BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,174	T→C	34.5%	K126K (AAA→AAG)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,174	0	T	C	34.5%	179.0 / 206.6	247	K126K (AAA→AAG)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base T (73/87); new base C (36/50); total (109/138)
Fisher's exact test for biased strand distribution p-value = 5.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

Only 100 of 248 total aligned reads displayed.

TATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTG  >  CP000730/36035‑36312

  tGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTa                                                                                                                                         >  1:299378/1‑141 (MQ=25)

    aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCCGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGCTCTGTAATTACCTTTTTAGGTTTAcc                                                                                                                                       >  2:45555/1‑141 (MQ=11)

    aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                                       >  1:283796/1‑141 (MQ=21)

    aCGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAcc                                                                                                                                       >  1:244402/1‑141 (MQ=25)

     cGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCa                                                                                                                                      <  1:337370/141‑1 (MQ=11)

      ggTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGTTACTTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaa                                                                                                                                     <  2:204886/141‑1 (MQ=255)

       ttgatcttggctgaTGAGGTTATTCAGATATTTAGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                                    <  2:131354/128‑1 (MQ=11)

       gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                                    >  1:219345/1‑141 (MQ=17)

       gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCaaa                                                                                                                                    <  1:149182/141‑1 (MQ=17)

       gTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaaa                                                                                                                                    <  1:131275/141‑1 (MQ=11)

       gTGATCTTGCTCAAGGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCaaa                                                                                                                                    <  2:336996/141‑1 (MQ=11)

         taTCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTGAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAtt                                                                                                                                  <  1:67471/140‑1 (MQ=255)

         gATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAAGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAAtt                                                                                                                                  >  2:154242/1‑141 (MQ=12)

           tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGt                                                                                                                                >  2:294986/1‑141 (MQ=12)

           tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCGATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                                >  2:267196/1‑141 (MQ=11)

           tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                                <  1:227327/141‑1 (MQ=11)

           tCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATGTTAGCCATTGCAGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGt                                                                                                                                >  2:279057/1‑141 (MQ=11)

            cTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGtt                                                                                                                               >  1:134885/1‑141 (MQ=17)

               gCTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTaa                                                                                                                            <  2:185073/141‑1 (MQ=17)

               gCTCAATGAGGTTATTCAGATATTTCAATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTaa                                                                                                                            <  1:179493/141‑1 (MQ=12)

                cTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                           <  2:175132/141‑1 (MQ=17)

                cTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                           <  1:321417/141‑1 (MQ=12)

                cTCAATGAGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAAt                                                                                                                           <  2:190543/141‑1 (MQ=17)

                 tCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATg                                                                                                                          <  2:76787/141‑1 (MQ=32)

                 tCAATGAGGGTATTCAGTTATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAGAAGCTTTAATTAGTTTAACCATTGCTTCCTTCGTTGAAGGTGTCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATg                                                                                                                          <  2:70321/141‑1 (MQ=11)

                   aaTGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACTACATAGTTTAATgag                                                                                                                        >  2:66785/1‑141 (MQ=11)

                     tGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGAc                                                                                                                      <  1:105459/141‑1 (MQ=11)

                       gggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                                    <  2:234086/140‑1 (MQ=12)

                       aGGTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGt                                                                                                                    <  1:230050/141‑1 (MQ=17)

                       aGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGt                                                                                                                    <  1:253416/141‑1 (MQ=11)

                       aGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGt                                                                                                                    >  1:56821/1‑141 (MQ=11)

                       aGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGt                                                                                                                    <  1:177507/141‑1 (MQ=255)

                        ggTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAATCATTGCTACCTTTGTTGAAGGTTCCTGATCTGTAATTACATTTTGTTGTTTGCTAAATTGTTTAATGAGACGtt                                                                                                                   >  1:361793/1‑141 (MQ=11)

                         gTTATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGttt                                                                                                                  <  1:195762/141‑1 (MQ=17)

                          ttATTCAAATATTTCGATTCACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTg                                                                                                                 <  2:249908/141‑1 (MQ=11)

                           tATTCAGATATTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                                >  2:193166/1‑141 (MQ=12)

                           tATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGa                                                                                                                <  1:237799/141‑1 (MQ=11)

                           tATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTAACAAATTGTTTAATGAGACGTTTGa                                                                                                                <  1:238934/141‑1 (MQ=17)

                              tCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATaa                                                                                                             >  1:306325/1‑141 (MQ=255)

                                   tatTTCGATGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCa                                                                                                        >  1:216872/1‑141 (MQ=12)

                                        cGATGTACAATGGCAGTCAGGGTTAAGTTTAAAAGCTTTAATTACTTTAACCAATCCTCCCATCGTTTAATGTGCCTGGTCTGAAATGACCTTTTGAGGTTTACCAAATTGTTTAATGAGATGTTTGATAAACGCATATGc                                                                                                   <  1:198090/141‑1 (MQ=11)

                                         gATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCt                                                                                                  >  1:317441/1‑141 (MQ=255)

                                          aTGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                                 <  2:327609/141‑1 (MQ=17)

                                          aTGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGGAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                                 <  1:27075/141‑1 (MQ=255)

                                          aTGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                                 <  2:307587/141‑1 (MQ=11)

                                          aTGTACAATGACAGCCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTg                                                                                                 <  2:355741/141‑1 (MQ=11)

                                           tGTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGa                                                                                                <  1:316454/141‑1 (MQ=17)

                                            gTACAATGGCAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGaa                                                                                               <  1:142530/141‑1 (MQ=17)

                                             tACAATGACTGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTCGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAAt                                                                                              <  2:97606/141‑1 (MQ=11)

                                               cAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                            >  1:153818/1‑141 (MQ=255)

                                               cAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTATTTTAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGa                                                                                            >  1:265812/1‑141 (MQ=255)

                                                                                     tttAGCCATTGCTGCCTTCGTTGAAGGTGGCTGTTCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGTCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                                      <  2:166263/141‑1 (MQ=11)

                                                                                     ttgaaccATGGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatg                                                      <  1:46607/136‑1 (MQ=2)

                                                                                      ttAGCCATTGCTGCCTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatgt                                                     <  1:196825/141‑1 (MQ=9)

                                                                                      ttAACCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAatgtatgt                                                     <  2:309208/141‑1 (MQ=14)

                                                                                        aGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTcc                                                   >  1:342631/1‑141 (MQ=32)

                                                                                          ccATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCct                                                 >  1:309676/1‑141 (MQ=32)

                                                                                           ctttGTTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                                <  2:9319/139‑1 (MQ=17)

                                                                                           cATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCctc                                                <  2:127567/141‑1 (MQ=32)

                                                                                             ttGCTACTTTCGTTGAAGGTGGCTGATCTGTAATTACCTTTTGAGGTTTGCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTg                                              <  2:225728/141‑1 (MQ=12)

                                                                                              tgttgccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTgt                                             <  2:374363/136‑2 (MQ=255)

                                                                                              tgctgcCTTCGTGGAAGGTGCCTGTTCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTATTGGTACGTTTGATGAACGCATATGCTGAATGATTATCTCGTTGCTTACGCATCCAAATATCTAATGTATGTCCCTCTgt                                             <  2:354009/141‑2 (MQ=11)

                                                                                                cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGGCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATGTAATGTATGTCACTCTGTAt                                           >  2:238671/1‑141 (MQ=255)

                                                                                                cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTAt                                           >  2:344917/1‑141 (MQ=17)

                                                                                                 tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATc                                          <  1:222330/141‑1 (MQ=17)

                                                                                                   cttttgTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAAGTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                        <  1:358602/136‑1 (MQ=11)

                                                                                                   ccTTCGTTGAAGGTTCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTGATGTATGTCCCTCTGTATCaa                                        >  1:203350/1‑141 (MQ=11)

                                                                                                   ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                        >  2:25143/1‑141 (MQ=17)

                                                                                                   ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTACGTCCCTCTGCATCaa                                        <  2:113540/141‑1 (MQ=17)

                                                                                                   ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                        <  1:240015/141‑1 (MQ=255)

                                                                                                   ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                        <  1:214842/141‑1 (MQ=255)

                                                                                                     ttCGTTGCAGGTGCCTTATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAACCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                                      <  1:305012/141‑1 (MQ=11)

                                                                                                     ttCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATg                                      <  1:340586/141‑1 (MQ=32)

                                                                                                      tCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATgg                                     <  2:301423/141‑1 (MQ=255)

                                                                                                        gTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCa                                   <  1:153302/141‑1 (MQ=12)

                                                                                                         ttGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCAc                                  >  1:119019/1‑141 (MQ=12)

                                                                                                              ggTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatat                             >  2:28415/1‑141 (MQ=255)

                                                                                                               gTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAGTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGAtata                            <  2:325456/141‑1 (MQ=17)

                                                                                                               gTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGAtata                            <  2:197112/141‑1 (MQ=17)

                                                                                                                tGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCGATGGCACGATATaa                           >  2:58826/1‑141 (MQ=17)

                                                                                                                tGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatttca                           >  1:100983/1‑137 (MQ=17)

                                                                                                                  ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATAACTAATGTATGTCCCTCTGCCTCACTGGCACGCTATaaaa                         >  2:136259/1‑140 (MQ=255)

                                                                                                                   cTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTCATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGGAAGCGCCTCTGTATCAATGGCACGATATAAATa                        <  2:247167/141‑1 (MQ=11)

                                                                                                                      aTCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCt                     <  2:262659/141‑1 (MQ=12)

                                                                                                                      aTCTGAAATTACTTGTTGAGGTTTCCACCATTGTCTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCTCCCTGTATCAATGGCACGATATAAATAGCt                     <  1:34293/141‑1 (MQ=11)

                                                                                                                          ttAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAt                 <  2:271817/140‑1 (MQ=17)

                                                                                                                          gTAATTACCTTTTGATGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGTTATAAATAGGTCCAt                 >  1:98174/1‑141 (MQ=255)

                                                                                                                           tAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtt                <  1:206500/141‑1 (MQ=12)

                                                                                                                            tactACCTTTTGAGTCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAttt               <  1:267264/138‑1 (MQ=255)

                                                                                                                            aaccaCCTTTGGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAttt               <  1:279057/137‑1 (MQ=255)

                                                                                                                             aTTACCTTTTTAGGTTTACCAAATCGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCAtttt              <  1:294253/141‑1 (MQ=17)

                                                                                                                             aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtttt              >  2:302972/1‑141 (MQ=17)

                                                                                                                             aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtttt              >  1:326591/1‑141 (MQ=17)

                                                                                                                              ttACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTc             >  1:199765/1‑141 (MQ=32)

                                                                                                                              ctgccTTTTGAGGCTTAACAAATTGTTTAATGATTCGTTTGATAAACGCATATGCTGAATGACTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTc             <  1:74675/138‑1 (MQ=11)

                                                                                                                               tACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTcc            <  2:276089/141‑1 (MQ=32)

                                                                                                                                 ccTTTTGAGGGTTACTAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtt          <  1:113727/141‑1 (MQ=17)

                                                                                                                                   ttttGAGGTTTACCAAATTGTTTAATGAGACGTGTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtttt        >  2:246105/1‑141 (MQ=17)

                                                                                                                                      tGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAAGGTATGTCCCTCTGTATCAATGGCACGATATAAATAGACCCATTTTCATTTTaat     >  2:213637/1‑139 (MQ=11)

                                                                                                                                       gAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTAttt    >  1:115258/1‑141 (MQ=17)

TATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGTACAATGACAGTCAGGTTTAAGTTTAAAAGCTTTAATTACTTTAGCCATTGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTG  >  CP000730/36035‑36312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`