Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 478,502 C→A 100% D342E (GAC→GAA USA300HOU_0457 → NADH dehydrogenase (ubiquinone)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP000730478,5020CA100.0% 117.0 / NA 39D342E (GAC→GAAUSA300HOU_0457NADH dehydrogenase (ubiquinone)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/18);  total (21/18)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

TGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGG  >  CP000730/478371‑478634
                                                                                                                                   |                                                                                                                                      
tgtgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGc                                                                                                                               >  1:299566/1‑141 (MQ=255)
tgtgCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGc                                                                                                                               >  1:129975/1‑141 (MQ=255)
   gCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGCAGCTTATGGCTgg                                                                                                                            >  2:267975/1‑141 (MQ=255)
      ttGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGAtt                                                                                                                         >  1:124200/1‑141 (MQ=255)
          gtgtATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCa                                                                                                                    <  1:373249/141‑1 (MQ=255)
                       agagaatatttaggtAATATTGCAAGTTTTTTTGAGGGCAAGATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTAtt                                                                                                       <  1:175244/127‑1 (MQ=255)
                                   tttAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGt                                                                                           >  1:24104/1‑141 (MQ=255)
                                           tGCACGGTATTTTTAATGCAACGTTTTTTTTACAATCAGGTTTTATCGTGAAGTGATTCAATATTCCATAACAAGCATATGCTATTCAAGCTGATGGGTGGTTTGTCATGGGGTGTGTGTTAGCTATTATCGTGGCATTTc                                                                                   <  2:60201/141‑1 (MQ=255)
                                           tGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCCGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTc                                                                                   <  1:230681/141‑1 (MQ=255)
                                                  tATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTAAGGCTGGATTGTCATGGGACGTGTATTAGCAATTATCGTGGCATTTCTAATTTg                                                                            >  1:283343/1‑141 (MQ=255)
                                                     ttttAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGAt                                                                         >  1:276355/1‑141 (MQ=255)
                                                      tttAAAGCAACATTATTTTTACATTCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGATGGATTGTCATGGGACGTGTCTTAGCTATTAGCGTGGCCTTTGTAGTTTgtgtg                                                                        >  2:65535/1‑137 (MQ=255)
                                                       ttAAAGCAACATTATTTTTATGTTCATTTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGGTGa                                                                       >  2:361366/1‑141 (MQ=255)
                                                        tAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCGTTTCTATTTTGGATGag                                                                      >  2:98729/1‑141 (MQ=255)
                                                        tAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATggg                                                                      >  2:175746/1‑139 (MQ=255)
                                                              aaCATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTAATTGCTATTATCGTGGCTTTTCTATTTTGGATGAGTAGTGa                                                               >  2:57027/1‑141 (MQ=255)
                                                                aattattTGTTCCATCAGGTTCTACCGTGAAGCGATTCAATATTGCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACa                                                             <  1:138266/140‑1 (MQ=255)
                                                                 attattTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAg                                                            <  1:60636/141‑1 (MQ=255)
                                                                    attTTTGCATTCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTACTATTTTGGATGAGTAGTGACAGAAg                                                         >  1:274058/1‑141 (MQ=255)
                                                                          aCAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCata                                                   <  1:363246/141‑1 (MQ=255)
                                                                          aCAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTCCTAAAGAAGCTTATGGCTGGATGGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCata                                                   <  1:368937/141‑1 (MQ=255)
                                                                          aCAATACGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCata                                                   <  1:322253/141‑1 (MQ=255)
                                                                                 ggTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAgtg                                            <  1:23584/141‑1 (MQ=255)
                                                                                   ttCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGtt                                          >  2:188218/1‑141 (MQ=255)
                                                                                     cTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTaa                                        <  2:338557/141‑1 (MQ=255)
                                                                                          gTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATAGCTGGATTGTCATGGGACGTGTCTTAGCTATTATCGTGGCCTTACTATTTTGGATGAGGAGTGACATCAGTGCATATGCAGTGTTAGGTgaa                                   >  2:200388/1‑139 (MQ=255)
                                                                                           tGAAGCGATTCAATATTCCAAAACAAGCACCTGCCAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCAc                                  <  1:189993/141‑1 (MQ=255)
                                                                                                  cttCAATATGCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCt                           <  2:92286/140‑1 (MQ=255)
                                                                                                    tCAATATTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCAATccttatcg                         >  2:182893/1‑134 (MQ=255)
                                                                                                        tatTCCAAAACAAGCGTCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGAGATAAGTGCACTCATTCTAGGATg                     >  1:186706/1‑141 (MQ=255)
                                                                                                        tatTCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATg                     >  2:120202/1‑141 (MQ=255)
                                                                                                           tCCAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTc                  <  2:276355/141‑1 (MQ=255)
                                                                                                            ccAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCa                 <  2:186262/141‑1 (MQ=255)
                                                                                                             cAAAACAAGCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGCCAGGAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCAt                <  1:163522/141‑1 (MQ=255)
                                                                                                               aaaCAAGCATCTGCTAAAGAAGCTTATGGCTGGCTTGATTATTGTCGTGTATTGGCGATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTa              >  1:178801/1‑141 (MQ=255)
                                                                                                                     gCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGCGCTAGGACGGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTa        >  1:271119/1‑141 (MQ=255)
                                                                                                                     gCATCTGCTAAAGAAGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAACATACACTTAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTa        >  1:271155/1‑141 (MQ=255)
                                                                                                                       aTCTGCTAAAGAAGCCTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATc      <  1:124218/141‑1 (MQ=255)
                                                                                                                           gcgaaaGAAGCTTATGGCTGGAGTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTgg  <  1:65535/138‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
TGTGCATTGGGTGTATATTCAGCAGCGATTATTCATTTAATATTGCACGGTATTTTTAAAGCAACATTATTTTTACAATCAGGTTCTATCGTGAAGCGATTCAATATTCCAAAACAAGCATCTGCTAAAGACGCTTATGGCTGGATTGTCATGGGACGTGTATTAGCTATTATCGTGGCATTTCTATTTTGGATGAGTAGTGACAGAAGTGCATATGAAGTGTTAAGTGCACTCATTCTAGGATGGTCATTACTTGTATCTTGG  >  CP000730/478371‑478634

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: