Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 35,876 C→T 50.6% intergenic (+107/+1) USA300HOU_0025 → / ← USA300HOU_0026 hypothetical protein/IS431mec transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073035,8760CT50.6% 58.6 / 176.0 132intergenic (+107/+1)USA300HOU_0025/USA300HOU_0026hypothetical protein/IS431mec transposase
Reads supporting (aligned to +/- strand):  ref base C (23/42);  new base T (24/43);  total (47/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

Only 100 of 136 total aligned reads displayed.

AGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACC  >  CP000730/35736‑36016
                                                                                                                                            |                                                                                                                                            
aGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAAAAAAATTGTGATCTCATAAAAATAGAAGGTTCTGAAACAAAGAAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGc                                                                                                                                              <  2:738458/141‑1 (MQ=38)
  cTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACGAATTTATCATGTCAGTGTTCGCtt                                                                                                                                            <  1:610640/141‑1 (MQ=255)
       cAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACtt                                                                                                                                       <  1:358008/141‑1 (MQ=255)
         gTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGc                                                                                                                                     >  2:147429/1‑141 (MQ=255)
              aaGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCa                                                                                                                                >  1:22430/1‑141 (MQ=255)
                     tGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCt                                                                                                                         >  1:655806/1‑141 (MQ=255)
                       gCGAAGAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTaa                                                                                                                       <  2:110178/141‑1 (MQ=255)
                       gCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTaa                                                                                                                       >  1:170303/1‑141 (MQ=255)
                       gCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTaa                                                                                                                       >  2:270981/1‑141 (MQ=255)
                               tGATTACTTGAACAACAGCGGCGCAAAGATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTgg                                                                                                               >  2:181572/1‑141 (MQ=17)
                                 aTTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCa                                                                                                             <  1:671244/141‑1 (MQ=255)
                                     cTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAATTAAAAAAATATAGCTAACCACTAATTTATCAGGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                                                                                         <  2:72996/141‑1 (MQ=255)
                                     cTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTGATCATGTCAGTGTTCGCTTAACTTGCTAGCGTGATGCTAATTTCGTGGCATGGc                                                                                                         <  2:562144/141‑1 (MQ=255)
                                              gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTTAGTGTTCGCTTAACTTGCTCGCATGATGATAATTTAGTGGCATGGCGAAAATCCg                                                                                                >  1:507400/1‑141 (MQ=255)
                                              gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                                                                                >  1:551358/1‑141 (MQ=255)
                                              aagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCg                                                                                                <  2:710303/140‑1 (MQ=255)
                                                   aaaataaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGAt                                                                                           >  1:821036/1‑141 (MQ=255)
                                                      acaaaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAGATCCGTAGATCTGaa                                                                                      >  2:345561/15‑141 (MQ=11)
                                                       caaaagagtattCTACTAAAAATGATGGTTCTGTTGCGAAGTAAAAAATATAGCTAATCACTCATATAGCAGGTCAGTGTTCGTTTAACTTGCGAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                     <  2:517678/130‑1 (MQ=11)
                                                        aaaatTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAAGTTGCTACAATCATGCTAATTTCGTGGCATGCCGAAAATCCGTAGATCTGaa                                                                                      >  2:11244/1‑141 (MQ=17)
                                                         aaagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATACTAATTTCTTGGCAGCGTCAAAATCCGTAGATCTGAagag                                                                                   <  1:616312/130‑1 (MQ=2)
                                                          aagagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                                                                                  <  1:833569/131‑1 (MQ=11)
                                                          aagagtattttaataaAAATGATGGTTCTGTCGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAgaga                                                                                  <  1:445313/131‑1 (MQ=11)
                                                           agggtattgtaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGGGTTCGTTTAACTTGCTAGCATGATGCTAATTGCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  2:193729/132‑1 (MQ=11)
                                                           agcgtattttaataaAAATGATGGTTCTGTTGCAAAGAAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  2:706920/132‑1 (MQ=11)
                                                           agagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:778908/132‑1 (MQ=11)
                                                            gagtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAcc                                                                                <  2:537940/133‑1 (MQ=11)
                                                             tgtgCTCTGATAAAAATAGAAGGTTCTGTTACAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  2:839706/141‑1 (MQ=255)
                                                             tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  2:222094/141‑1 (MQ=255)
                                                             tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACGTTCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  2:760745/141‑1 (MQ=255)
                                                             tgtgATATAGTAAAAATAGCCGGTTATGAGGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                 <  1:393895/141‑1 (MQ=11)
                                                             agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                               <  1:375906/134‑1 (MQ=11)
                                                             agtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                               <  1:413813/134‑1 (MQ=11)
                                                              gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                              >  1:496512/7‑141 (MQ=11)
                                                              gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                              <  1:733475/135‑1 (MQ=11)
                                                              gtattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                              <  1:698599/135‑1 (MQ=11)
                                                               tgATATAATAAAAATAGAAGGTTCTGTGGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                               <  2:463726/141‑1 (MQ=255)
                                                               tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATTGCGAAAATCCGTAGATCTGAAGAGACCTgg                                                                             >  2:668475/6‑140 (MQ=11)
                                                               tattttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                             <  1:797411/136‑1 (MQ=11)
                                                                taacTAATAAAAATAGAAGGTTCTATTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                              <  2:149111/138‑1 (MQ=255)
                                                                attttaataaAAATGATTGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                            <  2:483250/137‑1 (MQ=11)
                                                                 ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCGCTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                           >  2:278790/4‑141 (MQ=11)
                                                                 ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGATCTGAAGAGACCTGCgg                                                                           >  1:341215/4‑141 (MQ=11)
                                                                 ttttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                           >  1:492142/4‑141 (MQ=11)
                                                                 ttttaataaAAATGATGGTTCTGCTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                           <  1:152758/138‑1 (MQ=11)
                                                                 aTCTATTAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                             <  2:609559/141‑1 (MQ=255)
                                                                 aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                             <  1:102870/141‑1 (MQ=255)
                                                                 aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                             <  1:206777/141‑1 (MQ=255)
                                                                  tttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAACCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCTACAATCCGTAGATCTGAAGAGGCGTGCggg                                                                          >  2:534774/3‑140 (MQ=11)
                                                                   ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCGGTAGAACTGAAGAGACCGGCGGtt                                                                         >  2:496364/2‑141 (MQ=11)
                                                                   ttaataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                         <  2:49125/140‑1 (MQ=11)
                                                                    taataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                          <  2:655806/141‑1 (MQ=40)
                                                                    taataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                                        <  1:427491/141‑1 (MQ=11)
                                                                     aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCt                                                                       >  2:127452/1‑141 (MQ=11)
                                                                     aataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTATTCGTTTAACTTGCTAGCATGATGCTAATTTCGTCGCATGCCGAAAATCCGTAGATCTGAAGAGCCCTCGGGTTCt                                                                       >  2:174776/1‑141 (MQ=11)
                                                                      ataaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtt                                                                      >  2:21499/1‑141 (MQ=11)
                                                                       taaAAATGATGGTTCTGTTGCAAAGTAAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCttt                                                                     <  1:84329/141‑1 (MQ=11)
                                                                       aaaaaaaTGAAGGTTCTGATGCAAAGTAAAAAATATAGCTAATCACTAATATAACATATAAGAGAACGTTTAACTTGCTAACATGTTGCTAATACCGAGGACTTGCGAAAATCCGTAGATCTGAAGAGAACTGCGGTTCtt                                                                      >  2:250293/2‑141 (MQ=2)
                                                                          aaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGTTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtttt                                                                    <  1:166336/141‑1 (MQ=25)
                                                                             tAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCCGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat                                                                 <  1:679145/141‑1 (MQ=17)
                                                                             tAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtat                                                                 <  2:263973/141‑1 (MQ=32)
                                                                               gAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCAAGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtctat                                                               >  1:478299/1‑141 (MQ=14)
                                                                                    ttatGTTGCAAAGTAAAAAATATAGGTAATCACTATTATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCg                                                         <  2:259477/138‑1 (MQ=1)
                                                                                                               cacccagttatgtATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAATGt                               <  1:225176/129‑1 (MQ=1)
                                                                                                                aatcACTAATATATCATGTCAGTGTTCGTTTAACTTGCGAGCATGAAGCTCATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTa                              <  1:110147/138‑1 (MQ=1)
                                                                                                                aatcACTAATATATAATGTCAGTGTTCGTTTAACTTGTTAGTATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTa                              <  1:57181/138‑1 (MQ=1)
                                                                                                                          ttttCATGTCAGTGTTCGCTTATCTTGCTGGCATGATGCTAATTTTGTGGAATGGCGAACATCCGTAGATCTGAAGAGACCTGAGGTTTTTTGTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTg                    <  2:374443/138‑1 (MQ=1)
                                                                                                                             tcttgccAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTAt                 <  1:73442/135‑1 (MQ=0)
                                                                                                                                   ccgtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc           <  1:700862/139‑1 (MQ=11)
                                                                                                                                   cAGTGTTCGTTTAACTTGCTAGCATGATGTTAATTTCGTGCCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc           <  2:449155/141‑1 (MQ=2)
                                                                                                                                   cAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATAc           <  1:643657/141‑1 (MQ=11)
                                                                                                                                   cAGTGTTCGTTTAACGTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc           <  2:824557/141‑1 (MQ=2)
                                                                                                                                   cAGGGTTCGTTTAACGTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAAGCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc           <  1:408115/141‑1 (MQ=11)
                                                                                                                                    tgtgtTCGCTTAACTTGCTGGCAGGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACt          <  1:727050/140‑1 (MQ=9)
                                                                                                                                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGCGACCTACGGTTCTTTTTATATAGCGTGAGAATAAATTCAATACATTTGAAAGTATTCTTTGCCGTATTGATACtt         >  1:190894/1‑141 (MQ=11)
                                                                                                                                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt         >  1:51387/1‑141 (MQ=11)
                                                                                                                                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGAGACtt         >  1:67873/1‑141 (MQ=11)
                                                                                                                                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         >  1:709009/1‑141 (MQ=12)
                                                                                                                                     gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATGCTTTGCTGTATTGATACtt         >  2:352513/1‑141 (MQ=12)
                                                                                                                                     gtgtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt         >  1:411387/1‑141 (MQ=17)
                                                                                                                                      tgtTCGTTTAACTTGCTAGCATGATGCTAGTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATGCATTCAATACCTTTTAAATTATTATTTTCCGTATTGCTACttt        >  2:29432/1‑141 (MQ=11)
                                                                                                                                      tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt        >  1:236628/1‑141 (MQ=11)
                                                                                                                                       gtTCGTTTAACCTGCTAGCATGATGCTAATTTCGTCGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTCAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg       <  1:344226/141‑1 (MQ=11)
                                                                                                                                       gtTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg       >  2:445339/1‑141 (MQ=32)
                                                                                                                                       ggtCGTTTAACTTGGTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTg       <  2:334567/139‑1 (MQ=11)
                                                                                                                                        ttCGTTTGACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATAAACTTAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  1:368056/141‑1 (MQ=11)
                                                                                                                                        ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa      <  1:19576/141‑1 (MQ=255)
                                                                                                                                        ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGa      <  1:383263/141‑1 (MQ=255)
                                                                                                                                        ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATTGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  1:310734/141‑1 (MQ=255)
                                                                                                                                        ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGTGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  1:280561/141‑1 (MQ=17)
                                                                                                                                        ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      >  2:828351/1‑141 (MQ=17)
                                                                                                                                        ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  2:698976/141‑1 (MQ=17)
                                                                                                                                        ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa      <  2:800174/141‑1 (MQ=17)
                                                                                                                                         tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGAt     <  1:497644/141‑1 (MQ=255)
                                                                                                                                         tCGCTTACCTTGCAAGCATGATGCTAATTTCGTGGAATGGCGAAAAACCGTAGATCGGAAACGACGTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt     <  2:192935/141‑1 (MQ=11)
                                                                                                                                          cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa    >  1:629438/1‑141 (MQ=17)
                                                                                                                                          cGCTTAACTGGCTGGCATGATGCTAATCTCGTGGCATGTCGAAAATCCGTCGAGCTGAAGAGACCTGCGGTTCTTGTTATATAGATCGGAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa    <  2:411558/141‑1 (MQ=11)
                                                                                                                                           gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc   <  2:794607/141‑1 (MQ=17)
                                                                                                                                            cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAATTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCTTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc  <  1:11244/141‑1 (MQ=17)
                                                                                                                                            cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc  <  2:624269/141‑1 (MQ=17)
                                                                                                                                            |                                                                                                                                            
AGCTATTCAGTATAAAGTTTTTGGCGAAAAATGATTACTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACC  >  CP000730/35736‑36016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: