Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP000730 2,343,438 C→T 61.5% intergenic (+114/+250) USA300HOU_2200 → / ← alsD hypothetical protein/acetolactate decarboxylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0007302,343,4380CT61.5% 11.7 / 22.4 26intergenic (+114/+250)USA300HOU_2200/alsDhypothetical protein/acetolactate decarboxylase
Reads supporting (aligned to +/- strand):  ref base C (7/3);  new base T (8/8);  total (15/11)
Fisher's exact test for biased strand distribution p-value = 4.28e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.10e-01

AAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATTAT  >  CP000730/2343308‑2343576
                                                                                                                                  |                                                                                                                                          
aaaTTGTAGTTAAATAAGAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAAATATTGTTGTTTTCTTCTCGTAGTAACAGATTGGACTATACAATAGTAAAt                                                                                                                                  >  2:168736/1‑141 (MQ=255)
             ataataatTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAgtgt                                                                                                                     >  1:9553/1‑141 (MQ=255)
                 ttatTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAATTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTAc                                                                                                                 <  2:94058/139‑1 (MQ=255)
                 taatTTGAAAATTGTTTTATAGGGCAAGCGCGGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAGATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTAc                                                                                                                 <  2:73176/141‑1 (MQ=255)
                 aaatTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTAc                                                                                                                 <  1:133213/140‑1 (MQ=255)
                  aatTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTAcc                                                                                                                >  2:85795/1‑141 (MQ=255)
                     ttGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACcaca                                                                                                             <  2:9553/141‑1 (MQ=255)
                       gAAAATAGTTTTATAGGGCCAGCGCTGAAACATTGGCGTTCTTTTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAAt                                                                                                           <  2:166819/141‑1 (MQ=255)
                              gTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATGATAGTAAATTTTATATGAGTGTGTACCACAATTTATAAt                                                                                                    >  1:167990/1‑141 (MQ=255)
                                tttATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGGGTGTACCACAATTTATAATCt                                                                                                  >  1:29602/1‑141 (MQ=255)
                                        gCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTATCGTAGTAACAGATTGGCCTGTTTCGTGTGTAATAGTTTATGAGCGTGTACTACAATGTATAATCTAAACATaa                                                                                          >  1:269522/1‑141 (MQ=255)
                                            gcgcTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGc                                                                                      >  1:56404/1‑141 (MQ=255)
                                                 gAAAAATTGGCGGTTGTCTTTTTAGTTATTGAACTTGTAAATGATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAAtc                                                                                 <  2:355286/141‑1 (MQ=255)
                                                              ttGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGa                                                                    <  1:126639/141‑1 (MQ=255)
                                                                 tCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTGTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAACCTCAAATATCTGGAAcc                                                                 <  1:362623/141‑1 (MQ=255)
                                                                  cTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCt                                                                <  1:50231/141‑1 (MQ=255)
                                                                                          ttattGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTaa                                        >  2:286364/1‑141 (MQ=255)
                                                                                           tattGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAAt                                       <  1:58638/141‑1 (MQ=255)
                                                                                                         tctcGTAGTAACAGATTGGCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTACTAGTGAAGGGatttt                         >  1:98476/1‑140 (MQ=255)
                                                                                                           tcGTAGTAACAGATTGGCCTATATAATAGTAAATTTCATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCGTCACTACTGCTAATAGTGAAGGGatttatt                       >  2:125338/1‑141 (MQ=255)
                                                                                                               agtaACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATCTCAGGAACCTAATAAATCCAATAACTACTGCTAATAGTGAAGGGAtttattttat                   >  1:329115/1‑141 (MQ=255)
                                                                                                                          ggCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGt        >  2:97869/1‑141 (MQ=255)
                                                                                                                           gCCTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTTCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTa       <  2:2733/141‑1 (MQ=255)
                                                                                                                            ccTATATAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTaa      >  2:227269/1‑141 (MQ=255)
                                                                                                                            ccTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCACATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTCATTTTATTACTTAgcagtta      >  1:174110/1‑135 (MQ=255)
                                                                                                                                aaCAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCGAATCTCAAATATCTGGAACCTAATAAATCACCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATtat  >  2:190995/2‑141 (MQ=255)
                                                                                                                                  |                                                                                                                                          
AAATTGTAGTTAAATAATAATTTGAAAATAGTTTTATAGGGCAAGCGCTGAAAAATTGGCGTTTGTCTTTTTAGTTATTGAACTTGTAAATTATTGTTGTTTGCTTCTCGTAGTAACAGATTGGCCTATACAATAGTAAATTTTATATGAGTGTGTACCACAATTTATAATCTAAACATAACAGCAAATCTCAAATATCTGGAACCTAATAAATCCCCTCACTACTGCTAATAGTGAAGGGATTTATTTTATTACTTAGACGTAATTAT  >  CP000730/2343308‑2343576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: