Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,657,572 G→T 10.1% intergenic (‑555/‑127) USA300HOU_2510 ← / → USA300HOU_2511 MarR family transcriptional regulator/possible dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,657,5720GT10.1% 192.7 / 2.0 78intergenic (‑555/‑127)USA300HOU_2510/USA300HOU_2511MarR family transcriptional regulator/possible dioxygenase
Reads supporting (aligned to +/- strand):  ref base G (29/38);  new base T (7/3);  total (36/42)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCTCGAGAAAATAATTTGAAAATTATTGCCTCATGTTCATTTGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTATAGAAAAATAGAAAAAGAGGCGATGCATAATGGCATTTCACGA  >  CP000730/2657432‑2657712
                                                                                                                                            |                                                                                                                                            
cGCTCGAGAAAATAATTTGAAAATTATTGCCTCATGTTCATTTGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCg                                                                                                                                              >  2:120549/1‑141 (MQ=255)
  ctggagaAAATAATTTGAAAATTATTGCCTCATGTTCATTTGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCa                                                                                                                                            <  1:753576/138‑1 (MQ=255)
            taatTTGAAAATTATTGCCTCATGTTCATTTGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATaaa                                                                                                                                  <  1:8882/141‑1 (MQ=255)
                tttaaaaTTATTGCCTCATGTTCATTTGCAAAACATATGTTAGAAAAAGAAGATTCATATCAATATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTg                                                                                                                              <  2:52867/138‑1 (MQ=255)
                ttGAAAATTATTGACTCATGTTCATCTGCCAAACATCTGTTAGAAAAAGCCACTCCTTATGAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCAGCGCTTTTTTGTTCGCATCAATATAAAATTg                                                                                                                              <  1:397424/141‑1 (MQ=255)
                  gAAAATTATTGCCTCATGTTCATTTGTCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCa                                                                                                                            <  1:147568/141‑1 (MQ=255)
                   aaaaTTATTGCCTCATGTTCATTTGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCaa                                                                                                                           <  2:795485/141‑1 (MQ=255)
                    aaaTTATTGCCTCATGTTCATTTGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATATATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAGATTGGaaa                                                                                                                          >  2:245878/1‑141 (MQ=255)
                     aaTTATGGCCTCGTGTTTATTTGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAAGTTTGCGATGGTGCAACTGTACCAGCTCTTTTTTGTTCGCATCAATATAAAATTGCaaaa                                                                                                                         <  2:6422/141‑1 (MQ=255)
                        tattGCCTCATGTTCATTTGCCAAACTTATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCTTCGCTTCTTTGTTCGCATCAATATACAATTCCaacccga                                                                                                                      >  1:723977/1‑136 (MQ=255)
                         attGCCTCATGTTCATTTGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGAc                                                                                                                     >  1:479964/1‑141 (MQ=255)
                          ttGCCTCATGTTCATTTGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACt                                                                                                                    >  2:577423/1‑141 (MQ=255)
                          ttGCCTCATGTTCATTTGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACt                                                                                                                    >  2:673528/1‑141 (MQ=255)
                                    ttCATTTGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTg                                                                                                          <  1:550821/141‑1 (MQ=255)
                                       aTTTGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATCAAATTGCAAAACGACTAGTTATTTTGCAt                                                                                                       <  2:51566/141‑1 (MQ=255)
                                        tttGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCTCGTTTTTGTTCCCATCAACACAAAACTGCAAACCGACGAGTTATTTTGCAtt                                                                                                      <  1:438491/141‑1 (MQ=255)
                                        tttGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCAtt                                                                                                      <  2:394436/141‑1 (MQ=255)
                                            ccAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCaa                                                                                                  >  1:238389/1‑141 (MQ=255)
                                            ccAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCaa                                                                                                  <  2:611886/141‑1 (MQ=255)
                                                aCATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACtt                                                                                              >  2:762286/1‑141 (MQ=255)
                                                    atGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATg                                                                                          >  1:515680/1‑141 (MQ=255)
                                                     tGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGc                                                                                         >  1:734530/1‑141 (MQ=255)
                                                          gAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCa                                                                                    <  1:344313/141‑1 (MQ=255)
                                                          gAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCa                                                                                    <  2:238389/141‑1 (MQ=255)
                                                           aaaaaGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGCCTAGTTATTTTGCATTTCAAACTTTATGccctctt                                                                                   >  2:206749/1‑135 (MQ=255)
                                                             aaaGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAAtt                                                                                 >  1:661118/1‑141 (MQ=255)
                                                                gaagaTTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCAt                                                                              >  1:57431/1‑141 (MQ=255)
                                                                gaagaTTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTACCAATTCAt                                                                              >  2:520404/1‑141 (MQ=255)
                                                                  acgttCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCAttt                                                                            <  2:83291/138‑1 (MQ=255)
                                                                    aTTCATATCAAGTTGTATATCTGCGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCAttttc                                                                          <  2:142933/141‑1 (MQ=255)
                                                                    aTTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGCTGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATGTAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCCttttt                                                                          >  2:308792/1‑140 (MQ=255)
                                                                       cATATCAAGATGTATATTTTGGCTAAATAAATTTGGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCAttttcttt                                                                       <  1:533828/141‑1 (MQ=255)
                                                                       cATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCAttttcttt                                                                       <  1:797145/141‑1 (MQ=255)
                                                                            cAAGATGTATATCATGGTTAAATGCATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTTTCATTAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAATTTTATGCTATCAATTCATTTTCTTTTCTgg                                                                  <  2:723977/141‑1 (MQ=255)
                                                                              aGATGTATACCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTCCCATCGATTTTTTGTTAGCATCCATATAAAATTGCGAAACGACTAGTTATGTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGta                                                                <  1:371158/141‑1 (MQ=255)
                                                                                 tGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATAc                                                             <  1:707389/141‑1 (MQ=255)
                                                                                  gTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTTTTTTTTTTTATATAAAATTGCAAAACGACTAGTTTTTTTGCATTTCAAACTTTATGCTATTAATTCATTTTCTTTTCTGCTATACt                                                            >  2:140058/1‑141 (MQ=255)
                                                                                  gTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACt                                                            >  2:331227/1‑141 (MQ=255)
                                                                                  gTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACt                                                            <  2:751653/141‑1 (MQ=255)
                                                                                     tatCTTGGTTAAATAAATTTTGCTATTTAGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACtttt                                                         <  1:776948/141‑1 (MQ=255)
                                                                                     tatCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTTTTTTTTTTTTTATAAAATAGCAAAACGACTAATTTTTTTGCGTTTCAAAGTTTATGATTTCAATTCTTtctcttttctcatatgttttt                                                         >  2:442058/1‑130 (MQ=255)
                                                                                     tatCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACtttt                                                         <  1:623576/141‑1 (MQ=255)
                                                                                     tatCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACtttt                                                         <  1:156299/141‑1 (MQ=255)
                                                                                      ttCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACttttt                                                        <  2:142780/140‑1 (MQ=255)
                                                                                       tCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTta                                                       <  2:285664/141‑1 (MQ=255)
                                                                                          tGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGTTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATAc                                                    <  2:270371/141‑1 (MQ=255)
                                                                                          tGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATAc                                                    <  2:270202/141‑1 (MQ=255)
                                                                                           ttttAAATAAATTTTACGATGGGTCAACTTTACCATCGCTTTTTTGTTCTCATCCATATAAAACGACAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACt                                                   <  2:504424/139‑1 (MQ=255)
                                                                                             ttAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCACTGTAAAATACGAAGACGTCTAGTTCTTTTGCCTCTCGAACTTTCTGCTATCCATTCATTTTCTTCTCACGCATACCTCTTATCCTaa                                                 >  2:839029/1‑141 (MQ=255)
                                                                                             ttAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTaa                                                 >  2:80548/1‑141 (MQ=255)
                                                                                             ttAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTaa                                                 >  2:80536/1‑141 (MQ=255)
                                                                                             ggaaataaatTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATTCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACtga                                                 >  2:119117/3‑139 (MQ=255)
                                                                                               acataaatATTGCGATGGTGCAACTGTACAATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTAACACATAATGCGCGTTTCTGGTATACTTTTTATACTAACt                                               >  2:173509/3‑141 (MQ=255)
                                                                                                 ataaatTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATCCTAACTta                                             >  1:119274/1‑141 (MQ=255)
                                                                                                 ataaatTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTta                                             <  1:207226/141‑1 (MQ=255)
                                                                                                  taaatTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATCATAACTtat                                            >  1:708645/1‑141 (MQ=255)
                                                                                                         tGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGGCTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTCTACTTTTTATACTAACTTatagaaaca                                     >  1:230541/1‑139 (MQ=255)
                                                                                                          gCGATGGTGCAACTGTACCATCGCTTTTTTTTTTGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATTAATTCATTTTCTTTTCTGTTATACTTTTTATTCTAACCTatagaaaaaa                                    >  2:20668/1‑140 (MQ=255)
                                                                                                          gCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACGTataaaaaaat                                    >  1:815745/1‑141 (MQ=255)
                                                                                                             aTGGTGCAAGTGTACCATCGCTTTTTTTTTTTTTTTTTTTTAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGTTATACTTTTTATACTAACTTatataaaatttga                                 >  2:183940/1‑136 (MQ=255)
                                                                                                               gttGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTatagaaaaatagaaa                               <  2:479964/139‑1 (MQ=255)
                                                                                                               ggTGCAACTGTACCATCGCTTTTTTGTTTTCATCTATATAAAATTGCAACACGACTAGTTATTTTGCATTTCAAACTTTATTCTATCATTTCCTTTTCTTTTCTGGTCTACTTTTTATACTAACTGatagaaaaatagaaa                               >  2:316297/1‑141 (MQ=255)
                                                                                                               ggTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTttaaaaaactagaaa                               >  1:298469/1‑141 (MQ=255)
                                                                                                                    aaccttACCATCGCTTTTTTGTTCTCAACAAAATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATATTTTTTATTCTAACTTATAGAAAAAAAAAAAAagag                          <  2:459731/136‑1 (MQ=255)
                                                                                                                     aCTGTACCATCGCTTTTTTGTTTTTTTCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTATACaaaaatagaaaaatagc                         >  2:303724/1‑140 (MQ=255)
                                                                                                                     aCTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTATAGAAAAATAGAAAAAGAgg                         >  1:129707/1‑141 (MQ=255)
                                                                                                                       tGTACCATCGCTTTTTTTTTTTTTTTTTTATAAAATTGCAAACCGACTAGTTATTTTGCATTTCAAAGTTTATGCTATCATTTCTTTTTCTTTTTTGGTATACTTGTTATACTATCTTATATATCAATAGTAACAGAGGca                       >  2:709321/1‑140 (MQ=255)
                                                                                                                        gTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTATAGAAAAATAGAAAAAGAGGCGa                      <  1:805902/141‑1 (MQ=255)
                                                                                                                        gTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACCATATGAAATGTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTATAGAAAAATAGAAAAAGAGGCGa                      <  1:395057/141‑1 (MQ=255)
                                                                                                                            cATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTCACTTATAGAAAAATAGAAAAAGAGGCAATGCa                  >  2:71137/1‑141 (MQ=255)
                                                                                                                            cATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTCATACTAACTTATAGAAAAATAGAAAAAGAGGCGATGCa                  <  2:661118/141‑1 (MQ=255)
                                                                                                                             ctCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTATAGAAAAATAGAAAAAGAGGCGATGCAt                 <  2:483201/140‑1 (MQ=255)
                                                                                                                              tCGCTTTTTTTTTTTTTTCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTTTACTTACTTATAGAAAAATAGAAAAAGAAGCGATGCATa                >  2:731335/1‑141 (MQ=255)
                                                                                                                               cGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTATAGAAAAATAGAAAAAGAGGCGATGCATaa               <  1:577423/141‑1 (MQ=255)
                                                                                                                               cGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTATAGAAAAATAGAAAAAGAGGCGATGCATaa               <  1:673528/141‑1 (MQ=255)
                                                                                                                                gCTTTTTCGTTCGCATCCATCAAAAATTGCAAAAAGACTCGTTATTATGCATTTCAACATTTATACTATCGATTCATTTTCTTTTCTGGTATACTGTGTATACTAACTGATAGAAAAATAGAAAAAGAGGCGATGCATAAt              <  2:827210/141‑1 (MQ=255)
                                                                                                                                  ttttttGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTATAGAAAAATAGAAAAAGAGGCGATGCATAATgg            <  1:203876/141‑1 (MQ=255)
                                                                                                                                   tttttGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTATAGAAAAATAGAAAAAGAGGCGATGCATAATGGc           <  1:313227/141‑1 (MQ=255)
                                                                                                                                     tttGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTATAGAAAAATAGAAAAAGAGGCGATGCATAATGGCAt         >  1:268981/1‑141 (MQ=255)
                                                                                                                                            gCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTATAGAAAAATAGAAAAAGAGGCGATGCATAATGGCATTTCACGa  <  2:515680/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
CGCTCGAGAAAATAATTTGAAAATTATTGCCTCATGTTCATTTGCCAAACATATGTTAGAAAAAGAAGATTCATATCAAGATGTATATCTTGGTTAAATAAATTTTGCGATGGTGCAACTGTACCATCGCTTTTTTGTTCGCATCAATATAAAATTGCAAAACGACTAGTTATTTTGCATTTCAAACTTTATGCTATCAATTCATTTTCTTTTCTGGTATACTTTTTATACTAACTTATAGAAAAATAGAAAAAGAGGCGATGCATAATGGCATTTCACGA  >  CP000730/2657432‑2657712

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: