BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	1,929,867	G→T	68.0%	intergenic (‑950/‑218)	USA300HOU_1794 ← / → USA300HOU_1796	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	1,929,867	0	G	T	68.0%	160.7 / 130.6	158	intergenic (‑950/‑218)	USA300HOU_1794/USA300HOU_1796	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (22/29); new base T (49/58); total (71/87)
Fisher's exact test for biased strand distribution p-value = 8.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 158 total aligned reads displayed.

GTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCA  >  CP000730/1929728‑1930004

gtAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑t                                                                                                                                            >  1:658926/1‑141 (MQ=255)

            aTCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTCtttt                                                                                                                              >  2:819802/1‑141 (MQ=255)

                  cAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCGGAGAGGGGGTGTG‑TAA‑G‑G‑T‑GT‑TGCTTTTTTTCGTTca                                                                                                                        >  1:594507/1‑141 (MQ=255)

                        gAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCAcatcat                                                                                                                  >  1:698946/1‑141 (MQ=255)

                             atatAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCAc                                                                                                             >  2:327764/1‑141 (MQ=255)

                              tataACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGAAcc                                                                                                            >  2:791623/1‑141 (MQ=255)

                                      aTCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGtcatca                                                                                                    <  1:138099/141‑1 (MQ=255)

                                          atcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGc                                                                                               >  2:192086/15‑141 (MQ=255)

                                           tcggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCCCCAGCCATCAGTGCCt                                                                                              >  1:42930/14‑141 (MQ=255)

                                             ggaagtagatatgCTTTTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                            <  2:433576/130‑1 (MQ=255)

                                             ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGGGGAGGTGCA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                            <  2:154340/130‑1 (MQ=255)

                                             ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                            <  1:803573/130‑1 (MQ=255)

                                             ggaagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgt                                                                                            <  1:488992/130‑1 (MQ=255)

                                             gCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtg                                                                                             <  1:167235/141‑1 (MQ=255)

                                               gagttgatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                          <  2:321725/132‑1 (MQ=255)

                                               aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                          <  2:558803/132‑1 (MQ=255)

                                               aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                          <  1:797647/132‑1 (MQ=255)

                                               aagtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑TGGC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGc                                                                                          >  1:337450/10‑141 (MQ=255)

                                               aaGTGACTGTGCTTGGATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCtgtg                                                                                           <  1:59458/141‑1 (MQ=255)

                                                agtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                         <  2:673894/133‑2 (MQ=255)

                                                agtagatatgCTTGTATATTCACAGATGGGATAATAATAGAGTCGCCTATCTCTCAGGCGGCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGct                                                                                         <  2:26005/133‑2 (MQ=255)

                                                 gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                        >  1:760732/8‑139 (MQ=255)

                                                 gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                        >  1:349963/8‑139 (MQ=255)

                                                 gtagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTACGGCGACTAATT‑‑ACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctg                                                                                        <  1:823123/134‑3 (MQ=255)

                                                  tagatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgt                                                                                       <  1:550427/135‑4 (MQ=255)

                                                   agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAGTT‑‑GCCGACGATAGACGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTAGTTCGCATCATAGCCCCCGTCAGCAGTGGCTgcgctgct                                                                                      >  2:219394/6‑137 (MQ=255)

                                                   agatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATAAGTGGCTGTGctgtt                                                                                      >  2:352146/6‑137 (MQ=255)

                                                    gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                     >  1:208871/5‑136 (MQ=255)

                                                    gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                     <  1:297781/137‑6 (MQ=255)

                                                    gatatgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGctgttg                                                                                     >  2:542625/5‑136 (MQ=255)

                                                     cttttcTTGTATATTTGGCGATGATATAATAGTCGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTg                                                                                     <  2:784971/136‑1 (MQ=255)

                                                       gtgCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCg                                                                                   <  1:632163/141‑1 (MQ=255)

                                                       atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                  <  2:738597/140‑1 (MQ=255)

                                                       atgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGt                                                                                  >  2:601971/2‑141 (MQ=255)

                                                        tgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGta                                                                                 <  1:479830/141‑2 (MQ=255)

                                                        tgCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCACTTC‑A‑T‑T‑GC‑AAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGta                                                                                 >  1:822430/1‑140 (MQ=255)

                                                         gCTTGTATATTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtat                                                                                <  1:550332/141‑3 (MQ=255)

                                                           ttGTATATTCACAGATGGTGTAATAATAGAGTCGCCTATCTCTCAGGCGTCAACT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtatta                                                                              >  2:511428/1‑140 (MQ=255)

                                                              tataTTCACAGATGGTATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGtattatac                                                                           <  2:356748/141‑8 (MQ=255)

                                                                tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCAAATCATAGCACCAGTCATCAGTGCCTGTGCTGTTGCGtattatactt                                                                         >  2:298755/9‑135 (MQ=255)

                                                                tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                                         >  2:55213/9‑141 (MQ=255)

                                                                tattcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑T‑T‑TACTAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACtt                                                                         >  2:605023/9‑141 (MQ=255)

                                                                tatTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCt                                                                          <  2:255223/141‑1 (MQ=255)

                                                                  ttcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAt                                                                       >  2:810716/7‑141 (MQ=255)

                                                                   tcacagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTAtt                                                                      <  1:361057/136‑1 (MQ=255)

                                                                      cagatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGc                                                                   <  1:413275/139‑1 (MQ=255)

                                                                       aggtggtataATAAGAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                  <  2:514985/135‑1 (MQ=255)

                                                                       agatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCt                                                                  <  1:786025/140‑1 (MQ=255)

                                                                        gatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATCATTCTTGGCtt                                                                 >  2:752379/1‑140 (MQ=255)

                                                                        gatgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCt                                                                  <  1:619346/141‑1 (MQ=255)

                                                                        aatggtATAATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACGTCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCtt                                                                 <  2:50584/140‑2 (MQ=255)

                                                                         atgatATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTa                                                                 <  1:490140/141‑1 (MQ=255)

                                                                          tggtataATACTAGATGCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                               <  2:756122/138‑4 (MQ=255)

                                                                          tgatATAATAGTAGAGTCGCCTATATCTCAAGCTTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTaa                                                                >  2:347823/1‑141 (MQ=255)

                                                                          gggtataATAATAGAGTCCCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCttag                                                               <  1:711134/138‑4 (MQ=255)

                                                                          gggtataAGAGTAGAGTCGCCTATCTTTCGTGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGTACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAg                                                               <  2:498392/138‑1 (MQ=255)

                                                                           ggtataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                              <  1:361531/139‑1 (MQ=255)

                                                                           ggtataATAATAGAGTCGCCTATCCCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCTCATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGt                                                              <  1:241552/139‑1 (MQ=255)

                                                                            gtataATAGGGGGGGCGCCTATCTCTCAGGCGTGAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt                                                              <  2:191874/140‑1 (MQ=255)

                                                                            atatAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt                                                              >  2:41776/1‑141 (MQ=255)

                                                                            atatAATAGTAGAGTCGACTCTCTAAAGGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGt                                                              >  2:385095/1‑141 (MQ=255)

                                                                             tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                            >  2:115692/1‑140 (MQ=255)

                                                                             tataATAATAGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaa                                                            >  2:365784/1‑140 (MQ=255)

                                                                              ataataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTaaa                                                           >  1:156826/1‑139 (MQ=255)

                                                                                aataatAGAGTTGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑CTT‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                         >  1:307755/1‑141 (MQ=255)

                                                                                aataatAGAGTGGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACg                                                         <  2:239390/141‑1 (MQ=255)

                                                                                 ataataGAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGc                                                        <  1:810716/141‑1 (MQ=255)

                                                                                   aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                      <  1:111478/139‑1 (MQ=255)

                                                                                   aatagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaa                                                      >  2:650488/3‑141 (MQ=255)

                                                                                    atatagTCCCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                                     <  1:107199/137‑2 (MQ=255)

                                                                                    atagAGTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaac                                                     >  2:396951/2‑140 (MQ=255)

                                                                                      agagTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATa                                                    >  2:836573/1‑141 (MQ=255)

                                                                                       gagTCGCCGATGTGTCAGGCTGCAATCGAGGCGCAGAGGGGAGGTGTA‑TAA‑G‑G‑G‑GA‑TGCTTATTTTCGTTCACATCATAGCTCCAGTCATTAGTGGCTGTGCCAGTGCGTTTTTGTCTGATGGGCTAAGTAGACGCAATAc                                                   <  2:171833/141‑1 (MQ=255)

                                                                                        agTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaat                                                 >  2:198806/1‑136 (MQ=255)

                                                                                         gTCGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCAACAaaaa                                                >  1:13125/1‑141 (MQ=255)

                                                                                          tCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACaaa                                                >  2:314793/1‑141 (MQ=255)

                                                                                           cGCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataat                                              >  1:580713/1‑133 (MQ=255)

                                                                                            gCCTGTCTCTCAGGCGTCAATT‑‑GGCGAGGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCCTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                             <  2:133413/141‑10 (MQ=255)

                                                                                            gCCTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                             <  2:568671/141‑10 (MQ=255)

                                                                                            gCCTATCTCTCAGGCGTAAATT‑‑GACGAAGAGAGGAGGTGCA‑TTA‑C‑TTT‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatt                                             <  2:641506/141‑10 (MQ=255)

                                                                                             ccTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataatta                                            <  1:814002/141‑11 (MQ=255)

                                                                                              cTATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAGTCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaa                                           <  2:571334/141‑12 (MQ=255)

                                                                                               tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaaa                                          >  2:284760/1‑129 (MQ=255)

                                                                                               tATCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGATGTGCTGTTGCTTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                          >  1:781601/1‑129 (MQ=255)

                                                                                               tATCTCTCAGGCGTCAA‑‑TAGACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaat                                          <  1:735220/141‑13 (MQ=255)

                                                                                                ttctctcAGGCGTCAATTTAGACGCAGAGCGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGt                                          <  1:230311/140‑1 (MQ=255)

                                                                                                aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TCATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                         >  2:131690/1‑128 (MQ=255)

                                                                                                aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCATTAC‑T‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                         >  2:198995/1‑128 (MQ=255)

                                                                                                aTCTCTCAGGCGTCAATT‑‑GACGAAGAGAGGAGGTGCA‑TTACT‑T‑T‑GC‑TAATCATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCTGTTGCGTATTATACTTATTGGCTTAGTAAACGCaacaaataattaata                                         >  1:417073/1‑128 (MQ=255)

                                                                                                  ctctcAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑A‑G‑T‑GA‑TGCTTATCTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCCATCCAAAATACGTgg                                        >  2:139871/1‑140 (MQ=255)

                                                                                                      cAGGCGTTGATTTAGACGAAGAATTTAGTTTTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACata                                    <  2:625527/141‑1 (MQ=255)

                                                                                                        ggCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTCTTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGAGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACAtata                                  >  1:788125/1‑141 (MQ=255)

                                                                                                                 ttAGCCGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCa                         <  1:331499/141‑1 (MQ=255)

                                                                                                                 ttAGCCGCAGAGAGGAGGTGTA‑CAA‑G‑G‑T‑GC‑TGCTTATTTTCGAAAGAGTCATCGTTTATGGCCCCGATCGCTGTGCCATTGCGTTTTGTGCTAGTTTTCCAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCa                         <  2:804414/141‑1 (MQ=255)

                                                                                                                       gCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATaaaa                   <  2:576682/141‑1 (MQ=255)

                                                                                                                              ggaggTGTA‑TAA‑G‑G‑T‑GA‑TGCTGATTTTCGTTCGCATCATAGAACCAGTAATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCt            <  2:610102/141‑1 (MQ=255)

                                                                                                                                aggTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTTTTTTTTGTTTAGTAGACGCAATACAAAATGGGTGACATATACCCGCGCAAGTACAAATCCCCTcc          >  2:787829/1‑140 (MQ=255)

                                                                                                                                 tgtgtA‑TAA‑C‑G‑T‑TA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCAc         <  1:52103/140‑1 (MQ=255)

                                                                                                                                     ta‑taA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTAcc     <  2:83127/141‑1 (MQ=255)

                                                                                                                                         aa‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTCCCGCa  >  2:22551/1‑141 (MQ=255)

GTAGACGATTCAATCTATCAGCTAGAATCATATAACTTATCAGAAGCAAGTGACTGTGCTTGTATATTTGCCGATGATATAATAGTAGAGTCGCCTATCTCTCAGGCGTCAATTTAGACGCAGAGAGGAGGTGTA‑TAA‑G‑G‑T‑GA‑TGCTTATTTTCGTTCACATCATAGCACCAGTCATCAGTGGCTGTGCCATTGCGTTTTTTTCTTATTGGCTAAGTAGACGCAATACAAAATAGGTGACATATAGCCGCACCAATAAAAATCCCCTCACTACCGCA  >  CP000730/1929728‑1930004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`