Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 478,502 C→A 100% D342E (GAC→GAA USA300HOU_0457 → NADH dehydrogenase (ubiquinone)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730478,5020CA99.0% 291.7 / ‑5.9 104D342E (GAC→GAAUSA300HOU_0457NADH dehydrogenase (ubiquinone)
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base A (47/53);  total (48/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 104 total aligned reads displayed.

GTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCA  >  CP000730/478365‑478639
                                                                                                                                          |                                                                                                                                         
gTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCt                                                                                                                                        >  2:757050/1‑141 (MQ=255)
    aaTGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATg                                                                                                                                    >  1:381994/1‑141 (MQ=255)
     aTGTGCATTGGGTGTGTATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATgg                                                                                                                                   >  1:622014/1‑141 (MQ=255)
     aTGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCCTATgg                                                                                                                                   >  2:752897/1‑141 (MQ=255)
         gCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTgg                                                                                                                               <  2:424292/141‑1 (MQ=255)
            ttcggTGGAGATGCAGCAGTGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGTATCTGCTAAAGAAGCTTATGGCTGGAtt                                                                                                                            <  1:453480/138‑1 (MQ=255)
            ttGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGAtt                                                                                                                            >  1:176654/1‑141 (MQ=255)
            ttGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGAtt                                                                                                                            >  2:796726/1‑141 (MQ=255)
             tGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTg                                                                                                                           <  1:167930/141‑1 (MQ=255)
             tGGGTGTAAATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTg                                                                                                                           <  1:479595/141‑1 (MQ=255)
                gtgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCa                                                                                                                        <  2:304574/141‑1 (MQ=255)
                  gtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATg                                                                                                                      >  1:807009/1‑141 (MQ=255)
                       ttCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACAGCTATGGCTGGATTGTCATGGGACg                                                                                                                 <  2:594294/141‑1 (MQ=255)
                       ttCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACg                                                                                                                 >  1:153064/1‑141 (MQ=255)
                       ttCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACg                                                                                                                 >  1:347106/1‑141 (MQ=255)
                        tCAGCTGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgt                                                                                                                <  2:77534/141‑1 (MQ=255)
                        tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAATGGCGCTTATGGCTGGATTGTCATGGGACgt                                                                                                                <  2:384689/141‑1 (MQ=255)
                        tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgt                                                                                                                <  1:57285/141‑1 (MQ=255)
                        tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgt                                                                                                                <  2:202454/141‑1 (MQ=255)
                        tCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACgt                                                                                                                <  2:439774/141‑1 (MQ=255)
                         cagcagCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTGATGGCTGGATTGTCATGGGACggg                                                                                                               >  1:664231/1‑139 (MQ=255)
                                tactaggcATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATACTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGc                                                                                                        <  2:411057/134‑1 (MQ=255)
                                 attattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGACTGTCATGGGACGTGTATTAGCt                                                                                                       >  2:691027/1‑141 (MQ=255)
                                  ttattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTGTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCta                                                                                                      >  1:442545/1‑141 (MQ=255)
                                   tattCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCtat                                                                                                     >  1:743531/1‑141 (MQ=255)
                                     ttCATTTAATATTGCACGGTATTTTTTTTTTTTTTTTTTTTTTAAAATGGGGTTCTATCGTGGTGCGATTTATTTTTGCAATACAAGGATCTGCTTCAGAAGCTTATGGCTGGTTTGTCTTGGGTCGTTTAttggtttttt                                                                                                   >  2:419291/1‑133 (MQ=255)
                                         tttAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGt                                                                                               >  1:688628/1‑141 (MQ=255)
                                         tttAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGt                                                                                               <  1:329500/141‑1 (MQ=255)
                                              tatTGCACGGTATTCTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGTTGGATTGTCATGGGACGTGTATTAGCTATTATCGTTGCAt                                                                                          <  1:598591/141‑1 (MQ=255)
                                               atTGCACGGTTTTTTTAAAGCAAGATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGTCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAtt                                                                                         <  2:706003/141‑1 (MQ=255)
                                               atTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAtt                                                                                         >  1:192084/1‑141 (MQ=255)
                                                ttGCACGGTATTTTTAAAGCACCATTATTTTTTGTAGCAGGTTCTGGTATCGGCGTAGTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAttt                                                                                        <  1:190319/141‑1 (MQ=255)
                                                  gCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCt                                                                                      >  2:792261/1‑141 (MQ=255)
                                                  gCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCCATTATCGTGGCGTTTCt                                                                                      >  2:792316/1‑141 (MQ=255)
                                                  gCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTAACTATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTTTTAGCTATTGTCTTGGCATTTCt                                                                                      >  2:380742/1‑141 (MQ=255)
                                                    aCGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAt                                                                                    <  2:459300/141‑1 (MQ=255)
                                                     cGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTggtctttgcatt                                                                                   >  2:367373/1‑131 (MQ=255)
                                                     cGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAtt                                                                                   >  2:35361/1‑141 (MQ=255)
                                                     cGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTAtt                                                                                   >  2:389302/1‑141 (MQ=255)
                                                         ttttctAAAGCAATATTATTTTTATAATCAGGTTCTATCGTGAAGCGATTTAGTATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg                                                                               <  2:352719/140‑1 (MQ=255)
                                                         aTTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTgg                                                                               >  2:566564/1‑141 (MQ=255)
                                                              tAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGag                                                                          <  1:32828/141‑1 (MQ=255)
                                                                ttttttttttttttttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGAATCTGCTAAAGGAGCTTTTGGCTTGATTGTTATGTGATGTGTAATAGCTATTTTCGTGGCATTTCTATTTGTGATGagta                                                                        >  2:748138/12‑141 (MQ=255)
                                                                aaGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGagta                                                                        >  2:178329/1‑141 (MQ=255)
                                                                    aaCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGa                                                                    <  2:711580/141‑1 (MQ=255)
                                                                    aaCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGa                                                                    <  1:124149/141‑1 (MQ=255)
                                                                     aCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCGTTTCTATTTTGGTTGAGTAGTGAc                                                                   >  1:340289/1‑141 (MQ=255)
                                                                     aCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGAc                                                                   >  1:536217/1‑141 (MQ=255)
                                                                      cATTATTTTTACAATCAGGTTCAATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACa                                                                  <  1:835990/141‑1 (MQ=255)
                                                                       attattTTTACAATCAGGTTATATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGATGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTAGCGTGGCATTTCTATTTTGGATGAGTAGTGACAg                                                                 <  1:250060/141‑1 (MQ=255)
                                                                         tattTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGGTGAGTAGTGACAGaa                                                               >  2:50580/1‑141 (MQ=255)
                                                                            ttttACAATCAGGTTCTATCGTGAAGCGATTCACTATTCCAAAACACGCATCTGCTAAAGAAGCTTATGGCTGGATTGTACTGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGCTAAGTAGTGCCAGAAGTg                                                            >  2:649148/1‑141 (MQ=255)
                                                                            ttttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTg                                                            >  2:832507/1‑141 (MQ=255)
                                                                            ttttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTg                                                            <  1:683264/141‑1 (MQ=255)
                                                                             tttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGc                                                           >  1:59585/1‑141 (MQ=255)
                                                                              ttACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGATGGATTGTCATGGGACGTGTATTAGCAATTATCGTGGCAGTTCTATTTTGGGTGAGTAGTGACAGAAGTGCa                                                          >  2:417286/1‑141 (MQ=255)
                                                                               tACAATGAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCat                                                         <  1:309085/141‑1 (MQ=255)
                                                                                atactCAGGTTCTATCGTGAAGCGATTTAATATTCCAAAACAAGCATCTGCGAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCAGTTCTATTTTGGATGAGTAGTGACAGAAGTGCata                                                        <  1:657332/137‑1 (MQ=255)
                                                                                   aTCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGGTGAGTAGTGACAGAAGTGCATATGa                                                     >  2:446840/1‑141 (MQ=255)
                                                                                    tCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGaa                                                    <  1:595885/141‑1 (MQ=255)
                                                                                     cAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAg                                                   >  1:258333/1‑141 (MQ=255)
                                                                                     cAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAg                                                   >  2:818358/1‑141 (MQ=255)
                                                                                      aGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgg                                                  >  2:243706/1‑140 (MQ=255)
                                                                                        gTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgtgt                                                <  2:661501/141‑1 (MQ=255)
                                                                                          tCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTa                                              <  1:796726/141‑1 (MQ=255)
                                                                                             ttCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGt                                           <  1:275309/140‑1 (MQ=255)
                                                                                             aTCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGt                                           >  1:30611/1‑141 (MQ=255)
                                                                                              tCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTg                                          <  2:802884/141‑1 (MQ=255)
                                                                                                 tGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGGGACAGAAGCGCATATGAAGGTTTAAGTGCAc                                       >  1:284191/1‑141 (MQ=255)
                                                                                                  gAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACt                                      <  2:622014/141‑1 (MQ=255)
                                                                                                     gCGATTGAATATTCCAAAACAAGCATCTGCTAAAGAAGCTGATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAt                                   <  1:792316/141‑1 (MQ=255)
                                                                                                     gCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAt                                   >  2:784223/1‑141 (MQ=255)
                                                                                                     gCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAt                                   <  1:792261/141‑1 (MQ=255)
                                                                                                     gCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCAt                                   <  1:121449/141‑1 (MQ=255)
                                                                                                       gATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTc                                 >  1:452580/1‑141 (MQ=255)
                                                                                                         aTTCAATATTCCTAAAACAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCt                                <  1:396627/141‑1 (MQ=255)
                                                                                                         aTTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCt                                >  2:737799/1‑141 (MQ=255)
                                                                                                            cAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTCACAGTAGTAGGTACAGCAGTTCATATGAAGTGTTAAGTGCACTCATTCTAgg                             <  2:381994/141‑1 (MQ=255)
                                                                                                             aaTATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGa                            <  1:35361/141‑1 (MQ=255)
                                                                                                             aaTATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGGAGTTACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGa                            <  1:367373/141‑1 (MQ=255)
                                                                                                               tatTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATg                          <  1:26380/141‑1 (MQ=255)
                                                                                                                atTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATgg                         <  1:691027/141‑1 (MQ=255)
                                                                                                                   ccAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCATGGCATTTCTATTTTGGATGAGTCGTGACCCAAGTTCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCa                      >  1:154986/1‑141 (MQ=255)
                                                                                                                      aaaCAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTa                   <  2:638011/141‑1 (MQ=255)
                                                                                                                      aaaCAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTa                   <  2:229665/141‑1 (MQ=255)
                                                                                                                       atctagccacTGCTAAAGCAGCTTATGGCTGGTTTGTCATGGGAGGTGTATTAGCTATTATCGTGGCATTTCTTTTTTGGATGTGTAGTGACAGAAGTGCATATGAACTGTTAAGTGCTCTCATTCTAGGATGGTCATTAc                  <  2:220768/132‑1 (MQ=255)
                                                                                                                       aatatggtagcgCTAAAGAATCTTATGGTGGGATTGTCATGGGACGTGTATTAGCTATTAGTGTGGGATTTCTATTTTGGATGTGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTAc                  <  2:190177/130‑1 (MQ=255)
                                                                                                                        aCAAGCATCTGCTAAAGAAGCTTATGGCGGGATTGGCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACt                 <  1:99352/141‑1 (MQ=255)
                                                                                                                         cAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACtt                <  2:738165/141‑1 (MQ=255)
                                                                                                                          aggCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTg               <  2:560384/139‑1 (MQ=255)
                                                                                                                          aaGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCCTTCTAGGATGGTCATTACGTg               >  1:11459/1‑141 (MQ=255)
                                                                                                                           aGCATCTGCTAAAGAAGCTTACGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGt              <  2:192084/141‑1 (MQ=255)
                                                                                                                             cATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTAt            <  2:347106/141‑1 (MQ=255)
                                                                                                                                cTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCtt         >  1:708848/1‑141 (MQ=255)
                                                                                                                                 tGCTTAAGAAGCGTATGGCTGGATTGTCAGGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATAGGAAGTGTTAAGTGCACGCATTCTAGGATGGCCATTACTTGTATCTTg        <  1:417286/141‑1 (MQ=255)
                                                                                                                                 tGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTg        <  1:832507/141‑1 (MQ=255)
                                                                                                                                   cTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGa      <  1:537006/141‑1 (MQ=255)
                                                                                                                                   cTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGa      <  1:537539/141‑1 (MQ=255)
                                                                                                                                       aGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCa  <  2:310484/141‑1 (MQ=255)
                                                                                                                                       aGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCa  <  2:807009/141‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                         
GTTCAATGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGGAATCA  >  CP000730/478365‑478639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: