BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,940	G→A	30.5%	R204R (CGC→CGT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,940	0	G	A	30.5%	424.9 / 301.2	415	R204R (CGC→CGT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (124/163); new base A (65/61); total (190/225)
Fisher's exact test for biased strand distribution p-value = 1.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-01

Only 100 of 424 total aligned reads displayed.

GTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35798‑36080

gtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                                              <  1:698599/135‑1 (MQ=11)

gtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                                              <  1:733475/135‑1 (MQ=11)

gtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTg                                                                                                                                              >  1:496512/7‑141 (MQ=11)

 tattgtaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCAAATCACTAATATATCATGTCAGTGTTCGTTTAACTTACTAGCATGATGCTAATTTCGTGGCATAGCGAAACTCCGTAAAACTGCATAGCCCTGc                                                                                                                                             >  2:437670/6‑141 (MQ=2)

  attttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCg                                                                                                                                            <  2:206346/137‑1 (MQ=11)

   ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGATCTGAAGAGACCTGCgg                                                                                                                                           >  1:341215/4‑141 (MQ=11)

   ttttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCgg                                                                                                                                           >  1:492142/4‑141 (MQ=11)

   aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                                             <  1:206777/141‑1 (MQ=255)

     ttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                                                                                         <  2:49125/140‑1 (MQ=11)

       aataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTATTCGTTTAACTTGCTAGCATGATGCTAATTTCGTCGCATGCCGAAAATCCGTAGATCTGAAGAGCCCTCGGGTTCt                                                                                                                                       >  2:174776/1‑141 (MQ=11)

            aaaTAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGTTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCtttt                                                                                                                                    <  1:166336/141‑1 (MQ=25)

                tgAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACGAATTTGTCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTtata                                                                                                                                <  2:643114/140‑1 (MQ=14)

                      ttatGTTGCAAAGT‑AAAAAATATAGGTAATCACTATTATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCg                                                                                                                         <  2:259477/138‑1 (MQ=1)

                                                 cacccagttatgtATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAATGt                                                                                               <  1:225176/129‑1 (MQ=1)

                                                                     cAGTGTTCGTTTAACGTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                           <  2:824557/141‑1 (MQ=2)

                                                                     cAGGGTTCGTTTAACGTGCTAGCAGGATGCTAATTTCGTGGCATGGCGAAAAGCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                           <  1:408115/141‑1 (MQ=11)

                                                                       gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGCGACCTACGGTTCTTTTTATATAGCGTGAGAATAAATTCAATACATTTGAAAGTATTCTTTGCCGTATTGATACtt                                                                         >  1:190894/1‑141 (MQ=11)

                                                                       gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACtt                                                                         >  1:709009/1‑141 (MQ=12)

                                                                        tgtTCGTTTAACTTGCTAGCATGATGCTAGTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATGCATTCAATACCTTTTAAATTATTATTTTCCGTATTGCTACttt                                                                        >  2:29432/1‑141 (MQ=11)

                                                                        tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTCTTGATACttt                                                                        >  1:391896/1‑141 (MQ=12)

                                                                         gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                       >  2:372585/1‑141 (MQ=12)

                                                                          ttCGTTTGACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATAAACTTAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                      <  1:368056/141‑1 (MQ=11)

                                                                          ttCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATTGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                      <  1:310734/141‑1 (MQ=255)

                                                                          ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                      <  1:507513/141‑1 (MQ=17)

                                                                          ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                      <  1:360668/141‑1 (MQ=17)

                                                                            cgtttAACTTGCTAGCATGATGCTAATTTCGGGGCATGGCGAAAATGCGTAGATCTGAAGGGACCTGCGGTTGTTTTTATATAGAGCGTAGATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGTTACTTAGATa                                                                    >  2:415503/4‑141 (MQ=11)

                                                                            cgtgtAACTTACTAGCATGATGCTAATTTCGTGGCGGGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTGAATACATTCAATGCCTTTTAAAGTATTCTTTGCCGTATTGCTACTTTGATa                                                                    >  2:383292/5‑141 (MQ=11)

                                                                            cGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATa                                                                    >  1:629438/1‑141 (MQ=17)

                                                                              cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAATTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCTTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                  <  1:11244/141‑1 (MQ=17)

                                                                               ttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCt                                                                 <  2:84801/141‑1 (MQ=17)

                                                                                 aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAGCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTg                                                               <  2:714150/141‑1 (MQ=17)

                                                                                   cTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtt                                                             >  2:811310/1‑140 (MQ=17)

                                                                                    ttGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtct                                                            >  2:286038/1‑141 (MQ=255)

                                                                                     tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                           >  1:90462/1‑141 (MQ=17)

                                                                                      tcTAGGATGATGCTAATGTCGTGGCATGGCGAAAATCCGTAGGTGTGAAGAGACCTGCGGTTCTTTTTATATAGGTCGTAAATACACTCAATACCTTTTAAAGTATTCTTGGCTGTATTGAGACTTTGATACCTTGtcttt                                                          <  2:336240/140‑1 (MQ=11)

                                                                                      gCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATGTCTTGtcttt                                                          >  1:562702/1‑141 (MQ=11)

                                                                                       cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttc                                                         >  2:13581/1‑141 (MQ=11)

                                                                                       cTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATCCCTTTTAAAGTATTCTTTGCTGTATTGATACTTGGATACCTTGtctttc                                                         <  2:829952/141‑1 (MQ=12)

                                                                                        tAGCATGGTGCTACTTTCGTGGCATGGCGAAAATCCGTAGATCTTAATAGACCTGCGGTTCTTTTTATCTAGCGCGTAAATTCCCTCACTACCTTTTACAGTATTCTTTGCTGTAGTGATACTTTGATACCTTGtctttct                                                        <  2:762453/141‑1 (MQ=11)

                                                                                          gCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTa                                                      <  2:227348/141‑1 (MQ=255)

                                                                                          gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                      >  1:301938/1‑141 (MQ=17)

                                                                                          gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                      >  1:43511/1‑141 (MQ=17)

                                                                                          gCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTa                                                      >  1:457499/1‑141 (MQ=17)

                                                                                            atgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATGTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATCCCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGCTACCTTGTCTTTCTTACt                                                    >  1:469012/1‑141 (MQ=255)

                                                                                            atgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAATATTCTTTGCTGTATTGATACTTTGATACCATGTCTTTCTTACt                                                    >  2:604133/1‑141 (MQ=16)

                                                                                            atgatgCCAATTTCGTGGCATGGCGAAAATCCGTAGATCGGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACt                                                    <  2:514774/141‑1 (MQ=21)

                                                                                             tgatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACTTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTTTTACtt                                                   >  2:629887/1‑141 (MQ=255)

                                                                                               atgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTa                                                 >  1:282514/1‑141 (MQ=255)

                                                                                               atgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTa                                                 >  1:802369/1‑141 (MQ=11)

                                                                                               atgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTa                                                 >  1:534175/1‑141 (MQ=21)

                                                                                                 gCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGCTATCTTGTCTTTCTTACTTTAat                                               >  2:267163/1‑141 (MQ=11)

                                                                                                 gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAat                                               <  2:770770/141‑1 (MQ=25)

                                                                                                  cTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAata                                              <  1:670128/141‑1 (MQ=11)

                                                                                                  cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                                              <  1:270981/141‑1 (MQ=35)

                                                                                                  cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAatt                                              >  2:534445/1‑140 (MQ=21)

                                                                                                   tAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTTATACCTTGTCTTTCTTACTTTAatat                                             >  2:318363/1‑141 (MQ=21)

                                                                                                    aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATg                                            >  2:449614/1‑141 (MQ=11)

                                                                                                        tCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACgg                                        >  1:249403/1‑141 (MQ=255)

                                                                                                          gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTGTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGATGGTg                                      <  2:579135/141‑1 (MQ=11)

                                                                                                          gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATTACGTTg                                      >  2:806145/1‑141 (MQ=255)

                                                                                                           gggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATGGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                     <  2:11477/140‑1 (MQ=255)

                                                                                                           aggCATGACGAAAATCCGTAGAACTGAAGAGACCGGCGGTTCTTTTTATATAGAGCGAAAATACACTCAATACCTTTTAAAGTATGCTTTGCTGTATTGATACTTTGATACATTGTCTTTCTTACTTTAATATGACGGTGa                                     >  2:746443/2‑141 (MQ=11)

                                                                                                            ggCCTGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    <  1:804845/141‑1 (MQ=255)

                                                                                                            ggCATGGCGAAAAGCCGTAGATCTGAATAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    <  2:507400/141‑1 (MQ=255)

                                                                                                               aTGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCtt                                 <  2:193932/141‑1 (MQ=255)

                                                                                                                     aaaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                           <  1:267021/141‑1 (MQ=255)

                                                                                                                      aaaTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                          >  1:481529/1‑141 (MQ=37)

                                                                                                                       aaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTCGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATg                         <  2:524598/141‑1 (MQ=255)

                                                                                                                        aTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                        >  1:684462/1‑141 (MQ=38)

                                                                                                                        aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGa                        <  1:722454/141‑1 (MQ=255)

                                                                                                                          ccGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATGGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                      <  1:329520/141‑1 (MQ=255)

                                                                                                                          ccGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAgg                      >  2:481516/1‑141 (MQ=255)

                                                                                                                           cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATAAATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTACTACATTGACTTTCTTACTTTAATTAGCAGGTGAACTTGATTACTGAGGt                     >  2:357814/1‑141 (MQ=11)

                                                                                                                           cGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     <  2:561508/141‑1 (MQ=255)

                                                                                                                            gTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGtt                    >  2:539823/1‑141 (MQ=255)

                                                                                                                            gTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTGAATATGACGGTGATCTTGCTCAATGAGGtt                    >  1:748827/1‑141 (MQ=255)

                                                                                                                               gATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                 <  1:147429/141‑1 (MQ=39)

                                                                                                                               gATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                 <  2:613906/141‑1 (MQ=38)

                                                                                                                               gATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                 <  2:243160/141‑1 (MQ=255)

                                                                                                                               gATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                 <  1:13581/141‑1 (MQ=255)

                                                                                                                                aTCTGCAGAGTCTTGCGGTTCTTTTTAGAGAGAGCGTAAATACACTCAATAACTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTc                <  2:656441/141‑1 (MQ=11)

                                                                                                                                  cttaaGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  1:1805/138‑1 (MQ=255)

                                                                                                                                  cTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAg              <  1:645011/141‑1 (MQ=255)

                                                                                                                                   tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGCTACTCTTTGACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             >  1:822656/1‑141 (MQ=11)

                                                                                                                                   tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             <  1:574991/141‑1 (MQ=255)

                                                                                                                                   tGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             <  1:772566/141‑1 (MQ=38)

                                                                                                                                   tGAAGAGACCTACGGTTCTTTTTACATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATATTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             <  2:102036/141‑1 (MQ=11)

                                                                                                                                   ctatgtgaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTCTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGa             <  2:158777/135‑1 (MQ=11)

                                                                                                                                     aaGAGACCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata           <  2:298098/141‑1 (MQ=255)

                                                                                                                                      agagACCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGatat          <  2:674006/141‑1 (MQ=255)

                                                                                                                                        agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATACTTTGCTGTATTGATAATTTGATACATTCTCTTTCTTACTTTAATATAACGATGATCTTGCTCAATGAGGTTATACAGATAttt        >  2:695406/1‑141 (MQ=11)

                                                                                                                                        agaCCTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt        >  2:79365/1‑141 (MQ=37)

                                                                                                                                        agaCCTACGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTACTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAttt        >  2:731950/1‑141 (MQ=255)

                                                                                                                                         gaCCTACGGTTCTTTTTATATAGTGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc       <  1:603426/141‑1 (MQ=255)

                                                                                                                                           ccTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa     <  1:796379/141‑1 (MQ=255)

                                                                                                                                           ccTGCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa     <  2:222676/141‑1 (MQ=255)

                                                                                                                                             tGCGGTTTTTTTTATATAGAGCGTAAATACATTCAATATCTTTTAAAGCATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGCCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATg   <  2:404631/141‑1 (MQ=255)

                                                                                                                                             tGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATg   <  1:100883/141‑1 (MQ=39)

                                                                                                                                              gCGGTTCTTTTTATATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAAGATGACGGTGATCTTGCTCAATGAGGTTATTGAGATATTTCGATGt  <  1:448481/141‑1 (MQ=255)

                                                                                                                                              gCGGTTCTTTTTAGATAGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTACTATTACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGt  <  2:343659/141‑1 (MQ=255)

GTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35798‑36080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`