Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 110,651 C→T 11.2% D170D (GAC→GAT USA300HOU_0109 → staphylococcal tandem lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730110,6510CT11.2% 203.5 / 11.0 83D170D (GAC→GATUSA300HOU_0109staphylococcal tandem lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (27/44);  new base T (7/2);  total (36/47)
Fisher's exact test for biased strand distribution p-value = 3.26e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGT  >  CP000730/110511‑110789
                                                                                                                                            |                                                                                                                                          
aaaaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGAc                                                                                                                                            >  1:547175/1‑141 (MQ=255)
  aaGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTCCAGCAAGGGAGATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACgg                                                                                                                                          <  1:398206/141‑1 (MQ=18)
  aaGATATCCGGGTAAAATGGTAGATAATAAAAACATTCCAACAAAAGAAATTAAAAATACCGAGTTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACgg                                                                                                                                          <  1:822660/141‑1 (MQ=17)
    gATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTGCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGgag                                                                                                                                        <  2:47795/141‑1 (MQ=255)
       atCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGAAGata                                                                                                                                     >  1:583486/1‑141 (MQ=255)
           ggTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTc                                                                                                                                 <  1:76984/141‑1 (MQ=255)
            gTTAAAATGGTCGATAATAGAATCATTCCAACAAAAGAGATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTAGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCa                                                                                                                                >  1:467947/1‑141 (MQ=255)
              tAAAAGTGTCGATAATATAATCACTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCata                                                                                                                              <  2:39737/141‑1 (MQ=255)
                 aaTGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATaa                                                                                                                           >  1:768554/1‑141 (MQ=255)
                    ggTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATatcatca                                                                                                                        >  1:816124/1‑140 (MQ=255)
                    ggTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATcc                                                                                                                        <  1:597238/141‑1 (MQ=255)
                      tCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTCCCTCGCTTCTACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTAATATAATCCag                                                                                                                      >  1:397692/1‑141 (MQ=17)
                      tCGATAATAAAATCATTCCAAAAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCag                                                                                                                      <  1:316347/141‑1 (MQ=255)
                        gATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCagag                                                                                                                    <  1:382143/141‑1 (MQ=255)
                            ataaAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGc                                                                                                                <  1:470421/141‑1 (MQ=255)
                               aaaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATTTAGAGGTGCCGa                                                                                                             >  1:84475/1‑141 (MQ=255)
                                aaTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAg                                                                                                            <  2:654380/141‑1 (MQ=255)
                                 aTCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGt                                                                                                           <  1:408459/141‑1 (MQ=255)
                                 aTCAATCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGt                                                                                                           <  2:674777/141‑1 (MQ=255)
                                     ttCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTAtt                                                                                                       <  1:499066/141‑1 (MQ=255)
                                        acaagaaaGAAATTAAAATGGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTGGTTCAATATGGCGACTTTAAAAATTAGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCgg                                                                                                    <  2:816102/136‑1 (MQ=17)
                                            aaaaGAAACTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAAGTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTaa                                                                                                <  1:741140/141‑1 (MQ=255)
                                              aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                              <  1:748170/141‑1 (MQ=255)
                                              aaGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAat                                                                                              <  1:307421/141‑1 (MQ=255)
                                               aGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATATTGCGATGGGGCCGAGTTATTCGGCTAAata                                                                                             <  1:616012/141‑1 (MQ=18)
                                                gAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAatat                                                                                            >  2:43979/1‑141 (MQ=255)
                                                gAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATACAGAGATGCCGAGTTATTCGACTAAatac                                                                                            >  2:64077/1‑140 (MQ=255)
                                                   aTTAAAGATGGAAAAATAAAAAAAGAAATCGAAAACTTTAAATTCTTAGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTAATCGGCTAAATCTCaa                                                                                         >  2:364344/1‑141 (MQ=17)
                                                       aataTGAAAAAATAAAAAAAGAAATCAAAAACTTTAAGTTCTTTGTTCAATATGGCAACTTTAAAAATTTGAAAAATTATAAAGAAGGAGATTTTTCATATAATCCAAAAATGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                     >  2:174075/4‑141 (MQ=17)
                                                       aagaagAAAAAATAAAAAAAGAAATCGCAAACTTTAAGTTCTTTGTTCAATATGGCGATTATAAAAAAAAAAAAAATTATAAAGACTGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTaa                                                                                     <  2:198025/141‑1 (MQ=17)
                                                               aaaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgat                                                                             <  2:720764/141‑1 (MQ=255)
                                                                aaataaaaaaataaaaCGAAAACTTTAAGTTTGTTGTTCAATATGGCGAATTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                            <  1:170114/141‑1 (MQ=17)
                                                                aaaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatg                                                                            >  1:634572/1‑141 (MQ=255)
                                                                 agaaaaaaaaGGAATCGCAACCTTTAAGGTCTTTGTTCAATATGGTGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                           <  1:393473/138‑1 (MQ=14)
                                                                 aaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAatgatgg                                                                           >  2:324804/1‑140 (MQ=255)
                                                                 aaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                           <  1:364736/141‑1 (MQ=255)
                                                                 aaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                           >  2:716121/1‑141 (MQ=255)
                                                                 aaTAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAAtgatga                                                                           <  1:742522/141‑1 (MQ=255)
                                                                     aaaaaaGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtat                                                                       >  1:117103/1‑141 (MQ=255)
                                                                      aaaaaGAGATCGATGACTTGATGTTCGTTGGTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATtata                                                                      >  2:490052/1‑141 (MQ=17)
                                                                             aaTCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTaaa                                                               >  2:262543/1‑141 (MQ=255)
                                                                               tCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTACTGATGATTATAATGTAAAAc                                                             <  1:525399/141‑1 (MQ=255)
                                                                                 gAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTCAATGTCAATTAACTAATGATGATTATAATGTAcaacaa                                                           >  1:69074/1‑141 (MQ=255)
                                                                                 gAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACaa                                                           <  2:547175/141‑1 (MQ=255)
                                                                                 gAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACaa                                                           <  2:172205/141‑1 (MQ=255)
                                                                                  aaaaCTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                          <  1:60921/141‑1 (MQ=255)
                                                                                  aaaaCTTTAAGTTATATGTTCAATATGGCGACTATAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAAt                                                          <  1:699937/141‑1 (MQ=255)
                                                                                    aaCTTTAAGTTCTTTGTTCAGTATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGAAGATATTTCATATAATGCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTCATGATGATTATAATGTTAAACAATTa                                                        >  1:511558/1‑141 (MQ=17)
                                                                                    aaCTTTAAGTTCTGTGGTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTa                                                        <  2:131368/141‑1 (MQ=255)
                                                                                      cTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACg                                                      <  1:786872/141‑1 (MQ=255)
                                                                                       tttAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGc                                                     <  2:515752/141‑1 (MQ=255)
                                                                                         tAAGTTCTTTGTTCAATATGGCGTCTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCTGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                   >  2:599378/1‑141 (MQ=255)
                                                                                         tAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaa                                                   <  1:716121/141‑1 (MQ=255)
                                                                                           aGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCaaaa                                                 >  2:241598/1‑141 (MQ=255)
                                                                                               ttttGTTCGATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATGTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAgata                                             <  2:133115/140‑1 (MQ=255)
                                                                                                      aaaTATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGAtata                                      <  1:26644/140‑1 (MQ=255)
                                                                                                             gCGACTTTAAAAATTTGAAAAATTATAGAAACTGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACcgacga                               >  1:200340/1‑141 (MQ=255)
                                                                                                                aCTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTCATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTa                            >  1:600442/1‑141 (MQ=255)
                                                                                                                  tttAAAGATTTAATGAATTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTaaa                          >  2:696050/1‑141 (MQ=11)
                                                                                                                    taaagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                        >  1:652921/14‑141 (MQ=11)
                                                                                                                    taaagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                        <  1:816443/128‑1 (MQ=11)
                                                                                                                    tAAAAATTTAAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGGGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGc                        <  1:775698/141‑1 (MQ=255)
                                                                                                                     aaagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCt                       >  2:494764/13‑141 (MQ=11)
                                                                                                                      aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAAATATGTTATACCGACGAGAAAAGCTc                      >  2:243752/12‑141 (MQ=11)
                                                                                                                      aagatttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGAAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTc                      >  1:147441/12‑141 (MQ=11)
                                                                                                                       aaaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTCACTAATGATGATTATAATGTAAAACAATTACGCCTTCTGTAATAGATACCGACGAGTAAAGCTcc                     <  1:405589/141‑1 (MQ=17)
                                                                                                                        aaTTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCa                    >  1:118133/1‑141 (MQ=255)
                                                                                                                           ttaatgaaTTATAAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGAGGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGGTCCAAAg                 >  1:432105/7‑141 (MQ=14)
                                                                                                                           ttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATCATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAg                 >  2:461671/1‑141 (MQ=255)
                                                                                                                           ttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACGTTTACGCAAAAGATGTGATATACCGAGGGGTAGAGCTCCaagg                 >  1:124632/1‑139 (MQ=17)
                                                                                                                           ttGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAg                 >  1:494523/1‑141 (MQ=255)
                                                                                                                            tGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGt                >  2:508406/1‑141 (MQ=255)
                                                                                                                            tGAAAAATTATAAAGAAGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGt                >  1:496273/1‑141 (MQ=255)
                                                                                                                            aaagtaaTTATAAAGAGGGAGATATTTCATATAATCCAGAGGTGCCGAGTAATTCGGCTAAATAACAATTATCAAAGAATTATAACAATGTAAAGCATATAGTGAAAAGATAGGATATATCGACTAGTAAAGCTCCAAAGt                <  2:383480/136‑1 (MQ=9)
                                                                                                                               aaaaTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTAAGTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTAt             >  1:788688/1‑141 (MQ=255)
                                                                                                                                aaaTTATAAAGACGGAGATATTTCATATAAGCCAGAGGTGCCGAGTTATTCGGGTAAATATCAATTAACTAATGATGATTATAATGTAAAACACTTACGCAAAAGATATGATCTACAGACGAGTAAAGCTCCAAAGTTAtt            <  2:405073/141‑1 (MQ=18)
                                                                                                                                  aTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt          <  1:181142/141‑1 (MQ=255)
                                                                                                                                  aTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt          <  1:735710/141‑1 (MQ=255)
                                                                                                                                  aTTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGt          <  1:351685/141‑1 (MQ=255)
                                                                                                                                      taAAGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTGGAATGATAGTTAAGTGACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaa      >  2:591272/1‑141 (MQ=17)
                                                                                                                                       aaaGATGGAGATATATCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaaa     <  1:603068/141‑1 (MQ=255)
                                                                                                                                       aaaGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaaa     <  1:5177/141‑1 (MQ=255)
                                                                                                                                       aaaGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATCATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTaaaa     <  1:90064/141‑1 (MQ=255)
                                                                                                                                          gACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGt  <  2:773320/141‑1 (MQ=255)
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AAAAGATATCCGGTTAAAATGGTCGATAATAAAATCATTCCAACAAAAGAAATTAAAGATGAAAAAATAAAAAAAGAAATCGAAAACTTTAAGTTCTTTGTTCAATATGGCGACTTTAAAAATTTGAAAAATTATAAAGACGGAGATATTTCATATAATCCAGAGGTGCCGAGTTATTCGGCTAAATATCAATTAACTAATGATGATTATAATGTAAAACAATTACGCAAAAGATATGATATACCGACGAGTAAAGCTCCAAAGTTATTGTTAAAAGGT  >  CP000730/110511‑110789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: