Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 1,647,664 A→T 12.9% Y267* (TAT→TAA)  USA300HOU_1534 ← lipoate‑‑protein ligase A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007301,647,6640AT12.9% 78.4 / 4.7 31Y267* (TAT→TAA) USA300HOU_1534lipoate‑‑protein ligase A
Reads supporting (aligned to +/- strand):  ref base A (15/12);  new base T (2/2);  total (17/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.26e-01

ATCGCAAAGCAATGCGTAAGTATAAAAGAAATCAAAGAAGAAAATAGATAAAAAAACGGAAGCACTTGTAGGTAAAATAGTCTACGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTAAAACCTTTCTCGAAAGCTTCTTCCATTTGACTAATAGTAATGTGTTCATCTG  >  CP000730/1647524‑1647800
                                                                                                                                            |                                                                                                                                        
atCGCAAAGCAATGCGTAAGTATAAAAGAAATCAAAGAAGAAAATAGATAAAAAAACGGAAGCACTTGTAGGTAAAATAGTCTACGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGa                                                                                                                                          <  1:38291/141‑1 (MQ=255)
          aaTGCGTAAGTATAAAAGAAATCAAAGAAGAAAATAGATAAAAAAACGGAAGCACTTGTAGGTAAAATAGTCTACGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAACCTTCCATTCATCTGAACGATATTTTTCAg                                                                                                                                <  2:171037/141‑1 (MQ=255)
          aaTGCGTAAGTATAAAAGAAATCAAAGAAGAAAATAGATAAAAAAACGGAAGCACTTGTAGGTAAAATAGTCTACGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCCTCTGACCGATATTTTTCAg                                                                                                                                >  2:29005/1‑141 (MQ=255)
                  aGTATAAAAGAAATCAAAGAAGCAACAACTTGTAAAAACGGAAGCACTTGTAGGTAAAATAGTCTACGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAAttct                                                                                                                        >  1:274332/1‑141 (MQ=255)
                  aGTATAAAAGAAATCAAAGAAGAAAATAGATAAAAAAACGGAAGCACTTGTAGGTAAAATAGTCTACGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAAttct                                                                                                                        >  2:340802/1‑141 (MQ=255)
                    tataAAAGAAATCAAAGAAGAAAATAGATAAAAAAACGGAAGCACTTGTAGGTAAAATAGTCTACGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAAttcttc                                                                                                                      <  1:114546/141‑1 (MQ=255)
                     ataaaagaaataaaagaaGAAAATAGATAAAAAAACGGAAGCACTTGTAGGTAAAATAGTCTACGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTcc                                                                                                                     <  2:337785/141‑1 (MQ=255)
                         aaGAAATCAAAGAAGAAAATAGATAAAAAAACGGAAGCACTTGTAGGTAAAATAGTCTACGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACtt                                                                                                                 <  1:222057/141‑1 (MQ=255)
                                        aaaaTAGATAAAAAAACGGAAGCACTTGTAGGTAAAATAGTCTACGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCtt                                                                                                  >  1:283166/1‑141 (MQ=255)
                                        aaaaTAGATAAAAAAACGGAAGCACTTGTAGGTAAAATAGTCTACGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCtt                                                                                                  >  1:81027/1‑141 (MQ=255)
                                         aaaTAGATAAAAAAACGGAAGCACTTGTAGGTAAAATAGTCTACGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTc                                                                                                 <  1:53608/141‑1 (MQ=255)
                                           aTAGATAAAAAAACGGAAGCACTTGTAGGTAAAATAGTCTACGTGCTTCCCTTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTg                                                                                               <  1:176422/141‑1 (MQ=255)
                                                                 ttGTAGGTAAAATAGTCTACGTTCTTCCATTTTTTATTCTAAAAACTACTTTCTAAGCACCTAGTTAGCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTc                                                                         <  1:86053/141‑1 (MQ=255)
                                                                               gTCTACGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCtt                                                           >  2:131259/1‑141 (MQ=255)
                                                                                tCTACGTGCTTCCATTTTTTTTTTTTTTTTCTACTTTATAAACGCCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCTCTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGTCTTACTTTCTTTTTTTTTACCttt                                                          >  2:136355/1‑141 (MQ=255)
                                                                                   aCGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCtt                                                       >  2:261371/1‑141 (MQ=255)
                                                                                      tGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTaaa                                                    >  1:47716/1‑141 (MQ=255)
                                                                                      tGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTaaa                                                    >  1:347255/1‑141 (MQ=255)
                                                                                             aTTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGACCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTCAACCTTTCTTCAAACCtttt                                             >  2:85114/1‑140 (MQ=255)
                                                                                             aTTTTTTATTATAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTAAAACCTTtc                                             >  2:81678/1‑141 (MQ=255)
                                                                                              ttttttATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTAAAACCTTtct                                            >  2:135447/1‑141 (MQ=255)
                                                                                                    tttCTAAAAACTACTTTCCAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTAAAACCTTTCTCGAAAg                                      <  1:364174/140‑1 (MQ=255)
                                                                                                    aTTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGTTATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTAAAACCTTTCTCGAAAg                                      >  2:283244/1‑141 (MQ=255)
                                                                                                    aTTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGTTATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTAAAACCTTTCTCGAAAg                                      >  2:283296/1‑141 (MQ=255)
                                                                                                              ctactTTCTAAACACCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTAAAACCTTTCTCGAAAGCTTCTTCCAt                            <  1:358694/141‑1 (MQ=255)
                                                                                                               tactTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTAAAACCTTTCTCGAAAGCTTCTTCCAtt                           >  1:188166/1‑141 (MQ=255)
                                                                                                                             ccATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTAAAACCTTTCTCGAAAGCTTCTTCCATTTGACTAATAGTAAt             >  1:4133/1‑141 (MQ=255)
                                                                                                                                  cATCTGAACGTTATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTAAAACCTTTCTCGAAAGCTTCTTCCATTTGACTAATAGTAATGTGtt        <  1:283244/141‑1 (MQ=255)
                                                                                                                                  cATCTGAACGTTATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTAAAACCTTTCTCGAAAGCTTCTTCCATTTGACTAATAGTAATGTGtt        <  1:283296/141‑1 (MQ=255)
                                                                                                                                      tGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTCCTTAAAATCTTTCTCGAAAGCTTCTTCCATTTGACTAATAGCAATGTGTTCATc    <  1:191369/141‑1 (MQ=255)
                                                                                                                                        aaCGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTAACACCTTTCTCGAAAGATTCTTCCCCTTGACTAATAGTAATGTTTTCATCTg  <  2:274332/141‑1 (MQ=255)
                                                                                                                                        aaCGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTAAAACCTTTCTCGAAAGCTTCTTCCATTTGACTAATAGTAATGTGTTCATCTg  <  1:340802/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                        
ATCGCAAAGCAATGCGTAAGTATAAAAGAAATCAAAGAAGAAAATAGATAAAAAAACGGAAGCACTTGTAGGTAAAATAGTCTACGTGCTTCCATTTTTTATTCTAAAAACTACTTTCTAAACATCCATTCATCTGAACGATATTTTTCAGTTAATTCTTCCACTTCTGCCAATTGAGCTTCTGTTAATTCAAGTGGCTTAAATTCTATATTTAAACCTTTCTTAAAACCTTTCTCGAAAGCTTCTTCCATTTGACTAATAGTAATGTGTTCATCTG  >  CP000730/1647524‑1647800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑