Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,532,298 A→T 100% T15T (ACA→ACT USA300HOU_2392 → FemAB family peptidoglycan biosynthesis protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,532,2980AT100.0% 282.5 / NA 88T15T (ACA→ACTUSA300HOU_2392FemAB family peptidoglycan biosynthesis protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (23/65);  total (23/65)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA  >  CP000730/2532161‑2532433
                                                                                                                                         |                                                                                                                                       
tgtgTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTactaca                                                                                                                                      <  2:123805/141‑1 (MQ=255)
  tgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACaaa                                                                                                                                    >  1:38716/1‑141 (MQ=255)
  tgtATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACaaa                                                                                                                                    >  1:47069/1‑141 (MQ=255)
    tttttGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc                                                                                                                                  <  2:691205/139‑1 (MQ=255)
    tATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGc                                                                                                                                  <  2:535336/141‑1 (MQ=255)
      tttGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAt                                                                                                                                >  2:242004/1‑141 (MQ=255)
        tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttt                                                                                                                              <  1:736251/141‑1 (MQ=255)
        tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttt                                                                                                                              <  1:386277/141‑1 (MQ=255)
        tGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttt                                                                                                                              <  1:343444/141‑1 (MQ=255)
         gTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTTAATTCTGATGAGTTCAATGCGTTTACTACTAACCAtttt                                                                                                                             >  2:316291/1‑141 (MQ=255)
         gTAATCAGTTTTTGATAAAATAAAAAAATAGCAGTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGTATGGGATAAAATGAACTTTGTAACGTTGCCTGCGGATGTGTTGAATGCGTTTACTACAAAGAAtttt                                                                                                                             >  2:277308/1‑141 (MQ=255)
          tAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCAttttt                                                                                                                            <  1:385265/141‑1 (MQ=255)
           aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTc                                                                                                                           >  1:705206/1‑141 (MQ=255)
           aaTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAAAGAGGAATAGGATAAAATGAACTTTGTAATGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCACTTTTc                                                                                                                           >  2:791275/1‑141 (MQ=255)
            aTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTca                                                                                                                          <  2:708164/141‑1 (MQ=255)
            aTCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAAAGAGGGATGGGAGAAAATGAACTTTATAACGTTGACTTCGGATGAGTTCAATGCGTATACTACAAAGCATATTTca                                                                                                                          >  2:504987/1‑141 (MQ=255)
             acattttctGATAAAAGAAAAAAAGTGCATTATTAATAAATTCAAAAATTATAGTTCAAATCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTcac                                                                                                                         <  2:164149/137‑1 (MQ=255)
                    ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                  <  2:662267/141‑1 (MQ=255)
                    ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                  <  2:731975/141‑1 (MQ=255)
                    ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                  <  2:355889/141‑1 (MQ=255)
                    ttGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                  <  1:195448/141‑1 (MQ=255)
                    ttGAAAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacac                                                                                                                  <  2:335214/141‑1 (MQ=255)
                     tGATAAAATAAAAAAATAGCATTATTAATATAGTCACAAAATATATGTGAATTCAGAGAGGGATGGGATAAAATGAACTTTTTCACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCCTTTTTCACATTacaca                                                                                                                 <  2:801645/141‑1 (MQ=255)
                     tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTaccca                                                                                                                 >  1:295298/1‑141 (MQ=255)
                     tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTacaca                                                                                                                 >  1:205418/1‑141 (MQ=255)
                     tGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAAATTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCCAATTacaca                                                                                                                 >  2:114819/1‑141 (MQ=255)
                       ataaaataaaAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTAcacaca                                                                                                               <  1:685997/141‑1 (MQ=255)
                        aaaaataaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACaa                                                                                                              <  2:834430/140‑1 (MQ=255)
                           aataaaaAAATAGCATTATTAATAAATTCAAAAACTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATCAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCa                                                                                                           <  1:343595/141‑1 (MQ=255)
                           aaaaaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTACAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGCTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCa                                                                                                           <  2:264654/138‑1 (MQ=255)
                            ataaaaAAAGAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAg                                                                                                          <  1:84286/141‑1 (MQ=255)
                             taaaaAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTGGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc                                                                                                         <  1:825749/141‑1 (MQ=255)
                             aaaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAAGTCAGAGAGGGATGGTATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGc                                                                                                         <  1:209474/140‑1 (MQ=255)
                              aaaaaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAAGGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCt                                                                                                        <  2:614505/141‑1 (MQ=255)
                                 aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCCGCTagt                                                                                                     <  2:306218/141‑3 (MQ=255)
                                 aaaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAtt                                                                                                     <  2:205239/141‑1 (MQ=255)
                                  aaaTAGCATTATTAATAAATTCAAAAATTATAGTTCAAATCAGCTATGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTc                                                                                                    <  1:781043/141‑1 (MQ=255)
                                   aaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCa                                                                                                   <  2:690457/141‑1 (MQ=255)
                                   aaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCa                                                                                                   <  1:565482/141‑1 (MQ=255)
                                   aaTAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCa                                                                                                   <  1:344341/141‑1 (MQ=255)
                                           tattaATAAATTCAAAAATTATAGTTCAATTCATAGAGGGATGGGATAAAATGAACTGTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATc                                                                                           <  2:545865/141‑1 (MQ=255)
                                              taacaaATTGAAAAATTATAGGTCAATTCTGAGAGGGATGGTATAAAAGGAACTTTGTAACGGTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTGCACATTACACACAATCAGCTATTCATTACAATCATa                                                                                        <  2:784735/141‑1 (MQ=255)
                                               aataaATTCAAAAATTATATTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATag                                                                                       <  2:796676/141‑1 (MQ=255)
                                               aataaATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATag                                                                                       <  1:93241/141‑1 (MQ=255)
                                                          aaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaa                                                                            >  2:77969/1‑141 (MQ=255)
                                                          aaaTTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTaa                                                                            >  1:263600/1‑141 (MQ=255)
                                                                taGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACACAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                                      <  1:449022/141‑1 (MQ=255)
                                                                taGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                                      <  2:351013/141‑1 (MQ=255)
                                                                taGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                                      <  1:162073/141‑1 (MQ=255)
                                                                taGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                                      <  2:516118/141‑1 (MQ=255)
                                                                taGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                                      <  1:693205/141‑1 (MQ=255)
                                                                aaGTTCAAGTCAGAGAGGGATTCGATAAAATGAACTTTGTAACGTCGACTTCGGATGAGTTCAATGCGTTTACTACAAAGTCTTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCg                                                                      <  2:174813/140‑1 (MQ=255)
                                                                  gTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGAt                                                                    <  1:314525/141‑1 (MQ=255)
                                                                         tCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATc                                                             <  1:669384/141‑1 (MQ=255)
                                                                         tCAGAGAGGGATGGGAGAAAAGGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATc                                                             <  1:407204/141‑1 (MQ=255)
                                                                           agagagGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCtt                                                           <  1:307821/141‑1 (MQ=255)
                                                                               ggggatgtggtAAAATGGACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAg                                                       <  2:393924/140‑1 (MQ=255)
                                                                               agGGATGGGATAAAATGAACTTTGTAACGTTGAGTTCGGATGAGTTCAAGTCGTTTACTACAAAGCATTGTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAg                                                       <  2:576113/141‑1 (MQ=255)
                                                                                 ggatgggatAAAATGAACTTTGTGACGTTGTCTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAggg                                                     <  2:232785/141‑1 (MQ=255)
                                                                                 ggatgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAggg                                                     >  1:115967/1‑141 (MQ=255)
                                                                                   gtgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                   <  2:30239/140‑1 (MQ=255)
                                                                                   atgggatAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGt                                                   <  1:462577/141‑1 (MQ=255)
                                                                                         ttcaaTGCACTTTTTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTAATCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGa                                             <  1:493537/138‑1 (MQ=255)
                                                                                         aaaaaTGAAATTTGTAACGATGACTTCGGATGAGTTCAATGCGTTCACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCTATGTGCATCTTGTAGGGGTTAAAGa                                             <  2:693592/140‑1 (MQ=255)
                                                                                           aaaTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                           <  2:567233/141‑1 (MQ=255)
                                                                                           aaaTGAACGTTGTAACGTTCACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATg                                           <  2:190229/141‑1 (MQ=255)
                                                                                             aTGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCATATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGAc                                         <  1:670493/141‑1 (MQ=255)
                                                                                              tGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACa                                        <  2:560171/141‑1 (MQ=255)
                                                                                                      gTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCaa                                <  2:738225/141‑1 (MQ=255)
                                                                                                      gTAACGTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCaa                                >  1:586789/1‑141 (MQ=255)
                                                                                                      gTAACGTGGACTCCGGACGAGTTCAATGCGTTTACTACAAATCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCaa                                <  1:587020/141‑1 (MQ=255)
                                                                                                        aacttcgACTTCGGATGAGTTCAATGAGTTTACTACAAATCGTATTTGATCTTCATTAGAATCAGGTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCGTGTAGGGGTTAAAGATGACAATGGTCAAGt                              <  1:784833/135‑1 (MQ=255)
                                                                                                           gTTGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGAt                           >  1:46969/1‑141 (MQ=255)
                                                                                                             tGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTg                         <  2:88260/141‑1 (MQ=255)
                                                                                                             tGACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTg                         <  2:274316/141‑1 (MQ=255)
                                                                                                              gACTTCGGATGAGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGc                        >  1:292851/1‑141 (MQ=255)
                                                                                                                cttagagtGAGTTCAATTCTTTTACTACAAAGTATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAg                      <  1:271823/134‑1 (MQ=255)
                                                                                                                cTTCGGATGCGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCGATTCGTTACAATCATAGAGTTGATTTAAAAGGAGATGTGCATCTTGTAGGTGTTAAAGATGACAATGGTCTAGTGATTGCAg                      >  2:190725/1‑141 (MQ=255)
                                                                                                                   cGGATGAGTTCAATGCGTTTACTACAACGCATTTTTCACCTTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCTGTTTGTATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                   <  1:696849/141‑1 (MQ=255)
                                                                                                                   cGGATGAGTTCAATACGTTTACTACAAAGCATTTTTCACATTACACAAAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATATTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGag                   <  2:403467/141‑2 (MQ=255)
                                                                                                                   cGGATCAGTTCAATGCGTTTACTAAAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTTATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGAt                   <  2:225931/141‑1 (MQ=255)
                                                                                                                         aGTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAt             >  2:606511/1‑141 (MQ=255)
                                                                                                                          gTTCAATGCGTTTACTACACACCCTGCCGCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAtt            >  2:384900/1‑141 (MQ=255)
                                                                                                                          gTTCAATGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTAtt            <  1:593132/141‑1 (MQ=255)
                                                                                                                            tCAATGCGTTTACTACTATGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGTGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGa          <  1:504987/141‑1 (MQ=255)
                                                                                                                             cAATGCGTTTACTACAAAGAATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGCTTGCAGGATGCTTATTgca         >  2:152263/1‑139 (MQ=255)
                                                                                                                                tGCGTTTACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGa      >  2:637929/1‑141 (MQ=255)
                                                                                                                                    tttACTATAAATCATTTTTCACATTACACACAAGCAGTTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCAGTTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgca  <  1:666387/141‑1 (MQ=255)
                                                                                                                                    tttACTACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAgca  >  1:730226/1‑141 (MQ=255)
                                                                                                                                         |                                                                                                                                       
TGTGTATTTGTAATCAGTTTTTGATAAAATAAAAAAATAGCATTATTAATAAATTCAAAAATTATAGTTCAATTCAGAGAGGGATGGGATAAAATGAACTTTGTAACGTTGACTTCGGATGAGTTCAATGCGTTTACAACAAAGCATTTTTCACATTACACACAATCAGCTATTCATTACAATCATAGAGTTGATTTAAAAGGCGATGTGCATCTTGTAGGGGTTAAAGATGACAATGGTCAAGTGATTGCAGGATGCTTATTGACAGAAGCA  >  CP000730/2532161‑2532433

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: