Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,265 C→T 27.7% intergenic (+323/‑811) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2650CT27.7% 680.4 / inf 555intergenic (+323/‑811)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base C (222/174);  new base T (76/78);  total (303/252)
Fisher's exact test for biased strand distribution p-value = 1.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

Only 100 of 559 total aligned reads displayed.

AAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGA  >  CP000730/76125‑76404
                                                                                                                                            |                                                                                                                                            
 aGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCg                                                                                                                                             >  1:352501/1‑141 (MQ=255)
                  atTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGa                                                                                                                           >  2:796977/1‑141 (MQ=255)
                    tataGCAAAATATAGGTTCTGTTGAAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGata                                                                                                                         <  2:347322/141‑1 (MQ=255)
                          acaaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAATAGGAGTCTTCTGTATGAACTATTTCAGATATAAACGATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCAtt                                                                                                                   >  2:618165/3‑141 (MQ=255)
                           attaggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTg                                                                                                                  <  2:825438/135‑1 (MQ=255)
                           aaaTATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGCTATGCATTg                                                                                                                  >  1:188019/1‑141 (MQ=255)
                             taggaGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGCACTCGAGCGGAGATAAATCATGTAACGGTGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                                                                                                                <  1:826271/137‑1 (MQ=11)
                             aagatggTACTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAg                                                                                                                <  2:708719/136‑1 (MQ=255)
                              atgaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAAAAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGt                                                                                                               <  2:166880/138‑1 (MQ=255)
                                taGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTa                                                                                                             >  1:128595/1‑141 (MQ=37)
                                 aGGTTCTGTTGCAAAGTTGAATTTATAATATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTAt                                                                                                            >  1:762646/1‑141 (MQ=0)
                                 aGGTTCTGTTGCAAAGTCGAATTTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTATCTGTTGGCTACTATCTAAGATATGCATTGAGTTAt                                                                                                            <  1:31220/141‑1 (MQ=2)
                                                gTTGAATCTATAGTATCATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaa                                                                                             >  1:429732/1‑141 (MQ=25)
                                                        tataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAg                                                                                     >  2:736185/1‑141 (MQ=32)
                                                         ataatataatTTAAACAAAAAGGCGTCTTCTGTACGAACTAGTTCAGATATAAACAATTTAACAAGGATGTTATCACGGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAga                                                                                    <  1:161583/141‑2 (MQ=11)
                                                         ataatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAga                                                                                    >  1:90141/5‑140 (MQ=12)
                                                         ataataTAAGTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAga                                                                                    <  1:492541/141‑2 (MQ=255)
                                                         ataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAgg                                                                                    <  1:79666/141‑1 (MQ=17)
                                                         ataGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAgg                                                                                    <  1:625954/141‑1 (MQ=32)
                                                         agaGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAgg                                                                                    <  1:100837/139‑1 (MQ=17)
                                                          taGTATAATTTTAACAAAAAGGAGTCTTCTGGATGAACTATTTCAGGTATTAATATTTGCACAAGGCTTTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAggg                                                                                   <  2:321838/141‑1 (MQ=11)
                                                           aatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgaga                                                                                  <  2:762646/139‑4 (MQ=12)
                                                           aGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCCTTGAGTTATCGTGATATATCTGAAATATTAAGGGa                                                                                  <  1:199717/141‑1 (MQ=17)
                                                              ataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAACCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACg                                                                               <  1:670992/141‑1 (MQ=255)
                                                                aaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtg                                                                             <  2:240042/141‑1 (MQ=17)
                                                                 aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtgg                                                                            <  2:378049/141‑1 (MQ=17)
                                                                  ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtggt                                                                           <  1:59492/141‑1 (MQ=12)
                                                                   tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggtg                                                                          <  1:383976/141‑1 (MQ=17)
                                                                   tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggtg                                                                          >  1:541285/1‑141 (MQ=32)
                                                                    ttAACAAAAAGGAGTCTTCTTTATGAACTACTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                                                         <  2:658530/141‑1 (MQ=12)
                                                                     tAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtttacac                                                                        <  1:717846/141‑7 (MQ=255)
                                                                     tAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGACTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGACATATTAAGAGAACGTGGTGTa                                                                        >  2:393050/1‑141 (MQ=255)
                                                                      aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCCTTGAGTTATCGTGATATATCTGAAATATCAAGGGAACGTGGGGTaa                                                                       >  1:55850/1‑141 (MQ=255)
                                                                      aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTaa                                                                       >  1:639120/1‑141 (MQ=32)
                                                                      aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGATACGATCTACGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                       >  2:504738/1‑141 (MQ=11)
                                                                        cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAc                                                                     <  1:693312/141‑1 (MQ=32)
                                                                        cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAAc                                                                     <  1:577616/141‑1 (MQ=17)
                                                                          aaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGt                                                                   >  1:664437/1‑141 (MQ=17)
                                                                           aaaGGAGTCTTCTGTATGAACTATTTAAGATATAAACAATTTAACAAGGATGTTCTCACTGTCGCCGTTGGCTACTATCACAGATATGCCTTGACCTACCGGGATAGAACGGAAATAGTAAGAGAACGTGGTGTAAACGtt                                                                  >  2:486363/1‑141 (MQ=11)
                                                                             aGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtca                                                                <  1:471581/141‑1 (MQ=17)
                                                                               gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatc                                                              >  2:258046/1‑141 (MQ=17)
                                                                                aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatca                                                             <  2:406393/141‑1 (MQ=17)
                                                                                 gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTcatcat                                                            <  2:42462/141‑1 (MQ=17)
                                                                                 gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACGGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATAGCTGAAATATTAAGGGAACGTGGGGTAAACGTTcatcat                                                            >  1:485957/1‑141 (MQ=12)
                                                                                   cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTc                                                          <  1:294324/141‑1 (MQ=12)
                                                                                   cctctGTCGGAACTATTGCAGATAGAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTc                                                          <  2:574346/139‑1 (MQ=11)
                                                                                      ttGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAAc                                                       <  2:783387/140‑1 (MQ=12)
                                                                                       tGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACg                                                      >  2:628938/1‑141 (MQ=17)
                                                                                           tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCt                                                  >  2:208138/1‑141 (MQ=17)
                                                                                           tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCt                                                  >  1:108088/1‑141 (MQ=12)
                                                                                             aaCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGCAATATTAAGAGAACGTGGTGTAAACGTTGATCATTCAACGATCTAc                                                >  1:243342/1‑141 (MQ=11)
                                                                                             aaCTATTTCAGATATAAACAATTTAACAAGGATGGTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTCAGCTATCGTGATATATCTGACATATTACGAGAACGTGGTGTAAACGTTCATCATTCGACGATATAc                                                >  1:283769/1‑141 (MQ=11)
                                                                                               cTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCACCGGTCTACCg                                              >  2:342879/1‑141 (MQ=17)
                                                                                                gaggtCAGATATAGACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGt                                             <  2:836670/137‑1 (MQ=11)
                                                                                                  tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTGCCCGTAAGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTg                                           <  1:229932/141‑1 (MQ=11)
                                                                                                  tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTg                                           >  1:372802/1‑141 (MQ=17)
                                                                                                  tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTGCATCATTCAACGGTCAACCGTTg                                           >  2:29390/1‑141 (MQ=12)
                                                                                                  tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATCTCTGCAATATGAAGGGAACGTGGTGTACACGTTCATCATTCAACGGTCTACCGTTg                                           <  1:530832/141‑1 (MQ=255)
                                                                                                  tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCGTCATTCAACGGTCTACCGTTg                                           >  1:21876/1‑141 (MQ=17)
                                                                                                  ttgtgtagataAACAAGTGAATTAGGATGTTATCAATGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATAGATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTg                                           <  1:738616/133‑1 (MQ=11)
                                                                                                   ttCTGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTgg                                          <  1:814323/141‑1 (MQ=255)
                                                                                                   ttCAGATATAAACCATTTAACAAGGAGGCTATCACTGGAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTgg                                          <  1:17064/141‑1 (MQ=11)
                                                                                                   ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTgg                                          >  2:119950/1‑141 (MQ=17)
                                                                                                   ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTgg                                          <  2:277330/141‑1 (MQ=32)
                                                                                                   ttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTgg                                          >  2:45557/1‑141 (MQ=12)
                                                                                                    tgaGATACAAACAATTTAACAAGGATATTATCATTGTACCTGTTGGCTACTAGCTAATATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTCGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                         <  2:70184/139‑1 (MQ=11)
                                                                                                    tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTggg                                         <  2:462316/141‑1 (MQ=17)
                                                                                                     cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTCGATCATTCAACGGTCTACCGTTGGGt                                        >  1:630589/1‑141 (MQ=12)
                                                                                                      aGATATAAACACTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTATTATCTAAGATATGCATTTAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                       <  2:144078/141‑1 (MQ=11)
                                                                                                      aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGtt                                       >  2:442405/1‑141 (MQ=17)
                                                                                                         tataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCaa                                    <  1:772438/141‑1 (MQ=17)
                                                                                                         tataAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCaa                                    >  2:726554/1‑141 (MQ=12)
                                                                                                          ataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                   >  1:726077/1‑141 (MQ=17)
                                                                                                            aaaCAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGaa                                 >  1:122849/1‑141 (MQ=17)
                                                                                                             aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTCCCGTTGGGTTCAAGAat                                >  1:339671/1‑141 (MQ=255)
                                                                                                             aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAat                                >  1:837330/1‑141 (MQ=12)
                                                                                                             aaCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATAATTCAACGATCTACCGTTGGGTTCAAGAat                                >  2:530842/1‑141 (MQ=11)
                                                                                                              aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGCata                               >  1:325928/1‑141 (MQ=255)
                                                                                                               cAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGGatat                              >  1:4587/1‑141 (MQ=17)
                                                                                                                aaTTTAACAAGGATGTTATCCATGTGCCGAGGGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATg                             >  1:405274/1‑141 (MQ=11)
                                                                                                                 aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGc                            >  2:477989/1‑141 (MQ=12)
                                                                                                                  tttAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcc                           <  2:132823/141‑1 (MQ=17)
                                                                                                                    tAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGcccc                         <  1:96101/141‑1 (MQ=32)
                                                                                                                    tAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTTCCGTTGGGTTCAAGAATATGcccc                         <  1:611021/141‑1 (MQ=255)
                                                                                                                      aCAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCaa                       >  2:641663/1‑141 (MQ=17)
                                                                                                                       cAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAt                      >  2:264487/1‑141 (MQ=17)
                                                                                                                        aaGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGTCCCAAtt                     >  1:255201/1‑141 (MQ=17)
                                                                                                                        aaGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAAGATGCCCCAAtt                     >  2:581116/1‑141 (MQ=17)
                                                                                                                            aTGTTATCACTGTAGCCGTTGGCTATTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCGCCATTTtat                 >  2:808190/1‑141 (MQ=11)
                                                                                                                            aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATTGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCTTCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                 <  2:546800/141‑1 (MQ=11)
                                                                                                                              gTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTatat               >  2:227182/1‑141 (MQ=255)
                                                                                                                              gTTATCACTGTAGCCGATGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtatgt               >  1:416284/1‑139 (MQ=11)
                                                                                                                                 aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTTAGTTATCACAAGATCTAAAAAATATTAAGGGAACGTTGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaa            <  1:785292/141‑1 (MQ=11)
                                                                                                                                   cACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAt          >  1:141591/1‑141 (MQ=34)
                                                                                                                                    aCTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAtt         >  1:703978/1‑141 (MQ=255)
                                                                                                                                     cTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAAttt        <  2:669050/141‑1 (MQ=25)
                                                                                                                                      tGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg       <  1:481184/141‑1 (MQ=25)
                                                                                                                                       gTAGCTGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTTGTGTAAACGTTCATCATTCAACGATCTACCGTTGCGTTCAATAATATGCCCCAATTTTATATCAAAttttg      <  2:683483/141‑3 (MQ=11)
                                                                                                                                       gTAGCCGTTGGCTACTATCTAAGGTATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg      <  2:188019/141‑1 (MQ=25)
                                                                                                                                       gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCACCGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg      >  1:51493/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                            
AAGGCAATTAATTTAATAATTATAGCAAAATATAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGA  >  CP000730/76125‑76404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: