BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,913	C→G	32.6%	S213S (TCG→TCC)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,913	0	C	G	32.6%	155.7 / 144.9	145	S213S (TCG→TCC)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base C (40/57); new base G (25/22); total (66/79)
Fisher's exact test for biased strand distribution p-value = 2.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.60e-01

Only 100 of 151 total aligned reads displayed.

CTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTC  >  CP000730/35773‑36053

cTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGc                                                                                                                                               <  2:208903/141‑1 (MQ=255)

     aCAAGAGCTAAAATAAAATTGTCATCTAATAAAAATAGACAGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATCTATCATTGGAGGGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGaaaa                                                                                                                                          <  1:311465/141‑1 (MQ=17)

         gagCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATTCTAATTTCGTGGCATGGCGAAAATCCg                                                                                                                                      >  2:310540/1‑141 (MQ=255)

           gCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTa                                                                                                                                    >  2:213002/1‑141 (MQ=255)

            cTAAAATAAAATTGTGATCTAATAAAAATAAAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTCATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATTGCGAAAATCCGTAg                                                                                                                                   >  2:178940/1‑141 (MQ=255)

             taaaataaaaTTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGa                                                                                                                                  >  2:187007/1‑141 (MQ=255)

                  caaaagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAag                                                                                                                           <  1:205985/128‑1 (MQ=11)

                     aagagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAACCTTTAGCTAATCACTAATATATCATGTTAGTGTTCGTTTAACTTGCTAGCATGATGATCATTTCGGGGCATGGCGAAAATCCGTAGATCTGAAgtga                                                                                                                        <  2:115899/131‑1 (MQ=2)

                      agagtattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                                                       <  2:338356/132‑1 (MQ=11)

                        tgtgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGAc                                                                                                                       <  2:108939/141‑1 (MQ=255)

                          tgATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCt                                                                                                                     <  2:283779/141‑1 (MQ=255)

                          tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAGTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                   <  2:342107/136‑1 (MQ=11)

                          tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                   <  1:81528/136‑1 (MQ=11)

                           gATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTCGATCTGTAAAGACCTg                                                                                                                    >  2:10712/1‑141 (MQ=255)

                           attttaataaAAAT‑GATGGTTCTGTTGCAAAG‑AAAAAAATATAGCTAATCACTAATATATCATGTCAGTGGACGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAAACCGTAGATATGAAGAGACCTGCg                                                                                                                  >  2:365966/5‑141 (MQ=11)

                            ttttaataaAAAT‑GCTGGTTCTGTTGCAAAGT‑AAAAAATATAGCCAACCACTAATATATCCTGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTGCGTTGCATGGCGAAAATCCGGAGATCTGAAGAGACCTGCgg                                                                                                                 <  1:321997/138‑1 (MQ=11)

                            aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                   <  2:186990/141‑1 (MQ=255)

                            aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                   <  2:10051/141‑1 (MQ=255)

                              ttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGtt                                                                                                               <  2:340933/140‑1 (MQ=11)

                               taagaaAAATTGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATCTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                                                                <  2:61688/141‑1 (MQ=255)

                                 ataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                                                                              <  2:156609/141‑1 (MQ=38)

                                                                        cacaaacacgtatatATCATGTCACTGTTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTaaa                                                                      <  2:356521/127‑1 (MQ=1)

                                                                             ccgcTAAGTTACCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGAGCGGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTAtt                                                                 <  2:1647/138‑1 (MQ=1)

                                                                                   atatATCATGTCAGTGTTCGTTTAACTTGCTAGCATGATGCTTATTTCTTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGc                                                           >  1:352814/4‑141 (MQ=1)

                                                                                              gtgtgtTGGTTTAACTTGCGAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATTTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                <  2:50975/139‑1 (MQ=11)

                                                                                              cAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                <  1:75650/141‑1 (MQ=11)

                                                                                                gttttCGTTTTACTTGCTAGCATGATGCTAATTTCGAGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                              <  1:220734/138‑1 (MQ=11)

                                                                                                gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGCGGATACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                              <  1:368075/141‑1 (MQ=11)

                                                                                                gtgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                              >  1:355741/1‑141 (MQ=11)

                                                                                                gtgtTCGTTGAAGTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                              <  2:241365/141‑1 (MQ=11)

                                                                                                 tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGA‑GAACACGGTCAAGACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                             >  1:28804/1‑141 (MQ=11)

                                                                                                 tgtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                             >  2:325923/1‑141 (MQ=17)

                                                                                                  gtTCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                            >  2:340462/1‑141 (MQ=12)

                                                                                                   ttCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGAGT‑AAATAGACTAACTACTTTTTAAAGTTATCTTTACTGTATTGATCCTTTgc                                           >  2:240599/1‑140 (MQ=11)

                                                                                                    tCGTTTAACTTGCTTGCATGATGCTAATTTCGTGGCATGGCGAAATTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                          <  2:370183/141‑1 (MQ=255)

                                                                                                    tCGTTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                          <  2:251961/141‑1 (MQ=17)

                                                                                                     cgtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATa                                         >  2:139994/4‑141 (MQ=255)

                                                                                                      gtttAACTTTCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTCATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATCGATACTTTGATat                                        <  2:157458/139‑2 (MQ=11)

                                                                                                      gtttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                        <  1:328818/139‑1 (MQ=17)

                                                                                                      gCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCCATGCCTGTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                        >  1:287963/1‑141 (MQ=255)

                                                                                                       tttAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGACAATATGTAGATTTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCGTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                       <  2:132230/140‑1 (MQ=11)

                                                                                                       gttAACTTTCTAGCATGATGTGAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                       <  2:317807/140‑1 (MQ=255)

                                                                                                       cTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGTCCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                       <  2:229755/141‑1 (MQ=17)

                                                                                                          aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATTCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGC‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                    <  1:122546/141‑1 (MQ=11)

                                                                                                          aaCTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                    >  2:183620/1‑141 (MQ=255)

                                                                                                           aCTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                   >  1:234086/1‑141 (MQ=17)

                                                                                                            cTTGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATTTCTTGtc                                  >  2:214842/1‑141 (MQ=11)

                                                                                                            cTTGCTAGCATGATGCTAATGTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtc                                  <  1:67282/141‑1 (MQ=17)

                                                                                                              tGCTAGCATGCTGCTCCTCTAGTGGAATGGCGCTAATCCGTTGCTCTGCAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                <  1:77379/141‑1 (MQ=11)

                                                                                                              tGCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctt                                >  1:6175/1‑141 (MQ=255)

                                                                                                              tGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                <  1:333939/141‑1 (MQ=17)

                                                                                                               gCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtcttt                               <  1:45719/141‑1 (MQ=11)

                                                                                                               gCTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATATTGtcttt                               >  1:159523/1‑141 (MQ=11)

                                                                                                               gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                               <  2:262409/141‑1 (MQ=17)

                                                                                                               gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACATTGtcttt                               >  1:10176/1‑141 (MQ=17)

                                                                                                               gCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCACTCCAACTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                               <  1:288150/141‑1 (MQ=11)

                                                                                                                cTAGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtatttc                              >  2:247793/1‑141 (MQ=11)

                                                                                                                 tAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                             <  1:322165/141‑1 (MQ=32)

                                                                                                                 tAGCATGATGCTAATTTCGTGGCATGAGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATAGATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctttct                             <  1:227971/141‑1 (MQ=11)

                                                                                                                  aGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATTTTGtctttctt                            >  2:118383/1‑141 (MQ=11)

                                                                                                                  aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGTTGTATTGATACTTTGATACCTTGcctttatt                            >  2:220395/1‑141 (MQ=255)

                                                                                                                   gCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGGTACTTGGATATCTTGtctttctgt                           >  1:27449/1‑139 (MQ=11)

                                                                                                                    cATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATGGATACGTTGATATCTTGTCTTTCTTAc                          >  1:307587/1‑141 (MQ=11)

                                                                                                                      tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                        >  1:374363/1‑141 (MQ=11)

                                                                                                                       gatgCTAATTTCGTGGCATGGCGAAAATCCGTAGATATCAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACttt                       >  2:32939/1‑141 (MQ=255)

                                                                                                                        atgCTAATTTCGTGGCATGGAGAAAATCCGTAGATCTGAAGAGAGCTACGGTTCTTTTGATATAGAGCGT‑AAATACATTCAATACCTTTTAAGGTATTCTTTGCCGTATTGATCATTTGATATCTCGTCTTTCTTACTTTa                      >  1:226146/1‑141 (MQ=11)

                                                                                                                          gCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAat                    >  1:64606/1‑141 (MQ=11)

                                                                                                                          gCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGGCCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATCCCTTGTCTTTTTTACTTTAat                    >  1:110179/1‑141 (MQ=255)

                                                                                                                           cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAata                   >  2:269327/1‑141 (MQ=21)

                                                                                                                            tAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCGTACTTTAatat                  >  1:232312/1‑141 (MQ=11)

                                                                                                                             aaTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTACTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATg                 >  2:243759/1‑141 (MQ=11)

                                                                                                                             aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                 >  2:230225/1‑141 (MQ=35)

                                                                                                                             aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                 >  1:358758/1‑141 (MQ=21)

                                                                                                                              aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGa                <  2:163039/141‑1 (MQ=255)

                                                                                                                              aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTAAGGCTATTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGa                >  1:352855/1‑141 (MQ=11)

                                                                                                                              aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAAtgtgt                >  1:106025/1‑140 (MQ=19)

                                                                                                                                ttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg              <  1:139994/141‑1 (MQ=255)

                                                                                                                                ttCGTGGCATGGGGAAAATCCGTAGAGCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATGCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg              <  1:127175/141‑1 (MQ=11)

                                                                                                                                ttCGTGGCATGGGCAAAGTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTTCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg              <  1:25851/141‑1 (MQ=11)

                                                                                                                                 tCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACgg             <  1:289429/141‑1 (MQ=255)

                                                                                                                                 tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg             <  1:48683/141‑1 (MQ=38)

                                                                                                                                  cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt            <  1:266827/141‑1 (MQ=255)

                                                                                                                                   gtggcgtggcGAAAATGCGTCGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGGTATTTTGCCGCCGGGTCTTTCTTACTTTAATATGACGGTg           >  2:357957/1‑141 (MQ=11)

                                                                                                                                   gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACTTACTGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTATTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg           <  1:101840/141‑1 (MQ=14)

                                                                                                                                   gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg           <  1:217799/141‑1 (MQ=255)

                                                                                                                                   gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg           <  1:243061/141‑1 (MQ=38)

                                                                                                                                    tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa          >  1:97956/1‑141 (MQ=255)

                                                                                                                                     ggCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATATAGAGCAT‑AAATACAATCAATACCTTTTAAAGTATTCTTTGCCGAATAAATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  1:69150/1‑141 (MQ=11)

                                                                                                                                     ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  2:234769/1‑141 (MQ=38)

                                                                                                                                     ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  2:85693/1‑141 (MQ=37)

                                                                                                                                     ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         >  1:43998/1‑141 (MQ=255)

                                                                                                                                     ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCCTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt         <  1:248921/141‑1 (MQ=255)

                                                                                                                                      gCATGGCGAAAATCCGTAGATCTGCAGAGACCTGCGGTTTTTTTTGT‑CAGATCCTGAAATACACTCAATACCGTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc        <  1:175336/141‑1 (MQ=14)

                                                                                                                                      gCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc        <  1:210740/141‑1 (MQ=255)

                                                                                                                                       cATGGCGAAAATCCGTAGATCTGAAGAGACCTCCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTTCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCt       <  1:250022/141‑1 (MQ=255)

                                                                                                                                       cATGGCGAAAATCCGTAGATCGGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTAGTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCt       <  1:52935/141‑1 (MQ=255)

                                                                                                                                          ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAAGATGACGGTGATCTTGc    <  1:185336/141‑1 (MQ=255)

                                                                                                                                          ggCGAAAATCCGTACATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc    <  1:27606/141‑1 (MQ=255)

                                                                                                                                            cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc  <  2:237843/141‑1 (MQ=255)

                                                                                                                                            cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTGTTATCTCGCACAT‑TAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc  <  1:269721/141‑1 (MQ=14)

CTTGAACAAGAGCTAAAATAAAATTGTGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCTAGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATATAGAGCGT‑AAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTC  >  CP000730/35773‑36053

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`