Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 36,581 C→T 27.1% intergenic (‑30/‑493) USA300HOU_0026 ← / → USA300HOU_0028 IS431mec transposase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073036,5810CT27.1% 198.8 / 78.4 126intergenic (‑30/‑493)USA300HOU_0026/USA300HOU_0028IS431mec transposase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (40/51);  new base T (17/17);  total (57/69)
Fisher's exact test for biased strand distribution p-value = 5.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 126 total aligned reads displayed.

CCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTTA  >  CP000730/36444‑36719
                                                                                                                                         |                                                                                                                                           
ccTTAATAGTGCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACtat                                                                                                                                          <  1:266046/141‑1 (MQ=17)
  ttAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaa                                                                                                                                        >  1:30189/1‑141 (MQ=33)
   tAATATTTTATATAAATCACGATAGCTCAATGAATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATaaa                                                                                                                                       <  1:412/141‑1 (MQ=11)
   tAATATTTCAGATATAGCACGATAACTCAATGTATATCTTAGATAATAGCCAACTGCTACAGTTATAATATCCTTGTTAAATTGTTTATATCTCAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATaaa                                                                                                                                       <  2:247909/141‑1 (MQ=11)
       atgtCAGCTATATCACGACAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCa                                                                                                                                   <  1:108250/138‑1 (MQ=255)
       atTTCCGATATATCACGATAACTCCATGTACATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCa                                                                                                                                   <  1:358286/141‑1 (MQ=255)
       atTTCAGATATATCACGATCACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCa                                                                                                                                   <  1:17475/141‑1 (MQ=25)
          ttaGATATATCACGATCACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACt                                                                                                                                <  1:27951/139‑1 (MQ=25)
            aGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACttt                                                                                                                              >  1:102941/1‑141 (MQ=35)
               tatatCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCa                                                                                                                           <  1:83144/141‑1 (MQ=35)
                     aCGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGaa                                                                                                                     >  1:96305/1‑141 (MQ=35)
                         tagcTCAATGCATATCTTAGATAGTAGCCATCGGCTACAGTGATATCGACCTTGTTAAATTGTTTATATCTCAAATAGTTTATACATAATACTCCTTTTTTTTAAAATTATATTATTAATTCAACTTTGCAACAGAACCGt                                                                                                                 <  2:31402/138‑1 (MQ=11)
                         tagcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGt                                                                                                                 >  2:252535/4‑141 (MQ=2)
                         tAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGt                                                                                                                 <  1:143588/141‑1 (MQ=37)
                           gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTACTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                               >  1:662117/2‑141 (MQ=12)
                           gcTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACAGCCTGGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtat                                                                                                               >  1:795425/2‑141 (MQ=255)
                            cTCAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatt                                                                                                              >  1:86602/1‑141 (MQ=12)
                             tCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGtatta                                                                                                             >  1:332464/1‑141 (MQ=40)
                             tCAATGCATATCTTAGATAGTAGCCAACCGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt                                                                                                             <  1:10620/141‑2 (MQ=255)
                             tCAATGCATATCTTAGATAATAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattt                                                                                                             >  1:469163/1‑140 (MQ=255)
                              cAATGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTAATTTAAAGTATATTATAAATTCACCTTTGCAACAGAACCGtatttt                                                                                                            >  1:567633/1‑139 (MQ=11)
                               aaTGCATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtatttta                                                                                                           >  1:762678/1‑138 (MQ=255)
                               aaTGAATATCTTAGATAGTAGCCAACTGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaact                                                                                                           <  2:345749/141‑9 (MQ=11)
                                atgcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttat                                                                                                          >  1:22127/5‑137 (MQ=255)
                                 tgcatatCTTAGATAGTAAGCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatg                                                                                                         <  1:85758/138‑6 (MQ=11)
                                 tgaatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGCCTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgt                                                                                                         <  2:664464/138‑11 (MQ=11)
                                  gcatatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatga                                                                                                        <  1:276641/139‑7 (MQ=255)
                                   catatatTAGATAGTAGCCAACAGCTACAGTGATAACAAAATTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTACTTTTTGTTAAAATTATACTATGAATTCAACTTTACAACAGAAcctcttttactata                                                                                                       >  2:17777/2‑129 (MQ=11)
                                   catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtat                                                                                                       <  1:307722/140‑13 (MQ=11)
                                   catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtat                                                                                                       >  1:255493/2‑129 (MQ=11)
                                   catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtat                                                                                                       <  1:648907/140‑13 (MQ=11)
                                   catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgag                                                                                                       >  1:50106/2‑134 (MQ=255)
                                   catatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgag                                                                                                       >  1:629694/2‑134 (MQ=255)
                                    gtatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                                                                                                      <  2:103718/140‑14 (MQ=11)
                                    atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATCGTTCATACAGAAGACTCCTTTTTGTTAAAATGATACTATAACTTCAACTTTGCAACAGAACCGTATTATGGAATa                                                                                                      >  1:190792/1‑141 (MQ=255)
                                    atatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTTTTTTTTTTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATa                                                                                                      >  2:676832/1‑141 (MQ=14)
                                    atatCTTAGATAGTAGCCAACAGTTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGTAGACTCCTTTTTGTTAAAATTAGACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                                                                                                      <  2:238374/141‑14 (MQ=11)
                                    atatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                                                                                                      <  1:791634/141‑14 (MQ=11)
                                    atatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctcatttactatat                                                                                                      >  1:409768/1‑128 (MQ=11)
                                     tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGATcctcctaactgtatta                                                                                                     <  1:641070/141‑15 (MQ=11)
                                     tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtattg                                                                                                     >  2:796596/1‑127 (MQ=11)
                                     tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAAcctcctaactgtatta                                                                                                     >  2:667416/1‑127 (MQ=11)
                                     tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagta                                                                                                     <  2:768772/141‑10 (MQ=255)
                                     tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagta                                                                                                     <  1:773756/141‑10 (MQ=255)
                                     tatCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATGGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGGAcctcctaactgtatta                                                                                                     >  2:450868/1‑127 (MQ=11)
                                     tatCTTAGATAGTAGCCAACAGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAAcctaatttactatctg                                                                                                     >  2:805930/1‑127 (MQ=11)
                                         ttAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGAt                                                                                                 <  1:765449/141‑1 (MQ=255)
                                          tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATATTGCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATg                                                                                                <  2:803464/141‑1 (MQ=255)
                                          tAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATATTATAAATTCAACTTTGCAACAGAACCGtattttatgagtacactc                                                                                                <  2:265660/141‑15 (MQ=11)
                                           aGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGt                                                                                               <  1:384304/141‑1 (MQ=255)
                                           aGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGt                                                                                               <  2:49061/141‑1 (MQ=255)
                                              tagtagGCAACGGCTACAGTGATAACTTCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTgg                                                                                            <  2:188222/141‑1 (MQ=255)
                                              tagtagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTgg                                                                                            <  2:404323/141‑1 (MQ=255)
                                               agtagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGt                                                                                           >  2:555297/1‑141 (MQ=255)
                                                 tagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTaa                                                                                         <  2:470750/141‑1 (MQ=255)
                                                 tagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTaa                                                                                         <  2:470960/141‑1 (MQ=255)
                                                 tagCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTaa                                                                                         >  1:309791/1‑141 (MQ=255)
                                                     cAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAtt                                                                                     <  1:185256/141‑1 (MQ=255)
                                                     cAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACAtt                                                                                     <  1:369046/141‑1 (MQ=255)
                                                       aCGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTta                                                                                   <  1:667835/141‑1 (MQ=255)
                                                         ggCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTtata                                                                                 >  2:152623/1‑141 (MQ=255)
                                                         ggCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTaata                                                                                 >  2:365174/1‑141 (MQ=255)
                                                          gCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGAATTATGGAATAGAGATGTTGGTAACATTtataa                                                                                >  2:486642/1‑140 (MQ=255)
                                                            tACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAg                                                                              >  1:643195/1‑141 (MQ=255)
                                                                 ggATAACATACTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCa                                                                         <  2:581478/140‑1 (MQ=255)
                                                                   aTAACATCCTTGTTACATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCAtt                                                                       <  1:522802/141‑1 (MQ=255)
                                                                        aTCCTTGGTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACt                                                                  <  1:245457/141‑1 (MQ=255)
                                                                          ccTTGTTAAATAGTTTATATCTGACACCCATCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTa                                                                >  2:348251/1‑141 (MQ=25)
                                                                            ttGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAg                                                              <  1:171911/141‑1 (MQ=255)
                                                                              gTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGtt                                                            >  1:461186/1‑141 (MQ=255)
                                                                              gTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGtt                                                            >  1:10832/1‑141 (MQ=255)
                                                                               ttAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGttt                                                           >  1:739366/1‑141 (MQ=255)
                                                                                tAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTa                                                          <  2:71894/141‑1 (MQ=255)
                                                                                 aaaTTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTaa                                                         >  1:576782/1‑141 (MQ=255)
                                                                                    ttGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAAttt                                                      <  2:2874/141‑1 (MQ=255)
                                                                                         tataTCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGtta                                                 >  1:323747/1‑141 (MQ=255)
                                                                                             tCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAACCTCATCCATGGACTTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTAc                                             >  1:493538/1‑141 (MQ=37)
                                                                                                taaaTAGTTTAGACATAAGTCTCCTTTTTGTTAAAATTATCCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGTCAACATTCATTCAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGa                                         <  1:789928/140‑1 (MQ=25)
                                                                                                gAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGa                                         >  2:638495/1‑141 (MQ=255)
                                                                                                gAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATAAAGGGTCATTATACTTAAGTTTAGTTTCGTTATTACAGa                                         >  1:294390/1‑141 (MQ=255)
                                                                                                     aGTTCATTAAGATGACTCGTTTTTGTTAGAATTATACNTTAAATTCAACTTTGCAACAGAACCGTATTATGGAGTAGAGATGTTGGTAACATTGATACAGGATCAATATACTTAAGTTTTATTTCGTTATTACAGAACcac                                    <  2:541288/141‑1 (MQ=37)
                                                                                                     aGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcac                                    <  1:152623/141‑1 (MQ=255)
                                                                                                     aGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcac                                    <  1:57402/141‑1 (MQ=255)
                                                                                                     aGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcac                                    <  1:333053/141‑1 (MQ=255)
                                                                                                      attactACAGAAGCCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTTTACAGGCTCATTATACTTAAGTTTAATTTCGTTATTACAGAACcaca                                   <  2:544179/136‑1 (MQ=255)
                                                                                                       ttCAAACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcacac                                  <  1:149682/141‑1 (MQ=255)
                                                                                                        tCATACATAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACcacaca                                 >  1:527086/1‑141 (MQ=255)
                                                                                                        tCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGGTGTTGGTAACATTTATACAGGATCATTATAATTAAGTTTAATTTCGTTATTACAGAACcacaca                                 >  1:217100/1‑141 (MQ=255)
                                                                                                           aaCAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTc                              <  1:90471/140‑1 (MQ=255)
                                                                                                                 agaCTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCa                        >  1:678140/1‑141 (MQ=255)
                                                                                                                    cTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCagaa                     >  1:263990/1‑141 (MQ=255)
                                                                                                                      ccTTTTTTTTTTTATTATACTATAAATTCAACTTTGCAACAGAAACGTATTATGGAATAGAGCTGATGGTAGCATTTATACAGGAACATTATACTTAAGTTTAATTTTGTATTTCATGAACCACACATTCCAACCagaaga                   >  2:458094/1‑141 (MQ=255)
                                                                                                                         ttttGTTAAAATGATAATATAAATTCAATGTTTCAACAGATCCGTGTTATGGAATAGAGATGGTGGTAACATTTATACAGGATCATGATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGaa                <  1:155086/141‑1 (MQ=255)
                                                                                                                            tGTTAAAATTATACTATAAATTCAACTTCGTAACAGATCCCTATTATTGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTACGTCTACTTTCGCTATTACAGAACCACACACTCCAACCAGACGAGAAAGt             <  2:718788/141‑1 (MQ=255)
                                                                                                                              ttAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTAt           >  2:749655/1‑141 (MQ=255)
                                                                                                                                 aaaTTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATCGAGATGTTGGTAACATTTATACAGGATCCTTATACTTAAGTTTAATTTCGTTATTACAGAACCACCAATTCCAACCAGAAGAGAAAGTATGTc        >  2:413482/1‑141 (MQ=255)
                                                                                                                                    ttATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTAt     <  1:373771/141‑1 (MQ=255)
                                                                                                                                    ttATACTATAAATTCAACTTTGCAACAGAACAGCATTATGGAATAGAGATGTTGATGAGAATTATACAGGATCATTATACTTGAGTTTAATTTGGGTATGACAGAACCACACATTCCAACCAGAAGAGAAAGTATggccat     >  2:294825/1‑136 (MQ=255)
                                                                                                                                       taCTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTTa  >  1:720728/1‑141 (MQ=255)
                                                                                                                                       taCTATAAATTCAAATTTGAAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTAtttg  >  1:546438/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                           
CCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCATACAGAAGACTCCTTTTTGTTAAAATTATACTATAAATTCAACTTTGCAACAGAACCGTATTATGGAATAGAGATGTTGGTAACATTTATACAGGATCATTATACTTAAGTTTAATTTCGTTATTACAGAACCACACATTCCAACCAGAAGAGAAAGTATGTCTATTTA  >  CP000730/36444‑36719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: