Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,539,250 C→G 100% intergenic (‑349/+37) aroC ← / ← USA300HOU_1405 chorismate synthase/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,539,2500CG100.0% 68.7 / NA 22intergenic (‑349/+37)aroC/USA300HOU_1405chorismate synthase/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (9/13);  total (9/13)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

ATATAATCATTAATTAAAAATGATTAGCCGTTTTAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTCCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGTATAAAAATAATTTTT  >  CP000730/1539122‑1539383
                                                                                                                                |                                                                                                                                     
atatAATCATTAATTAAAAATGATTAGCCGTTTTAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTaaaaaaa                                                                                                                           <  2:100999/141‑1 (MQ=255)
            attaaAAATGATTAGCCGTTTTAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTaa                                                                                                               <  1:143975/141‑1 (MQ=255)
             ttaaAAATGATTAGCCGTTTTAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTaaa                                                                                                              <  2:138002/141‑1 (MQ=255)
                                    tataATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCtttt                                                                                       >  1:338176/1‑141 (MQ=255)
                                         tCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTtat                                                                                  >  1:40187/1‑141 (MQ=255)
                                           gTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATAc                                                                                >  1:346206/1‑141 (MQ=255)
                                           gTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATAc                                                                                <  1:219691/141‑1 (MQ=255)
                                              tCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACttt                                                                             >  1:284267/1‑141 (MQ=255)
                                               cGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTg                                                                            >  1:122082/1‑141 (MQ=255)
                                                 cAACATTAGCAAATAATATACTGACTACATTTCTTAATAAAAAAGAACGAACTATTAATTAATAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATACTAAAATATACATATGTTAAGCGCTTTTGTtataaaaaaaa                                                                          <  2:77704/141‑8 (MQ=255)
                                                     tttAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCa                                                                      <  2:318126/141‑1 (MQ=255)
                                                             ataataTACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAAtata                                                              <  2:43898/141‑1 (MQ=255)
                                                              taataTACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATaa                                                             <  1:236424/141‑1 (MQ=255)
                                                                                 cTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTCTTACTAATAc                                          >  2:130171/1‑141 (MQ=255)
                                                                                               acgaacTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATaaa                            >  1:107667/1‑141 (MQ=255)
                                                                                                 gaacTGTTAATTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATTAATTCTTGTACTAATACATAAAAAATATAAATg                          <  1:147944/141‑1 (MQ=255)
                                                                                                         aaTTATTAATAAAGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAg                  >  1:15067/1‑141 (MQ=255)
                                                                                                             attaATAAAGTTAATCAGTGCGTTTAAAAAAATTATGCTCTTGAAATATAGATATGTTAAGCGCTTTTGTTATACTTTGACTTCAACAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGGGAGTCAGtata              >  1:72628/1‑141 (MQ=255)
                                                                                                                  taaAGTTAATAAGGGCGTGTAAAAAAATTAGGATATTAAAATATACATATGTTAAGCGCTTTTGTTAGACTTTGACCTCAAAAATATAATCAATTCTTTTACTAATTCATAAATAATATAAATGAGAGTCAGTATAAAaat         <  2:162395/141‑1 (MQ=255)
                                                                                                                   aaaGTTAATCAATGCATAAAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGTATAAAaata        <  2:338176/141‑1 (MQ=255)
                                                                                                                     aGTTAATCAGTGCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGTATAAAaataat      <  1:96688/141‑1 (MQ=255)
                                                                                                                         aaTCAGTGCGTTTAAAAAAATTATTATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGTATAAAAATAAttttt  <  2:73752/141‑1 (MQ=255)
                                                                                                                                |                                                                                                                                     
ATATAATCATTAATTAAAAATGATTAGCCGTTTTAATATAATCGTATCGCAACTTTAGCAAATAATATACTGACTACATTTCTTAATACAAAAGAACGAACTGTTAATTATTAATAAAGTTAATCAGTCCGTTTAAAAAAATTATGATATTAAAATATACATATGTTAAGCGCTTTTGTTATACTTTGACTTCAAAAATATAATCAATTCTTTTACTAATACATAAAAAATATAAATGAGAGTCAGTATAAAAATAATTTTT  >  CP000730/1539122‑1539383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: