BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,378	C→T	80.9%	L58L (TTG→TTA)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,378	0	C	T	80.9%	368.2 / 98.1	255	L58L (TTG→TTA)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (27/21); new base T (119/83); total (151/104)
Fisher's exact test for biased strand distribution p-value = 7.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 256 total aligned reads displayed.

CAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATC  >  CP000730/36240‑36518

  aCCAAATATCTAATGTATGTCCCTAAAAATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATaaaa                                                                                                                                          >  2:171190/1‑141 (MQ=11)

    cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGTTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAtt                                                                                                                                        >  1:277615/1‑141 (MQ=255)

    cAAATATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAAtt                                                                                                                                        >  2:281863/1‑141 (MQ=255)

      aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTgg                                                                                                                                      <  1:294986/141‑1 (MQ=255)

      aaTATCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTACCAAATTTGATATAAAATTgg                                                                                                                                      >  1:201462/1‑141 (MQ=255)

         atCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGc                                                                                                                                   <  1:344917/141‑1 (MQ=255)

         atCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATAAGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATTgggga                                                                                                                                   >  2:245417/1‑140 (MQ=11)

          tCTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGATATAAAATCGGGGCa                                                                                                                                  >  2:130731/1‑141 (MQ=11)

          tCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCa                                                                                                                                  >  2:131510/1‑141 (MQ=255)

           cTAATGTATGTCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCat                                                                                                                                 <  1:336716/141‑1 (MQ=255)

            tAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTATTATAAAATTGGGTCata                                                                                                                                >  2:30302/1‑141 (MQ=255)

              atgtatgtCCCTCTGTATCAATGGCACGCTATAAATAGCTCCATTTTCCTTTTATATTGATATACGTCTCATCAATACGCCAATTGTAATAAGCTTCTTTATGCTTTTTCTTCCAAATTTGTTATAAAATTGTGGAatcct                                                                                                                              >  2:235197/1‑138 (MQ=11)

              atgtatgtCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATAtt                                                                                                                              >  2:118947/1‑141 (MQ=38)

                gtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTTTCATCAATACTCAATTTGTAATAAGCTGTTTTATTCTTTTTCTTCCAAATTTGATAGAAAATTGAGGCATATTCt                                                                                                                            >  1:283246/1‑141 (MQ=11)

                gtatgtCCCTCTGTATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGGTATAAAATTGGGGCATATTCt                                                                                                                            >  1:286583/1‑141 (MQ=255)

                  atgtCCCTCTGCATCAATGGCACGATATCAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTGTGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTg                                                                                                                          <  1:210529/141‑1 (MQ=255)

                   tgtCCCTCTGCATCAATGACACGATATAAATAGCCCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATCTGATACCTAATTGCGGCATATTCTTGa                                                                                                                         >  2:29368/1‑141 (MQ=14)

                     tCCCTCTGCATCGATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTTCGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTCTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAc                                                                                                                       >  1:263784/1‑141 (MQ=255)

                         tctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGCTATAAAATTGGGGCATATTCTTGAACCCaa                                                                                                                   >  2:341770/1‑141 (MQ=21)

                         tctGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTATTTAACCCaa                                                                                                                   >  2:314037/1‑141 (MQ=255)

                           tatatgtaTGGCACGATATACATAGCACCATTTTCCTTTTATTGTTATAGACGTCTCATCAATACGCCATTTGTAATAAGCGTTTTTATGCTTTTTCGTCCAAATTTGATATACACTTGGGTCATTTTCTTGAACCCAACg                                                                                                                 <  2:361793/134‑1 (MQ=11)

                            gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                                >  2:195149/3‑141 (MQ=255)

                            gtaTCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACgg                                                                                                                >  1:261069/3‑141 (MQ=255)

                             catctgtgtcACGATATAAATTGCTCCATTTTCATTTTGTTTGGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAATATTGGGGCATATTCTTGAACCCAACGGt                                                                                                               <  2:257675/132‑1 (MQ=14)

                               tCAATGGCACGATATAAATAGATCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAg                                                                                                             >  2:106950/1‑141 (MQ=21)

                                cAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCAGATTCTTGATCCCAACGGTAGa                                                                                                            <  1:193517/141‑1 (MQ=255)

                                  aTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGagt                                                                                                          >  2:142606/1‑139 (MQ=255)

                                   tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGatcg                                                                                                         <  1:94636/141‑4 (MQ=255)

                                   tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCg                                                                                                         <  1:13127/141‑1 (MQ=255)

                                    ggCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTCATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCCACGGTCGACCGt                                                                                                        >  2:356164/1‑141 (MQ=255)

                                       aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                     >  1:61633/1‑141 (MQ=21)

                                       aCGATATAAATAGCTCCATTTTCCTATTATTCTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                     <  1:101474/141‑1 (MQ=11)

                                        cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCTTGTTCTTGAACCGAAGGGTAGatgattgaa                                                                                                    >  1:13013/1‑133 (MQ=11)

                                        cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                    >  1:372491/1‑141 (MQ=255)

                                        cGATATAAATAGCTCCATCATGGCGATATGTTTATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTCAACCCAACGGTAGACCGTTGaa                                                                                                    >  1:89602/1‑141 (MQ=11)

                                             taAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCCCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAatgatg                                                                                               <  1:12297/141‑1 (MQ=21)

                                              aaaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGGAGACCGTTGAAtgatga                                                                                              >  2:331334/1‑141 (MQ=255)

                                              aaaTAGCTCCATTTTCCCTTTCTTTTGATATCCGTCTCATCAATACACCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGAAATAAAATTGGGCCCAATTCTTGACCCCAACGTTAGACCGCTCAatgatca                                                                                              >  2:76587/1‑139 (MQ=11)

                                                aTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAc                                                                                            <  2:177785/141‑1 (MQ=255)

                                                   gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                         >  1:189418/1‑141 (MQ=255)

                                                       cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTTATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTgca                                                                                     >  2:84621/1‑141 (MQ=255)

                                                       cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                     >  1:83311/1‑141 (MQ=255)

                                                        aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaccc                                                                                    >  2:51543/1‑139 (MQ=255)

                                                         ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                   <  1:313741/141‑1 (MQ=255)

                                                         ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                   <  2:306325/141‑1 (MQ=255)

                                                           ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                                 <  1:38257/141‑1 (MQ=255)

                                                           ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacaac                                                                                 >  2:11281/1‑141 (MQ=255)

                                                           tgccTTGTGTTTTCATATACCTCTCATCCATACGCCATTTGTCAGAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAACTTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAGGATGCACGTTTAcaccac                                                                                 <  2:123526/139‑1 (MQ=11)

                                                            tgattttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACACAACGGTAGATAGTTGAATGATGAACGTTTacacaaag                                                                                >  2:371443/4‑137 (MQ=11)

                                                            tCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTGATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAGCCACAGGTAGATCGTTGCATGATGCACGTTATCACCACg                                                                                >  1:115135/1‑141 (MQ=11)

                                                             ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAACATTGGGGCATATTCTTGAACCCACCTGTAGACCGTTTGATGATGAACGTTTAGACCACGt                                                                               >  1:504/1‑141 (MQ=11)

                                                             ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTCAACCGTTGTATTATTAACCTTTagtccaagt                                                                               >  2:210541/1‑133 (MQ=11)

                                                             ccTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAAACCACGt                                                                               >  1:27019/1‑141 (MQ=255)

                                                             ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATCTTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                               >  2:59433/1‑141 (MQ=255)

                                                              cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                              >  1:246389/1‑141 (MQ=255)

                                                              cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAAGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTAAACCCAACGGGAGATCGTTAAAGGATAAACGTTTacacgccagt                                                                              >  2:322694/1‑135 (MQ=11)

                                                               ttttATTTTGATATACGTCTCATCAATACGCCATTTGTGATAAGCTGTTTTATGCTTTTTCTTCCAAATTGGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTc                                                                             >  1:219283/1‑141 (MQ=11)

                                                                 ttATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTccc                                                                           >  2:23371/1‑141 (MQ=25)

                                                                 ttATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCCCGTTctc                                                                           >  1:171932/1‑139 (MQ=11)

                                                                 ttACTTTGAAATACGTCTCATAAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctc                                                                           <  1:66785/141‑3 (MQ=11)

                                                                  tATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct                                                                          <  2:271833/141‑4 (MQ=255)

                                                                     tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGACCCCAACGGTAGACCGTTGAATGATGAACGTTTACACAACGTTCCCTTaa                                                                       <  2:217888/141‑1 (MQ=255)

                                                                     tttGATATACGTCTCATCAATACGTCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTaa                                                                       <  1:173595/141‑1 (MQ=11)

                                                                        gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTGCTCTTAatat                                                                    >  2:69032/5‑141 (MQ=11)

                                                                        gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                    <  1:106950/141‑1 (MQ=25)

                                                                        gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                    >  1:235591/1‑141 (MQ=21)

                                                                           atACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTc                                                                 <  1:140847/140‑1 (MQ=255)

                                                                            tACGTCTCATCAATACGCCATTTGTAATGAGCTTTTTTATGCTTTTTCTTCCAAATTGGATATAAAATTGGGGGATATTCTTGAACTCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGCTCCCTTAATAtttaa                                                                >  2:50631/1‑139 (MQ=11)

                                                                            tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                >  2:336915/1‑141 (MQ=21)

                                                                            tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACGCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                <  1:248395/141‑1 (MQ=255)

                                                                            tACGGCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                <  1:199001/141‑1 (MQ=255)

                                                                                tcatatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAACCCAAGGTAGATCGTTGAATGATGAAAGTTTACACCACGTTCTCTTAATATTTCAGata                                                            <  1:39281/137‑1 (MQ=11)

                                                                                   catcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGatatat                                                         >  1:77929/1‑141 (MQ=40)

                                                                                    atcaATACGCCATTTGTAATAAGCTTTTTTTTTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGTTCCCTTAATGTTTCAGATCTATc                                                        >  2:120829/1‑141 (MQ=11)

                                                                                    atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                        <  1:302972/141‑1 (MQ=255)

                                                                                    atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                        >  2:223690/1‑141 (MQ=25)

                                                                                    atcaATACGCCATTTCTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATc                                                        >  1:276651/1‑141 (MQ=255)

                                                                                     tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATcc                                                       >  1:2129/1‑140 (MQ=25)

                                                                                     tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                       <  2:63236/141‑1 (MQ=40)

                                                                                      caATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCAc                                                      >  1:239567/1‑141 (MQ=25)

                                                                                       aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACg                                                     <  1:346515/141‑1 (MQ=255)

                                                                                         ttcGCCATTTGGAATAAGCTTTTTTATGCTTTTTCTTCCAGATTCGATATAAAATGGGGGCATATTCTTGAACCCAACGGTAGATCGGTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                   <  1:329425/139‑1 (MQ=11)

                                                                                          acgcgcttggTAATAAGCTTGTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTCGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                  <  2:149561/132‑1 (MQ=11)

                                                                                          aCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATa                                                  <  2:153596/141‑1 (MQ=255)

                                                                                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTTTTTTTTTTTTTATATAAAATTGGGGCATATTCTTGAACCCAATGGTAGATCGTTGAATGATGGACGTTTCCAACACTTTCTCTTAATATTTCTGTTATATCACGAttt                                                 >  2:327366/1‑139 (MQ=11)

                                                                                           cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                 >  2:263878/1‑141 (MQ=21)

                                                                                            gCCATTTGTAATAAGCTTTTTTACTCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAc                                                <  1:132722/141‑1 (MQ=255)

                                                                                             ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                               >  1:305753/1‑141 (MQ=21)

                                                                                             ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGCTGATCGTCTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAcc                                               >  1:160389/1‑140 (MQ=255)

                                                                                             ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                               >  2:269315/1‑141 (MQ=40)

                                                                                               aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTGGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCa                                             <  2:298374/141‑1 (MQ=18)

                                                                                                tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                            >  1:363028/1‑141 (MQ=11)

                                                                                                  tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATg                                          >  1:370347/1‑141 (MQ=40)

                                                                                                    taataaGCTTTTTTATGCTTTTTGCATCGAATTTTATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                        >  1:89319/1‑139 (MQ=11)

                                                                                                    taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGta                                        <  2:199253/141‑1 (MQ=40)

                                                                                                            ttttttATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACCGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAg                                >  2:208074/1‑141 (MQ=37)

                                                                                                                   tGCTTTTTCTTCCAATTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagtag                         <  2:342631/141‑1 (MQ=25)

                                                                                                                         ttcttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCGTAGATAGTAGCCAACg                   <  2:127356/141‑1 (MQ=25)

                                                                                                                             tcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTa               >  2:252134/1‑141 (MQ=35)

                                                                                                                                       aTACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAAc     >  2:295590/1‑141 (MQ=35)

CAACCAAATATCTAATGTATGTCCCTCTGCATCAATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATC  >  CP000730/36240‑36518

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`