Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,105,994 T→C 100% T12T (ACT→ACC pdhD → dihydrolipoyl dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,105,9940TC100.0% 71.3 / NA 24T12T (ACT→ACCpdhDdihydrolipoyl dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/9);  total (15/9)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGC  >  CP000730/1105863‑1106128
                                                                                                                                   |                                                                                                                                      
cacaGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATc                                                                                                                               >  1:216345/1‑141 (MQ=255)
          ttGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTc                                                                                                                     >  1:172842/1‑141 (MQ=255)
              tGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCtgg                                                                                                                 >  1:255453/1‑141 (MQ=255)
              atctGCAACTGGCCAACATTCCATGAATCACATTAAACGTTTATTCACTAAACCAGAACTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGTAGCAGGTCCtgg                                                                                                                 <  2:64493/138‑1 (MQ=255)
                         gCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATCCGTTg                                                                                                      >  1:137553/1‑141 (MQ=255)
                                   aaTGAATCACATTAAACGTTTATTAAATCATTCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCg                                                                                            >  1:370859/1‑141 (MQ=255)
                                    aTGAATCGCATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATGCGTTGCAGCAATTCgg                                                                                           >  2:16292/1‑140 (MQ=255)
                                            caTTAAACGTTTATTAAATAATCCAGAATTATTATTAATGTAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCa                                                                                   >  1:78515/1‑141 (MQ=255)
                                                               aatCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAGTTCAGGCTAAGAGTAACa                                                                <  2:172842/141‑1 (MQ=255)
                                                                  ccAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAACAGGTCCTGGTGGATACGTTGCAGAAATTCATGCAGCACAATTAGGAAAAAAAGTAaaaaag                                                             >  2:168841/1‑139 (MQ=255)
                                                                   cAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAACAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCg                                                            <  1:325795/141‑1 (MQ=255)
                                                                       attattatgAATGGAAGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGCCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTga                                                        <  1:16292/141‑1 (MQ=255)
                                                                          attattaATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATGCGTGCAGCTCAATTAGGACAAAGAGTAACAATCGTTGAGaa                                                     >  1:34577/1‑141 (MQ=255)
                                                                            attgggtGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAg                                                   <  1:214045/135‑1 (MQ=255)
                                                                                      ggggTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTtgg                                         >  1:338989/1‑141 (MQ=255)
                                                                                             aaCATGGTAGTTAGAGATTTCCCAATGGAAACAGATACCAGAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAAGTCGTGGAGCTCAATTAAGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGtt                                  <  2:143326/141‑1 (MQ=255)
                                                                                                ctGGTAGTGGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGTTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTGTGc                               <  1:268038/140‑1 (MQ=255)
                                                                                                   gTAGTTGGAGATTTCCCAATTGAAACAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTa                            >  1:314504/1‑141 (MQ=255)
                                                                                                     aggtGGAGATTTCTCAATTGAAACAGATTGCATAGTGATCGTAGGAGGTTTTGGTATATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTaaa                          <  2:339421/138‑1 (MQ=255)
                                                                                                         ggAGATTTCCCAATTGAAACCGATCCCATAATAATCGGAACAGGTCCTGGTGGATACGTTGCAGCAATTCGAACAGCTCATTTCTGACAAAAAGTAACAATCGATGAGAAAGGTAATCTTGCAGCTGTTTGCTTAAACGTa                      >  2:64391/1‑141 (MQ=255)
                                                                                                                      ttGAAACAGATACCATAGTAATCGGACCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTCAAACGTAGGATGTATTCCtt         <  1:226205/141‑1 (MQ=255)
                                                                                                                           aCAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCaaaa    >  2:82416/1‑141 (MQ=255)
                                                                                                                            cAGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAg   >  2:188047/1‑141 (MQ=255)
                                                                                                                             aGATACCATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGc  >  1:135660/1‑141 (MQ=255)
                                                                                                                                   |                                                                                                                                      
CACAGACAAATTGATGGTGCAACTGGCCAAAATGCAATGAATCACATTAAACGTTTATTAAATAATCCAGAATTATTATTAATGGAGGGGTAAAACATGGTAGTTGGAGATTTCCCAATTGAAACAGATACTATAGTAATCGGAGCAGGTCCTGGTGGATACGTTGCAGCAATTCGTGCAGCTCAATTAGGACAAAAAGTAACAATCGTTGAGAAAGGTAATCTTGGTGGTGTTTGCTTAAACGTAGGATGTATTCCTTCAAAAGC  >  CP000730/1105863‑1106128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: