BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,269	C→T	61.1%	A95T (GCA→ACA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,269	0	C	T	61.1%	91.8 / 286.8	280	A95T (GCA→ACA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (62/46); new base T (84/86); total (147/133)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.65e-01

Only 100 of 284 total aligned reads displayed.

TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36129‑36409

tGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGc                                                                                                                                              >  1:157139/1‑141 (MQ=32)

tGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGGTTGGTAAACGCATATGCTGAGTGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGc                                                                                                                                              >  2:93491/1‑141 (MQ=12)

  cTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTAt                                                                                                                                            >  2:344917/1‑141 (MQ=17)

   tACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATc                                                                                                                                           >  2:368216/1‑141 (MQ=32)

     cttttgTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAAGTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                                                                                                                         <  1:358602/136‑1 (MQ=11)

     ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCTCCCTCACGATAATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCaa                                                                                                                                         >  1:176971/1‑141 (MQ=11)

     ccTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                                                                                                                         >  1:124576/1‑141 (MQ=17)

     ccTTCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCaa                                                                                                                                         <  1:214842/141‑1 (MQ=255)

      cTTCGTTGAAGGTGCCTGATTTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                                                                                                                                        <  1:150042/141‑1 (MQ=255)

      cTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCAGGTGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAAt                                                                                                                                        >  1:271833/1‑141 (MQ=12)

       ttCGTTGCAGGTGCCTTATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAACCGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATg                                                                                                                                       <  1:305012/141‑1 (MQ=11)

        tCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATgg                                                                                                                                      <  2:218958/141‑1 (MQ=32)

        tCGTTGAAGGTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATgg                                                                                                                                      <  2:301423/141‑1 (MQ=255)

          gTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGcc                                                                                                                                    >  1:153596/1‑140 (MQ=17)

              aaGGTGCCTTATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGat                                                                                                                                <  2:209234/141‑1 (MQ=17)

                ggTGCCTGATCTGTAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatat                                                                                                                              >  1:239467/1‑141 (MQ=12)

                  tGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGatttca                                                                                                                            >  1:100983/1‑137 (MQ=17)

                    ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAAt                                                                                                                          >  1:44724/1‑141 (MQ=17)

                    ccTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATAACTAATGTATGTCCCTCTGCCTCACTGGCACGCTATaaaa                                                                                                                          >  2:136259/1‑140 (MQ=255)

                     cTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTCATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGGAAGCGCCTCTGTATCAATGGCACGATATAAATa                                                                                                                         <  2:247167/141‑1 (MQ=11)

                        aTCTGAAATTACTTGTTGAGGTTTCCACCATTGTCTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCTCCCTGTATCAATGGCACGATATAAATAGCt                                                                                                                      <  1:34293/141‑1 (MQ=11)

                         tCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTc                                                                                                                     <  2:26437/141‑1 (MQ=32)

                          cTGTAACTACCCTTTAAGGCTTACCAAATTGCTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTcc                                                                                                                    <  1:118383/141‑1 (MQ=11)

                           tGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCa                                                                                                                   <  1:220395/141‑1 (MQ=17)

                            gTAATTACCTTTTGATGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGTTATAAATAGGTCCAt                                                                                                                  >  1:98174/1‑141 (MQ=255)

                             tAACTACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtt                                                                                                                 <  1:206500/141‑1 (MQ=12)

                              aactACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAttt                                                                                                                <  1:267196/138‑1 (MQ=12)

                               actACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtttt                                                                                                               >  2:327324/3‑141 (MQ=12)

                               aTTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCAtttt                                                                                                               >  1:326591/1‑141 (MQ=17)

                                 tACCTTTTGAGGCTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAAGGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCGCCATTTTcc                                                                                                             <  2:239467/141‑1 (MQ=255)

                                   ccTTTTGAGGTTTACCAAATTGTTTAATTAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtt                                                                                                           <  1:240599/141‑1 (MQ=17)

                                   ccTTTTGAGGGTTACTAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtt                                                                                                           <  1:113727/141‑1 (MQ=17)

                                    cTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCttt                                                                                                          >  1:135193/1‑141 (MQ=17)

                                     ttttGAGGTTTACCAAATTGTTTAATGAGACGTGTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCtttt                                                                                                         >  2:246105/1‑141 (MQ=17)

                                        tGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCGCCATTTTCCTTTTAtt                                                                                                      >  2:303221/1‑141 (MQ=17)

                                          aGGTTTACCAAATGGTTTAATGAGACGTTTGATGAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTCCCTCAATGGCACTATATACATAGCTCCATTTTCCTTTGAtttt                                                                                                    >  2:195311/1‑141 (MQ=11)

                                              ttACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtt                                                                                                >  2:249234/1‑140 (MQ=17)

                                               tACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtat                                                                                               <  2:322545/141‑3 (MQ=17)

                                               tACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTAGGTCACTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGt                                                                                               >  2:339836/1‑141 (MQ=17)

                                                aCCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGAtata                                                                                              <  2:272692/141‑4 (MQ=17)

                                                    aaTTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGtc                                                                                          <  1:234956/141‑1 (MQ=32)

                                                        ttttAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCGTTTATTTTGATATACGTCtcat                                                                                      <  2:23312/140‑1 (MQ=255)

                                                        gTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCtcat                                                                                      <  1:332415/141‑1 (MQ=32)

                                                           tAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTTTTTTGATGTGCGTCTCATCaa                                                                                   >  1:139883/1‑141 (MQ=12)

                                                             aTGAGACGTTTGATAAACGCATATGCTGAATGATTCTCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTGTACGTCTCATCAAtc                                                                                 >  2:281408/1‑140 (MQ=12)

                                                             aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCATTTATATTTGATATACGTCTCATCAATa                                                                                 <  1:180528/141‑1 (MQ=11)

                                                             aTGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATCGCTCCATTTTCCTTTTATTTTGATGTACGTATCATCAATa                                                                                 >  2:154379/1‑141 (MQ=17)

                                                              tGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATAc                                                                                <  1:19791/141‑1 (MQ=32)

                                                                ataCGTTGAATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATAGCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGc                                                                              <  2:69213/139‑1 (MQ=11)

                                                                agcggttgtaTAAACACATATTCTCAATAATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGc                                                                              <  1:84212/132‑1 (MQ=11)

                                                                agaCGTTTGATAAACGCATATGCTGACTGATTATGTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAACGGCACTATATAAATAGCTCCATTTTCCTTTTATTTTGGTGTACGTCTCATCAATACGc                                                                              <  1:226303/141‑1 (MQ=11)

                                                                  aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCCACCAAATATCAAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCGCCATTTTCCTTTTATTTTGATGTACGTCTCCTCAACACGcaa                                                                            >  2:37336/1‑139 (MQ=11)

                                                                  aCGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCa                                                                            <  1:25143/141‑1 (MQ=17)

                                                                       tGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATTGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTTATCAATACTCCTTCTGt                                                                       >  1:66066/1‑141 (MQ=11)

                                                                       tGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGCTATACGCTTCATCAATACGCCCTTTga                                                                       >  1:260914/1‑140 (MQ=11)

                                                                        taTAAACGCATTTGCTGAATGAGGATCGCTTTGCTTACGCAACCAAATATCTATTGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGta                                                                      <  2:59418/140‑1 (MQ=11)

                                                                         aTAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCACTGTGAATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGtaa                                                                     <  1:368216/141‑1 (MQ=12)

                                                                            aaCGCATATGTTGAATTATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGtaataa                                                                  <  2:317441/141‑1 (MQ=255)

                                                                                cATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTTCATCAATGTCTCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTATGACTCATGAATCCGACATTTATAATATGCtt                                                              >  1:27694/1‑141 (MQ=11)

                                                                                  tatGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtttt                                                            <  2:340904/141‑1 (MQ=25)

                                                                                    tGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTACTAAGCtttttt                                                          >  2:52506/1‑141 (MQ=255)

                                                                                    tGCTGAATGATGAGCTCGTTGCTTACGCAACCAAATATCTAATGTATGGCCCACGGTATCAATGGCACGATATAAATAGCTCCATTTTTCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCtttttt                                                          <  2:145559/141‑1 (MQ=11)

                                                                                     tcTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTa                                                         <  1:78013/140‑1 (MQ=25)

                                                                                           tGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCttt                                                   >  2:297539/1‑141 (MQ=255)

                                                                                                 tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTTTCATCATTACGCCATTGTTAGTACGCTTTTTTAGTCTTTttctgc                                             >  1:308993/1‑139 (MQ=14)

                                                                                                 tctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                             >  1:55233/1‑141 (MQ=40)

                                                                                                 tctcGTTGCTTACGCAACCAAATATCCAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTttcttc                                             <  2:335553/141‑1 (MQ=255)

                                                                                                  ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATTGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                            <  2:44724/141‑1 (MQ=255)

                                                                                                  ctcGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                            <  2:199260/141‑1 (MQ=255)

                                                                                                  cccGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTcc                                            <  1:106798/139‑1 (MQ=255)

                                                                                                     gTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAATGCTTTTTATGCTTTTTCTTCCcaa                                         >  2:105943/1‑141 (MQ=11)

                                                                                                       tGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGAACGATATAGATACCTCCATTTTCCTTTTAATTAGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAAtt                                       <  2:311952/141‑1 (MQ=14)

                                                                                                            aCGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCAATTTGTAATAAGCGCTTTTATGCTTTTTCTTCCAAATTTGATa                                  >  1:287705/1‑141 (MQ=255)

                                                                                                            aCGCAACCAAATATCTAATGTATGGCCCTCTGCATCAATGGCACGATATAAATATCGCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAGGCTTTTTTATGCTTTTTCTTCCAAATTTgttt                                  >  2:21601/1‑138 (MQ=14)

                                                                                                              gCAGCCAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                <  2:26908/141‑3 (MQ=255)

                                                                                                              gCACCCAAATATCTAATGTATGTACCTCTGTATAATTGGCACAATATAAATAGCACCCTATTCCTTTTATTTTGCTATACCTCGACTCCATATACTAATCGTTTGAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                >  2:113394/1‑139 (MQ=2)

                                                                                                              gCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATata                                <  1:303567/141‑3 (MQ=38)

                                                                                                               cAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATataa                               <  1:97893/141‑4 (MQ=38)

                                                                                                                 aCCAAATATCTAATGTATGTCCCTAAAAATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaaa                             >  2:171190/1‑141 (MQ=11)

                                                                                                                  ccAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCAATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAt                            >  1:345130/1‑141 (MQ=255)

                                                                                                                       tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATATATACCGCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCTAAATTTGATATAAAATTgggg                       <  1:193166/141‑1 (MQ=11)

                                                                                                                       tatCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgggg                       <  2:83808/141‑1 (MQ=255)

                                                                                                                         tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATCGGGGCa                     >  2:130731/1‑141 (MQ=11)

                                                                                                                          cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                    <  1:336716/141‑1 (MQ=255)

                                                                                                                             atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                 >  2:118947/1‑141 (MQ=38)

                                                                                                                             atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAACTTTGTAAAAAATTGGGGCATAtt                 >  1:247239/1‑141 (MQ=255)

                                                                                                                             atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTATCATGAATAGGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAAAAAATTTGGTGATAtt                 >  2:132722/1‑141 (MQ=14)

                                                                                                                               gtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTTTCATCAATACTCAATTTGTAATAAGCTGTTTTATTCTTTTTCTTCCAAATTTGATAGAAAATTGAGGCATATTCt               >  1:283246/1‑141 (MQ=11)

                                                                                                                                 atgtCCCTCTGCATCAATGGCACGATATCAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTGTGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg             <  1:210529/141‑1 (MQ=255)

                                                                                                                                  tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa            >  2:260038/1‑141 (MQ=25)

                                                                                                                                  tgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGa            <  1:294753/141‑1 (MQ=25)

                                                                                                                                  tgtCCCTCTGCATCAATGACACGATATAAATAGCCCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATCTGATACCTAATTGCGGCATATTCTTGa            >  2:29368/1‑141 (MQ=14)

                                                                                                                                    tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATAGAAAATTGGGGCATATTCTTGacc          >  1:40976/1‑139 (MQ=255)

                                                                                                                                    tCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGAGATAATATTTTGGTAGATGCTTGAAc          >  1:200650/1‑141 (MQ=11)

                                                                                                                                    tCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATCTTCTTGAAc          >  2:191585/1‑141 (MQ=255)

                                                                                                                                        tctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTATTTAACCCaa      >  2:314037/1‑141 (MQ=255)

                                                                                                                                        tctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCCTCCAAATTTGATATAAAATTGGGGCATATTGTTGAAtccgt      >  2:356484/1‑139 (MQ=255)

                                                                                                                                           gCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACgg   <  1:131510/141‑1 (MQ=40)

                                                                                                                                           gCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTCTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTCATATAAAGTTGGGGCTTCTTATTGAGCCCAActg   >  1:279851/1‑139 (MQ=11)

                                                                                                                                            cATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGt  <  2:87294/141‑1 (MQ=18)

TGCTACCTTCGTTGAAGGTGCCTGATCTGTAATTACCTTTTGAGGTTTACCAAATTGTTTAATGAGACGTTTGATAAACGCATATGCTGAATGATTATCTCGTTGCTTACGCAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGT  >  CP000730/36129‑36409

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`