BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP000730	35,940	G→A	35.0%	R204R (CGC→CGT)	USA300HOU_0026 ←	IS431mec transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP000730	35,940	0	G	A	35.0%	182.1 / 159.5	184	R204R (CGC→CGT)	USA300HOU_0026	IS431mec transposase
Reads supporting (aligned to +/- strand): ref base G (47/72); new base A (31/33); total (78/106)
Fisher's exact test for biased strand distribution p-value = 2.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

Only 100 of 186 total aligned reads displayed.

TGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35799‑36080

tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCT‑AGCATGATGCTAGTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                                              <  2:342107/136‑1 (MQ=11)

tattttaataaAAAT‑GATGGTTCTGTTGCAAAGT‑AAAAAATATAGCTAATCACTAATATATCATGTCAGTGTTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                                              <  1:81528/136‑1 (MQ=11)

 attttaataaAAAT‑GATGGTTCTGTTGCAAAG‑AAAAAAATATAGCTAATCACTAATATATCATGTCAGTGGACGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAAACCGTAGATATGAAGAGACCTGCg                                                                                                                                             >  2:365966/5‑141 (MQ=11)

  ttttaataaAAAT‑GCTGGTTCTGTTGCAAAGT‑AAAAAATATAGCCAACCACTAATATATCCTGTCAGTGTTCGTTTAACTTGCT‑AGCATGATGCTAATTGCGTTGCATGGCGAAAATCCGGAGATCTGAAGAGACCTGCgg                                                                                                                                            <  1:321997/138‑1 (MQ=11)

  aTCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGc                                                                                                                                              <  2:10051/141‑1 (MQ=255)

     taagaaAAATTGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATCTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGt                                                                                                                                           <  2:61688/141‑1 (MQ=255)

       ataaAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTc                                                                                                                                         <  2:156609/141‑1 (MQ=38)

                                              cacaaacacgtatatATCATGTCACTGTTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTaaa                                                                                                 <  2:356521/127‑1 (MQ=1)

                                                   ccgcTAAGTTACCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGAGCGGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTAtt                                                                                            <  2:1647/138‑1 (MQ=1)

                                                         atatATCATGTCAGTGTTCGTTTAACTTGCT‑AGCATGATGCTTATTTCTTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGc                                                                                      >  1:352814/4‑141 (MQ=1)

                                                                    cAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATAc                                                                           <  1:75650/141‑1 (MQ=11)

                                                                     aGTGTTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACt                                                                          >  1:325465/1‑141 (MQ=255)

                                                                      gttttCGTTTTACTTGCT‑AGCATGATGCTAATTTCGAGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                         <  1:220734/138‑1 (MQ=11)

                                                                      gtgtTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGCGGATACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                         <  1:368075/141‑1 (MQ=11)

                                                                      gtgtTCGTTGAAGTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACtt                                                                         <  2:241365/141‑1 (MQ=11)

                                                                       tgtTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                                                        >  1:249908/1‑141 (MQ=255)

                                                                       tgtTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGAGAACACGGTCAAGACCTTTTAAAGTATTCTTTGCCGTATTGATACttt                                                                        >  1:28804/1‑141 (MQ=11)

                                                                       tgtTCGTTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACttt                                                                        >  2:325923/1‑141 (MQ=17)

                                                                        gtTCGCTTAACTTGCTCGGCATGGT‑TTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTg                                                                       >  2:265240/1‑141 (MQ=11)

                                                                         ttCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAACATCCGCAGGTGTGAAAAGGCCTGCGGTTCTTATTATATCAGA‑CACCAAAACACTCCATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGa                                                                      >  2:113727/1‑141 (MQ=11)

                                                                         ttCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGAGTAAATAGACTAACTACTTTTTAAAGTTATCTTTACTGTATTGATCCTTTgc                                                                      >  2:240599/1‑140 (MQ=11)

                                                                          tCGTTTAACTTGCT‑TGCATGATGCTAATTTCGTGGCATGGCGAAATTCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGAt                                                                     <  2:370183/141‑1 (MQ=255)

                                                                           cgtttAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATa                                                                    >  2:139994/4‑141 (MQ=255)

                                                                            gtttAACTTTCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTCATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATCGATACTTTGATat                                                                   <  2:157458/139‑2 (MQ=11)

                                                                            gtttAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                   <  1:328818/139‑1 (MQ=17)

                                                                            gCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAc                                                                   <  2:49348/141‑1 (MQ=17)

                                                                             ttgagtttGGT‑AGGATGATGCTAATTTCGTGGCATGGGGAAATTTCGTCGATCTGAAGAGACCTACGGTTCTTTTTGTAT‑TGGGCGTGAATACATTTGATTCCGTTTTAAGTATGCTTTGCGGTGTTGCTACTTTGATatc                                                                  <  2:148162/135‑3 (MQ=2)

                                                                             cTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGTCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATAcc                                                                  <  2:229755/141‑1 (MQ=17)

                                                                                aaCTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATTCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGCAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTg                                                               <  1:122546/141‑1 (MQ=11)

                                                                                 aCTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                              >  1:282719/1‑141 (MQ=32)

                                                                                 aCTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGt                                                              >  1:234086/1‑141 (MQ=17)

                                                                                  cTTGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATTTCTTGtc                                                             >  2:214842/1‑141 (MQ=11)

                                                                                   ttGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtct                                                            <  2:320869/141‑1 (MQ=17)

                                                                                    tGCT‑AGCATGCTGCTCCTCTAGTGGAATGGCGCTAATCCGTTGCTCTGCAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctt                                                           <  1:77379/141‑1 (MQ=11)

                                                                                    tGCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtctt                                                           >  1:6175/1‑141 (MQ=255)

                                                                                     gCT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtcttt                                                          <  1:45719/141‑1 (MQ=11)

                                                                                     gCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                          <  2:106598/141‑1 (MQ=17)

                                                                                     gCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                          <  2:262409/141‑1 (MQ=17)

                                                                                     gCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACATTGtcttt                                                          >  1:10176/1‑141 (MQ=17)

                                                                                     gCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCACTCCAACTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                          <  1:288150/141‑1 (MQ=11)

                                                                                      gctggcacggcgCTAAGTTCGTGCCATGGCGTAAATCCGTATATCTGAAGCGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACCCCCTCCCTTTTATCGGAGTCTTTGCTGTATTGATACTTTGATACCTTGtcttt                                                          <  2:185823/130‑1 (MQ=11)

                                                                                      cT‑AGCATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGtatttc                                                         >  2:247793/1‑141 (MQ=11)

                                                                                       t‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtctttct                                                        <  1:322165/141‑1 (MQ=32)

                                                                                         aGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGtcttactt                                                       >  2:250530/1‑141 (MQ=17)

                                                                                           cATGATGCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATGGATACGTTGATATCTTGTCTTTCTTAc                                                     >  1:307587/1‑141 (MQ=11)

                                                                                             tgatgCTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACtt                                                   >  1:354009/1‑141 (MQ=255)

                                                                                               atgCTAATTTCGTGGCATGGAGAAAATCCGTAGATCTGAAGAGAGCTACGGTTCTTTTGATAT‑AGAGCGTAAATACATTCAATACCTTTTAAGGTATTCTTTGCCGTATTGATCATTTGATATCTCGTCTTTCTTACTTTa                                                 >  1:226146/1‑141 (MQ=11)

                                                                                                tgCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTCCTTACTTTaa                                                <  1:270660/141‑1 (MQ=25)

                                                                                                 gCTAATTTCGTGGCATGGCGAAAATCAGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTatt                                               >  2:230869/1‑139 (MQ=19)

                                                                                                  cTAATTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAata                                              <  1:40514/141‑1 (MQ=255)

                                                                                                  cTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATAAACTCAATACCTTTTAAAGTATACTTTGCTGTATTGTTACTTTGATACCTTGTCTTTCTTACTTTAatt                                              >  2:340586/1‑140 (MQ=255)

                                                                                                    aaTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATg                                            >  1:358758/1‑141 (MQ=21)

                                                                                                     aTTTCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGa                                           >  1:166263/1‑141 (MQ=11)

                                                                                                     aTTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAAtgtgt                                           >  1:106025/1‑140 (MQ=19)

                                                                                                      tttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGAc                                          <  1:359891/141‑1 (MQ=11)

                                                                                                       ttCGTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                         <  1:139994/141‑1 (MQ=255)

                                                                                                       ttCGTGGCATGGGGAAAATCCGTAGAGCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATGCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACg                                         <  1:127175/141‑1 (MQ=11)

                                                                                                        tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                        <  1:340462/141‑1 (MQ=38)

                                                                                                        tCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACgg                                        <  1:48683/141‑1 (MQ=38)

                                                                                                         cGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGt                                       <  1:266827/141‑1 (MQ=255)

                                                                                                          gtggcgtggcGAAAATGCGTCGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGGTATTTTGCCGCCGGGTCTTTCTTACTTTAATATGACGGTg                                      >  2:357957/1‑141 (MQ=11)

                                                                                                          gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACTTACTGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTATTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg                                      <  1:101840/141‑1 (MQ=14)

                                                                                                          gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg                                      >  1:231267/1‑141 (MQ=14)

                                                                                                          gTGGCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTg                                      <  1:217799/141‑1 (MQ=255)

                                                                                                          gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                      <  1:239946/141‑1 (MQ=255)

                                                                                                          gTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTg                                      <  1:243061/141‑1 (MQ=38)

                                                                                                           tGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                     >  2:303568/1‑141 (MQ=255)

                                                                                                           gggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGa                                     <  2:334242/140‑1 (MQ=255)

                                                                                                            ggCATGGGGAAAATCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCATAAATACAATCAATACCTTTTAAAGTATTCTTTGCCGAATAAATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  1:69150/1‑141 (MQ=11)

                                                                                                            ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  2:234769/1‑141 (MQ=38)

                                                                                                            ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  1:65540/1‑141 (MQ=255)

                                                                                                            ggCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGAt                                    >  2:85693/1‑141 (MQ=37)

                                                                                                             gCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATc                                   <  1:210740/141‑1 (MQ=255)

                                                                                                              cATGGCGAAAATCCGTAGATCGGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTAGTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCt                                  <  1:52935/141‑1 (MQ=255)

                                                                                                                 ggggAAAATCCGTAGATCTGAAGAGACCTACGGTTCTATTTATAC‑AGTGCGCAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                               <  1:29009/138‑1 (MQ=14)

                                                                                                                 ggCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAAGATGACGGTGATCTTGc                               <  1:185336/141‑1 (MQ=255)

                                                                                                                 ggCGAAAATCCGTACATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGc                               <  1:27606/141‑1 (MQ=255)

                                                                                                                   cGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTc                             <  2:237843/141‑1 (MQ=255)

                                                                                                                     aaaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCaa                           <  1:111954/141‑1 (MQ=255)

                                                                                                                      aaaTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACAATCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAAt                          >  1:248456/1‑141 (MQ=255)

                                                                                                                       aaTCCGTAGATCTGAAGAGAACTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTGTCTTACTGTAATATGACGATGATCTTGCTCAATg                         >  1:225728/1‑141 (MQ=255)

                                                                                                                        aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATGCTTTGATCTCTTGTCTTCCTTCCTCTCATCTATCGGTGATCTTGCTCAATGa                        >  1:67203/1‑141 (MQ=11)

                                                                                                                        aTCCGTAGATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGTGCTTGCTCAAtag                        >  1:250038/1‑139 (MQ=11)

                                                                                                                        aTCCGTAGATCTGAAGAGACCTACGGATCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTACTATGACGGTGATCTAGCTCAATGa                        <  1:273391/141‑1 (MQ=14)

                                                                                                                         tCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAg                       <  2:276054/141‑1 (MQ=255)

                                                                                                                           cGTAGATCTGAAGAGACCTGCTGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     <  2:224273/141‑1 (MQ=255)

                                                                                                                           cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     >  2:121985/1‑141 (MQ=38)

                                                                                                                           cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     >  1:97327/1‑141 (MQ=255)

                                                                                                                           cGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGt                     >  1:135150/1‑141 (MQ=255)

                                                                                                                               gATCTGAAGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTAtt                 <  2:209556/141‑1 (MQ=255)

                                                                                                                                    gaggagaCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat            <  2:190055/138‑1 (MQ=255)

                                                                                                                                    gAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGat            >  1:276089/1‑141 (MQ=38)

                                                                                                                                     aaGAGACCTACGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGata           <  2:364571/141‑1 (MQ=255)

                                                                                                                                       gagaCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGGTGTATTGATACTTTGATACCTTGTCTTTCTTGCTTTGATATGACGGTGGTCTTGGTAAATGAGGTTATTCAGATAtt         >  1:191916/1‑141 (MQ=11)

                                                                                                                                       gagaCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATAtt         <  2:284436/141‑1 (MQ=38)

                                                                                                                                         gaCCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTTTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTc       >  1:143411/1‑141 (MQ=255)

                                                                                                                                          aCCTACGGTTCTTTTTATAT‑CGAGAGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTGATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCg      <  2:178502/141‑1 (MQ=14)

                                                                                                                                           ccTGCGGTTCTTTTTATAT‑AGAGCGTAAATACACTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa     <  2:118876/141‑1 (MQ=255)

                                                                                                                                           ccTACGCTTCTTTTTATAT‑AGCGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCCGTATTCATACTTTGATATCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGa     <  1:184784/141‑1 (MQ=11)

                                                                                                                                            cTGCGGTTCTTTTTATAT‑AGAGCGTAAATAAACTCAATACCTTTTAAAGTATTCTTTGCGGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGAt    >  2:217246/1‑141 (MQ=255)

TGATCTAATAAAAATAGAAGGTTCTGTTGCAAAGTAAAAAAATATAGCTAACCACTAATTTATCATGTCAGTGTTCGCTTAACTTGCT‑AGCATGATGCTAATTTCGTGGCATGGCGAAAATCCGTAGATCTGAAGAGACCTGCGGTTCTTTTTATAT‑AGAGCGTAAATACATTCAATACCTTTTAAAGTATTCTTTGCTGTATTGATACTTTGATACCTTGTCTTTCTTACTTTAATATGACGGTGATCTTGCTCAATGAGGTTATTCAGATATTTCGATGT  >  CP000730/35799‑36080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`