Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 76,295 T→C 52.7% intergenic (+353/‑781) USA300HOU_0072 → / → USA300HOU_0075 universal stress protein/lysophospholipase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP00073076,2950TC52.7% 19.3 / 275.1 240intergenic (+353/‑781)USA300HOU_0072/USA300HOU_0075universal stress protein/lysophospholipase
Reads supporting (aligned to +/- strand):  ref base T (65/47);  new base C (73/51);  total (142/98)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 242 total aligned reads displayed.

TAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTAC  >  CP000730/76157‑76426
                                                                                                                                          |                                                                                                                                   
gaGGTTCTGTTGCAAAGTTGAATTTATAGTATAATTTTAACAAAAAGGCGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGttt                                                                                                                                   >  2:345130/2‑140 (MQ=2)
          tGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGatata                                                                                                                         >  2:300244/1‑141 (MQ=35)
            cAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATc                                                                                                                       >  1:128590/1‑141 (MQ=35)
               aGTTGAATGTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGACGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGa                                                                                                                    <  1:51454/141‑1 (MQ=12)
                agtGAATCTATAGTAAAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTGGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGaa                                                                                                                   <  1:290331/139‑1 (MQ=255)
                     atttataCTCTAACTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATAtt                                                                                                              >  2:266406/4‑141 (MQ=1)
                      tttataGTAAGATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGCGTTATCGTGATATATCTGAAatataa                                                                                                             >  1:273653/3‑139 (MQ=11)
                        tataatataaTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAg                                                                                                           <  1:25807/141‑1 (MQ=12)
                          taatataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAgag                                                                                                         >  2:255967/4‑139 (MQ=12)
                           aGTATAATTTTAACAAAAAGGAGTTTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGa                                                                                                        >  1:237521/1‑141 (MQ=17)
                             tataATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGCTTTAAACAATTTAACAAGGATGTTCAACCGGGAGCCGTTGCCCTCAATTTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAAc                                                                                                      <  1:87723/141‑1 (MQ=11)
                               aaaTTTTAACAAAAAGGAGTCTTCTGTATGGAGTATTTAAGAGATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgt                                                                                                    <  1:68903/140‑1 (MQ=11)
                                 aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtgg                                                                                                  >  1:130687/1‑141 (MQ=12)
                                 aTTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACgtgg                                                                                                  <  1:117299/141‑1 (MQ=12)
                                  ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGAGATGCATTGAGTTATCGTGATATATCTGCAAGATTAAGGGAACgtggt                                                                                                 >  2:82767/1‑141 (MQ=12)
                                  ttttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGAACAAAAAAACTAAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACgtggt                                                                                                 <  1:121091/141‑1 (MQ=11)
                                   tttAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACggggtg                                                                                                >  2:110358/1‑141 (MQ=17)
                                    ttAACAAAAAGGAGTCTTCTGTATTAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGCTATGCATTGAGATATCGTGATATATCTGAAATATTAAGGGAACATGgtgt                                                                                               >  2:332790/1‑141 (MQ=255)
                                    tcaaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACCGTAGCTGTTGGCTACCATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGgtgt                                                                                               <  1:191513/139‑1 (MQ=255)
                                      aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGAGATGCATTGAGTTATCGTGCTATATCTGAACTATTAAGGGAACGTGGTGTaa                                                                                             >  1:21601/1‑141 (MQ=255)
                                      aaCAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTaa                                                                                             >  1:59433/1‑141 (MQ=12)
                                        cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAc                                                                                           <  2:67836/141‑1 (MQ=17)
                                        cAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAc                                                                                           >  2:28773/1‑141 (MQ=17)
                                         aaaaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACg                                                                                          <  2:132959/141‑1 (MQ=17)
                                           aaaGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGtt                                                                                        >  1:23371/1‑141 (MQ=17)
                                              ggAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcat                                                                                     >  1:342284/1‑141 (MQ=17)
                                               gAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGTTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATAACTGAAATATTAAGAGGACGTGGTGTAGACGTtcatc                                                                                    >  2:188712/1‑141 (MQ=11)
                                                aGTCTTCTGTATGAACTATTTCAGATATCAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTtcatca                                                                                   <  1:230424/141‑1 (MQ=255)
                                                aGTCTTCTGTATGAACTATTTCAGATATAATCAGTTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatca                                                                                   >  2:343205/1‑141 (MQ=12)
                                                aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatca                                                                                   >  2:55233/1‑141 (MQ=17)
                                                aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAACTATTAAGGGAACGTGGTGTAAACGTtcatca                                                                                   >  1:75275/1‑141 (MQ=17)
                                                aGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTtcatca                                                                                   >  1:370462/1‑141 (MQ=32)
                                                 gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGGCTGTGCGAGAGAACTGAAATATTAAGGGAACGTGGTGTAAACGTTcatcat                                                                                  >  1:84621/1‑141 (MQ=11)
                                                 gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTcatcat                                                                                  >  1:171190/1‑141 (MQ=12)
                                                 gTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGCTGTAAACGTTcatcat                                                                                  >  2:268382/1‑141 (MQ=12)
                                                  tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCAtt                                                                                 <  2:47172/141‑1 (MQ=17)
                                                  tcttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTAATCAtt                                                                                 <  2:90476/141‑1 (MQ=17)
                                                   cttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTc                                                                                <  1:109016/141‑1 (MQ=17)
                                                    ttctGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAGACGTTCATCATTCa                                                                               >  2:364287/1‑141 (MQ=12)
                                                        gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTAGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAATGGAACGTGGTGTAAACGTTCATCATTCAACgg                                                                           >  1:264607/1‑141 (MQ=12)
                                                        gTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACgg                                                                           <  2:22444/141‑1 (MQ=32)
                                                         tATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACgat                                                                          <  2:35675/141‑3 (MQ=12)
                                                           tgcaCTATTTCAGATATAAACAAGTTAACAAGGATGTTAGCACTTTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCt                                                                        <  2:12903/138‑1 (MQ=11)
                                                           tGAACTATTTCAGATCTAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTACGATATGCATTGAGGTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAAAGTTCATCATTCAACGGTCt                                                                        >  2:213949/1‑141 (MQ=11)
                                                           tGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCt                                                                        >  2:5009/1‑141 (MQ=12)
                                                              aCTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTAGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTGCAACATTCAACGGTCTAcc                                                                     >  1:196248/1‑141 (MQ=255)
                                                                tATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGt                                                                   <  2:272632/141‑1 (MQ=32)
                                                                  tttCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTGAACGTTCATCATTACACGATCTACCGCTg                                                                 >  2:41242/1‑141 (MQ=11)
                                                                    tCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTggg                                                               <  1:291983/141‑1 (MQ=12)
                                                                     cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGt                                                              >  2:240464/1‑141 (MQ=17)
                                                                     cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATGATTCAAGGATCTACCGTTGGGt                                                              >  2:197192/1‑141 (MQ=11)
                                                                     cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGt                                                              >  1:62543/1‑141 (MQ=12)
                                                                     cAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACAATCTACCGTTGGGt                                                              >  2:139883/1‑141 (MQ=255)
                                                                      aGATCTAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTCCATCATTCAACGATCTACCGTTGGGtt                                                             <  1:194941/141‑1 (MQ=11)
                                                                      aGATATAAACAATTTACCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGtt                                                             >  2:171932/1‑141 (MQ=11)
                                                                      aGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGtt                                                             >  1:164112/1‑141 (MQ=32)
                                                                       gATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAACTATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTc                                                            >  2:165617/1‑141 (MQ=17)
                                                                         tataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATGGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGCCTACCGTTGGGTTCag                                                          >  1:356164/1‑140 (MQ=12)
                                                                          ataAACAATTTAACAAGGATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAg                                                         <  1:138836/141‑1 (MQ=17)
                                                                           taAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATACGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGa                                                        >  2:27763/1‑141 (MQ=17)
                                                                              aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAata                                                     >  2:261069/1‑141 (MQ=12)
                                                                              aCAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAata                                                     <  1:150092/141‑1 (MQ=255)
                                                                                 aTTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTAAACGATCTCCCGTTGGGTTCAAGAACATGc                                                  >  2:84667/1‑141 (MQ=11)
                                                                                   ttAACAAGGATGTTATCACTGTGACTGTGGGCTACGATCACAGAAACGTGCACTCTGATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGccc                                                <  1:365844/141‑1 (MQ=11)
                                                                                    tAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGcccc                                               <  1:130882/141‑1 (MQ=12)
                                                                                      aCAAGGGTGTTCTCACTGTAGCGGTTGGCTACTATCTAAGTTATGCATGGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                             >  2:370948/1‑141 (MQ=11)
                                                                                      aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTGTCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                             >  1:249234/1‑141 (MQ=255)
                                                                                      aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATGTTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                             >  1:198167/1‑141 (MQ=255)
                                                                                      aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCaa                                             >  1:98996/1‑141 (MQ=255)
                                                                                      aCAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATAATTCAACGATCTACCGTTGGGTTCAAGAATATACCCCaa                                             >  2:135193/1‑141 (MQ=11)
                                                                                       cAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGTGCTATCATAATATAACTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAt                                            >  1:113394/1‑141 (MQ=11)
                                                                                        aaGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAAtt                                           >  2:283246/1‑141 (MQ=12)
                                                                                          ggATGTTATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAAtttt                                         >  2:199765/1‑141 (MQ=17)
                                                                                            aTGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtat                                       <  1:334333/141‑1 (MQ=255)
                                                                                             tGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTtata                                      <  2:217926/141‑1 (MQ=255)
                                                                                               ttATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATc                                    >  1:28415/1‑141 (MQ=255)
                                                                                                tATCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                   >  2:235591/1‑141 (MQ=25)
                                                                                                tATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCTTGATATATTTGAAATATTAAGAGATCGTGGTGTAAACGTTCATCATTCAACGTTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCa                                   <  2:19584/141‑1 (MQ=11)
                                                                                                 aTCACTGTAGCTGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGGTCAAGAATATGCCCCAATTTTATATCaa                                  >  1:94327/1‑141 (MQ=21)
                                                                                                  tCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCaaa                                 >  2:203350/1‑141 (MQ=255)
                                                                                                      tGTAGCCGTTGGTTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTg                             <  1:303295/141‑1 (MQ=21)
                                                                                                       gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                            <  2:104872/141‑1 (MQ=35)
                                                                                                       gTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTgg                            >  2:115989/1‑141 (MQ=35)
                                                                                                        tagccaacggCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                           >  2:98174/9‑141 (MQ=11)
                                                                                                        tAGCCGTTGGCTACTATCTAAGATCTGCATTGAGCTATCGTGATATATCGGAAGTATTAAGAGACGGTGGAGAAAAGGTTCATCATTCAACGATCGAGCGTTGGGTGCAAGAATATGCCCCAAGTTTAGATCAAATTTGga                           >  2:320178/1‑141 (MQ=11)
                                                                                                        tAGCCGTTGGCTACTAGCTAAGATATGCATTGAGCTATTGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATACAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGga                           >  1:207752/1‑141 (MQ=11)
                                                                                                         aGCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCCAATTTGgaa                          >  1:29865/1‑141 (MQ=255)
                                                                                                          gctgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                         >  2:335437/4‑141 (MQ=25)
                                                                                                          gCCGTTGGCTACTATCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGgaag                         >  1:245417/1‑141 (MQ=255)
                                                                                                            tgTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGGAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAGTTTGgaagaa                       >  2:308894/2‑141 (MQ=21)
                                                                                                                 gCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATGTGCTGGAATTTTATAACAACATTGGAAGAAAAAGc                  >  2:184108/1‑141 (MQ=11)
                                                                                                                     ctaTCTAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaa              <  1:104061/141‑1 (MQ=255)
                                                                                                                      aaTCTAAGATATTCATTGAGCTAACGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaa             <  2:104296/140‑1 (MQ=11)
                                                                                                                         atAAGATATGCATTGAGCTATCGTGATATATCTGAAATATTAGGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaa          <  2:286866/140‑2 (MQ=255)
                                                                                                                          tAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaaa         >  2:207254/1‑139 (MQ=18)
                                                                                                                           aaGATATGCATGGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaacc        >  2:100983/1‑138 (MQ=11)
                                                                                                                            aGATATGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagct       >  1:316034/1‑137 (MQ=11)
                                                                                                                              atatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTGGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAACAAGCATaaaaaagctta     >  1:327324/1‑135 (MQ=11)
                                                                                                                               tatGCATTGAGCTATCGTGATATATCTGAAATATTAAGAGAACGTTGTGTAAACGTTCATCATTCAACGATCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATaaaaaagcttat    <  1:205034/141‑8 (MQ=11)
                                                                                                                                 ggCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTAc  <  1:210103/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                   
TAGGTTCTGTTGCAAAGTTGAATCTATAGTATAATTTTAACAAAAAGGAGTCTTCTGTATGAACTATTTCAGATATAAACAATTTAACAAGGATGTTATCACTGTAGCCGTTGGCTACTATCTAAGATATGCATTGAGTTATCGTGATATATCTGAAATATTAAGGGAACGTGGTGTAAACGTTCATCATTCAACGGTCTACCGTTGGGTTCAAGAATATGCCCCAATTTTATATCAAATTTGGAAGAAAAAGCATAAAAAGCTAATTAC  >  CP000730/76157‑76426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: