Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,744,473 C→T 100% A67T (GCT→ACT)  apt ← adenine phosphoribosyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,744,4730CT100.0% 145.9 / ‑6.5 52A67T (GCT→ACT) aptadenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (21/30);  minor base A (1/0);  total (22/30)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTA  >  CP000730/1744337‑1744600
                                                                                                                                        |                                                                                                                               
tGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACCGGTGCAAAGACAATCCACCTAGAGTAAGTTACa                                                                                                                             >  1:117488/1‑141 (MQ=255)
 gACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAg                                                                                                                            >  2:288949/1‑141 (MQ=255)
  aCCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAgg                                                                                                                           >  2:643425/1‑141 (MQ=255)
        tctAATTGCCTTTTTTTTCATTGTTACAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAAGCCCCATTGAATAAGTTACAGGACAGcc                                                                                                                     <  1:12536/139‑1 (MQ=255)
                  tCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGCCAGCCAAGGATaaac                                                                                                           >  2:279746/1‑140 (MQ=255)
                      tgtgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAc                                                                                                       <  1:187678/141‑1 (MQ=255)
                        tgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCAcgc                                                                                                     <  1:4137/141‑1 (MQ=255)
                        tgCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAAGGATAAAGCCAcgc                                                                                                     >  2:603241/1‑141 (MQ=255)
                         gCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACGGCCAATGATAAAGCCAcgcc                                                                                                    >  1:333097/1‑140 (MQ=255)
                         gCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGccctgcg                                                                                                    >  1:102984/1‑136 (MQ=255)
                          cATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACgcgc                                                                                                   <  1:181435/141‑1 (MQ=255)
                                  aaaCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTc                                                                                           <  2:451219/141‑1 (MQ=255)
                                    aCATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCt                                                                                         >  2:260409/1‑141 (MQ=255)
                                                      aGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCACCAtct                                                                       >  1:643889/1‑141 (MQ=255)
                                                      aGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTAACACGATATCAACAtcc                                                                       >  2:35081/1‑140 (MQ=255)
                                                        gTCATACTCATAACGAATGACTTCAGGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATctct                                                                     >  1:419344/1‑141 (MQ=255)
                                                        gTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGATACAGGACAGCCAATGATAAAGCCACGCGCTTCAGATCCTACAACCACATctctttctct                                                                     >  2:85913/1‑132 (MQ=255)
                                                         tCATACTAATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTg                                                                    <  2:114966/141‑1 (MQ=255)
                                                          cATACTCCTAACTAATGACTTCACGAGGGAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt                                                                   >  1:121781/1‑141 (MQ=255)
                                                          cATACTCCTAACCAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACCGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCTCGCGCTTCAGGTCCTACGACGATATCAACATCTCTGt                                                                   <  2:119805/141‑1 (MQ=255)
                                                          cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGt                                                                   <  1:717369/141‑1 (MQ=255)
                                                          cATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGGCCTACAACGCTATCAACATCTCTGt                                                                   <  1:419414/141‑1 (MQ=255)
                                                           aTACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTc                                                                  <  2:81968/141‑1 (MQ=255)
                                                              cTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCttt                                                               <  2:465655/141‑1 (MQ=255)
                                                               ccATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCtttt                                                              <  2:670373/140‑1 (MQ=255)
                                                                cATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCACCATCTCTGTCttttt                                                             >  1:783877/1‑140 (MQ=255)
                                                                     cGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTAt                                                        <  2:199844/141‑1 (MQ=255)
                                                                        aTGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCt                                                     <  1:643425/141‑1 (MQ=255)
                                                                                ccGAGTTAATTTCCCTTCTTTCCGAACACGTGCACAGCCAATCCCCATTGAATAAGTTACAGGACCGCCAATGATCAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTTTGTCTTTTGCGTATTCTACAAtttt                                             <  2:123281/140‑1 (MQ=255)
                                                                                aCGAGGTAATTTCCCTTCTTTTCTACCAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAAtttt                                             <  1:748777/141‑1 (MQ=255)
                                                                                 cGAGGTACTTCGCCTTCTTTTCTTACCGGTGCAATGCCAAGGTCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCTTGCGCTACAGGTCCTTCAACGATATCAACATCTCTGTCTTTTGCGTATTCTTCAATTTTa                                            <  2:322199/141‑1 (MQ=255)
                                                                                   aGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACCCGCTTACGGTCCTACAACGATATCAACATCGATGCCTTTCACCTATTCTTCAACTTtctc                                          >  2:577565/1‑141 (MQ=255)
                                                                                      tAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGt                                       <  1:562202/141‑1 (MQ=255)
                                                                                        aTTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTg                                     <  1:798902/141‑1 (MQ=255)
                                                                                        aTTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTg                                     <  1:288949/141‑1 (MQ=255)
                                                                                          ttCCCTTTTTTTCTATCTGCTGAAAAGCCATTCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCTCGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCa                                   <  2:28162/141‑1 (MQ=255)
                                                                                           tCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCAt                                  >  1:484401/1‑141 (MQ=255)
                                                                                           tCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGGCTTTTGCGTATTCTACAGTTTTATCTGTTGCAt                                  >  1:484370/1‑141 (MQ=255)
                                                                                              cttcttTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGc                               >  2:407490/1‑141 (MQ=255)
                                                                                                    ttCTAACAGGTGCAAAGCCAATCACCACTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATg                         >  2:593477/1‑141 (MQ=255)
                                                                                                       tAACAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCtt                      <  1:703818/141‑1 (MQ=255)
                                                                                                          cAGGTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCAc                   >  2:3157/1‑141 (MQ=255)
                                                                                                             gTGCAAAGCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAt                <  1:260409/141‑1 (MQ=255)
                                                                                                              tGCAAATCCAATCCCCATTGAATAATTTACAGTACAGCCAATGATAAAGCCACGCGCTTCAGTTCCTACAACGATATCAACATCTCTTTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCAtt               <  2:499227/141‑1 (MQ=255)
                                                                                                                  acgCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGCCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc           <  1:485802/139‑1 (MQ=255)
                                                                                                                  aaGCCAATCCCCATTGAATAAGTTACAGGACAGCAAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATcc           <  1:673695/141‑1 (MQ=255)
                                                                                                                    gCCAATCCCCATTGAATAAGTTACGGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCAt         >  1:536169/1‑141 (MQ=255)
                                                                                                                    gCCAATCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCCCGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCAt         <  1:646744/141‑1 (MQ=255)
                                                                                                                    cccaacgccgatgcaaTAAGTTACAGGACAGACAATGATAAAGCCACGCGCTTCACTTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCAt         <  2:207267/127‑1 (MQ=255)
                                                                                                                       aaTCCCCATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCCCCATTATCCATact      >  2:257590/1‑139 (MQ=255)
                                                                                                                          ccccATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgt   <  1:683578/141‑1 (MQ=255)
                                                                                                                           cccATTGAATAAGTTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTgta  <  2:307276/141‑1 (MQ=255)
                                                                                                                                        |                                                                                                                               
TGACCTGGTTTAATTGCATCTTTGTGCATTGTTAAAACATTTGTACCATATTCTAGGTCATACTCATAACGAATGACTTCACGAGGTAATTTCCCTTCTTTTCTAACAGGTGCAAAGCCAATCCCCATTGAATAAGCTACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTA  >  CP000730/1744337‑1744600

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑