BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,161	T→C	33.2%	I131V (ATT→GTT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,161	0	T	C	33.2%	196.6 / 201.6	245	I131V (ATT→GTT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (70/93); new base C (38/43); total (109/136)
Fisher's exact test for biased strand distribution p-value = 5.86e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

Only 100 of 252 total aligned reads displayed.

TTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36022‑36301

tttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAAtt                                                                                                                                             <  2:95753/141‑1 (MQ=255)

 ttcttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCCTTGAAGGGGCCTGATATGAAAttc                                                                                                                                            >  2:167059/1‑140 (MQ=255)

  tcttATTTTAATATTGCGGTTATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTCAGTTTAAAAGCTTTGATTACTTTACCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTAc                                                                                                                                           <  2:160482/141‑1 (MQ=11)

   cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTTCTACCTTCGTTGAAGGTGCCTGATCTGGACTTAcc                                                                                                                                          >  1:6309/1‑141 (MQ=255)

   cttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATCACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGACGGTGCCTGAGTTGtacctacc                                                                                                                                          >  2:212722/1‑141 (MQ=11)

   cttACTTTAATATGACGGAGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTAcc                                                                                                                                          >  2:74675/1‑141 (MQ=255)

    ttACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATATACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTCACTTCGTTGAAGGTGCCTGATCTGTAATTACCt                                                                                                                                         >  1:250110/1‑141 (MQ=14)

     tACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCtt                                                                                                                                        <  2:278716/141‑1 (MQ=255)

     tACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCtt                                                                                                                                        <  2:301249/141‑1 (MQ=255)

       cTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTCCTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCtttt                                                                                                                                      <  1:179046/141‑1 (MQ=255)

         ttAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTTCCTTTTGa                                                                                                                                    >  2:290660/1‑141 (MQ=21)

           aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAgg                                                                                                                                  <  1:370608/141‑1 (MQ=25)

           aaTATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCACTTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAgg                                                                                                                                  >  1:69213/1‑141 (MQ=11)

                gACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAc                                                                                                                             <  2:106975/141‑1 (MQ=255)

                 aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCCGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGCTCTGTAATTACCTTTTTAGGTTTAcc                                                                                                                            >  2:45555/1‑141 (MQ=11)

                 aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTGTAACCATTGCTACCGTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                            <  1:217246/141‑1 (MQ=255)

                 aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTCAGCCTTAcc                                                                                                                            >  2:173595/1‑141 (MQ=11)

                  cGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCa                                                                                                                           <  1:337370/141‑1 (MQ=11)

                  cGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCa                                                                                                                           <  2:64606/141‑1 (MQ=11)

                   ggTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTACTTACCTTTTGAGGTTTACCaa                                                                                                                          <  2:187568/141‑1 (MQ=12)

                    gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                         <  1:149182/141‑1 (MQ=17)

                    gTGATCTTGCTCAAGGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaaa                                                                                                                         <  2:336996/141‑1 (MQ=11)

                      gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAAGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAtt                                                                                                                       >  2:154242/1‑141 (MQ=12)

                        tCTTGCTCAATGAGGTTATTCAGCTATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCGATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                     >  2:267264/1‑141 (MQ=11)

                        tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                     >  1:325456/1‑141 (MQ=17)

                        tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                     >  2:294986/1‑141 (MQ=12)

                           tGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTa                                                                                                                  <  1:186193/141‑1 (MQ=32)

                            gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTaa                                                                                                                 <  2:185073/141‑1 (MQ=17)

                              tCAATGAGGGTATTCAGTTATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAGAAGCTTTAATTAGTTTAACCATTGCTTCCTTCGTTGAAGGTGTCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATg                                                                                                               <  2:70321/141‑1 (MQ=11)

                                aaTGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACTACATAGTTTAATgag                                                                                                             >  2:66785/1‑141 (MQ=11)

                                  tGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGAc                                                                                                           <  1:105459/141‑1 (MQ=11)

                                   gAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACg                                                                                                          <  2:261188/141‑1 (MQ=17)

                                   gAGGTTATTCAGATAATTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGAACTGTAATTACCTTTTAAGGTGTACCAAATTGTTTAATGAGACg                                                                                                          <  2:79980/141‑1 (MQ=11)

                                    gggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                         <  2:234086/140‑1 (MQ=12)

                                    aGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                         <  2:301587/141‑1 (MQ=17)

                                    aGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                         >  1:318977/1‑141 (MQ=12)

                                     ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAATCATTGCTACCTTTGTTGAAGGTTCCTGATCTGTAATTACATTTTGTTGTTTGCTAAATTGTTTAATGAGACGtt                                                                                                        >  1:361793/1‑141 (MQ=11)

                                     ggTTATTCAGATATTTCGATGTACAATGCCAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGtt                                                                                                        <  1:201173/141‑1 (MQ=11)

                                      gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                       <  2:10176/141‑1 (MQ=17)

                                       ttATTCAGATATTTCGATGGACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTg                                                                                                      >  1:197112/1‑141 (MQ=12)

                                       ttATTCAAATATTTCGATTCACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                                                                                                      <  2:249908/141‑1 (MQ=11)

                                        tATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                     >  2:193166/1‑141 (MQ=12)

                                        tATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                     <  1:222428/141‑1 (MQ=12)

                                         aTTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGAt                                                                                                    >  2:206500/1‑141 (MQ=255)

                                                      gATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                                                       >  1:317441/1‑141 (MQ=255)

                                                       aTGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                      <  2:327609/141‑1 (MQ=17)

                                                       aTGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGGAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                      <  1:27075/141‑1 (MQ=255)

                                                       aTGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                      >  2:309093/1‑141 (MQ=32)

                                                       aTGTACAATGACAGCCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                      <  2:355741/141‑1 (MQ=11)

                                                           aCAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                  <  2:282106/141‑1 (MQ=17)

                                                           aCAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATg                                                                                  >  2:131837/1‑141 (MQ=255)

                                                            cAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                 >  1:199260/1‑141 (MQ=11)

                                                            cAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                 >  1:153818/1‑141 (MQ=255)

                                                            cAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                 >  1:265812/1‑141 (MQ=255)

                                                             aaggACAGTCAGGTTTAAGTTGAAAAGCTTTAATTATGTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                                <  2:118688/138‑1 (MQ=11)

                                                             aaTGGCAGTCATGTTTAAGTTTAACAGCTTTAATTACTTTAACCATTGCTAGTTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGTTTACCAAATTTTTTAATGAGACGTTTGATAAACTCATATGCTGAATGAt                                                                                <  2:131135/141‑1 (MQ=11)

                                                             aaTGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGAt                                                                                <  1:196308/141‑1 (MQ=32)

                                                                                                  tttAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                           <  1:230869/141‑1 (MQ=11)

                                                                                                  ttgaaccATGGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                           <  1:46607/136‑1 (MQ=2)

                                                                                                   ttAGCCTTTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTCCCTTTTGAGGCTTACCAAATTGTTTAATGAGCCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatgt                                          <  2:27449/141‑1 (MQ=11)

                                                                                                     aaccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                        <  2:304595/139‑1 (MQ=17)

                                                                                                     aaccAGTGCTACCTTGGTTGAAGCTGCCTTATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                        <  1:262840/139‑1 (MQ=255)

                                                                                                     aGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                        >  1:342631/1‑141 (MQ=32)

                                                                                                      gCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTccc                                       >  2:23965/1‑141 (MQ=255)

                                                                                                       ccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCct                                      >  1:309676/1‑141 (MQ=32)

                                                                                                        ctttGTTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                     <  2:9319/139‑1 (MQ=17)

                                                                                                        cATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAATCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                     <  1:315077/141‑1 (MQ=17)

                                                                                                        cATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTGGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                     <  1:32939/141‑1 (MQ=17)

                                                                                                          ttGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg                                   >  1:299310/1‑141 (MQ=32)

                                                                                                          ttGCTACCTTCGTTGAAGGTGCCTGAACTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg                                   <  1:228350/141‑1 (MQ=17)

                                                                                                           tgttgccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgt                                  <  2:374363/136‑2 (MQ=255)

                                                                                                           tgctgcCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgt                                  <  1:283439/141‑2 (MQ=12)

                                                                                                           tgctgcCTTCGTGGAAGGTGCCTGTTCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTATTGGTACGTTTGATGAACGCATATGCTGAATGATTATCTCGTTGCTTACGCATCCAAATATCTAATGTATGTCCCTCTgt                                  <  2:354009/141‑2 (MQ=11)

                                                                                                             cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTAt                                >  2:344917/1‑141 (MQ=17)

                                                                                                              tgccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                               <  2:229210/139‑1 (MQ=255)

                                                                                                                cttttgTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAAGTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                             <  1:358602/136‑1 (MQ=11)

                                                                                                                ccTTCGTTGAAGGTTCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTGATGTATGTCCCTCTGTATCaa                             >  1:203350/1‑141 (MQ=11)

                                                                                                                ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCTCCCTCACGATAATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCaa                             >  1:176971/1‑141 (MQ=11)

                                                                                                                ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                             >  1:124576/1‑141 (MQ=17)

                                                                                                                ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                             <  1:240015/141‑1 (MQ=255)

                                                                                                                ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                             <  1:214842/141‑1 (MQ=255)

                                                                                                                ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                             >  1:305335/1‑141 (MQ=12)

                                                                                                                  ttCGTTGCAGGTGCCTTATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAACCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                           <  1:305012/141‑1 (MQ=11)

                                                                                                                  ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATg                           <  1:340586/141‑1 (MQ=32)

                                                                                                                      ttGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCAc                       <  1:71747/141‑1 (MQ=32)

                                                                                                                            gTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGAtata                 <  2:197112/141‑1 (MQ=17)

                                                                                                                            gTGCCTGATCTGTAATCACTATTTGAGGTTCACCAAATTGTTAAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGACCCTCTGTATCAATGGCACGAtata                 <  1:238671/141‑1 (MQ=11)

                                                                                                                             tGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatttca                >  1:100983/1‑137 (MQ=17)

                                                                                                                               ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAAt              >  1:134410/1‑141 (MQ=32)

                                                                                                                               ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAAt              >  1:140675/1‑141 (MQ=32)

                                                                                                                                cTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATa             <  2:143411/141‑1 (MQ=32)

                                                                                                                                cTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTCATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGGAAGCGCCTCTGTATCAATGGCACGATATAAATa             <  2:247167/141‑1 (MQ=11)

                                                                                                                                cTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAGGACACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATa             <  2:323816/141‑1 (MQ=12)

                                                                                                                                   aTCTGAAATTACTTGTTGAGGTTTCCACCATTGTCTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCTCCCTGTATCAATGGCACGATATAAATAGCt          <  1:34293/141‑1 (MQ=11)

                                                                                                                                    tCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTc         <  2:26437/141‑1 (MQ=32)

                                                                                                                                     cTGTAACTACCCTTTAAGGCTTACCAAATTGCTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTcc        <  1:118383/141‑1 (MQ=11)

                                                                                                                                      tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa       <  1:220395/141‑1 (MQ=17)

                                                                                                                                      tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCa       <  1:152401/141‑1 (MQ=32)

                                                                                                                                        tAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtt     <  1:206500/141‑1 (MQ=12)

                                                                                                                                          aTTACCTTTTTAGGTTTACCAAATCGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCAtttt   <  1:294253/141‑1 (MQ=17)

TTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTC  >  CP000730/36022‑36301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`