Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 707,909 A→T 100% M1M (TTG→ATG) † USA300HOU_0653 ← ABC superfamily ATP binding cassette transporter, ABC protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730707,9090AT100.0% 174.0 / ‑6.5 59M1M (TTG→ATG) †USA300HOU_0653ABC superfamily ATP binding cassette transporter, ABC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base T (29/29);  minor base G (0/1);  total (29/30)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAA  >  CP000730/707771‑708045
                                                                                                                                          |                                                                                                                                        
aCTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGATATTCAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATAc                                                                                                                                        >  1:649276/1‑141 (MQ=255)
   tGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTGCTGGTATCGATAAGGAAATGTTTTTAAGTTCAGGCTTATTACCTAAAAGCAGATTTAAATCTTTTGTTTCTAACATACttt                                                                                                                                     >  1:23401/1‑141 (MQ=255)
        aGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAc                                                                                                                                <  1:654773/141‑1 (MQ=255)
          ggAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATAcct                                                                                                                              >  2:244112/1‑141 (MQ=255)
           gAAGATTTACCAGCACCATTCGGGCCCATGATACAAATGGTTTCGCCTCTTGCTGTGTTCGATAAGGTAATGTTTATAAGGACATGCTCATTAGCTACGAACTGATTTAACTCTTCTGTTTCTAACATACGGTTATAcctc                                                                                                                             <  2:224388/141‑1 (MQ=255)
              gATTTCCCAGCACCATTCGGGTCCATGATACCAAGTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTa                                                                                                                          <  1:188924/141‑1 (MQ=255)
                        cACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTGAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCCAACATACGTTTATACCTCCTAATTAAAAGtt                                                                                                                <  1:75445/141‑1 (MQ=255)
                         aCCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGttt                                                                                                               <  2:300040/141‑1 (MQ=255)
                                  gCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAAcc                                                                                                      <  2:666448/141‑1 (MQ=255)
                                    cgatgtttctaaTTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCta                                                                                                    <  2:806702/131‑1 (MQ=255)
                                    ccATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCta                                                                                                    >  1:374215/1‑141 (MQ=255)
                                      aTGATACCCATTATTTCGCCGCGTAGTGGTATCGATAAGGAACTGTTTTCAAGTACATGCTTATTACCTAAAAAAAGATTTAAATCTTTTGTTCCTAACATCCGAGTATACCTCCTAATTAAAAGTTTAGGCTCACCaaat                                                                                                  >  2:268814/1‑141 (MQ=255)
                                           aCCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTaattaatt                                                                                             >  1:520070/1‑141 (MQ=255)
                                             cAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTACAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGt                                                                                           <  1:564074/141‑1 (MQ=255)
                                             cAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATCCATTTCACGGAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGt                                                                                           <  1:608417/141‑1 (MQ=255)
                                                ttattTCGCCGCGTACTGGGATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGtata                                                                                        <  2:836063/141‑1 (MQ=255)
                                                  attTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTataat                                                                                      >  2:177713/1‑141 (MQ=255)
                                                   tttCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTataata                                                                                     <  2:157173/141‑1 (MQ=255)
                                                     tCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTATGTACTTGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATaaa                                                                                   >  2:763419/1‑141 (MQ=255)
                                                     tCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATaaa                                                                                   >  2:684463/1‑141 (MQ=255)
                                                     tCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATaaa                                                                                   >  1:46551/1‑141 (MQ=255)
                                                      cgccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATaaaa                                                                                  >  2:369190/1‑140 (MQ=255)
                                                      cgccgcGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAAc                                                                                  <  1:489674/141‑1 (MQ=255)
                                                          gcgTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTgag                                                                              >  1:308572/1‑141 (MQ=255)
                                                           cgTACTGGTATCGATAAGGAAGTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTTATTTGCAGTGTAAGCTGACCTAATTAATTGTATAATAAACTgaga                                                                             >  1:434098/1‑141 (MQ=255)
                                                            gTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAACAACCTgccaa                                                                            >  2:752430/1‑137 (MQ=255)
                                                             tACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAat                                                                           >  1:481591/1‑141 (MQ=255)
                                                               cTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAatat                                                                         >  2:636666/1‑141 (MQ=255)
                                                                  gTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAAGAAACTGAGAATATTTa                                                                      <  1:417747/141‑1 (MQ=255)
                                                                    aTCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATc                                                                    <  1:233567/141‑1 (MQ=255)
                                                                            ggAAATGTTTTTTAGTTCATGCGTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                                                            <  1:189218/141‑1 (MQ=255)
                                                                            ggAAATGTTTTTAAGTACATGCCTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAg                                                            <  1:688089/141‑1 (MQ=255)
                                                                               aaTGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaa                                                         <  2:453794/141‑1 (MQ=255)
                                                                                aTGTTTTTAAGGACATGCTTATTACCTAAAAATAGATTTAAATCTTTTGTTTCTACCATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTaaa                                                        <  2:515831/141‑1 (MQ=255)
                                                                                 tGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAt                                                       >  2:592882/1‑141 (MQ=255)
                                                                                 tGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAAt                                                       >  2:505096/1‑141 (MQ=255)
                                                                                    ttttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCg                                                    >  1:734048/1‑141 (MQ=255)
                                                                                     tttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAGGTAAATTCGt                                                   >  2:627459/1‑141 (MQ=255)
                                                                                     tttAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACATCCTAATTAAAAGTTTAGGCGAACCTAATTAATTGTATAATAAACTGATAATATTTATCATGTCAAGTAAATCCGt                                                   >  1:192046/1‑141 (MQ=255)
                                                                                         agcaCATGCTTATTACCTAAAAACATATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGata                                               <  2:248218/138‑1 (MQ=255)
                                                                                          gTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatat                                              <  2:319021/141‑1 (MQ=255)
                                                                                            aCATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataa                                            <  2:692613/141‑1 (MQ=255)
                                                                                               tGCTTATTACCTAAAAACAAATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATGAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGatataatat                                         <  2:649276/141‑1 (MQ=255)
                                                                                                   tattaCCTAAAAACAGATTGAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGaa                                     >  1:625625/1‑140 (MQ=255)
                                                                                                       acttAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatg                                 <  2:517821/138‑1 (MQ=255)
                                                                                                       aCCTAAAAACAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATGAACAatg                                 >  2:837977/1‑141 (MQ=255)
                                                                                                            aaaaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAatgtatgt                            <  2:520070/141‑1 (MQ=255)
                                                                                                              aaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATCCCTCCTAATTAAAAGTTCAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGa                          <  1:734801/141‑1 (MQ=255)
                                                                                                              aaCAGATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGa                          >  2:182377/1‑141 (MQ=255)
                                                                                                                cAGATTTAGATCTTTTGTTTCTAAGCGTCTTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAgg                        <  2:625625/141‑1 (MQ=255)
                                                                                                                  gATTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTg                      >  1:766074/1‑141 (MQ=255)
                                                                                                                   aTTTAAATCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGAGATATAATATAGACAATGTATGTGAGGTGa                     >  2:596533/1‑141 (MQ=255)
                                                                                                                      tAAATCTTTTGTTTCTTACATATGATTATACCTCCTAATTAAAAGTTTAGGCTCACCTAATTAATTGTATAATAAACTTAGAATATTTATCATGTCAAGTAAATTCGTGATTTAATATAGACAATGTATGTGAGGTGAAAg                  <  2:710414/141‑1 (MQ=255)
                                                                                                                        aaTCTTTTGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTa                <  2:23401/141‑1 (MQ=255)
                                                                                                                            ttttGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGtt            >  2:122351/1‑141 (MQ=255)
                                                                                                                              ttGTTTCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTaa          >  1:808712/1‑141 (MQ=255)
                                                                                                                                ttttCTAACATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACt        <  2:567760/140‑1 (MQ=255)
                                                                                                                                  ttCTAACATACGTTTATACCACCTAATTAAAAGTTTAGACTAACCTAACTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTTATATAATAAAGACAATGTATGTGAGGTGAAAGTATGTTAACTga      <  2:249976/141‑1 (MQ=255)
                                                                                                                                      aaCATACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTgaagaa  >  1:16116/1‑141 (MQ=255)
                                                                                                                                          |                                                                                                                                        
ACTTGATAAGGGAAGATTTACCAGCACCATTCGGGCCCATGATACCAATTATTTCGCCGCGTACTGGTATCGATAAGGAAATGTTTTTAAGTACATGCTTATTACCTAAAAACAGATTTAAATCTTTTGTTTCTAACAAACGTTTATACCTCCTAATTAAAAGTTTAGGCTAACCTAATTAATTGTATAATAAACTGAGAATATTTATCATGTCAAGTAAATTCGTGATATAATATAGACAATGTATGTGAGGTGAAAGTATGTTAACTGAAGAA  >  CP000730/707771‑708045

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: