BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	36,413	C→T	49.5%	V47I (GTC→ATC)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	36,413	0	C	T	49.5%	18.9 / 233.0	186	V47I (GTC→ATC)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (59/35); new base T (44/48); total (103/83)
Fisher's exact test for biased strand distribution p-value = 5.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.10e-01

Only 100 of 187 total aligned reads displayed.

AATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCA  >  CP000730/36273‑36550

aaTGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCCTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAATTTTGATCTAAAATTGGGGCATATTCTTGAACCCAACGGTAGAc                                                                                                                                           >  1:213949/1‑141 (MQ=255)

  tGGCACGATATACATAGAGCCATTTTCCTGTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCg                                                                                                                                         <  2:250110/141‑1 (MQ=11)

  tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCg                                                                                                                                         <  1:318072/141‑1 (MQ=40)

  tGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCg                                                                                                                                         <  1:13127/141‑1 (MQ=255)

      aCGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGa                                                                                                                                     >  1:61633/1‑141 (MQ=21)

      aCGATATAAATAGCTCCATTTTCCTATTATTCTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGa                                                                                                                                     <  1:101474/141‑1 (MQ=11)

      aCGATATAAATAGCTCCATTCTCCTTTTATTTTGCTGTACGTCTGATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATACTCTTGAACCCTACGGTAGACCGTTGa                                                                                                                                     >  1:226587/1‑141 (MQ=14)

       cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTTaa                                                                                                                                    >  2:209491/1‑141 (MQ=11)

       cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                                                    >  1:372491/1‑141 (MQ=255)

       cGATATAAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGaa                                                                                                                                    <  2:146819/141‑1 (MQ=255)

       cGATATAAATAGCTCCATTTCCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCATCGGTAGACCGTTGaa                                                                                                                                    <  1:276132/141‑1 (MQ=255)

            taAATAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAatgatg                                                                                                                               >  2:273290/1‑141 (MQ=255)

             aaaTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAtgatga                                                                                                                              >  1:266406/1‑141 (MQ=255)

             aaaTAGCTCCATTTTCCCTTTCTTTTGATATCCGTCTCATCAATACACCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAACTTTGAAATAAAATTGGGCCCAATTCTTGACCCCAACGTTAGACCGCTCAatgatca                                                                                                                              >  2:76587/1‑139 (MQ=11)

              aaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTTCGAAACTGATATCAAATTGGGGCATATTCTTGAACCCAACGGTCGCCCGTTGAatgatgta                                                                                                                             >  1:177083/1‑139 (MQ=11)

              aaTAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGaa                                                                                                                             >  1:98391/1‑141 (MQ=25)

              aaTAGCTCCATTTTCCTTTTATTTTGATCTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGaa                                                                                                                             <  1:23965/141‑1 (MQ=255)

               aTAGCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAc                                                                                                                            <  2:177785/141‑1 (MQ=255)

                  gCTCCATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGtt                                                                                                                         >  1:189418/1‑141 (MQ=255)

                      cATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTTATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTgca                                                                                                                     >  2:84621/1‑141 (MQ=255)

                      cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTaca                                                                                                                     >  1:83311/1‑141 (MQ=255)

                      cATTTTCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAAGTTTaca                                                                                                                     >  2:325511/1‑141 (MQ=255)

                       tttttTCTTTTATTTTGATGTACGTCTCAGCAATTCGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGGTATAAAATTGGGGTATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                                                    <  1:195311/140‑1 (MQ=11)

                       aTTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTacac                                                                                                                    <  2:219345/141‑1 (MQ=18)

                        ttttCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTAcacc                                                                                                                   >  1:67842/1‑141 (MQ=40)

                        ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTACTACGAATGTGGAGGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                                                   <  1:103640/141‑1 (MQ=11)

                        ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                                                   <  2:306325/141‑1 (MQ=255)

                        ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                                                   <  1:313741/141‑1 (MQ=255)

                        ttttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacc                                                                                                                   <  2:292805/141‑1 (MQ=255)

                         tttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAAAGTTTAcacca                                                                                                                  >  2:115596/1‑141 (MQ=255)

                          ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                                                                 <  1:38257/141‑1 (MQ=255)

                          ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcaccac                                                                                                                 >  1:82877/1‑141 (MQ=255)

                          ttCCTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTAcacaac                                                                                                                 >  2:11281/1‑141 (MQ=255)

                          tgccTTGTGTTTTCATATACCTCTCATCCATACGCCATTTGTCAGAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAACTTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAAGGATGCACGTTTAcaccac                                                                                                                 <  2:123526/139‑1 (MQ=11)

                           tCCTTTTATTTGGATATACGTCTTATAAGTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACg                                                                                                                <  2:151392/141‑1 (MQ=11)

                            ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATCTTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                                                               >  2:59433/1‑141 (MQ=255)

                            ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCACCGGTAGATCGTTGAATGATGAACGTTTACACAACGt                                                                                                               >  2:298021/1‑141 (MQ=11)

                            ccTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGt                                                                                                               <  2:243211/141‑1 (MQ=255)

                             cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                                              >  1:246389/1‑141 (MQ=255)

                             cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGtt                                                                                                              >  2:373021/1‑141 (MQ=255)

                             cTTTTATTTTGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTAAGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTAAACCCAACGGGAGATCGTTAAAGGATAAACGTTTacacgccagt                                                                                                              >  2:322694/1‑135 (MQ=11)

                             cTTTTATTTAGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTCTCTTCCAATTCTGATACAAAATTTGGGCATATTCTTGAACCCAACGGTACATCGTTGAATGATGAACATTTACACCCCgct                                                                                                              >  2:132947/1‑139 (MQ=14)

                              ttttATTTTGATATACGTCTCATCAATACGCCATTTGTGATAAGCTGTTTTATGCTTTTTCTTCCAAATTGGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTc                                                                                                             >  1:219283/1‑141 (MQ=11)

                                ttACTTTGAAATACGTCTCATAAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctc                                                                                                           <  1:66785/141‑3 (MQ=11)

                                 tATTTTGATATACGTCTCCTCAATACGCCATTTGTAATAAGCTTTTTTAGGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTctct                                                                                                          <  1:38186/141‑1 (MQ=11)

                                    tttGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGCACCCAACGGTAGACCGTTGAATGATGAACGTTTACACAACGTTCCCTTaa                                                                                                       >  2:255387/1‑141 (MQ=255)

                                     ttGATATACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAat                                                                                                      <  1:297539/141‑1 (MQ=11)

                                       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACGCTAGGTTCTCTTAatat                                                                                                    >  2:295522/5‑141 (MQ=11)

                                       gatatACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTGCTCTTAatat                                                                                                    >  2:69032/5‑141 (MQ=11)

                                       gATGTACGTCTCATCAATACGCTACATGAAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                                    >  1:89601/1‑141 (MQ=11)

                                       gATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAatat                                                                                                    <  1:106950/141‑1 (MQ=25)

                                          gTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACCGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTc                                                                                                 >  2:34467/1‑141 (MQ=255)

                                          atACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTc                                                                                                 <  1:140847/140‑1 (MQ=255)

                                           tACGTCTCATCAATACGCCATTTGTAATGAGCTTTTTTATGCTTTTTCTTCCAAATTGGATATAAAATTGGGGGATATTCTTGAACTCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGCTCCCTTAATAtttaa                                                                                                >  2:50631/1‑139 (MQ=11)

                                           tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAGCGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCa                                                                                                <  1:273290/141‑1 (MQ=255)

                                           tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                >  2:336915/1‑141 (MQ=21)

                                           tACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACTGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCa                                                                                                <  2:269726/141‑1 (MQ=255)

                                             cGTCTCATCAATACGCCATTTGTAATAAGCTTTCTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTCCTCTTAATATTTCAGa                                                                                              >  1:53139/1‑141 (MQ=11)

                                               tcatatcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAAACCCAAGGTAGATCGTTGAATGATGAAAGTTTACACCACGTTCTCTTAATATTTCAGata                                                                                            <  1:39281/137‑1 (MQ=11)

                                                  catcaATACGCCAGTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGatatat                                                                                         <  1:173091/141‑1 (MQ=11)

                                                   atcaATACGCCATTTGTAATAAGCTTTTTTTTTCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCCCGTTCCCTTAATGTTTCAGATCTATc                                                                                        >  2:120829/1‑141 (MQ=11)

                                                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                                                        >  1:127887/1‑141 (MQ=21)

                                                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATc                                                                                        >  2:223690/1‑141 (MQ=25)

                                                   atcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTGTACACCACGTTCCCTTAATATTTCAGATATATc                                                                                        >  2:207323/1‑141 (MQ=25)

                                                    tcaATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCa                                                                                       <  2:63236/141‑1 (MQ=40)

                                                    tcaATACGCCAGTTGTAATAAGCTTTTTTATGCTCTTTCTTCCAAAGTTGATATAAAATTGGGGCATATTCTTGAACCCACCGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCa                                                                                       <  2:53165/141‑1 (MQ=11)

                                                      aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACg                                                                                     <  1:346515/141‑1 (MQ=255)

                                                      aaTACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACg                                                                                     <  2:213549/141‑1 (MQ=40)

                                                       aTACGCCATTTTTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACACAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGa                                                                                    <  2:234889/141‑1 (MQ=255)

                                                        ttcGCCATTTGGAATAAGCTTTTTTATGCTTTTTCTTCCAGATTCGATATAAAATGGGGGCATATTCTTGAACCCAACGGTAGATCGGTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGAt                                                                                   <  1:329425/139‑1 (MQ=11)

                                                        tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                                                   <  1:262239/141‑1 (MQ=18)

                                                        tACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGAt                                                                                   >  1:303206/1‑141 (MQ=21)

                                                         aCGCCATTTGTAATAAGCTTTTTTATGCATTTCCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATa                                                                                  <  1:92412/141‑1 (MQ=255)

                                                          cGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATaa                                                                                 >  2:263878/1‑141 (MQ=21)

                                                          cGCCATTTGTAATAAGCTTTTTTATGCTCTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACCGTCGATCGTTCAATGATGAACGTTTAGCCCCCGTTCTCCTCATATTTCAGATATATCACCAtcc                                                                                 >  2:177277/1‑139 (MQ=11)

                                                           gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                                <  1:281863/141‑3 (MQ=255)

                                                           gCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATagc                                                                                >  1:143331/1‑139 (MQ=255)

                                                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                               >  1:305753/1‑141 (MQ=21)

                                                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                               >  1:235741/1‑141 (MQ=21)

                                                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                               >  1:110358/1‑141 (MQ=21)

                                                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGCTGATCGTCTACACCACGTTCCCTTAATATTTCAGATATATCACGATAAcc                                                                               >  1:160389/1‑140 (MQ=255)

                                                            ccATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACt                                                                               >  2:269315/1‑141 (MQ=40)

                                                              aTTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACCTTCCCTTAATATTTCAGATATATCACGATAACTCa                                                                             >  1:122461/1‑141 (MQ=25)

                                                               tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                            <  2:329041/141‑1 (MQ=11)

                                                               tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCaa                                                                            >  1:363028/1‑141 (MQ=11)

                                                               tttGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGCTAACTCaa                                                                            >  2:121894/1‑141 (MQ=21)

                                                                 tGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTGCAAAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATg                                                                          <  1:367602/141‑1 (MQ=11)

                                                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATATAAAATTGGGGCATATTCTTGAACCCAACGGTAGATCGTTGAATGATGAACGTTTACACCACGTTCTCTTAATATTTCAGATATATCACGATAGCTCAATgca                                                                        >  1:211522/1‑139 (MQ=11)

                                                                   taataaGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGta                                                                        <  2:199253/141‑1 (MQ=40)

                                                                                  tGCTTTTTCTTCCAATTTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGAtagtag                                                         <  2:342631/141‑1 (MQ=25)

                                                                                    ggtgttgttcCAAAGTTGATACAAAAGTGGGGCATATTCCTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCGTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGcc                                                       <  2:160389/134‑1 (MQ=11)

                                                                                       tttCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAAc                                                    <  2:309676/141‑1 (MQ=35)

                                                                                        ttcttcCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCGTAGATAGTAGCCAACg                                                   <  2:127356/141‑1 (MQ=25)

                                                                                                       tACAGAATTGGGGCATATTCTTGACCCAAAAGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATAAAACGCTAACTCAATCTATATCTTACATAGTAGCCACCGGATACAGTATTAAct                                    >  2:227361/1‑140 (MQ=11)

                                                                                                       tACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGCTTACACCCCGTTCCCTTAATATTTCAGATATATCACGCTAACTCAATGGATATCTTAGATAGTAGCCACCGGCTACAGTGATAACa                                    >  2:369909/1‑141 (MQ=11)

                                                                                                         cAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATc                                  <  1:333134/141‑1 (MQ=33)

                                                                                                                ggggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTa                           <  2:20437/141‑1 (MQ=32)

                                                                                                                  ggCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTaaa                         >  1:288914/1‑141 (MQ=32)

                                                                                                                                  cAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGaaa         >  2:332419/1‑141 (MQ=32)

                                                                                                                                     cGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAg      >  1:43728/1‑141 (MQ=32)

AATGGCACGATATAAATAGCTCCATTTTCCTTTTATTTTGATGTACGTCTCATCAATACGCCATTTGTAATAAGCTTTTTTATGCTTTTTCTTCCAAATTTGATACAAAATTGGGGCATATTCTTGAACCCAACGGTAGACCGTTGAATGATGAACGTTTACACCACGTTCCCTTAATATTTCAGATATATCACGATAACTCAATGTATATCTTAGATAGTAGCCAACGGCTACAGTGATAACATCCTTGTTAAATTGTTTATATCTGAAATAGTTCA  >  CP000730/36273‑36550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`