTTCGTGGCATGTCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc           <  2:827263/141‑1 (MQ=11)
                                                                                                                                aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt          >  2:127452/1‑141 (MQ=11)
                                                                                                                                aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTATTCGTTTAACTTGCTAGCATGATGCTAATTTCGTCGCATGCCGAAAATCCGTAGATCTGAAGAGCCCTCGGGTTCt          >  2:174776/1‑141 (MQ=11)
                                                                                                                                 ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt         >  2:21499/1‑141 (MQ=11)
                                                                                                                                  taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt        <  1:84329/141‑1 (MQ=11)
                                                                                                                                  aaaaaaaTGAAGGTTCTGATGCAAAGTAAAAAATATAGCTAATCACTAATATAACATATAAGAGAACGTTTAACTTGCTAACATGTTGCTAATACCGAGGACTTGCGAAAATCCGTAGATCTGAAGAGAACTGCGGTTCtt         >  2:250293/2‑141 (MQ=2)
                                                                                                                                     aaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGTTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtttt       <  1:166336/141‑1 (MQ=25)
                                                                                                                                        tAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCCGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat    <  1:679145/141‑1 (MQ=17)
                                                                                                                                        tAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat    <  2:263973/141‑1 (MQ=32)
                                                                                                                                         tgAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACGAATTTGTCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata   <  2:643114/140‑1 (MQ=14)
                                                                                                                                          gAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCAAGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtctat  >  1:478299/1‑141 (MQ=14)
                                                                                                                                            |                                                                                                                                          
GCAAATTATTCTGAATTTAAAGATGTAGATGATCTCATTGACAAAGTATTTAATGTAGAAGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT  >  CP000730/35677‑35955

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: